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The map label for this gene is sigB [H]

Identifier: 220916304

GI number: 220916304

Start: 1342177

End: 1343079

Strand: Direct

Name: sigB [H]

Synonym: A2cp1_1197

Alternate gene names: 220916304

Gene position: 1342177-1343079 (Clockwise)

Preceding gene: 220916302

Following gene: 220916310

Centisome position: 26.69

GC content: 70.76

Gene sequence:

>903_bases
ATGAACCGACCGAGCACCGCGAGCAGCACCCTCGACCAGTACCTCCGCGAGATCAACCGGGTCGCGCTCCTCACCGTCGA
GGAGGAGCGGCGGCTCGCCCGCCAGTTCCGCGACGAGGGGAACACGCGCGCCGGCCACCGGCTGGTCGAGGCGAACCTCC
GCTTCGTGGTGAAGGTCGCGTTCGAGTACCGGTCCTACGGGCTGCGCATGGCGGACCTCATCCAGGAAGGCAACATCGGC
CTGATGAAGGCGGTGCAGAAGTTCGATCCGGACAAGGAGATCCGGCTCATCTCCTACGCGGTGTGGTGGATCCGGGCCTA
CATCCAGAACCACATCCTCAAGTCGTGGTCGCTCGTGAAGATCGGCACGACGCAGGCGCAGCGTAAGCTCTTCTTCTCGC
TGGCCCGCACCCGGCACGAGATCGAGCGGCTCACCCCCGGCGCCGGCCTCGCCGAGGAGGGCATCGACGTCGCGCTCGTC
GCCAAGAAGCTCCGCGTGCGGCCGAGCGACGTGGTCGAGATGACCCAGCGCATGGAGGGGCGCGACCTGTCGCTCGACGC
CCCGGTGGCCGACGGGACCAGCACGCACCTCGAGTTCACGCCCGCCGACGGCGAGCCGCAGGACGAGGAGCTGGCCCGCG
CCGAGGAGGACGCGCTGCTCGCCCGTCGGGTGTCCGAGGCGATGGGGCGGCTCGACCCGCGCGAGCGCCACATCGTCGAG
GCGCGGATCATGGGCGAGGGCAAGGAGACGCTCCGCGACCTCGGCCATCACTTCGGCTTCTCGCGCGAGCGCGCCCGCCA
GCTCGAGATCCGCGCGCTCGAGAAGCTGCGCCGCGACCTCGAGCCGGTGGCCGACGAGATCGGCTGGCCGGTGACCGGCG
CCGAGGCGAGCGCGGCCGACTGA

Upstream 100 bases:

>100_bases
CGGCGGCGCCGCTGGCGCGTGATCGGCGGCGATCCGGGGGTGGGCACGGACGTTGCTGGGGCGGGACGGGGCCTACGACG
TTCCCCACCCTGGAGGAGAG

Downstream 100 bases:

>100_bases
GCGCCGCGTCTCCGCGCGGCATCCGCCCGCCCGTCGAGGCCTCGGGGCGAGCGGATGCCCGGAGCGGATGCCGCTAGCCC
CGCTTCACCGCGAACGGCGA

Product: RNA polymerase, sigma 70 family subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MNRPSTASSTLDQYLREINRVALLTVEEERRLARQFRDEGNTRAGHRLVEANLRFVVKVAFEYRSYGLRMADLIQEGNIG
LMKAVQKFDPDKEIRLISYAVWWIRAYIQNHILKSWSLVKIGTTQAQRKLFFSLARTRHEIERLTPGAGLAEEGIDVALV
AKKLRVRPSDVVEMTQRMEGRDLSLDAPVADGTSTHLEFTPADGEPQDEELARAEEDALLARRVSEAMGRLDPRERHIVE
ARIMGEGKETLRDLGHHFGFSRERARQLEIRALEKLRRDLEPVADEIGWPVTGAEASAAD

Sequences:

>Translated_300_residues
MNRPSTASSTLDQYLREINRVALLTVEEERRLARQFRDEGNTRAGHRLVEANLRFVVKVAFEYRSYGLRMADLIQEGNIG
LMKAVQKFDPDKEIRLISYAVWWIRAYIQNHILKSWSLVKIGTTQAQRKLFFSLARTRHEIERLTPGAGLAEEGIDVALV
AKKLRVRPSDVVEMTQRMEGRDLSLDAPVADGTSTHLEFTPADGEPQDEELARAEEDALLARRVSEAMGRLDPRERHIVE
ARIMGEGKETLRDLGHHFGFSRERARQLEIRALEKLRRDLEPVADEIGWPVTGAEASAAD
>Mature_300_residues
MNRPSTASSTLDQYLREINRVALLTVEEERRLARQFRDEGNTRAGHRLVEANLRFVVKVAFEYRSYGLRMADLIQEGNIG
LMKAVQKFDPDKEIRLISYAVWWIRAYIQNHILKSWSLVKIGTTQAQRKLFFSLARTRHEIERLTPGAGLAEEGIDVALV
AKKLRVRPSDVVEMTQRMEGRDLSLDAPVADGTSTHLEFTPADGEPQDEELARAEEDALLARRVSEAMGRLDPRERHIVE
ARIMGEGKETLRDLGHHFGFSRERARQLEIRALEKLRRDLEPVADEIGWPVTGAEASAAD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=285, Percent_Identity=41.0526315789474, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789098, Length=270, Percent_Identity=32.962962962963, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789448, Length=279, Percent_Identity=31.1827956989247, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34150; Mature: 34150

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRPSTASSTLDQYLREINRVALLTVEEERRLARQFRDEGNTRAGHRLVEANLRFVVKVA
CCCCCHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
FEYRSYGLRMADLIQEGNIGLMKAVQKFDPDKEIRLISYAVWWIRAYIQNHILKSWSLVK
HHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
IGTTQAQRKLFFSLARTRHEIERLTPGAGLAEEGIDVALVAKKLRVRPSDVVEMTQRMEG
ECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHCC
RDLSLDAPVADGTSTHLEFTPADGEPQDEELARAEEDALLARRVSEAMGRLDPRERHIVE
CCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ARIMGEGKETLRDLGHHFGFSRERARQLEIRALEKLRRDLEPVADEIGWPVTGAEASAAD
HHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRPSTASSTLDQYLREINRVALLTVEEERRLARQFRDEGNTRAGHRLVEANLRFVVKVA
CCCCCHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
FEYRSYGLRMADLIQEGNIGLMKAVQKFDPDKEIRLISYAVWWIRAYIQNHILKSWSLVK
HHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
IGTTQAQRKLFFSLARTRHEIERLTPGAGLAEEGIDVALVAKKLRVRPSDVVEMTQRMEG
ECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHCC
RDLSLDAPVADGTSTHLEFTPADGEPQDEELARAEEDALLARRVSEAMGRLDPRERHIVE
CCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ARIMGEGKETLRDLGHHFGFSRERARQLEIRALEKLRRDLEPVADEIGWPVTGAEASAAD
HHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1475207 [H]