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The map label for this gene is gloA [H]

Identifier: 220916949

GI number: 220916949

Start: 2065381

End: 2065767

Strand: Reverse

Name: gloA [H]

Synonym: A2cp1_1845

Alternate gene names: 220916949

Gene position: 2065767-2065381 (Counterclockwise)

Preceding gene: 220916950

Following gene: 220916946

Centisome position: 41.07

GC content: 71.32

Gene sequence:

>387_bases
GTGCGCATCCTCCACACCATGCTCCGCGTCGGCGACCTGGAGCGGTCGCTCGCCTTCTACACCGGCGTCCTCGGCATGAC
ACTGCTCCGCCGCCAGGAGTACCCCGACGGCCGGTTCACGCTCGCGTTCGTGGGCTACGGCCCGGAGTCCGAGCAGACCG
CCCTCGAGCTGACGCACAACTGGGACACGCCGCGCTACGACCTCGGCACCGGCTTCGGGCACGTGGCGCTGGAGGTGCCG
GACGCCTACGCCGCCTGCGCCGAGATCAAGGCGCGCGGCGGGCGGGTGGTGCGCGAGGCCGGCCCGATGAAGCACGGGAC
CACGGTGATCGCGTTCGTCGAGGATCCCGACGGCTACAAGGTCGAGCTCATCCAGCGCGGGAGCTGA

Upstream 100 bases:

>100_bases
CGGCGCTCTGCCCGGAGTGCCAGGCGCGCTCCGAGCCGCGCCGCAACGCGACGCGCAAGAACCTGACCGACTTCAGCTGA
CCCGCACCCCGGAGATCCCC

Downstream 100 bases:

>100_bases
GCGCGGATTCCCTCACCTCCGCTCGCCCTGAGCCTGTCGAAGGGCGAGCGGGCGGGGGGTCACGCCGCCCGGACCACCAC
GCCGGTGGCGGTCTCGGCGA

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MRILHTMLRVGDLERSLAFYTGVLGMTLLRRQEYPDGRFTLAFVGYGPESEQTALELTHNWDTPRYDLGTGFGHVALEVP
DAYAACAEIKARGGRVVREAGPMKHGTTVIAFVEDPDGYKVELIQRGS

Sequences:

>Translated_128_residues
MRILHTMLRVGDLERSLAFYTGVLGMTLLRRQEYPDGRFTLAFVGYGPESEQTALELTHNWDTPRYDLGTGFGHVALEVP
DAYAACAEIKARGGRVVREAGPMKHGTTVIAFVEDPDGYKVELIQRGS
>Mature_128_residues
MRILHTMLRVGDLERSLAFYTGVLGMTLLRRQEYPDGRFTLAFVGYGPESEQTALELTHNWDTPRYDLGTGFGHVALEVP
DAYAACAEIKARGGRVVREAGPMKHGTTVIAFVEDPDGYKVELIQRGS

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=149, Percent_Identity=37.5838926174497, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787940, Length=125, Percent_Identity=64.8, Blast_Score=179, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=27.0676691729323, Blast_Score=65, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323639, Length=142, Percent_Identity=39.4366197183099, Blast_Score=102, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20129743, Length=150, Percent_Identity=40, Blast_Score=99, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19920404, Length=138, Percent_Identity=31.8840579710145, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14201; Mature: 14201

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTMLRVGDLERSLAFYTGVLGMTLLRRQEYPDGRFTLAFVGYGPESEQTALELTHN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEC
WDTPRYDLGTGFGHVALEVPDAYAACAEIKARGGRVVREAGPMKHGTTVIAFVEDPDGYK
CCCCCEECCCCCCEEEEECCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEECCCCCE
VELIQRGS
EEEEECCC
>Mature Secondary Structure
MRILHTMLRVGDLERSLAFYTGVLGMTLLRRQEYPDGRFTLAFVGYGPESEQTALELTHN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEC
WDTPRYDLGTGFGHVALEVPDAYAACAEIKARGGRVVREAGPMKHGTTVIAFVEDPDGYK
CCCCCEECCCCCCEEEEECCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEECCCCCE
VELIQRGS
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]