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The map label for this gene is rlmE [H]

Identifier: 220916277

GI number: 220916277

Start: 1308357

End: 1309253

Strand: Direct

Name: rlmE [H]

Synonym: A2cp1_1170

Alternate gene names: 220916277

Gene position: 1308357-1309253 (Clockwise)

Preceding gene: 220916269

Following gene: 220916278

Centisome position: 26.01

GC content: 74.47

Gene sequence:

>897_bases
ATGGCCAAGCCCTACGACCCGAAGGACTTCTACTACCGCAAGGCGAAGAAGCAGGGCCTGCGCGCTCGCTCGGCCTTCAA
GATCGAGGAGATCCTGCACCGCCACCGCCTGCTCGGGCGGGGCGACGCGGTGCTCGACCTGGGCGCCGCGCCGGGCGGGT
TCCTGCAGATCCTGGCCGACGCGGTGGGCGAGAAGGGCGTCGCGGTGGGCGTGGACCTCGAGCCCATCCGCAACCTGGGC
AAGCGGTGGGTCCGGACCGCGGTGGTGGACCTGCTCGCGCCGGACGCGCTCGACAAGATCCGCCTGCTGCACGAGGGCCG
CTTCCGGCTGGTCACGAGCGACATGGCGCCGAAGACCATCGGCATCAAGGTGACCGACGAGGCGCGCTCGCTCGAGCTGG
TCCGGATGGCGCTGTCGGTCGCGGAGCAGGTGCTCGTGCCGGGCGGCGCGTTCGTCGCGAAGGTGTTCATGGGCGGCGAT
TTCCCGGCGTTGAAGCGCGAGCTGCAGGGGCGCTTCGCGGCGATGCACGTGATCCGGCCGGAGGCGGTGCGCGAGTCGAG
CTACGAGGTCTACGTGCTCGGCACGGGCTTCCGCGGCGGCGCGGCGGTCGGGCGGGTCGCGCCGCCGGCCGAGGAGCCCG
GGGCGCCCGCAGGGCCGGAGCGCGCGGCGCCCGAGCCGCCGGCCGCGCCGGAGCACGCTGCGCCGCAGCCGCCGGCCGCG
CCGGAGCACGCTGCGGCGCCGGCGAAGGCCGCGGGGCCGGCGGAGACCGCGCCGCGGAAGAAGCCTGCCGCTGCGAAGAA
GGCGACCGCTACGAAGAAGCCCGCGTCGAAGAAGCCCGCCGCGAGGAAACCCACCGCGAGGAAACCCGCACCGAGGAAGC
CGGCCAGGCGAGCCTGA

Upstream 100 bases:

>100_bases
CAGAAGGATCTTGATCCTGGACATTGTCGGCTTGACTGCCCTGCGCGAGCCGCAGGATACCGGGCGGCGGAACCCCCGGC
AAACGGACGTCAGCCCACAC

Downstream 100 bases:

>100_bases
CGGCGCGGTTCGGCGTCCAAATCTCGGCCGGGATCCGCTAGGCTGCCCGGCGCCATGCAGAACCTCCTCGAAGCCCTCGT
CTCGCGCAGCCTCGTCCACG

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MAKPYDPKDFYYRKAKKQGLRARSAFKIEEILHRHRLLGRGDAVLDLGAAPGGFLQILADAVGEKGVAVGVDLEPIRNLG
KRWVRTAVVDLLAPDALDKIRLLHEGRFRLVTSDMAPKTIGIKVTDEARSLELVRMALSVAEQVLVPGGAFVAKVFMGGD
FPALKRELQGRFAAMHVIRPEAVRESSYEVYVLGTGFRGGAAVGRVAPPAEEPGAPAGPERAAPEPPAAPEHAAPQPPAA
PEHAAAPAKAAGPAETAPRKKPAAAKKATATKKPASKKPAARKPTARKPAPRKPARRA

Sequences:

>Translated_298_residues
MAKPYDPKDFYYRKAKKQGLRARSAFKIEEILHRHRLLGRGDAVLDLGAAPGGFLQILADAVGEKGVAVGVDLEPIRNLG
KRWVRTAVVDLLAPDALDKIRLLHEGRFRLVTSDMAPKTIGIKVTDEARSLELVRMALSVAEQVLVPGGAFVAKVFMGGD
FPALKRELQGRFAAMHVIRPEAVRESSYEVYVLGTGFRGGAAVGRVAPPAEEPGAPAGPERAAPEPPAAPEHAAPQPPAA
PEHAAAPAKAAGPAETAPRKKPAAAKKATATKKPASKKPAARKPTARKPAPRKPARRA
>Mature_297_residues
AKPYDPKDFYYRKAKKQGLRARSAFKIEEILHRHRLLGRGDAVLDLGAAPGGFLQILADAVGEKGVAVGVDLEPIRNLGK
RWVRTAVVDLLAPDALDKIRLLHEGRFRLVTSDMAPKTIGIKVTDEARSLELVRMALSVAEQVLVPGGAFVAKVFMGGDF
PALKRELQGRFAAMHVIRPEAVRESSYEVYVLGTGFRGGAAVGRVAPPAEEPGAPAGPERAAPEPPAAPEHAAPQPPAAP
EHAAAPAKAAGPAETAPRKKPAAAKKATATKKPASKKPAARKPTARKPAPRKPARRA

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7110661, Length=195, Percent_Identity=35.3846153846154, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI29029591, Length=195, Percent_Identity=35.3846153846154, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI29029589, Length=195, Percent_Identity=35.3846153846154, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI7019377, Length=202, Percent_Identity=36.1386138613861, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI194097365, Length=191, Percent_Identity=35.6020942408377, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI1789569, Length=194, Percent_Identity=37.1134020618557, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17553860, Length=231, Percent_Identity=32.034632034632, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71987561, Length=231, Percent_Identity=32.034632034632, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71987550, Length=231, Percent_Identity=32.034632034632, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17553474, Length=194, Percent_Identity=35.0515463917526, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17554650, Length=197, Percent_Identity=31.4720812182741, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319535, Length=204, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=193, Percent_Identity=32.6424870466321, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18859957, Length=195, Percent_Identity=32.3076923076923, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24647580, Length=207, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648639, Length=233, Percent_Identity=30.4721030042918, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356387, Length=198, Percent_Identity=29.2929292929293, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 31628; Mature: 31497

Theoretical pI: Translated: 10.96; Mature: 10.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPYDPKDFYYRKAKKQGLRARSAFKIEEILHRHRLLGRGDAVLDLGAAPGGFLQILAD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHH
AVGEKGVAVGVDLEPIRNLGKRWVRTAVVDLLAPDALDKIRLLHEGRFRLVTSDMAPKTI
HHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCEE
GIKVTDEARSLELVRMALSVAEQVLVPGGAFVAKVFMGGDFPALKRELQGRFAAMHVIRP
EEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCH
EAVRESSYEVYVLGTGFRGGAAVGRVAPPAEEPGAPAGPERAAPEPPAAPEHAAPQPPAA
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PEHAAAPAKAAGPAETAPRKKPAAAKKATATKKPASKKPAARKPTARKPAPRKPARRA
CCHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKPYDPKDFYYRKAKKQGLRARSAFKIEEILHRHRLLGRGDAVLDLGAAPGGFLQILAD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHH
AVGEKGVAVGVDLEPIRNLGKRWVRTAVVDLLAPDALDKIRLLHEGRFRLVTSDMAPKTI
HHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCEE
GIKVTDEARSLELVRMALSVAEQVLVPGGAFVAKVFMGGDFPALKRELQGRFAAMHVIRP
EEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCH
EAVRESSYEVYVLGTGFRGGAAVGRVAPPAEEPGAPAGPERAAPEPPAAPEHAAPQPPAA
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PEHAAAPAKAAGPAETAPRKKPAAAKKATATKKPASKKPAARKPTARKPAPRKPARRA
CCHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA