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The map label for this gene is tyrS

Identifier: 220916278

GI number: 220916278

Start: 1309308

End: 1310570

Strand: Direct

Name: tyrS

Synonym: A2cp1_1171

Alternate gene names: 220916278

Gene position: 1309308-1310570 (Clockwise)

Preceding gene: 220916277

Following gene: 220916281

Centisome position: 26.03

GC content: 71.34

Gene sequence:

>1263_bases
ATGCAGAACCTCCTCGAAGCCCTCGTCTCGCGCAGCCTCGTCCACGACCAGACCCCGGGCCTCCAGGCCCGCCTCGCCCA
GGGCCCCATCACCGGCTACGTCGGGTTCGACCCGACCGCCGACTCGCTGCACGTGGGCCACCTGCTCGCGGTCATGTCGC
TCGCCTGGCTGCAGCGCTGCGGCGGCACGCCCATCATCGTGGTGGGCGGCGGCACGGGCATGGTCGGGGATCCGAGCGGC
AAGCGCTCGGAGCGCCCGGTGCTCTCGGTGGAGGAGATCGACCGGAACGTCGCCGCCATCCGCGCGCAGCTCGAGCGGTT
CGTGTCGTTCGAGGGCCAGAACGCGGCGCGGGTGCGCAACAACGCCGACTGGCTCCGCGCGATCGGCCTGATGGAGTTCC
TCCGCGACGTCGGCAAGCACTTCACCGTCAACTACATGCTGGCGAAGGACTCGGTGAAGGGCCGCATGGAGAGCGGCATC
TCCTTCACCGAGTTCTCGTACCAGCTCATCCAGGCCTACGACTTCTGGCACCTGTTCCGCTCCGAGAAGTGCGAGCTGCA
GATGGGCGGCAGCGACCAGTGGGGCAACATCACCGCCGGCGCCGAGCTGGTCTCGCGCAAGGACGGCGCGAGCGTCCACG
GGCTCACCTTCCCGCTGCTCACCACCGCCTCGGGCACCAAGTTCGGCAAGACCGAGGGCGGCGCGGTGTGGCTCGACCCG
GCGCGGACCTCGCCCTACAAGTTCTTCCAGTTCTGGCTCAACACCGACGACCGCGACGTGGAGCGGCTGCTGAAGTTCTT
CACGTTCCTCTCGGTGGAGGAGATCGCCGCGCTGCTCGCCGAGCAGGCGCGCGACCCGGGGAAGCGCCCGGCGCAGCGCA
GGCTCGCGGAGGACGTCACCGCCCGCGTGCACGGGCCGGACGTCACTCGCAGCGTCGTCGAGGCGAGCCGCATCCTGTTC
GGCGGCACCGACCTGCGCGCGGCGAGCGCGGACGTGCTCGAGGTCCTCGCCGGCGAGATCCCGTCCGCGACGGTGACCGG
CGACGAGCTCGCGGCGCTCACGGTCGCCGACCTCCTCGTCAAGGTCGGGCTGGCCGCGTCGAAGGGCGAGGTCCGCCGCG
GCGTGGCGGGGCGCGGGTTCTCGCTGAACGGGGCGGTGCTCGAGTCCGGCGACGCGAAGGTCGCCGCGGGCGACCTGCTC
GCCGGCGGCTACGCGCTCCTGCAGAAGGGCAAGCGGAACTACGCGCTCGTGAAGGTGCGGTAG

Upstream 100 bases:

>100_bases
CACCGCGAGGAAACCCGCACCGAGGAAGCCGGCCAGGCGAGCCTGACGGCGCGGTTCGGCGTCCAAATCTCGGCCGGGAT
CCGCTAGGCTGCCCGGCGCC

Downstream 100 bases:

>100_bases
GGGGCGCGCCGCGAGGCCGCGGTCACTCCTCGAGCTTCTCCTGCACGCGCGCGATCGACCGCGCCCGGCCGGTCGCGTCG
TCGAGGTCCACGATCGCGCC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MQNLLEALVSRSLVHDQTPGLQARLAQGPITGYVGFDPTADSLHVGHLLAVMSLAWLQRCGGTPIIVVGGGTGMVGDPSG
KRSERPVLSVEEIDRNVAAIRAQLERFVSFEGQNAARVRNNADWLRAIGLMEFLRDVGKHFTVNYMLAKDSVKGRMESGI
SFTEFSYQLIQAYDFWHLFRSEKCELQMGGSDQWGNITAGAELVSRKDGASVHGLTFPLLTTASGTKFGKTEGGAVWLDP
ARTSPYKFFQFWLNTDDRDVERLLKFFTFLSVEEIAALLAEQARDPGKRPAQRRLAEDVTARVHGPDVTRSVVEASRILF
GGTDLRAASADVLEVLAGEIPSATVTGDELAALTVADLLVKVGLAASKGEVRRGVAGRGFSLNGAVLESGDAKVAAGDLL
AGGYALLQKGKRNYALVKVR

Sequences:

>Translated_420_residues
MQNLLEALVSRSLVHDQTPGLQARLAQGPITGYVGFDPTADSLHVGHLLAVMSLAWLQRCGGTPIIVVGGGTGMVGDPSG
KRSERPVLSVEEIDRNVAAIRAQLERFVSFEGQNAARVRNNADWLRAIGLMEFLRDVGKHFTVNYMLAKDSVKGRMESGI
SFTEFSYQLIQAYDFWHLFRSEKCELQMGGSDQWGNITAGAELVSRKDGASVHGLTFPLLTTASGTKFGKTEGGAVWLDP
ARTSPYKFFQFWLNTDDRDVERLLKFFTFLSVEEIAALLAEQARDPGKRPAQRRLAEDVTARVHGPDVTRSVVEASRILF
GGTDLRAASADVLEVLAGEIPSATVTGDELAALTVADLLVKVGLAASKGEVRRGVAGRGFSLNGAVLESGDAKVAAGDLL
AGGYALLQKGKRNYALVKVR
>Mature_420_residues
MQNLLEALVSRSLVHDQTPGLQARLAQGPITGYVGFDPTADSLHVGHLLAVMSLAWLQRCGGTPIIVVGGGTGMVGDPSG
KRSERPVLSVEEIDRNVAAIRAQLERFVSFEGQNAARVRNNADWLRAIGLMEFLRDVGKHFTVNYMLAKDSVKGRMESGI
SFTEFSYQLIQAYDFWHLFRSEKCELQMGGSDQWGNITAGAELVSRKDGASVHGLTFPLLTTASGTKFGKTEGGAVWLDP
ARTSPYKFFQFWLNTDDRDVERLLKFFTFLSVEEIAALLAEQARDPGKRPAQRRLAEDVTARVHGPDVTRSVVEASRILF
GGTDLRAASADVLEVLAGEIPSATVTGDELAALTVADLLVKVGLAASKGEVRRGVAGRGFSLNGAVLESGDAKVAAGDLL
AGGYALLQKGKRNYALVKVR

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=440, Percent_Identity=37.0454545454545, Blast_Score=253, Evalue=3e-67,
Organism=Escherichia coli, GI1787925, Length=422, Percent_Identity=46.9194312796209, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25145802, Length=401, Percent_Identity=35.1620947630923, Blast_Score=224, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI25145805, Length=306, Percent_Identity=34.3137254901961, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6325160, Length=357, Percent_Identity=39.2156862745098, Blast_Score=256, Evalue=6e-69,
Organism=Drosophila melanogaster, GI20130383, Length=440, Percent_Identity=38.8636363636364, Blast_Score=300, Evalue=1e-81,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_ANAD2 (B8JFS8)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002491582.1
- ProteinModelPortal:   B8JFS8
- SMR:   B8JFS8
- GeneID:   7299036
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_1171
- HOGENOM:   HBG288125
- ProtClustDB:   PRK05912
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 45319; Mature: 45319

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 33-33 BINDING 167-167 BINDING 171-171 BINDING 230-230

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNLLEALVSRSLVHDQTPGLQARLAQGPITGYVGFDPTADSLHVGHLLAVMSLAWLQRC
CHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHC
GGTPIIVVGGGTGMVGDPSGKRSERPVLSVEEIDRNVAAIRAQLERFVSFEGQNAARVRN
CCCEEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC
NADWLRAIGLMEFLRDVGKHFTVNYMLAKDSVKGRMESGISFTEFSYQLIQAYDFWHLFR
CHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SEKCELQMGGSDQWGNITAGAELVSRKDGASVHGLTFPLLTTASGTKFGKTEGGAVWLDP
CCCCEEEECCCCCCCCEEHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECC
ARTSPYKFFQFWLNTDDRDVERLLKFFTFLSVEEIAALLAEQARDPGKRPAQRRLAEDVT
CCCCCCEEHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ARVHGPDVTRSVVEASRILFGGTDLRAASADVLEVLAGEIPSATVTGDELAALTVADLLV
HHCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHH
KVGLAASKGEVRRGVAGRGFSLNGAVLESGDAKVAAGDLLAGGYALLQKGKRNYALVKVR
HHCCCCCCCHHHCCCCCCCCCCCCCEEECCCCEEECCHHHHHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure
MQNLLEALVSRSLVHDQTPGLQARLAQGPITGYVGFDPTADSLHVGHLLAVMSLAWLQRC
CHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHC
GGTPIIVVGGGTGMVGDPSGKRSERPVLSVEEIDRNVAAIRAQLERFVSFEGQNAARVRN
CCCEEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC
NADWLRAIGLMEFLRDVGKHFTVNYMLAKDSVKGRMESGISFTEFSYQLIQAYDFWHLFR
CHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SEKCELQMGGSDQWGNITAGAELVSRKDGASVHGLTFPLLTTASGTKFGKTEGGAVWLDP
CCCCEEEECCCCCCCCEEHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECC
ARTSPYKFFQFWLNTDDRDVERLLKFFTFLSVEEIAALLAEQARDPGKRPAQRRLAEDVT
CCCCCCEEHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ARVHGPDVTRSVVEASRILFGGTDLRAASADVLEVLAGEIPSATVTGDELAALTVADLLV
HHCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHH
KVGLAASKGEVRRGVAGRGFSLNGAVLESGDAKVAAGDLLAGGYALLQKGKRNYALVKVR
HHCCCCCCCHHHCCCCCCCCCCCCCEEECCCCEEECCHHHHHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA