Click here to switch to the map view.

The map label for this gene is dagK [H]

Identifier: 220916530

GI number: 220916530

Start: 1593655

End: 1594584

Strand: Direct

Name: dagK [H]

Synonym: A2cp1_1424

Alternate gene names: 220916530

Gene position: 1593655-1594584 (Clockwise)

Preceding gene: 220916528

Following gene: 220916531

Centisome position: 31.69

GC content: 74.73

Gene sequence:

>930_bases
GTGAAGCCCTTCCTGATCGTCAACCCCGCCAGCGCCTCCGGCCGCACCGGCCGCCACTTCGACCGGATCGCCCGCGCGGT
GCGCGCCTCCATCGGCGACTTCGAGTGCGCCTTCACCCGCGCCCGCGGCGACGGCGTGCGCCTGGCGCGCGAGGCGCTCG
CCTCGGGCGGCAAGCTGGTGGTGGCGGTCGGCGGCGACGGCACCGCCAGCGAGGTCATCGACGGGCTCACCGGCGAGACC
GGCCCGCGCGATCCCGAGGCGCACTTCGGGTTCATCCCCCGCGGCACCGGCGGCGACCTGTGCCGCACGCTCGGCATCGA
CCCCGACGTGGACTCGGCGGCGCGCACGCTCGCCTCGCGCGACGTGGCGGTGCTCGATCTGGGGAGGCTGGAGCTGGCGG
GCCCCGACGGCGCGCCGGTCACGCGTCACTTCGCCAACGTGGCCGGCTTCGGCGTGTCGGGCGAGGTGAGCGCGCTCGTG
AACCGCGGGCTGAAGCTCCCCAGCGGGAAGCTGTCCTACATGCTCGCCTCCGCGCGCGCGCTCATGAGCTGGTCGGACCA
GCCGGTGCGGTGGCGCGTGGACGGCGGGGCGTGGAAGGAGGAGCGGATCACCGCCGTCTCGGTCTGCAACGGGCGCTACT
TCGGGGGCGGCATGAAGGTGGCGCCGGACGCCCGCATGGACGACGGCCTGTTCGACGTCGTGCTGTGGAAGGCGTTCGGC
ATGGGCGACTTCATCGCCAAGCGCCCCATGCTCTACGACGGCACCCACGTGCGCCTCGAGAACACCCGGGTGCTGCGCGC
GCGCACGCTGGAGGCCGAGCCGCTGGAGGGGGCGCGGGTGCGCATCGACTCCGACGGCGAGTCGCCGGGGCGCCTCCCGG
CGCGGTTCACGATCCTCCCGGGTGCGCTCCGGATCCGCGTGGGGCGTTGA

Upstream 100 bases:

>100_bases
GGCCCGTGCCAGGAGGGGCGCCGCGGAAGGCGTGCCGCGCGACAGGTTCGTGACGCGGCAGGACGCCGCGGGGACCCGTC
CCGGGATATGATCCGGTCCG

Downstream 100 bases:

>100_bases
CCTCGCCGCCGGGGCTGCACGGTCCTTACCCTCCAGCATCATGACGCTCGCCCTCGTCGTCGCCGTGCTCACCGCCGCAA
CCCCCGCAGGCGGGAAGGCC

Product: diacylglycerol kinase catalytic subunit

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKPFLIVNPASASGRTGRHFDRIARAVRASIGDFECAFTRARGDGVRLAREALASGGKLVVAVGGDGTASEVIDGLTGET
GPRDPEAHFGFIPRGTGGDLCRTLGIDPDVDSAARTLASRDVAVLDLGRLELAGPDGAPVTRHFANVAGFGVSGEVSALV
NRGLKLPSGKLSYMLASARALMSWSDQPVRWRVDGGAWKEERITAVSVCNGRYFGGGMKVAPDARMDDGLFDVVLWKAFG
MGDFIAKRPMLYDGTHVRLENTRVLRARTLEAEPLEGARVRIDSDGESPGRLPARFTILPGALRIRVGR

Sequences:

>Translated_309_residues
MKPFLIVNPASASGRTGRHFDRIARAVRASIGDFECAFTRARGDGVRLAREALASGGKLVVAVGGDGTASEVIDGLTGET
GPRDPEAHFGFIPRGTGGDLCRTLGIDPDVDSAARTLASRDVAVLDLGRLELAGPDGAPVTRHFANVAGFGVSGEVSALV
NRGLKLPSGKLSYMLASARALMSWSDQPVRWRVDGGAWKEERITAVSVCNGRYFGGGMKVAPDARMDDGLFDVVLWKAFG
MGDFIAKRPMLYDGTHVRLENTRVLRARTLEAEPLEGARVRIDSDGESPGRLPARFTILPGALRIRVGR
>Mature_309_residues
MKPFLIVNPASASGRTGRHFDRIARAVRASIGDFECAFTRARGDGVRLAREALASGGKLVVAVGGDGTASEVIDGLTGET
GPRDPEAHFGFIPRGTGGDLCRTLGIDPDVDSAARTLASRDVAVLDLGRLELAGPDGAPVTRHFANVAGFGVSGEVSALV
NRGLKLPSGKLSYMLASARALMSWSDQPVRWRVDGGAWKEERITAVSVCNGRYFGGGMKVAPDARMDDGLFDVVLWKAFG
MGDFIAKRPMLYDGTHVRLENTRVLRARTLEAEPLEGARVRIDSDGESPGRLPARFTILPGALRIRVGR

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=269, Percent_Identity=25.2788104089219, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 32926; Mature: 32926

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFLIVNPASASGRTGRHFDRIARAVRASIGDFECAFTRARGDGVRLAREALASGGKLV
CCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCEEE
VAVGGDGTASEVIDGLTGETGPRDPEAHFGFIPRGTGGDLCRTLGIDPDVDSAARTLASR
EEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHC
DVAVLDLGRLELAGPDGAPVTRHFANVAGFGVSGEVSALVNRGLKLPSGKLSYMLASARA
CEEEEEECCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHH
LMSWSDQPVRWRVDGGAWKEERITAVSVCNGRYFGGGMKVAPDARMDDGLFDVVLWKAFG
HHCCCCCCEEEEECCCCCCCCCEEEEEEECCEEECCCEEECCCCCCCCCHHHHHHHHHCC
MGDFIAKRPMLYDGTHVRLENTRVLRARTLEAEPLEGARVRIDSDGESPGRLPARFTILP
CCHHHHCCCEEECCCEEEECCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCEEEEEEC
GALRIRVGR
CEEEEEECC
>Mature Secondary Structure
MKPFLIVNPASASGRTGRHFDRIARAVRASIGDFECAFTRARGDGVRLAREALASGGKLV
CCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCEEE
VAVGGDGTASEVIDGLTGETGPRDPEAHFGFIPRGTGGDLCRTLGIDPDVDSAARTLASR
EEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHC
DVAVLDLGRLELAGPDGAPVTRHFANVAGFGVSGEVSALVNRGLKLPSGKLSYMLASARA
CEEEEEECCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHH
LMSWSDQPVRWRVDGGAWKEERITAVSVCNGRYFGGGMKVAPDARMDDGLFDVVLWKAFG
HHCCCCCCEEEEECCCCCCCCCEEEEEEECCEEECCCEEECCCCCCCCCHHHHHHHHHCC
MGDFIAKRPMLYDGTHVRLENTRVLRARTLEAEPLEGARVRIDSDGESPGRLPARFTILP
CCHHHHCCCEEECCCEEEECCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCEEEEEEC
GALRIRVGR
CEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]