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The map label for this gene is thiDE [H]

Identifier: 220917019

GI number: 220917019

Start: 2154079

End: 2154864

Strand: Reverse

Name: thiDE [H]

Synonym: A2cp1_1915

Alternate gene names: 220917019

Gene position: 2154864-2154079 (Counterclockwise)

Preceding gene: 220917021

Following gene: 220917016

Centisome position: 42.85

GC content: 76.46

Gene sequence:

>786_bases
ATGAAGGTCGCCCTCACCATCGCGGGCTCGGACTCCGGCGGCGGCGCCGGCATCCAGGCCGACCTCCGCACCTTCGCCGC
CCACCGGCTCCACGGCACCTCCGCCATCACCGCCGTGACCGCGCAGAACTCGGTGGCGGTGACCGCCTGGGTGGCGCTCG
AGCCGGCCATGGTGGTCGCGCAGCTCGAGGCGGTCGCGACGGACATGCCGGTCGCGGCCACCAAGACCGGCATGCTCGCG
AACGCGGCCATCGTCGGCGCGGTGGCGGACGCGGCGGCGCGGCTGCCGCTCGGGCCGCTGGTGGTGGACCCGGTGATGGT
CGCGAAGAGCGGCGACCGGCTGCTCGACGCGGCCGCGGAGCGCGCCTACGTGGAGCGGCTCTTCCCGCTCGCCGCCATCG
TCACGCCCAACCTGCACGAGGCCGAGGCGCTCCTGGGCCGGCCGGTGCGCGGGCTCGCGGCGATGCGCGAGGCGGCCCGC
GACCTCCACCGGCTGGGCGCGCGCGCGGTGCTGGTGAAGGGCGGCGAGAGCGTGCCGGGCGCGGAGGACGTCTTCTTCGA
CGGATCCCGGCTGGAGGAGATCCCCGGCCCGCGCATCGACACCGCCAACGTGCACGGCACCGGCTGCACGCTCTCCGCCG
CCCTGTGCGCCCGGCTCGCGCTCGGGGACGCGCTGCTCGACGCGGTCCGCGGCGCGAAGGCCTACCTGGTCGAGGCGCTG
CGCCGCTCGTACACCGTCGGCAAGGGGCGCGGGCCGGTGGACCACCTCCACCCGCTCACCAGGTAG

Upstream 100 bases:

>100_bases
CGGGGCCGTTCCGCTCCTGCGCCGGCCCCTCGTTCGGCCCCCCCGCGCGCGGGCGCGCCGGCCGCGCGCCGGGGCGATCC
TGGGCTATGGTGCGCCCACC

Downstream 100 bases:

>100_bases
CGTCGCGCCGCCGGCTCACTCCAGCGCAGGCCTGATCATCTCCATCACTGCGCGCAGCGTGTTCTCGTCGCGCAGGATCA
CGCGGAGCCTGCCTTCCACG

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKVALTIAGSDSGGGAGIQADLRTFAAHRLHGTSAITAVTAQNSVAVTAWVALEPAMVVAQLEAVATDMPVAATKTGMLA
NAAIVGAVADAAARLPLGPLVVDPVMVAKSGDRLLDAAAERAYVERLFPLAAIVTPNLHEAEALLGRPVRGLAAMREAAR
DLHRLGARAVLVKGGESVPGAEDVFFDGSRLEEIPGPRIDTANVHGTGCTLSAALCARLALGDALLDAVRGAKAYLVEAL
RRSYTVGKGRGPVDHLHPLTR

Sequences:

>Translated_261_residues
MKVALTIAGSDSGGGAGIQADLRTFAAHRLHGTSAITAVTAQNSVAVTAWVALEPAMVVAQLEAVATDMPVAATKTGMLA
NAAIVGAVADAAARLPLGPLVVDPVMVAKSGDRLLDAAAERAYVERLFPLAAIVTPNLHEAEALLGRPVRGLAAMREAAR
DLHRLGARAVLVKGGESVPGAEDVFFDGSRLEEIPGPRIDTANVHGTGCTLSAALCARLALGDALLDAVRGAKAYLVEAL
RRSYTVGKGRGPVDHLHPLTR
>Mature_261_residues
MKVALTIAGSDSGGGAGIQADLRTFAAHRLHGTSAITAVTAQNSVAVTAWVALEPAMVVAQLEAVATDMPVAATKTGMLA
NAAIVGAVADAAARLPLGPLVVDPVMVAKSGDRLLDAAAERAYVERLFPLAAIVTPNLHEAEALLGRPVRGLAAMREAAR
DLHRLGARAVLVKGGESVPGAEDVFFDGSRLEEIPGPRIDTANVHGTGCTLSAALCARLALGDALLDAVRGAKAYLVEAL
RRSYTVGKGRGPVDHLHPLTR

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=261, Percent_Identity=46.7432950191571, Blast_Score=188, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6325378, Length=273, Percent_Identity=35.5311355311355, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324517, Length=273, Percent_Identity=38.4615384615385, Blast_Score=119, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324997, Length=274, Percent_Identity=37.2262773722628, Blast_Score=117, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 26792; Mature: 26792

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVALTIAGSDSGGGAGIQADLRTFAAHRLHGTSAITAVTAQNSVAVTAWVALEPAMVVA
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHEEEEECCCCEEEEEEEHHHHHHHHH
QLEAVATDMPVAATKTGMLANAAIVGAVADAAARLPLGPLVVDPVMVAKSGDRLLDAAAE
HHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHCHHHEECCCCHHHHHHHH
RAYVERLFPLAAIVTPNLHEAEALLGRPVRGLAAMREAARDLHRLGARAVLVKGGESVPG
HHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
AEDVFFDGSRLEEIPGPRIDTANVHGTGCTLSAALCARLALGDALLDAVRGAKAYLVEAL
CCCEEECCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
RRSYTVGKGRGPVDHLHPLTR
HHHCCCCCCCCCHHHCCCCCC
>Mature Secondary Structure
MKVALTIAGSDSGGGAGIQADLRTFAAHRLHGTSAITAVTAQNSVAVTAWVALEPAMVVA
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHEEEEECCCCEEEEEEEHHHHHHHHH
QLEAVATDMPVAATKTGMLANAAIVGAVADAAARLPLGPLVVDPVMVAKSGDRLLDAAAE
HHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHCHHHEECCCCHHHHHHHH
RAYVERLFPLAAIVTPNLHEAEALLGRPVRGLAAMREAARDLHRLGARAVLVKGGESVPG
HHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
AEDVFFDGSRLEEIPGPRIDTANVHGTGCTLSAALCARLALGDALLDAVRGAKAYLVEAL
CCCEEECCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
RRSYTVGKGRGPVDHLHPLTR
HHHCCCCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA