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The map label for this gene is narH [H]

Identifier: 220916882

GI number: 220916882

Start: 1987709

End: 1989178

Strand: Reverse

Name: narH [H]

Synonym: A2cp1_1778

Alternate gene names: 220916882

Gene position: 1989178-1987709 (Counterclockwise)

Preceding gene: 220916883

Following gene: 220916881

Centisome position: 39.55

GC content: 72.04

Gene sequence:

>1470_bases
ATGAACGTCCGCGCGCAGATTTCGTCGGTGTTCCACCTCGACAAGTGCATCGGGTGCCACACCTGCAGCGTCGCCTGCAA
GAACCAGTGGACCGACCGGAAGGGCGTCGAGTACGCGTGGTGGAACAACGTGGAGACCCGGCCCGGCACCGGGTACCCGG
TGCTCTGGGAGGACCAGGAGCGCCACCAGGGCGGCTGGAAGGTCGAGGGCGGTGAGCTCTCGCTCCGCCAGGCCAGCCCC
GGCGCCTCGCTGGGCCGGCTGTTCCACAACCCGAACCTGCCGGTGCTCCAGGACTACTACGAGCCCTGGACGTACAAGTA
CGACGACCTGTTCACCGCGCCCGCCGGCGACGTGCAGCCCACCGCGCGGCCGGTGTCGCTGGTGGACGGCAAGCCCATCG
ACATCGCCTCCGGGCCGGCCTGGGACGACGACCTGGGCGGCTCCGGCACCTACGCCGCGAACGACCCCGGCCTGGCCGTG
CTCACCGAGGCGGAGCGGACGCAGCTGTTCGACCTCGAGCGGATGGTGTTCTTCTACCTGCCGCGCATCTGCAACCACTG
CCTGAACGCGGCCTGCGTGGCCGCCTGTCCCTCCGGCGCGATCTACAAGCGCGGGGAGGACGGCGTGGTGCTGATCTCGC
AGGACGTCTGCAAGGGCTGGCGCATGTGCGTCTCGGCCTGCCCGTACAAGAAGGTCTACTTCAACTGGGAGACGGGCAAG
TCCGAGAAGTGCATCCTGTGCTACCCGCGGCTCGAGGCGGGCGAGGCGCCGGCGTGCTTCCACACCTGCGTCGGCCGCAT
CCGCTACCTCGGCGTGCTGCTCTACGACGCCGACCGGATCGCGCAGGCCGCGTCGCGCCCGGACCAGGACCTGGTCGCGG
CGCAGCGATCGCTCATCCTCGATCCCTCCGACCCGGCGGTGATCGCGGCGGCGCGCGCCAGCGGCGTGCCGGACGCGATG
GTCGAGTCCGCCCGGCGATCGCCGGTGTACCGCTTCGTGAAGGAGTGGGAGATCGCGCTGCCGCTCCACCCCGAGTACCG
CACGTTGCCCATGCTGTTCTACGTGCCGCCGCTCCTCCCGGCGCTCGCGCGCTCGGCGTCCGATCCGGACGGCAGGTTCT
TCACCTCGCTCGAGTCGGCGCGCCTCCCGCTCCGCTACCTGGCCGGCCTGTTCGGCGGCGGGAACGAGGCGGTGGTGGAG
GCCGCCTACCGCCGGCTCATCTCGGTGCGCGTGCACCGCCGCGCCGGCCAGGTGGGCGACGTGAGCCCGGCGCAGGCCGC
GGCCGCGCTGGAGGCGGCCGGGCTCCGCGCCGAGCAGGCGGACGAGATCCACCGCATGACCACGCTCGCGCCCATGGAGG
AGCGGATCGTCATCCCGCCGATGGCGCGCGAGGCGGAGACCGAGCCGACCGTGGAGCCGCAGTCGCGCTCGGCGAGCGGG
CTCGGCTTCCTGCGCCCGCTGGGGAGGTGA

Upstream 100 bases:

>100_bases
ACTACTGGGGGCCCACCGGCAACAACCGCGACACCTACGTCCGCGTCACCAAGCTCGAGGGCGAGCCGCAGTGGTGACGG
CGGCCCGGCCGAGGACCCCC

Downstream 100 bases:

>100_bases
GCCGATGGAACGCACGCACGCCGAGCTCTGCCGCACCCTCTCCGCGCTGCTCTCCTACCCGACCGGCGACGCCGCCGCGC
TGGCGCACGAGGCGCGGGGC

Product: nitrate reductase subunit beta

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MNVRAQISSVFHLDKCIGCHTCSVACKNQWTDRKGVEYAWWNNVETRPGTGYPVLWEDQERHQGGWKVEGGELSLRQASP
GASLGRLFHNPNLPVLQDYYEPWTYKYDDLFTAPAGDVQPTARPVSLVDGKPIDIASGPAWDDDLGGSGTYAANDPGLAV
LTEAERTQLFDLERMVFFYLPRICNHCLNAACVAACPSGAIYKRGEDGVVLISQDVCKGWRMCVSACPYKKVYFNWETGK
SEKCILCYPRLEAGEAPACFHTCVGRIRYLGVLLYDADRIAQAASRPDQDLVAAQRSLILDPSDPAVIAAARASGVPDAM
VESARRSPVYRFVKEWEIALPLHPEYRTLPMLFYVPPLLPALARSASDPDGRFFTSLESARLPLRYLAGLFGGGNEAVVE
AAYRRLISVRVHRRAGQVGDVSPAQAAAALEAAGLRAEQADEIHRMTTLAPMEERIVIPPMAREAETEPTVEPQSRSASG
LGFLRPLGR

Sequences:

>Translated_489_residues
MNVRAQISSVFHLDKCIGCHTCSVACKNQWTDRKGVEYAWWNNVETRPGTGYPVLWEDQERHQGGWKVEGGELSLRQASP
GASLGRLFHNPNLPVLQDYYEPWTYKYDDLFTAPAGDVQPTARPVSLVDGKPIDIASGPAWDDDLGGSGTYAANDPGLAV
LTEAERTQLFDLERMVFFYLPRICNHCLNAACVAACPSGAIYKRGEDGVVLISQDVCKGWRMCVSACPYKKVYFNWETGK
SEKCILCYPRLEAGEAPACFHTCVGRIRYLGVLLYDADRIAQAASRPDQDLVAAQRSLILDPSDPAVIAAARASGVPDAM
VESARRSPVYRFVKEWEIALPLHPEYRTLPMLFYVPPLLPALARSASDPDGRFFTSLESARLPLRYLAGLFGGGNEAVVE
AAYRRLISVRVHRRAGQVGDVSPAQAAAALEAAGLRAEQADEIHRMTTLAPMEERIVIPPMAREAETEPTVEPQSRSASG
LGFLRPLGR
>Mature_489_residues
MNVRAQISSVFHLDKCIGCHTCSVACKNQWTDRKGVEYAWWNNVETRPGTGYPVLWEDQERHQGGWKVEGGELSLRQASP
GASLGRLFHNPNLPVLQDYYEPWTYKYDDLFTAPAGDVQPTARPVSLVDGKPIDIASGPAWDDDLGGSGTYAANDPGLAV
LTEAERTQLFDLERMVFFYLPRICNHCLNAACVAACPSGAIYKRGEDGVVLISQDVCKGWRMCVSACPYKKVYFNWETGK
SEKCILCYPRLEAGEAPACFHTCVGRIRYLGVLLYDADRIAQAASRPDQDLVAAQRSLILDPSDPAVIAAARASGVPDAM
VESARRSPVYRFVKEWEIALPLHPEYRTLPMLFYVPPLLPALARSASDPDGRFFTSLESARLPLRYLAGLFGGGNEAVVE
AAYRRLISVRVHRRAGQVGDVSPAQAAAALEAAGLRAEQADEIHRMTTLAPMEERIVIPPMAREAETEPTVEPQSRSASG
LGFLRPLGR

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=492, Percent_Identity=50.8130081300813, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI1787740, Length=492, Percent_Identity=48.780487804878, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1787872, Length=99, Percent_Identity=43.4343434343434, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787122, Length=91, Percent_Identity=43.956043956044, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI2367345, Length=107, Percent_Identity=32.7102803738318, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789370, Length=99, Percent_Identity=37.3737373737374, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI226510944, Length=88, Percent_Identity=36.3636363636364, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 53786; Mature: 53786

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVRAQISSVFHLDKCIGCHTCSVACKNQWTDRKGVEYAWWNNVETRPGTGYPVLWEDQE
CCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCH
RHQGGWKVEGGELSLRQASPGASLGRLFHNPNLPVLQDYYEPWTYKYDDLFTAPAGDVQP
HCCCCCEEECCEEEEEECCCCCHHHHHHCCCCCCHHHHHCCCCCEECCCEEECCCCCCCC
TARPVSLVDGKPIDIASGPAWDDDLGGSGTYAANDPGLAVLTEAERTQLFDLERMVFFYL
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
PRICNHCLNAACVAACPSGAIYKRGEDGVVLISQDVCKGWRMCVSACPYKKVYFNWETGK
HHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEHHHHHHHHHHHHHCCCEEEEEEECCCC
SEKCILCYPRLEAGEAPACFHTCVGRIRYLGVLLYDADRIAQAASRPDQDLVAAQRSLIL
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCCCHHHHHHHHHEEE
DPSDPAVIAAARASGVPDAMVESARRSPVYRFVKEWEIALPLHPEYRTLPMLFYVPPLLP
CCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHCEEEECCCCCCCCCCHHHHHHHHHH
ALARSASDPDGRFFTSLESARLPLRYLAGLFGGGNEAVVEAAYRRLISVRVHRRAGQVGD
HHHHCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VSPAQAAAALEAAGLRAEQADEIHRMTTLAPMEERIVIPPMAREAETEPTVEPQSRSASG
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCC
LGFLRPLGR
CCCCCCCCC
>Mature Secondary Structure
MNVRAQISSVFHLDKCIGCHTCSVACKNQWTDRKGVEYAWWNNVETRPGTGYPVLWEDQE
CCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCH
RHQGGWKVEGGELSLRQASPGASLGRLFHNPNLPVLQDYYEPWTYKYDDLFTAPAGDVQP
HCCCCCEEECCEEEEEECCCCCHHHHHHCCCCCCHHHHHCCCCCEECCCEEECCCCCCCC
TARPVSLVDGKPIDIASGPAWDDDLGGSGTYAANDPGLAVLTEAERTQLFDLERMVFFYL
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
PRICNHCLNAACVAACPSGAIYKRGEDGVVLISQDVCKGWRMCVSACPYKKVYFNWETGK
HHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEHHHHHHHHHHHHHCCCEEEEEEECCCC
SEKCILCYPRLEAGEAPACFHTCVGRIRYLGVLLYDADRIAQAASRPDQDLVAAQRSLIL
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCCCHHHHHHHHHEEE
DPSDPAVIAAARASGVPDAMVESARRSPVYRFVKEWEIALPLHPEYRTLPMLFYVPPLLP
CCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHCEEEECCCCCCCCCCHHHHHHHHHH
ALARSASDPDGRFFTSLESARLPLRYLAGLFGGGNEAVVEAAYRRLISVRVHRRAGQVGD
HHHHCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VSPAQAAAALEAAGLRAEQADEIHRMTTLAPMEERIVIPPMAREAETEPTVEPQSRSASG
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCC
LGFLRPLGR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]