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The map label for this gene is nusA [H]

Identifier: 220916334

GI number: 220916334

Start: 1378177

End: 1379850

Strand: Direct

Name: nusA [H]

Synonym: A2cp1_1228

Alternate gene names: 220916334

Gene position: 1378177-1379850 (Clockwise)

Preceding gene: 220916333

Following gene: 220916335

Centisome position: 27.4

GC content: 69.47

Gene sequence:

>1674_bases
ATGCAGCAGAACGTCAACCTGAACCTCATCCTCGACCAGGTCGCCAAGGACAAGGGGATCGATCGCGCCAAGCTGATCGA
GATCCTCGAGGAGGCCATCGGCAGCGCCGCCAAGCGCCACTTCGGCCTGGAGCGCAACCTCAAGGCCCGCTACGACGAGG
AGAAGGGGCAGGTGGATCTGTTCCAGGTCCTCACCATCGTGCCCGATCCCACCGAGGAGGCGCCGCTCGCCGATCCGGTG
AACATGGTCCCGGTCGCGGTGGCGCACGAGAAGGGGATCGACGTGGAGCCGGGCGACGAGCTCGACTTCCCCATCTACTA
CCGCGTCGAGGACGAGGCCGAGGCCCGCGCGCAGGACGAGCAGTGGGGCGACCTGCTCAAGCTGAAGACGTACCGCCGGT
CGTTCGGCCGCATCGCCGCGCAGACCGCCAAGCAGGTGATGATCCAGGGCACCCGCAACGCGGAGCGCGAGAACGTCTTC
AACGAGTACAAGGACCGCAAGGGCGAGGTCATCACCGGCATCGTCCGCCGCTTCGAGCGCGGCAACGTCATCGTGGACCT
GGGCCGCGCCGAGGCGGTGCTGCCGGTGCGCGAGCAGGTGCCGCGCGAGAGCTACCGCGCGGGCGACCGCATCCAGGCGT
TCGTGATGGACGTGCTGCGCGAGTCGAAGGGGCCGCAGATCATCCTGTCCCGCGCCTCGGTGGAGCTGCTGAAGAAGCTG
TTCGAGATGGAGGTCCCGGAGATCGCCGAGGGCGTGGTGGTGATCGAGGCCGCCGCCCGCGAGCCGGGCGGCCGCGCCAA
GATCGCGGTCAGCTCGCGCGACTCCGACGTGGATCCGGTGGGCGCGTGCGTGGGCATGAAGGGCAGCCGCGTCCAGGCGG
TGGTCCAGGAGCTCCGCGGCGAGAAGATCGACATCGTCCCCTGGGACGAGGATCCGGCCCGCTTCGTGTGCAACGCCCTG
GCGCCGGCCGAAGTCTCCCGCGTGCTGCTCGACGAGCAGAACCGCGCCATGGAGATCATCGTCCCCGACGACCAGCTCTC
GCTCGCGATCGGCCGCCGCGGCCAGAACGTGCGGCTCGCGTCGCAGGTGACCGGCTGGAAGCTCGACATCAACTCCGAGT
CGCGCGTGAAGGAGATGCGCGAGTTCGCGACGGAGAGCTTCGGCGCGATCGGCATCCCCGAGCAGACCCAGGAGATGCTG
TACGCGCACGGGTTCCGGAAGGCGCAGGACGTGGCCAACGCGGCGCCCGAGATGCTCACGCAGTTCCCCGGCTTCACCAT
GGACATGATCCCCGACCTGCAGAAGCGGGCGCGGGAGCAGTCCATCGAGGACGCCGAGAAGGAGATGCGGCTCGAGCAGG
AGCGCGAGGCCGCCCGCATCGCCGAGGCGCGGCGCCACCCCGACTCGCTCACGCAGGAGGAGCGCTTCGCGCGCGTCCGC
GGGGTGGGCGAGAAGACCATCGAGCAGATGAAGCTGGCCGGCTACCCCACGGTCGAGACGGTGCACAACGAGGCGGACGT
GAACCGCTTCGCCGACTCCACCGGGCTCGGCATCAAGAAGGCCCGCCAGATCAAGCACGCCGTGGGCGTGTACCTGGAGG
AGGAGGCCAAGCTCCGCGCCGAGCTCGACGCGGAGAAGGCCCGGCAGTCCGGCGCGGAGGGCGCGGGCGCTTGA

Upstream 100 bases:

>100_bases
CGACGTGGACGGCGTGCTGCACCGCGTCCCGCACGACCAGATCGCGAAGGCCAACCTCGAGTACGACGTCGAGGGCGACC
TCCGAAGGAAGGACTGATCC

Downstream 100 bases:

>100_bases
CCGAGCCCGTCCGCACCTGCATCGGCTGCGGCGCGAGGGCGCCCCAGCGCGAGCTGGTGCGGCTCAAGGTGATGGGAGAT
CGCGTGACGGTGGATCGCGA

Product: NusA antitermination factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MQQNVNLNLILDQVAKDKGIDRAKLIEILEEAIGSAAKRHFGLERNLKARYDEEKGQVDLFQVLTIVPDPTEEAPLADPV
NMVPVAVAHEKGIDVEPGDELDFPIYYRVEDEAEARAQDEQWGDLLKLKTYRRSFGRIAAQTAKQVMIQGTRNAERENVF
NEYKDRKGEVITGIVRRFERGNVIVDLGRAEAVLPVREQVPRESYRAGDRIQAFVMDVLRESKGPQIILSRASVELLKKL
FEMEVPEIAEGVVVIEAAAREPGGRAKIAVSSRDSDVDPVGACVGMKGSRVQAVVQELRGEKIDIVPWDEDPARFVCNAL
APAEVSRVLLDEQNRAMEIIVPDDQLSLAIGRRGQNVRLASQVTGWKLDINSESRVKEMREFATESFGAIGIPEQTQEML
YAHGFRKAQDVANAAPEMLTQFPGFTMDMIPDLQKRAREQSIEDAEKEMRLEQEREAARIAEARRHPDSLTQEERFARVR
GVGEKTIEQMKLAGYPTVETVHNEADVNRFADSTGLGIKKARQIKHAVGVYLEEEAKLRAELDAEKARQSGAEGAGA

Sequences:

>Translated_557_residues
MQQNVNLNLILDQVAKDKGIDRAKLIEILEEAIGSAAKRHFGLERNLKARYDEEKGQVDLFQVLTIVPDPTEEAPLADPV
NMVPVAVAHEKGIDVEPGDELDFPIYYRVEDEAEARAQDEQWGDLLKLKTYRRSFGRIAAQTAKQVMIQGTRNAERENVF
NEYKDRKGEVITGIVRRFERGNVIVDLGRAEAVLPVREQVPRESYRAGDRIQAFVMDVLRESKGPQIILSRASVELLKKL
FEMEVPEIAEGVVVIEAAAREPGGRAKIAVSSRDSDVDPVGACVGMKGSRVQAVVQELRGEKIDIVPWDEDPARFVCNAL
APAEVSRVLLDEQNRAMEIIVPDDQLSLAIGRRGQNVRLASQVTGWKLDINSESRVKEMREFATESFGAIGIPEQTQEML
YAHGFRKAQDVANAAPEMLTQFPGFTMDMIPDLQKRAREQSIEDAEKEMRLEQEREAARIAEARRHPDSLTQEERFARVR
GVGEKTIEQMKLAGYPTVETVHNEADVNRFADSTGLGIKKARQIKHAVGVYLEEEAKLRAELDAEKARQSGAEGAGA
>Mature_557_residues
MQQNVNLNLILDQVAKDKGIDRAKLIEILEEAIGSAAKRHFGLERNLKARYDEEKGQVDLFQVLTIVPDPTEEAPLADPV
NMVPVAVAHEKGIDVEPGDELDFPIYYRVEDEAEARAQDEQWGDLLKLKTYRRSFGRIAAQTAKQVMIQGTRNAERENVF
NEYKDRKGEVITGIVRRFERGNVIVDLGRAEAVLPVREQVPRESYRAGDRIQAFVMDVLRESKGPQIILSRASVELLKKL
FEMEVPEIAEGVVVIEAAAREPGGRAKIAVSSRDSDVDPVGACVGMKGSRVQAVVQELRGEKIDIVPWDEDPARFVCNAL
APAEVSRVLLDEQNRAMEIIVPDDQLSLAIGRRGQNVRLASQVTGWKLDINSESRVKEMREFATESFGAIGIPEQTQEML
YAHGFRKAQDVANAAPEMLTQFPGFTMDMIPDLQKRAREQSIEDAEKEMRLEQEREAARIAEARRHPDSLTQEERFARVR
GVGEKTIEQMKLAGYPTVETVHNEADVNRFADSTGLGIKKARQIKHAVGVYLEEEAKLRAELDAEKARQSGAEGAGA

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=435, Percent_Identity=37.2413793103448, Blast_Score=283, Evalue=2e-77,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 62225; Mature: 62225

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQNVNLNLILDQVAKDKGIDRAKLIEILEEAIGSAAKRHFGLERNLKARYDEEKGQVDL
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEH
FQVLTIVPDPTEEAPLADPVNMVPVAVAHEKGIDVEPGDELDFPIYYRVEDEAEARAQDE
HHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCHHHHHHCHH
QWGDLLKLKTYRRSFGRIAAQTAKQVMIQGTRNAERENVFNEYKDRKGEVITGIVRRFER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHC
GNVIVDLGRAEAVLPVREQVPRESYRAGDRIQAFVMDVLRESKGPQIILSRASVELLKKL
CCEEEEECCCCCCCCHHHHCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
FEMEVPEIAEGVVVIEAAAREPGGRAKIAVSSRDSDVDPVGACVGMKGSRVQAVVQELRG
HHCCCHHHHCCEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHCC
EKIDIVPWDEDPARFVCNALAPAEVSRVLLDEQNRAMEIIVPDDQLSLAIGRRGQNVRLA
CEEEEECCCCCHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCEEEE
SQVTGWKLDINSESRVKEMREFATESFGAIGIPEQTQEMLYAHGFRKAQDVANAAPEMLT
ECCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
QFPGFTMDMIPDLQKRAREQSIEDAEKEMRLEQEREAARIAEARRHPDSLTQEERFARVR
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
GVGEKTIEQMKLAGYPTVETVHNEADVNRFADSTGLGIKKARQIKHAVGVYLEEEAKLRA
CCCHHHHHHHHHCCCCCHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
ELDAEKARQSGAEGAGA
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQQNVNLNLILDQVAKDKGIDRAKLIEILEEAIGSAAKRHFGLERNLKARYDEEKGQVDL
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEH
FQVLTIVPDPTEEAPLADPVNMVPVAVAHEKGIDVEPGDELDFPIYYRVEDEAEARAQDE
HHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCHHHHHHCHH
QWGDLLKLKTYRRSFGRIAAQTAKQVMIQGTRNAERENVFNEYKDRKGEVITGIVRRFER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHC
GNVIVDLGRAEAVLPVREQVPRESYRAGDRIQAFVMDVLRESKGPQIILSRASVELLKKL
CCEEEEECCCCCCCCHHHHCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
FEMEVPEIAEGVVVIEAAAREPGGRAKIAVSSRDSDVDPVGACVGMKGSRVQAVVQELRG
HHCCCHHHHCCEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHCC
EKIDIVPWDEDPARFVCNALAPAEVSRVLLDEQNRAMEIIVPDDQLSLAIGRRGQNVRLA
CEEEEECCCCCHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCEEEE
SQVTGWKLDINSESRVKEMREFATESFGAIGIPEQTQEMLYAHGFRKAQDVANAAPEMLT
ECCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
QFPGFTMDMIPDLQKRAREQSIEDAEKEMRLEQEREAARIAEARRHPDSLTQEERFARVR
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
GVGEKTIEQMKLAGYPTVETVHNEADVNRFADSTGLGIKKARQIKHAVGVYLEEEAKLRA
CCCHHHHHHHHHCCCCCHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
ELDAEKARQSGAEGAGA
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]