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The map label for this gene is fhlA [H]

Identifier: 220916147

GI number: 220916147

Start: 1157110

End: 1158459

Strand: Direct

Name: fhlA [H]

Synonym: A2cp1_1034

Alternate gene names: 220916147

Gene position: 1157110-1158459 (Clockwise)

Preceding gene: 220916140

Following gene: 220916148

Centisome position: 23.01

GC content: 76.3

Gene sequence:

>1350_bases
ATGAGCGCATCGGAGCAGGGCAGCGAGGTCGCCTTCGCCGCGGTGAACGCGGCCTTCGGATCCCTCGGGCGCGTGTGCAT
GGCGCTCGACGACCAGTTCCGGGTGCGGCACGTGTCGTCGCGCCTGGACGTGCTGCTCGGGGAAGGCGCCGCGGCGCGGA
CGGTCGGGACGCCGGTGGAGGCGCTGCTCGGCGCCGAGCTGTTCGGCGCGGACGGCCCCATCCGCCACGCGCTGCTGGCG
GGCGAGAAGCGCGAGGGCTGGCGCGCGCTGCTCCGGGCCGGCGACGGTGCCTCGCACCTGCTCTCCGTCACCGCCGCGCC
GCTCCAGCTCGATCCGCACGGCGTGTGCGCGCGGGACGCGCGCTACCTGGTGGTGCTCCGGCCCGCCGAGGAGGAGGCGG
GCGACGCCGCCGCCGCGCTGGCCACCACCGGCATCATCAGCCGCTCGCGCGCCATGGGCCGGGTGGTGCGGCTGGTGGAG
AGCCTGCAGTACAGCGAGGCCTCGGTGCTCGTCACCGGCGAGAGCGGCTCGGGCAAGGAGGTCCTGGCGCGGCTCATCCA
CGCGCACTCGCCGCGCCGGAGCGGGCCGTTCGTGGCGGTGAACGCCGCCGCGCTGCCGGGCGACCTGCTCGAGAGCGAGC
TGTTCGGCCACGTGCGCGGCGCGTTCACCGGCGCCACCCGCGACCGGCCGGGCCGCTTCGCCGCGGCCGCCCAGGGCACG
CTGTTCCTCGACGAGGTCGGCGACCTGCCGCTGCACCTGCAGGTCAAGTTGCTGCGCGTGCTGCAGGAGCACACCTACGA
GCGCGTCGGCGAGAACCAGCCGCGCCGCGCCGACGCGCGCATCATCGCGGCCACGAACCGCGACCTGCGCCGCGCCGTGG
CGGACGGGACGTTCCGCGAGGACCTGTACTACCGCCTGCGCGTGTTCCCGGTGGAGATCCCCCCGCTCCGTGAGCGGCGG
GAGGACATCGAGCCGATGGCGCAGCTCCTCCTGTCCCGCGTCTGCGCGCGGGCCGGCCGGGCGGTGCGCTTCTCGCCGGA
CGCGCTCCGGGCGCTCATGTCGCACTCGTGGCCGGGCAACGTGCGCGAGCTCGAGAACGCGCTCGAGTTCGCCGTGACGG
TGTGCCGGGGCCAGACGGTGCAGCCCGAGGACCTGCCGCCGGAGGTGCTCGGCGGCGGGGCGCCGGAGCACGCGCCGGTG
CGCGCGTTCGAGCCGGCGGACGCGTACGAGCGCGCCGCCATCGAGGCCGCCCTGCAGGCGCACCGCTGGAGCCGCGCGGA
GGCGGCGCGAGCGCTGGGGCTCTCGCGCAGCACGCTCTGGCGGCGCATGCGCTCGCTCAACATCGGGTGA

Upstream 100 bases:

>100_bases
AGCGGTTGCGACGGGCGCGCCGGTCGCGACGACACGGAACGTTTCAGCGCGACGAAACACCGCGTTTCGCGCTGCGACGC
GACATGCCAGCCTGCGGCAC

Downstream 100 bases:

>100_bases
GCCCGGGCGCGCCGCCCGGAGGCGGCTTGAGCGCCGCCGCCGGAGGGGCTACACCTCGGCTCCCCCCGATGCCACGCCTC
ACCCCCGCCACCGCCCGGCA

Product: Fis family sigma-54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSASEQGSEVAFAAVNAAFGSLGRVCMALDDQFRVRHVSSRLDVLLGEGAAARTVGTPVEALLGAELFGADGPIRHALLA
GEKREGWRALLRAGDGASHLLSVTAAPLQLDPHGVCARDARYLVVLRPAEEEAGDAAAALATTGIISRSRAMGRVVRLVE
SLQYSEASVLVTGESGSGKEVLARLIHAHSPRRSGPFVAVNAAALPGDLLESELFGHVRGAFTGATRDRPGRFAAAAQGT
LFLDEVGDLPLHLQVKLLRVLQEHTYERVGENQPRRADARIIAATNRDLRRAVADGTFREDLYYRLRVFPVEIPPLRERR
EDIEPMAQLLLSRVCARAGRAVRFSPDALRALMSHSWPGNVRELENALEFAVTVCRGQTVQPEDLPPEVLGGGAPEHAPV
RAFEPADAYERAAIEAALQAHRWSRAEAARALGLSRSTLWRRMRSLNIG

Sequences:

>Translated_449_residues
MSASEQGSEVAFAAVNAAFGSLGRVCMALDDQFRVRHVSSRLDVLLGEGAAARTVGTPVEALLGAELFGADGPIRHALLA
GEKREGWRALLRAGDGASHLLSVTAAPLQLDPHGVCARDARYLVVLRPAEEEAGDAAAALATTGIISRSRAMGRVVRLVE
SLQYSEASVLVTGESGSGKEVLARLIHAHSPRRSGPFVAVNAAALPGDLLESELFGHVRGAFTGATRDRPGRFAAAAQGT
LFLDEVGDLPLHLQVKLLRVLQEHTYERVGENQPRRADARIIAATNRDLRRAVADGTFREDLYYRLRVFPVEIPPLRERR
EDIEPMAQLLLSRVCARAGRAVRFSPDALRALMSHSWPGNVRELENALEFAVTVCRGQTVQPEDLPPEVLGGGAPEHAPV
RAFEPADAYERAAIEAALQAHRWSRAEAARALGLSRSTLWRRMRSLNIG
>Mature_448_residues
SASEQGSEVAFAAVNAAFGSLGRVCMALDDQFRVRHVSSRLDVLLGEGAAARTVGTPVEALLGAELFGADGPIRHALLAG
EKREGWRALLRAGDGASHLLSVTAAPLQLDPHGVCARDARYLVVLRPAEEEAGDAAAALATTGIISRSRAMGRVVRLVES
LQYSEASVLVTGESGSGKEVLARLIHAHSPRRSGPFVAVNAAALPGDLLESELFGHVRGAFTGATRDRPGRFAAAAQGTL
FLDEVGDLPLHLQVKLLRVLQEHTYERVGENQPRRADARIIAATNRDLRRAVADGTFREDLYYRLRVFPVEIPPLRERRE
DIEPMAQLLLSRVCARAGRAVRFSPDALRALMSHSWPGNVRELENALEFAVTVCRGQTVQPEDLPPEVLGGGAPEHAPVR
AFEPADAYERAAIEAALQAHRWSRAEAARALGLSRSTLWRRMRSLNIG

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=311, Percent_Identity=49.8392282958199, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1788550, Length=354, Percent_Identity=43.5028248587571, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1790437, Length=301, Percent_Identity=46.5116279069767, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI87082117, Length=326, Percent_Identity=46.6257668711656, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1789233, Length=316, Percent_Identity=43.0379746835443, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI1790299, Length=332, Percent_Identity=43.6746987951807, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1788905, Length=326, Percent_Identity=44.478527607362, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI87082152, Length=330, Percent_Identity=42.7272727272727, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1786524, Length=319, Percent_Identity=40.7523510971787, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI87081872, Length=306, Percent_Identity=37.5816993464052, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1787583, Length=361, Percent_Identity=33.5180055401662, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1789828, Length=232, Percent_Identity=41.8103448275862, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI87081858, Length=283, Percent_Identity=32.8621908127208, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48508; Mature: 48377

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4 ; PS51009 CYTCII

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASEQGSEVAFAAVNAAFGSLGRVCMALDDQFRVRHVSSRLDVLLGEGAAARTVGTPVE
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHEEECCCCCCCCCCCHHH
ALLGAELFGADGPIRHALLAGEKREGWRALLRAGDGASHLLSVTAAPLQLDPHGVCARDA
HHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCCCCC
RYLVVLRPAEEEAGDAAAALATTGIISRSRAMGRVVRLVESLQYSEASVLVTGESGSGKE
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
VLARLIHAHSPRRSGPFVAVNAAALPGDLLESELFGHVRGAFTGATRDRPGRFAAAAQGT
HHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHCCCC
LFLDEVGDLPLHLQVKLLRVLQEHTYERVGENQPRRADARIIAATNRDLRRAVADGTFRE
EEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHCCCHHH
DLYYRLRVFPVEIPPLRERREDIEPMAQLLLSRVCARAGRAVRFSPDALRALMSHSWPGN
HHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCC
VRELENALEFAVTVCRGQTVQPEDLPPEVLGGGAPEHAPVRAFEPADAYERAAIEAALQA
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HRWSRAEAARALGLSRSTLWRRMRSLNIG
HHHHHHHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SASEQGSEVAFAAVNAAFGSLGRVCMALDDQFRVRHVSSRLDVLLGEGAAARTVGTPVE
CCCCCCCCEEHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHEEECCCCCCCCCCCHHH
ALLGAELFGADGPIRHALLAGEKREGWRALLRAGDGASHLLSVTAAPLQLDPHGVCARDA
HHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCCCCC
RYLVVLRPAEEEAGDAAAALATTGIISRSRAMGRVVRLVESLQYSEASVLVTGESGSGKE
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
VLARLIHAHSPRRSGPFVAVNAAALPGDLLESELFGHVRGAFTGATRDRPGRFAAAAQGT
HHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHCCCC
LFLDEVGDLPLHLQVKLLRVLQEHTYERVGENQPRRADARIIAATNRDLRRAVADGTFRE
EEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHCCCHHH
DLYYRLRVFPVEIPPLRERREDIEPMAQLLLSRVCARAGRAVRFSPDALRALMSHSWPGN
HHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCC
VRELENALEFAVTVCRGQTVQPEDLPPEVLGGGAPEHAPVRAFEPADAYERAAIEAALQA
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HRWSRAEAARALGLSRSTLWRRMRSLNIG
HHHHHHHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]