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The map label for this gene is pfp [H]

Identifier: 220916975

GI number: 220916975

Start: 2102637

End: 2103821

Strand: Reverse

Name: pfp [H]

Synonym: A2cp1_1871

Alternate gene names: 220916975

Gene position: 2103821-2102637 (Counterclockwise)

Preceding gene: 220916980

Following gene: 220916971

Centisome position: 41.83

GC content: 67.76

Gene sequence:

>1185_bases
ATGACCAAGAAGCGCGTCGGCATCCTCACCGGCGGCGGCGACGTCCCCGGCCTGAACAGCGTCATCAAGAGCGTCGTGTA
CCGCGGCGCCGAGCACGACATCGAGGTCGTGGGCATCCGGCGCGGGTGGGAGGGGCTCACCCACCTCGACCTCACCGACT
CCGCCAGCAAGGCGCGCTACGTCTGGCCGCTCGATCGCGAGAACACCCGCACCATCGACCGGACCGGCGGCACCATCCTC
CACTCCTCGCGGACGAATCCCGCGAAGATGAAGAAGCTGCCGCCGCACCTGCAGGGGACGTCGTTCCCGACCTCGACCAG
CACCAAGGGCGGGACGGCCACCACCACCTTCGACGTCTCGAAGCAGGTGCTCGCCAACCTGGAGGCGCTCGGGCTCGACT
ACCTGCTCGCCATCGGCGGCGACGACACGCTGTCCTACGCGGCCAGGCTCCACGACCTCGGGTTCAAGGTCATCGCGATC
CCGAAGACCATGGACAACGACGTCCGGAACACCGAGTACTGCATCGGGTTCTCGACCGCGATCACCCGCGCCATGGACGC
GATCGAGCGCCAGCGCACCACGGTCGGCTCGCACGAGCGCATCGGCGTGTTCCGGGTGTTCGGCCGGGACGCGGGGTTCA
CCTCGCTCTACACCGCCTACGTCACCTCGATCCGCTGCCTCATCCCCGAGCACAAGCCGAAGATCGAGCACGTGGTGGAC
CTGCTGGTCACCGACAAGCGCGACAACCCCTCGGGCTACGCGCTCATCGTGCTCTCCGAGGGCGCGCAGTGGGAGGGCTA
CGCGGTGCAGGAGTACGGCGACCCCGACGCGTTCGGCCACCGCAAGAAGGCCAGCGTGGGCGAGTCGCTCTCCGACGAGA
TCAAGCGGCGCACCGGCGAGGAAACGATCGTCTCCGACCTGACCTACGACCTGCGCTCGGGCGATCCCGACTTCATCGAC
AAGCTCATCGCGTACACCTTCGGCACCATGGCGTTCGACGCGGTGCTGGAGGGCAAGAGCGGCCTCATGTCGGCGCTGGT
CGACGGGCGCTACGACCTCGTCCCCATCCCCGACCCGAAGCTCGGGCCCCGCAAGGTGGACGTGGCGAGCATGTACAACA
CCGACCGCCTGCGCCCGATCTACGCGAACAAGCGCGGGCTGCCGGTGTTCCTCAACAAGATCTGA

Upstream 100 bases:

>100_bases
GCGGCAAGCTGCCGCACGCGTGACCCCGGCCCCGCCCGGTCACCGGGCGTCACGCTCCGGTTTCGGGCGCGGGCGCGCGG
GCGCGGCTAGGATGCTGGCC

Downstream 100 bases:

>100_bases
CGCGACCCCCGGCGCGGCGCCCGGCCCGCGCCGCCGCGGCCGCCCGTCCTCCGGCGCGGCGCCGCGGCGCGGTCCGACCG
GCCTACAGCTCGTACAGCGA

Product: Diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MTKKRVGILTGGGDVPGLNSVIKSVVYRGAEHDIEVVGIRRGWEGLTHLDLTDSASKARYVWPLDRENTRTIDRTGGTIL
HSSRTNPAKMKKLPPHLQGTSFPTSTSTKGGTATTTFDVSKQVLANLEALGLDYLLAIGGDDTLSYAARLHDLGFKVIAI
PKTMDNDVRNTEYCIGFSTAITRAMDAIERQRTTVGSHERIGVFRVFGRDAGFTSLYTAYVTSIRCLIPEHKPKIEHVVD
LLVTDKRDNPSGYALIVLSEGAQWEGYAVQEYGDPDAFGHRKKASVGESLSDEIKRRTGEETIVSDLTYDLRSGDPDFID
KLIAYTFGTMAFDAVLEGKSGLMSALVDGRYDLVPIPDPKLGPRKVDVASMYNTDRLRPIYANKRGLPVFLNKI

Sequences:

>Translated_394_residues
MTKKRVGILTGGGDVPGLNSVIKSVVYRGAEHDIEVVGIRRGWEGLTHLDLTDSASKARYVWPLDRENTRTIDRTGGTIL
HSSRTNPAKMKKLPPHLQGTSFPTSTSTKGGTATTTFDVSKQVLANLEALGLDYLLAIGGDDTLSYAARLHDLGFKVIAI
PKTMDNDVRNTEYCIGFSTAITRAMDAIERQRTTVGSHERIGVFRVFGRDAGFTSLYTAYVTSIRCLIPEHKPKIEHVVD
LLVTDKRDNPSGYALIVLSEGAQWEGYAVQEYGDPDAFGHRKKASVGESLSDEIKRRTGEETIVSDLTYDLRSGDPDFID
KLIAYTFGTMAFDAVLEGKSGLMSALVDGRYDLVPIPDPKLGPRKVDVASMYNTDRLRPIYANKRGLPVFLNKI
>Mature_393_residues
TKKRVGILTGGGDVPGLNSVIKSVVYRGAEHDIEVVGIRRGWEGLTHLDLTDSASKARYVWPLDRENTRTIDRTGGTILH
SSRTNPAKMKKLPPHLQGTSFPTSTSTKGGTATTTFDVSKQVLANLEALGLDYLLAIGGDDTLSYAARLHDLGFKVIAIP
KTMDNDVRNTEYCIGFSTAITRAMDAIERQRTTVGSHERIGVFRVFGRDAGFTSLYTAYVTSIRCLIPEHKPKIEHVVDL
LVTDKRDNPSGYALIVLSEGAQWEGYAVQEYGDPDAFGHRKKASVGESLSDEIKRRTGEETIVSDLTYDLRSGDPDFIDK
LIAYTFGTMAFDAVLEGKSGLMSALVDGRYDLVPIPDPKLGPRKVDVASMYNTDRLRPIYANKRGLPVFLNKI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=360, Percent_Identity=25.8333333333333, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI266453768, Length=360, Percent_Identity=25.8333333333333, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI266453748, Length=360, Percent_Identity=25.8333333333333, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI4505749, Length=360, Percent_Identity=25.8333333333333, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI48762920, Length=360, Percent_Identity=25.2777777777778, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI11321601, Length=364, Percent_Identity=24.1758241758242, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1790350, Length=339, Percent_Identity=29.7935103244838, Blast_Score=131, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6321679, Length=252, Percent_Identity=23.015873015873, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323861, Length=373, Percent_Identity=22.7882037533512, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24652337, Length=388, Percent_Identity=23.7113402061856, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17647809, Length=388, Percent_Identity=23.7113402061856, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28573326, Length=384, Percent_Identity=22.9166666666667, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 43317; Mature: 43186

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKRVGILTGGGDVPGLNSVIKSVVYRGAEHDIEVVGIRRGWEGLTHLDLTDSASKARY
CCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEECCCCCCCCEE
VWPLDRENTRTIDRTGGTILHSSRTNPAKMKKLPPHLQGTSFPTSTSTKGGTATTTFDVS
EECCCCCCCCEEECCCCEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHH
KQVLANLEALGLDYLLAIGGDDTLSYAARLHDLGFKVIAIPKTMDNDVRNTEYCIGFSTA
HHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEEHHHH
ITRAMDAIERQRTTVGSHERIGVFRVFGRDAGFTSLYTAYVTSIRCLIPEHKPKIEHVVD
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHH
LLVTDKRDNPSGYALIVLSEGAQWEGYAVQEYGDPDAFGHRKKASVGESLSDEIKRRTGE
HHHCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHCCC
ETIVSDLTYDLRSGDPDFIDKLIAYTFGTMAFDAVLEGKSGLMSALVDGRYDLVPIPDPK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCC
LGPRKVDVASMYNTDRLRPIYANKRGLPVFLNKI
CCCCEEEHHHHCCCCCCCEEECCCCCCCEEEECC
>Mature Secondary Structure 
TKKRVGILTGGGDVPGLNSVIKSVVYRGAEHDIEVVGIRRGWEGLTHLDLTDSASKARY
CCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEECCCCCCCCEE
VWPLDRENTRTIDRTGGTILHSSRTNPAKMKKLPPHLQGTSFPTSTSTKGGTATTTFDVS
EECCCCCCCCEEECCCCEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHH
KQVLANLEALGLDYLLAIGGDDTLSYAARLHDLGFKVIAIPKTMDNDVRNTEYCIGFSTA
HHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEEHHHH
ITRAMDAIERQRTTVGSHERIGVFRVFGRDAGFTSLYTAYVTSIRCLIPEHKPKIEHVVD
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHH
LLVTDKRDNPSGYALIVLSEGAQWEGYAVQEYGDPDAFGHRKKASVGESLSDEIKRRTGE
HHHCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHCCC
ETIVSDLTYDLRSGDPDFIDKLIAYTFGTMAFDAVLEGKSGLMSALVDGRYDLVPIPDPK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCC
LGPRKVDVASMYNTDRLRPIYANKRGLPVFLNKI
CCCCEEEHHHHCCCCCCCEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]