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The map label for this gene is ogt [H]

Identifier: 220916141

GI number: 220916141

Start: 1150531

End: 1151145

Strand: Reverse

Name: ogt [H]

Synonym: A2cp1_1028

Alternate gene names: 220916141

Gene position: 1151145-1150531 (Counterclockwise)

Preceding gene: 220916142

Following gene: 220916139

Centisome position: 22.89

GC content: 80.0

Gene sequence:

>615_bases
ATGACGCTCGAGCACGTGGTGGTGGAGTCGCCCATCGGCCCGGTGCACCTCGTCTCGGACGGGCGCGCGCTCTGCGCGCT
CGACCTGGGGGACACGCCGCTGCTGCGCCCGGCGCTGGCGCGGCGCTTCGGCCCCGACCTCGCGCTGCGCGCGGGCCGCG
ACCCGCTCGGCCTGGCGTCCCGCGCGCGCGCCTGGTTCGCGGGCGACCTCGGCGCGCTGGACGGGCTCCCGCTCGATCCG
GGGGGCACGCCGTTCCAGCGGCAGGTCTGGGCCGAGCTGCGCCGCATCCCGGCCGGCGAGACCCGCAGCTACGGCGCGCT
CGCGGCCGCGCTGGGGCGGCCGGGCGCGGCGCGGGCGGTGGGCCTCGCGAACGGGCGGAACCCGATCGCGATCGCCATCC
CCTGCCACCGCGTCATCGGGGCGGACGGGAGCCTGACCGGCTACGCCGGCGGCCTCGACCGGAAGCGCTGGCTGCTGGCG
CACGAGCGCGCCGCGCTGGACGGCGCCGCCCTCCGCGCGCCCACCGGCCTCGCTCAGGACCCCGGCGCGGTGGGCGGCGA
GAACAGGGCCGCGATGGCGCGGGCCACGGTCCGCCAGAGCGCCCGCACGACATAG

Upstream 100 bases:

>100_bases
GCTGGCGCCCGTGGCGGGCGTACGCGGCGCTGCACCTGTGGACGAGCCTGCGGCCCCGCGCACGCGCGGCGCCCGGCGCA
CGGAAGAAGGGGAGGCGGTC

Downstream 100 bases:

>100_bases
AGCAGCGCGGTGAGCAGCACGAGCGCCGCCGCGAGCGCGATCCAGGGGTGCGCCCAGATCGCCCAGGCGAGCGCCGCCAC
CACCCCGAGCTCGCCGGTGG

Product: methylated-DNA/protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MTLEHVVVESPIGPVHLVSDGRALCALDLGDTPLLRPALARRFGPDLALRAGRDPLGLASRARAWFAGDLGALDGLPLDP
GGTPFQRQVWAELRRIPAGETRSYGALAAALGRPGAARAVGLANGRNPIAIAIPCHRVIGADGSLTGYAGGLDRKRWLLA
HERAALDGAALRAPTGLAQDPGAVGGENRAAMARATVRQSARTT

Sequences:

>Translated_204_residues
MTLEHVVVESPIGPVHLVSDGRALCALDLGDTPLLRPALARRFGPDLALRAGRDPLGLASRARAWFAGDLGALDGLPLDP
GGTPFQRQVWAELRRIPAGETRSYGALAAALGRPGAARAVGLANGRNPIAIAIPCHRVIGADGSLTGYAGGLDRKRWLLA
HERAALDGAALRAPTGLAQDPGAVGGENRAAMARATVRQSARTT
>Mature_203_residues
TLEHVVVESPIGPVHLVSDGRALCALDLGDTPLLRPALARRFGPDLALRAGRDPLGLASRARAWFAGDLGALDGLPLDPG
GTPFQRQVWAELRRIPAGETRSYGALAAALGRPGAARAVGLANGRNPIAIAIPCHRVIGADGSLTGYAGGLDRKRWLLAH
ERAALDGAALRAPTGLAQDPGAVGGENRAAMARATVRQSARTT

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=79, Percent_Identity=54.4303797468354, Blast_Score=81, Evalue=5e-16,
Organism=Escherichia coli, GI1787596, Length=109, Percent_Identity=62.3853211009174, Blast_Score=148, Evalue=2e-37,
Organism=Escherichia coli, GI1788542, Length=87, Percent_Identity=57.4712643678161, Blast_Score=100, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI115533068, Length=89, Percent_Identity=48.314606741573, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=89, Percent_Identity=48.314606741573, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=82, Percent_Identity=45.1219512195122, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI17137554, Length=99, Percent_Identity=41.4141414141414, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 21105; Mature: 20974

Theoretical pI: Translated: 11.28; Mature: 11.28

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLEHVVVESPIGPVHLVSDGRALCALDLGDTPLLRPALARRFGPDLALRAGRDPLGLAS
CCCCCEEEECCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCHHH
RARAWFAGDLGALDGLPLDPGGTPFQRQVWAELRRIPAGETRSYGALAAALGRPGAARAV
HHHEHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEE
GLANGRNPIAIAIPCHRVIGADGSLTGYAGGLDRKRWLLAHERAALDGAALRAPTGLAQD
ECCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCC
PGAVGGENRAAMARATVRQSARTT
CCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLEHVVVESPIGPVHLVSDGRALCALDLGDTPLLRPALARRFGPDLALRAGRDPLGLAS
CCCCEEEECCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCHHH
RARAWFAGDLGALDGLPLDPGGTPFQRQVWAELRRIPAGETRSYGALAAALGRPGAARAV
HHHEHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEE
GLANGRNPIAIAIPCHRVIGADGSLTGYAGGLDRKRWLLAHERAALDGAALRAPTGLAQD
ECCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCC
PGAVGGENRAAMARATVRQSARTT
CCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2825131; 9097039; 9278503; 1520330 [H]