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The map label for this gene is thrA [C]

Identifier: 220917217

GI number: 220917217

Start: 2362148

End: 2363545

Strand: Direct

Name: thrA [C]

Synonym: A2cp1_2115

Alternate gene names: 220917217

Gene position: 2362148-2363545 (Clockwise)

Preceding gene: 220917216

Following gene: 220917219

Centisome position: 46.97

GC content: 67.24

Gene sequence:

>1398_bases
ATGATCAGCGTCGAGAAGATCGGCGGCACCTCGATGTCCCGCTTCGACGAGGTGCTGCGCAACATCATGCTGCGCGACCC
GAGCCGCGTGTACGGCCGGGTCTACGTCGTCTCCGCGTACGCCGGCGTCACCAACCAGCTCCTCGAGCACAAGAAGACCG
GCGAGCCCGGCGTCTACGCGCGCTTCGCGAACGGCGGCGACTACACCAGCGCCATCGAGGCGCTCACCGGCAAGCTGAAG
GCCATCAACGCCGGCCTCGCGCCGCTCGGGCTCGACCTGGCCCGCGCCGACGGGTTCATCGAGAAGCGCATGAAGGAGCT
GCGCGCGCTGCTCGACTCGATGCGCCACGTGCTCGCGAGCGGCTACGTGCGCCGCGACAGCGTGCTGCTCGCCGCGCGCG
AGATGCTCGCGGCGGTGGGCGAGGCGCACAGCGCGTTCAACAGCGTGGAGCTCCTCCGGGCGAAGGACGTCGAGGCCTCC
CTGATGGACCTCTCCGGCTTCGACGACGACGACCCGCTCACCATCGACGAGCGCATCCACAAGTGCTTCACCGGCGTCGA
CGTGACCCGTCAGGTGGTGGTGGTCACCGGGTACACGAAGGGCGTCGAGGGCATCATGCGGGAGTTCGACCGCGGGTACT
CCGAGGTCACCTTCAGCAAGGTCGCCGTCGAGCTGAAGGCCGACGAGGCGGTCATCCACAAGGAGTACCACCTGTCCTCG
GCCGACCCGGAGCTGGTCGGGGCGCAGAACACGGTGGTGGTCGGGATGACCAACTACGACGTGGCCGACCAGCTCGCCGA
CGTCGGGATGGAGGCGATCCACCCGAAGGCCGCGAAGCCCATGGAGCTCGCCGGCATCGCCATCCGCCTGAAGAACACGT
TCGAGCCGGAGCACCCCGGCACGCTCATCACCAAGGACTACGTGGGCAGCCAGGCCCGCATCGAGGTGATCGCCGGCTCA
CCGAAGGTGAGCGCGGTGGAGATCCACGATCCGTCGATGGTCGGGACGGTGGGCTTCGATCTCGGGGTGATGGAGATCTT
CCAGCGCCACGGGATCTCGTACATCCTGAAGGCCACCAACGCGAACTCGATCACGCACGTGGTCTGGGAGAAGTCGATCT
CCTCGGCGTTCCTGGAGGAGCTCGAGAACCAGTACCAGAAGGTGACGGTGGTCCCGTCCGCCATCGTCTGCGTCATCGGG
AGCAACATCGCCTTCCCGGGGGTGCTGGCCCGCGCCACCCAGGCGCTCGCCGAGAACGGCATCAACGTGAACGCGTTCTC
GCAGTCGCTCCGGCAGACCAACATGCAGTTCGTGATCGACCGCAAGGACTACAAGCAGGCGGTGATCGCGCTGAACCGGG
CGCTCTGCCTGAACCCGCCGACGCAGGCGGTGGCGTAG

Upstream 100 bases:

>100_bases
CCGGGCGGGTGGCATGTGGCGCACGTTTGGGTATCATCGCGGACCGTCCATCCCAGGCCACGAGTCCCGCACCGCCACCC
AGTCCCCCGGAACCCTTCGC

Downstream 100 bases:

>100_bases
GGAGGCTGCCGGCGCCGCCGCCCGTGCGGGCAGCGGCCCGGGCCAGGCTCACGGCTCCAGCGCGCCCTCGTCCACGAGCC
GCGACACCGCCTCGATGTCG

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MISVEKIGGTSMSRFDEVLRNIMLRDPSRVYGRVYVVSAYAGVTNQLLEHKKTGEPGVYARFANGGDYTSAIEALTGKLK
AINAGLAPLGLDLARADGFIEKRMKELRALLDSMRHVLASGYVRRDSVLLAAREMLAAVGEAHSAFNSVELLRAKDVEAS
LMDLSGFDDDDPLTIDERIHKCFTGVDVTRQVVVVTGYTKGVEGIMREFDRGYSEVTFSKVAVELKADEAVIHKEYHLSS
ADPELVGAQNTVVVGMTNYDVADQLADVGMEAIHPKAAKPMELAGIAIRLKNTFEPEHPGTLITKDYVGSQARIEVIAGS
PKVSAVEIHDPSMVGTVGFDLGVMEIFQRHGISYILKATNANSITHVVWEKSISSAFLEELENQYQKVTVVPSAIVCVIG
SNIAFPGVLARATQALAENGINVNAFSQSLRQTNMQFVIDRKDYKQAVIALNRALCLNPPTQAVA

Sequences:

>Translated_465_residues
MISVEKIGGTSMSRFDEVLRNIMLRDPSRVYGRVYVVSAYAGVTNQLLEHKKTGEPGVYARFANGGDYTSAIEALTGKLK
AINAGLAPLGLDLARADGFIEKRMKELRALLDSMRHVLASGYVRRDSVLLAAREMLAAVGEAHSAFNSVELLRAKDVEAS
LMDLSGFDDDDPLTIDERIHKCFTGVDVTRQVVVVTGYTKGVEGIMREFDRGYSEVTFSKVAVELKADEAVIHKEYHLSS
ADPELVGAQNTVVVGMTNYDVADQLADVGMEAIHPKAAKPMELAGIAIRLKNTFEPEHPGTLITKDYVGSQARIEVIAGS
PKVSAVEIHDPSMVGTVGFDLGVMEIFQRHGISYILKATNANSITHVVWEKSISSAFLEELENQYQKVTVVPSAIVCVIG
SNIAFPGVLARATQALAENGINVNAFSQSLRQTNMQFVIDRKDYKQAVIALNRALCLNPPTQAVA
>Mature_465_residues
MISVEKIGGTSMSRFDEVLRNIMLRDPSRVYGRVYVVSAYAGVTNQLLEHKKTGEPGVYARFANGGDYTSAIEALTGKLK
AINAGLAPLGLDLARADGFIEKRMKELRALLDSMRHVLASGYVRRDSVLLAAREMLAAVGEAHSAFNSVELLRAKDVEAS
LMDLSGFDDDDPLTIDERIHKCFTGVDVTRQVVVVTGYTKGVEGIMREFDRGYSEVTFSKVAVELKADEAVIHKEYHLSS
ADPELVGAQNTVVVGMTNYDVADQLADVGMEAIHPKAAKPMELAGIAIRLKNTFEPEHPGTLITKDYVGSQARIEVIAGS
PKVSAVEIHDPSMVGTVGFDLGVMEIFQRHGISYILKATNANSITHVVWEKSISSAFLEELENQYQKVTVVPSAIVCVIG
SNIAFPGVLARATQALAENGINVNAFSQSLRQTNMQFVIDRKDYKQAVIALNRALCLNPPTQAVA

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=475, Percent_Identity=23.5789473684211, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 50545; Mature: 50545

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISVEKIGGTSMSRFDEVLRNIMLRDPSRVYGRVYVVSAYAGVTNQLLEHKKTGEPGVYA
CCEECCCCCCCHHHHHHHHHHHHCCCCHHHHEEEEEEEEHHHHHHHHHHHCCCCCCCCEE
RFANGGDYTSAIEALTGKLKAINAGLAPLGLDLARADGFIEKRMKELRALLDSMRHVLAS
EECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYVRRDSVLLAAREMLAAVGEAHSAFNSVELLRAKDVEASLMDLSGFDDDDPLTIDERIH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEHHHHHH
KCFTGVDVTRQVVVVTGYTKGVEGIMREFDRGYSEVTFSKVAVELKADEAVIHKEYHLSS
HHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCHHHEEHHHEEEEEECCHHHHHHHHCCCC
ADPELVGAQNTVVVGMTNYDVADQLADVGMEAIHPKAAKPMELAGIAIRLKNTFEPEHPG
CCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHCCCCCCCCCCCEEEEEEEECCCCCCCCC
TLITKDYVGSQARIEVIAGSPKVSAVEIHDPSMVGTVGFDLGVMEIFQRHGISYILKATN
CEEEHHHCCCCEEEEEEECCCCEEEEEECCCCEEEECCCHHHHHHHHHHCCCEEEEEECC
ANSITHVVWEKSISSAFLEELENQYQKVTVVPSAIVCVIGSNIAFPGVLARATQALAENG
CCCEEHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHCC
INVNAFSQSLRQTNMQFVIDRKDYKQAVIALNRALCLNPPTQAVA
CCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCHHCCC
>Mature Secondary Structure
MISVEKIGGTSMSRFDEVLRNIMLRDPSRVYGRVYVVSAYAGVTNQLLEHKKTGEPGVYA
CCEECCCCCCCHHHHHHHHHHHHCCCCHHHHEEEEEEEEHHHHHHHHHHHCCCCCCCCEE
RFANGGDYTSAIEALTGKLKAINAGLAPLGLDLARADGFIEKRMKELRALLDSMRHVLAS
EECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYVRRDSVLLAAREMLAAVGEAHSAFNSVELLRAKDVEASLMDLSGFDDDDPLTIDERIH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEHHHHHH
KCFTGVDVTRQVVVVTGYTKGVEGIMREFDRGYSEVTFSKVAVELKADEAVIHKEYHLSS
HHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCHHHEEHHHEEEEEECCHHHHHHHHCCCC
ADPELVGAQNTVVVGMTNYDVADQLADVGMEAIHPKAAKPMELAGIAIRLKNTFEPEHPG
CCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHCCCCCCCCCCCEEEEEEEECCCCCCCCC
TLITKDYVGSQARIEVIAGSPKVSAVEIHDPSMVGTVGFDLGVMEIFQRHGISYILKATN
CEEEHHHCCCCEEEEEEECCCCEEEEEECCCCEEEECCCHHHHHHHHHHCCCEEEEEECC
ANSITHVVWEKSISSAFLEELENQYQKVTVVPSAIVCVIGSNIAFPGVLARATQALAENG
CCCEEHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHCC
INVNAFSQSLRQTNMQFVIDRKDYKQAVIALNRALCLNPPTQAVA
CCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]