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The map label for this gene is purT [H]

Identifier: 220917010

GI number: 220917010

Start: 2142769

End: 2143932

Strand: Reverse

Name: purT [H]

Synonym: A2cp1_1906

Alternate gene names: 220917010

Gene position: 2143932-2142769 (Counterclockwise)

Preceding gene: 220917013

Following gene: 220917009

Centisome position: 42.63

GC content: 74.91

Gene sequence:

>1164_bases
ATGCGAAAGAAGATCCTGCTCCTCGGCTCCGGCGAGCTCGGCAAGGAGTTCACCATCGCGGCGCAGCGCCTCGGCCAGGA
GGTGATCGCCGTCGATTCCTACGATGGCGCGCCCGCGCAGCAGGTGGCCCACGCCCGCGAGGTCGTGAGCATGCTCGACG
GCGCGGCGCTCGACGCGCTGGTCGCGAAGCACCGGCCCGACGTGATCGTCCCGGAGATCGAGGCCATCCGCACCGAGCGC
CTCCAGGCGTACGAGGCGCAGGGCGTCCAGGTCGTCCCGAGCGCCCGCGCCGCCGCGGTCACGATGAACCGCCGCGCCAT
CCGCGACCTCGCCGCCCGCGAGCTCGGGCTCGCCACCGCGCGCTACGCCTACGCCTCGACGCCCGAGGCGTTCCGCACGG
CGGTCCGCGAGATCGGCCTCCCCTGCGTGGTGAAGCCGCTCATGTCCTCGTCGGGCAAGGGTCAGAGCGTGGTGCGCGCG
GAGGGCGACATGGAGGCCGCCTGGGCCTACGCGATGTCCGGCACCCGCGGCGACCTGCGCGAGGTGATCGTCGAGGAGTT
CATCTCGTTCGAGTCGGAGATCACGCTCCTCACCGTCACCCAGCGGCGCGGCGAGACGCTGTTCTGTCCGCCCATCGGCC
ACCGCCAGGAGCGCGGCGACTACCAGGAGAGCTGGCAGCCCCACCCGGTGCCGGCGCCGCTCCTCGAGGAGGCGCGGCGG
ATGGCGGCGGCGGTGACCCGCGCGCTCGGCGGCGCGGGCATCTTCGGCGTGGAGCTCTTCCTGGGGAAGGACCGGGTCTG
GTTCTCGGAGCTGTCGCCGCGGCCGCACGACACCGGGATGGTGACCCTGGCCGGCACGCAGCCGCTCGACGAGTTCGAGC
TGCACCTGCGCGCGGTGCTCGGCCTGCCCATCCCGCCCGTCACGCTGGTCCGGCCGGGCGCGAGCGCGGTGATCCTGGCG
CGCGGCACCGGCGCGCCGACGGTGCGCGGGCTGGAGGCGGCGCTCGCCGAGCCGGGGGCCGACGTGCGGATCTTCGGGAA
GCCGGCGCTGCGCCCGCACAGGCGCATGGGCGTGGCGCTCGTCGCGGGCGCGCCCGGCGACGATCCGCGCGCGCTGGTGG
AGCGGGCCAAGGCGGTCGCCGCCCGCGTCACCGTCGATCCGTAG

Upstream 100 bases:

>100_bases
GACCAGGTCGGCGACGGAGCGGGACACGGCCCCTCCCCATGCCACGCCTCCGCTCGGCGTGCGTGTACCGGAAGGGTCGC
AGCGCGTAGACTGCGCCGCC

Downstream 100 bases:

>100_bases
CGCGACGCCTGTCGCCGCGGCGACGGGCGGGGGCCGTCCGGCGCGTCGCCGCGCCCACGCGAAGTGTGTCCCGCGCGACA
CGTCGTGCGGAAAGCGCGCG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MRKKILLLGSGELGKEFTIAAQRLGQEVIAVDSYDGAPAQQVAHAREVVSMLDGAALDALVAKHRPDVIVPEIEAIRTER
LQAYEAQGVQVVPSARAAAVTMNRRAIRDLAARELGLATARYAYASTPEAFRTAVREIGLPCVVKPLMSSSGKGQSVVRA
EGDMEAAWAYAMSGTRGDLREVIVEEFISFESEITLLTVTQRRGETLFCPPIGHRQERGDYQESWQPHPVPAPLLEEARR
MAAAVTRALGGAGIFGVELFLGKDRVWFSELSPRPHDTGMVTLAGTQPLDEFELHLRAVLGLPIPPVTLVRPGASAVILA
RGTGAPTVRGLEAALAEPGADVRIFGKPALRPHRRMGVALVAGAPGDDPRALVERAKAVAARVTVDP

Sequences:

>Translated_387_residues
MRKKILLLGSGELGKEFTIAAQRLGQEVIAVDSYDGAPAQQVAHAREVVSMLDGAALDALVAKHRPDVIVPEIEAIRTER
LQAYEAQGVQVVPSARAAAVTMNRRAIRDLAARELGLATARYAYASTPEAFRTAVREIGLPCVVKPLMSSSGKGQSVVRA
EGDMEAAWAYAMSGTRGDLREVIVEEFISFESEITLLTVTQRRGETLFCPPIGHRQERGDYQESWQPHPVPAPLLEEARR
MAAAVTRALGGAGIFGVELFLGKDRVWFSELSPRPHDTGMVTLAGTQPLDEFELHLRAVLGLPIPPVTLVRPGASAVILA
RGTGAPTVRGLEAALAEPGADVRIFGKPALRPHRRMGVALVAGAPGDDPRALVERAKAVAARVTVDP
>Mature_387_residues
MRKKILLLGSGELGKEFTIAAQRLGQEVIAVDSYDGAPAQQVAHAREVVSMLDGAALDALVAKHRPDVIVPEIEAIRTER
LQAYEAQGVQVVPSARAAAVTMNRRAIRDLAARELGLATARYAYASTPEAFRTAVREIGLPCVVKPLMSSSGKGQSVVRA
EGDMEAAWAYAMSGTRGDLREVIVEEFISFESEITLLTVTQRRGETLFCPPIGHRQERGDYQESWQPHPVPAPLLEEARR
MAAAVTRALGGAGIFGVELFLGKDRVWFSELSPRPHDTGMVTLAGTQPLDEFELHLRAVLGLPIPPVTLVRPGASAVILA
RGTGAPTVRGLEAALAEPGADVRIFGKPALRPHRRMGVALVAGAPGDDPRALVERAKAVAARVTVDP

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=385, Percent_Identity=52.4675324675325, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI1786733, Length=315, Percent_Identity=26.031746031746, Blast_Score=68, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324702, Length=367, Percent_Identity=25.3405994550409, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 41466; Mature: 41466

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKILLLGSGELGKEFTIAAQRLGQEVIAVDSYDGAPAQQVAHAREVVSMLDGAALDAL
CCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHH
VAKHRPDVIVPEIEAIRTERLQAYEAQGVQVVPSARAAAVTMNRRAIRDLAARELGLATA
HHHCCCCEECCCHHHHHHHHHHHHHCCCEEEECCCCHHEEHHHHHHHHHHHHHHHHHHHH
RYAYASTPEAFRTAVREIGLPCVVKPLMSSSGKGQSVVRAEGDMEAAWAYAMSGTRGDLR
HHHHCCCCHHHHHHHHHCCCCCEEHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHH
EVIVEEFISFESEITLLTVTQRRGETLFCPPIGHRQERGDYQESWQPHPVPAPLLEEARR
HHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCCHHCCCHHHCCCCCCCCCHHHHHHHH
MAAAVTRALGGAGIFGVELFLGKDRVWFSELSPRPHDTGMVTLAGTQPLDEFELHLRAVL
HHHHHHHHHCCCCEEEEEEEECCCCEEHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
GLPIPPVTLVRPGASAVILARGTGAPTVRGLEAALAEPGADVRIFGKPALRPHRRMGVAL
CCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEECCCCCCHHHHCCEEE
VAGAPGDDPRALVERAKAVAARVTVDP
EECCCCCCHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MRKKILLLGSGELGKEFTIAAQRLGQEVIAVDSYDGAPAQQVAHAREVVSMLDGAALDAL
CCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHH
VAKHRPDVIVPEIEAIRTERLQAYEAQGVQVVPSARAAAVTMNRRAIRDLAARELGLATA
HHHCCCCEECCCHHHHHHHHHHHHHCCCEEEECCCCHHEEHHHHHHHHHHHHHHHHHHHH
RYAYASTPEAFRTAVREIGLPCVVKPLMSSSGKGQSVVRAEGDMEAAWAYAMSGTRGDLR
HHHHCCCCHHHHHHHHHCCCCCEEHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHH
EVIVEEFISFESEITLLTVTQRRGETLFCPPIGHRQERGDYQESWQPHPVPAPLLEEARR
HHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCCHHCCCHHHCCCCCCCCCHHHHHHHH
MAAAVTRALGGAGIFGVELFLGKDRVWFSELSPRPHDTGMVTLAGTQPLDEFELHLRAVL
HHHHHHHHHCCCCEEEEEEEECCCCEEHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
GLPIPPVTLVRPGASAVILARGTGAPTVRGLEAALAEPGADVRIFGKPALRPHRRMGVAL
CCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEECCCCCCHHHHCCEEE
VAGAPGDDPRALVERAKAVAARVTVDP
EECCCCCCHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA