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The map label for this gene is ydgK [H]

Identifier: 220916148

GI number: 220916148

Start: 1158528

End: 1159766

Strand: Direct

Name: ydgK [H]

Synonym: A2cp1_1035

Alternate gene names: 220916148

Gene position: 1158528-1159766 (Clockwise)

Preceding gene: 220916147

Following gene: 220916149

Centisome position: 23.04

GC content: 75.46

Gene sequence:

>1239_bases
ATGCCACGCCTCACCCCCGCCACCGCCCGGCACCACGGGCGGCTCGCAGTGCTGCTCGCCGCGCTCTCGGCGCTCGGCCC
GTTCTCCACCGACGCGTACCTCCCGTCGTTCCCGGAGATCGGGCGGGTCTTCGGCGCGCCGGCGGTGCTGGTGCAGCAGA
CGCTCACCGCGTACATGGTGCCGTTCTCGGTGATGACGCTCTGGCAGGGCGCCATCTCCGACGCGCTGGGCCGCCGGCGC
GTGACGCTCGCGATGCTGGCGCTGTTCGCGCTCGCCTCCGTCGGCTGCATGCTCTCCTGGCGCATCGAGGCGCTCGTCGC
GTTCCGGGCGCTGCAGGGCGCGACCGCCGGGGCGGGCATGGTGATCGGCCGCGCGGTGGTGCGCGACCTGCTCGACGGGG
CCGAGGCCCGCCGGCTGATGAGCCGCATCGCGCTGGTGTTCGCGATCGCGCCGGCGCTCGGGCCGGTCGTGGGCGGCTGG
CTGCACGTGTGGCTGGGCTGGCGCGCGGTGTTCGGCTTCCTGGCGCTGTTCTCCGCGGCCCTGTGCGCCTGGTGCTGGCG
CGCGCTGCCCGAGACGCTGGCGCCGGCGCAGCGGCGGCCGCTCCAGGTCCGCTCCATGCTGCGCGGCTACCGCGAGGTGC
TCTCGTCCCGCGCGTTCCTGGCGCTGGTGGCGGGCGTCACCTGCAGCTTCTCGGCGGTCTTCCTGTACATCGTGGCCGCG
CCGGTGTTCCTGCTCCGCCACCTCGGGGTCGGCGAGACCGGCTTCCTCTGGCTGTTCGGCCCCATCAGCGGCGGCATGAT
GCTCGGCAACTGGGCGTCCGGCGCGCTCGCGGGACGGATGACGAACCAGCGCACCGTCGCCTGGGGCTTCGCCGCGATGG
CGGTGGCCGCCCTCGCGAACGTGCTGTTCCACGCGCTGCACGCGCCCGCCCTGCCGTGGAGCGTGCTGCCGCTCGTCGGC
TACGTGCTGGGCACGTCCCTCGCGATGCCCTCGCTGACCCTCATGGCGCTCGACCTGTTCCCGGAACGGCGCGGGATGGC
CTCCTCCTGCCAGGCGTTCGTGCAGACGGCGGGCAACGCCCTGGTGGCGGCGGCGCTCGCGCCGCTGCTGTGGGGCTCGG
CACGGACGCTCTCGCTCGGCATGGCGGGCGGCCTCGCGGTGGGCGCGCTCGCGTTCGCCGCGTACGCCGCGGTCCCGCCG
TCCGAGCCCGCCAGCCGACCCGAGCGCCGCGCGGCCTGA

Upstream 100 bases:

>100_bases
GGCGGCGCATGCGCTCGCTCAACATCGGGTGAGCCCGGGCGCGCCGCCCGGAGGCGGCTTGAGCGCCGCCGCCGGAGGGG
CTACACCTCGGCTCCCCCCG

Downstream 100 bases:

>100_bases
CCGGGCGAGCCCGGCCCGCCCTTCGCCGTGCTATCACCTCTGCCCGTGCGGGCGCTCCGGCGTCCGCGCCGCCCCGCCCG
CGGCACGAGGGGCGGGGAGG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MPRLTPATARHHGRLAVLLAALSALGPFSTDAYLPSFPEIGRVFGAPAVLVQQTLTAYMVPFSVMTLWQGAISDALGRRR
VTLAMLALFALASVGCMLSWRIEALVAFRALQGATAGAGMVIGRAVVRDLLDGAEARRLMSRIALVFAIAPALGPVVGGW
LHVWLGWRAVFGFLALFSAALCAWCWRALPETLAPAQRRPLQVRSMLRGYREVLSSRAFLALVAGVTCSFSAVFLYIVAA
PVFLLRHLGVGETGFLWLFGPISGGMMLGNWASGALAGRMTNQRTVAWGFAAMAVAALANVLFHALHAPALPWSVLPLVG
YVLGTSLAMPSLTLMALDLFPERRGMASSCQAFVQTAGNALVAAALAPLLWGSARTLSLGMAGGLAVGALAFAAYAAVPP
SEPASRPERRAA

Sequences:

>Translated_412_residues
MPRLTPATARHHGRLAVLLAALSALGPFSTDAYLPSFPEIGRVFGAPAVLVQQTLTAYMVPFSVMTLWQGAISDALGRRR
VTLAMLALFALASVGCMLSWRIEALVAFRALQGATAGAGMVIGRAVVRDLLDGAEARRLMSRIALVFAIAPALGPVVGGW
LHVWLGWRAVFGFLALFSAALCAWCWRALPETLAPAQRRPLQVRSMLRGYREVLSSRAFLALVAGVTCSFSAVFLYIVAA
PVFLLRHLGVGETGFLWLFGPISGGMMLGNWASGALAGRMTNQRTVAWGFAAMAVAALANVLFHALHAPALPWSVLPLVG
YVLGTSLAMPSLTLMALDLFPERRGMASSCQAFVQTAGNALVAAALAPLLWGSARTLSLGMAGGLAVGALAFAAYAAVPP
SEPASRPERRAA
>Mature_411_residues
PRLTPATARHHGRLAVLLAALSALGPFSTDAYLPSFPEIGRVFGAPAVLVQQTLTAYMVPFSVMTLWQGAISDALGRRRV
TLAMLALFALASVGCMLSWRIEALVAFRALQGATAGAGMVIGRAVVRDLLDGAEARRLMSRIALVFAIAPALGPVVGGWL
HVWLGWRAVFGFLALFSAALCAWCWRALPETLAPAQRRPLQVRSMLRGYREVLSSRAFLALVAGVTCSFSAVFLYIVAAP
VFLLRHLGVGETGFLWLFGPISGGMMLGNWASGALAGRMTNQRTVAWGFAAMAVAALANVLFHALHAPALPWSVLPLVGY
VLGTSLAMPSLTLMALDLFPERRGMASSCQAFVQTAGNALVAAALAPLLWGSARTLSLGMAGGLAVGALAFAAYAAVPPS
EPASRPERRAA

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=412, Percent_Identity=29.8543689320388, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI48994889, Length=343, Percent_Identity=26.530612244898, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1790146, Length=344, Percent_Identity=28.4883720930233, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790794, Length=316, Percent_Identity=25.3164556962025, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787065, Length=367, Percent_Identity=28.6103542234332, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082312, Length=196, Percent_Identity=29.0816326530612, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319517, Length=208, Percent_Identity=27.4038461538462, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43392; Mature: 43261

Theoretical pI: Translated: 11.80; Mature: 11.80

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRLTPATARHHGRLAVLLAALSALGPFSTDAYLPSFPEIGRVFGAPAVLVQQTLTAYMV
CCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHH
PFSVMTLWQGAISDALGRRRVTLAMLALFALASVGCMLSWRIEALVAFRALQGATAGAGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VIGRAVVRDLLDGAEARRLMSRIALVFAIAPALGPVVGGWLHVWLGWRAVFGFLALFSAA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LCAWCWRALPETLAPAQRRPLQVRSMLRGYREVLSSRAFLALVAGVTCSFSAVFLYIVAA
HHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PVFLLRHLGVGETGFLWLFGPISGGMMLGNWASGALAGRMTNQRTVAWGFAAMAVAALAN
HHHHHHHCCCCCCCCEEEEECCCCCEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHH
VLFHALHAPALPWSVLPLVGYVLGTSLAMPSLTLMALDLFPERRGMASSCQAFVQTAGNA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHCHH
LVAAALAPLLWGSARTLSLGMAGGLAVGALAFAAYAAVPPSEPASRPERRAA
HHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]