Click here to switch to the map view.

The map label for this gene is czcB [H]

Identifier: 220916193

GI number: 220916193

Start: 1212574

End: 1213980

Strand: Reverse

Name: czcB [H]

Synonym: A2cp1_1081

Alternate gene names: 220916193

Gene position: 1213980-1212574 (Counterclockwise)

Preceding gene: 220916194

Following gene: 220916192

Centisome position: 24.14

GC content: 77.47

Gene sequence:

>1407_bases
ATGCGTCCGTTCCCTTCCGCTGGTCCCCTCCTGTCCGCCGTGGTCGCCGCGGCCATCGCGCTCCAGGGCTGCGCGCGCGA
CGCATCGCCGCTCGAGCGCGCGGTCGCCGCGCCCGCCCCCACGACCGCCGCGCCGGCCCCCGCGACCCCGGCACCCGCTG
CCCGCGAGGCGACCGAGGCCTGGTGCGGGGAGCACGCGGTCCCCGAGGCGAAGTGCACGAAGTGCAACCCGCGGCTCATC
GAGCAGTTCATCGCCGCGGGGGACTACTGCCGCGAGCACGGGTTCCCGGAGTCGGCGTGCCCCTACTGTCACCCGGAGCT
GGTGACCGCGGCCGGGCACCCGCTGCCGGCGTTCCCCGCGCCCGGCACGAAGATCCGCCTCGCGGGCCCCGAGACCGAGC
AGGAGGCGGGGATCGAGACGGTGCGCGCCGAGGTGCGCCCCTTCGCGGGCACGGTGGACGTGGTCGGTCAGCTCGAGCTC
GACCAGAACCGCCTGGCCCGCCTCTCCGCGCGCGGTGACGCGACGGTGGTCGAGCTGAAGGTGGACGCCGGCGAGGACGT
CCGCCGGGGACAGCCGCTCGTCGTGCTCGCGTCGGGCGCGGTGGGCGGGGCGCAGTCGAGTCGCGTGGCGGCGGAGGCGC
GCGTCGAGGCGGCCAGGAGCGCGCTCGAGCGCGAGGAGCAGCTCGCGCAGAGCGGGATCAGCCCACGGCAGGACGTGGAA
CGGGCCCGCGCCCAGCTCGCGTCGGCAGAGGCGGAGCGCGACGCCGCCCGCGCCGCGCTTCGCGCCGCCGGCGCCGCCGA
GTCGGGCCGGGGCGGCCGCTATGCGCTGGTCGCGCCGTTCGCCGGCACGGTGATCGCCCGCGACGCCGTCGCCGGGCGGA
GCGTGGGCGAGGACGACGTCCTCCTCGAGATCGCGGACGTCCGGACGCTCTGGGCCGTCCTCGAGGTCCCCGAGGAGCAG
GCGGGGAGCGTCCGGCCCGGGCAGCGCGTGGCCCTGACCGTGGACGGGAGCGGTGAGCGCCGGGAGGCGCGCGTCACCCG
CGTGAGCCCGGCGGTCGACCGCCGGACCCGCACCGTCCGCGTGCGGGTGGACGTCGCGAACGAGGACCGGACGCTGCGCG
CGGGGTCCTTCCTGCGCGCGTCCATCGAGGTGAGCGCGCCGCGCGGCGCGCTGCTCCTCCCGCGCGACGCGGTCCAGCGC
GCGCAGGAGCACGACCTCGTGTTCGTCCGGACGAGGCCGGGCGAGTACGACCCGGTGGAGGTCCGCCTCGGCGTCCGCAC
GCGCGACGAGGTCGAGGTGGTCGCCGGCCTGACGCCCGGGGCCGAGGTGGTGACGGCCGGCGCGTTCGTCCTCAAGACCG
AGATCCTGAAGGACTCGATCGGCGCAGGCTGCGCCGACGGGCACTAG

Upstream 100 bases:

>100_bases
CGCAGGGCCGTCCCTTCCAGCCCCGTGCAGCCGCTCGGTGCGCGCGAAGCGTCGCGCGCGCCACGTCGCCGCGCCCGCGT
GCGCGGCGGGAGAAGCCGTC

Downstream 100 bases:

>100_bases
GGGCGGCCCATGCTGACGAGGATCATCGACCTCTCGCTCCGCCACCGCTGGCTGGTGCTCGCGACCTGGGGCGCGATCGC
GATCGCGGGGCTGCTGTCGT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MRPFPSAGPLLSAVVAAAIALQGCARDASPLERAVAAPAPTTAAPAPATPAPAAREATEAWCGEHAVPEAKCTKCNPRLI
EQFIAAGDYCREHGFPESACPYCHPELVTAAGHPLPAFPAPGTKIRLAGPETEQEAGIETVRAEVRPFAGTVDVVGQLEL
DQNRLARLSARGDATVVELKVDAGEDVRRGQPLVVLASGAVGGAQSSRVAAEARVEAARSALEREEQLAQSGISPRQDVE
RARAQLASAEAERDAARAALRAAGAAESGRGGRYALVAPFAGTVIARDAVAGRSVGEDDVLLEIADVRTLWAVLEVPEEQ
AGSVRPGQRVALTVDGSGERREARVTRVSPAVDRRTRTVRVRVDVANEDRTLRAGSFLRASIEVSAPRGALLLPRDAVQR
AQEHDLVFVRTRPGEYDPVEVRLGVRTRDEVEVVAGLTPGAEVVTAGAFVLKTEILKDSIGAGCADGH

Sequences:

>Translated_468_residues
MRPFPSAGPLLSAVVAAAIALQGCARDASPLERAVAAPAPTTAAPAPATPAPAAREATEAWCGEHAVPEAKCTKCNPRLI
EQFIAAGDYCREHGFPESACPYCHPELVTAAGHPLPAFPAPGTKIRLAGPETEQEAGIETVRAEVRPFAGTVDVVGQLEL
DQNRLARLSARGDATVVELKVDAGEDVRRGQPLVVLASGAVGGAQSSRVAAEARVEAARSALEREEQLAQSGISPRQDVE
RARAQLASAEAERDAARAALRAAGAAESGRGGRYALVAPFAGTVIARDAVAGRSVGEDDVLLEIADVRTLWAVLEVPEEQ
AGSVRPGQRVALTVDGSGERREARVTRVSPAVDRRTRTVRVRVDVANEDRTLRAGSFLRASIEVSAPRGALLLPRDAVQR
AQEHDLVFVRTRPGEYDPVEVRLGVRTRDEVEVVAGLTPGAEVVTAGAFVLKTEILKDSIGAGCADGH
>Mature_468_residues
MRPFPSAGPLLSAVVAAAIALQGCARDASPLERAVAAPAPTTAAPAPATPAPAAREATEAWCGEHAVPEAKCTKCNPRLI
EQFIAAGDYCREHGFPESACPYCHPELVTAAGHPLPAFPAPGTKIRLAGPETEQEAGIETVRAEVRPFAGTVDVVGQLEL
DQNRLARLSARGDATVVELKVDAGEDVRRGQPLVVLASGAVGGAQSSRVAAEARVEAARSALEREEQLAQSGISPRQDVE
RARAQLASAEAERDAARAALRAAGAAESGRGGRYALVAPFAGTVIARDAVAGRSVGEDDVLLEIADVRTLWAVLEVPEEQ
AGSVRPGQRVALTVDGSGERREARVTRVSPAVDRRTRTVRVRVDVANEDRTLRAGSFLRASIEVSAPRGALLLPRDAVQR
AQEHDLVFVRTRPGEYDPVEVRLGVRTRDEVEVVAGLTPGAEVVTAGAFVLKTEILKDSIGAGCADGH

Specific function: CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete czc efflux system (CzcABC). The CzcB protein is thought to funnel zinc cations to the CzcA transport protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786787, Length=329, Percent_Identity=24.3161094224924, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081796, Length=321, Percent_Identity=25.5451713395639, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87082036, Length=291, Percent_Identity=24.0549828178694, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 49077; Mature: 49077

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPFPSAGPLLSAVVAAAIALQGCARDASPLERAVAAPAPTTAAPAPATPAPAAREATEA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
WCGEHAVPEAKCTKCNPRLIEQFIAAGDYCREHGFPESACPYCHPELVTAAGHPLPAFPA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCC
PGTKIRLAGPETEQEAGIETVRAEVRPFAGTVDVVGQLELDQNRLARLSARGDATVVELK
CCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHCCCCCEEEEEE
VDAGEDVRRGQPLVVLASGAVGGAQSSRVAAEARVEAARSALEREEQLAQSGISPRQDVE
ECCCCHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RARAQLASAEAERDAARAALRAAGAAESGRGGRYALVAPFAGTVIARDAVAGRSVGEDDV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCE
LLEIADVRTLWAVLEVPEEQAGSVRPGQRVALTVDGSGERREARVTRVSPAVDRRTRTVR
EEEHHHHHHHHHHHHCCHHHCCCCCCCCEEEEEECCCCCCCHHHEEECCCHHHCCCEEEE
VRVDVANEDRTLRAGSFLRASIEVSAPRGALLLPRDAVQRAQEHDLVFVRTRPGEYDPVE
EEEEECCCCCEEECCCEEEEEEEEECCCCEEECCHHHHHHHHHCCEEEEECCCCCCCCEE
VRLGVRTRDEVEVVAGLTPGAEVVTAGAFVLKTEILKDSIGAGCADGH
EEECCCCCCCEEEEECCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRPFPSAGPLLSAVVAAAIALQGCARDASPLERAVAAPAPTTAAPAPATPAPAAREATEA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
WCGEHAVPEAKCTKCNPRLIEQFIAAGDYCREHGFPESACPYCHPELVTAAGHPLPAFPA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCC
PGTKIRLAGPETEQEAGIETVRAEVRPFAGTVDVVGQLELDQNRLARLSARGDATVVELK
CCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHCCCCCEEEEEE
VDAGEDVRRGQPLVVLASGAVGGAQSSRVAAEARVEAARSALEREEQLAQSGISPRQDVE
ECCCCHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RARAQLASAEAERDAARAALRAAGAAESGRGGRYALVAPFAGTVIARDAVAGRSVGEDDV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCE
LLEIADVRTLWAVLEVPEEQAGSVRPGQRVALTVDGSGERREARVTRVSPAVDRRTRTVR
EEEHHHHHHHHHHHHCCHHHCCCCCCCCEEEEEECCCCCCCHHHEEECCCHHHCCCEEEE
VRVDVANEDRTLRAGSFLRASIEVSAPRGALLLPRDAVQRAQEHDLVFVRTRPGEYDPVE
EEEEECCCCCEEECCCEEEEEEEEECCCCEEECCHHHHHHHHHCCEEEEECCCCCCCCEE
VRLGVRTRDEVEVVAGLTPGAEVVTAGAFVLKTEILKDSIGAGCADGH
EEECCCCCCCEEEEECCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8829543 [H]