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The map label for this gene is camA [H]

Identifier: 220916112

GI number: 220916112

Start: 1123778

End: 1124947

Strand: Reverse

Name: camA [H]

Synonym: A2cp1_0999

Alternate gene names: 220916112

Gene position: 1124947-1123778 (Counterclockwise)

Preceding gene: 220916113

Following gene: 220916109

Centisome position: 22.37

GC content: 76.07

Gene sequence:

>1170_bases
ATGGCCGGCTTCGACTACGTGGTGGTGGGCGGCGGGATGGCGGGGCACGCCGCGGCCCAGGGGATCCGGCTGGTGGACGG
CGCCGGCTCCATCGCCATCCTGGGCGACGAGCCGGAGCGGCCCTACGCCCGGCCCCCGCTCTCCAAGGCGCTCTGGCGCG
GGCAGGAGGAGGGCTCGATCTGGCTGCCCGAGGTGGCCGGGACCACGCTCCGTACCGGCGTGCGCGTGAGCGCGATCGAC
CGCGCCGCGCACCGGGTCGAGCTCGAGGGCGGCGGGGCGATCGAGTACCGCAAGCTGCTGCTCGCGACCGGAGGGACGCC
GCGGCGGCTGCCCGGGGCCGCGGAAGGGGTGATCCACTTCCGCACCGTGGCGGACTTCCGCCGGCTGCGCGCGCTCCCGG
CCGGTCGGCGCGTGGTGGTGGTCGGCGGCGGCTTCATCGGCTCGGAGGTGTCCTCGGCGCTCTCCGACGCCGGCTACCGG
GTGACGCTCGTGTTCCCGGAGGAGACCATCGGTGCGCGGACGTTCCCGCGCGACCTGGGCCTGCACCTGAACGGCTACTA
CGGCGAGCACGGGGTGGAGGTGCTGCCGGCCACGCGGGTCTCCGGCGTGGAGCGGCGCGGGGACGGCTTCGCCGTGCGCA
CCGGCGGCGGCGAGCTCCCGGCGGACCTGGTGGTGGCGGGGCTCGGGATCGCGCCGAACGACGCGCTGGCGCGCGGCGCC
GGGCTGGACGTGGACGACGGCATCGTGGTGGACGCGTCGCTCCGCACCCGCGATCCGGACGTGTTCGCCGCGGGCGACGT
GGCGCGCTTCTGGAACCCCGCGCTGGGCCGGCTCCTCCGCGTGGAGCACGAGGACAACGCGAACAAGATGGGCGAGACGG
CGGGGCGCGCCATGGCCGGCGCGGACGTCGTCTACGGCCACCTCCCGTTCTTCTACTCCGACCTGTTCGACCTCGGGTAC
GAGGCGGTCGGCGTGGTGGACGCGCGGCTCGAGACCGTCGCCGACTGGCAGGAGCCGTTCCGCCGGGGCGTGATCTACTA
CCTCGCCGACGGGCGCGTCCGGGGCGTGGTGACCTGGGGCGCGTTCGGCAAGCTGGACGCCGCCCGCGCGCTGGTGGCCG
AGCAGGGGCCGCACCGCGCGGCCGACCTGCGCGGCCGGATCCCGGTGTAG

Upstream 100 bases:

>100_bases
CCAGGGCGCGCACCCGGCCCTCCCGTAACGCGATCCCCGCGGCCGCATCCACTATGGGGTTCGCAGCGGCGCCCGGCGGC
GCCGCGCCGCGGAGGACGCG

Downstream 100 bases:

>100_bases
CGGCGCCGCCTCGCCGCGTCAGCGCGGACGATCGCGGTCGAGGATGGCGAAGGGCCGCTCGCCCTTGAAGACGCGGAACA
GCAGGTAGAGCGCCGGGAAC

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MAGFDYVVVGGGMAGHAAAQGIRLVDGAGSIAILGDEPERPYARPPLSKALWRGQEEGSIWLPEVAGTTLRTGVRVSAID
RAAHRVELEGGGAIEYRKLLLATGGTPRRLPGAAEGVIHFRTVADFRRLRALPAGRRVVVVGGGFIGSEVSSALSDAGYR
VTLVFPEETIGARTFPRDLGLHLNGYYGEHGVEVLPATRVSGVERRGDGFAVRTGGGELPADLVVAGLGIAPNDALARGA
GLDVDDGIVVDASLRTRDPDVFAAGDVARFWNPALGRLLRVEHEDNANKMGETAGRAMAGADVVYGHLPFFYSDLFDLGY
EAVGVVDARLETVADWQEPFRRGVIYYLADGRVRGVVTWGAFGKLDAARALVAEQGPHRAADLRGRIPV

Sequences:

>Translated_389_residues
MAGFDYVVVGGGMAGHAAAQGIRLVDGAGSIAILGDEPERPYARPPLSKALWRGQEEGSIWLPEVAGTTLRTGVRVSAID
RAAHRVELEGGGAIEYRKLLLATGGTPRRLPGAAEGVIHFRTVADFRRLRALPAGRRVVVVGGGFIGSEVSSALSDAGYR
VTLVFPEETIGARTFPRDLGLHLNGYYGEHGVEVLPATRVSGVERRGDGFAVRTGGGELPADLVVAGLGIAPNDALARGA
GLDVDDGIVVDASLRTRDPDVFAAGDVARFWNPALGRLLRVEHEDNANKMGETAGRAMAGADVVYGHLPFFYSDLFDLGY
EAVGVVDARLETVADWQEPFRRGVIYYLADGRVRGVVTWGAFGKLDAARALVAEQGPHRAADLRGRIPV
>Mature_388_residues
AGFDYVVVGGGMAGHAAAQGIRLVDGAGSIAILGDEPERPYARPPLSKALWRGQEEGSIWLPEVAGTTLRTGVRVSAIDR
AAHRVELEGGGAIEYRKLLLATGGTPRRLPGAAEGVIHFRTVADFRRLRALPAGRRVVVVGGGFIGSEVSSALSDAGYRV
TLVFPEETIGARTFPRDLGLHLNGYYGEHGVEVLPATRVSGVERRGDGFAVRTGGGELPADLVVAGLGIAPNDALARGAG
LDVDDGIVVDASLRTRDPDVFAAGDVARFWNPALGRLLRVEHEDNANKMGETAGRAMAGADVVYGHLPFFYSDLFDLGYE
AVGVVDARLETVADWQEPFRRGVIYYLADGRVRGVVTWGAFGKLDAARALVAEQGPHRAADLRGRIPV

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI22202629, Length=467, Percent_Identity=31.0492505353319, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI4757732, Length=467, Percent_Identity=31.0492505353319, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI226437568, Length=327, Percent_Identity=30.8868501529052, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI65787454, Length=327, Percent_Identity=30.8868501529052, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI21389617, Length=327, Percent_Identity=30.8868501529052, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI22202631, Length=274, Percent_Identity=30.2919708029197, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI195927004, Length=242, Percent_Identity=30.9917355371901, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI195927006, Length=186, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1788892, Length=406, Percent_Identity=31.0344827586207, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789065, Length=274, Percent_Identity=30.2919708029197, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789765, Length=294, Percent_Identity=29.2517006802721, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI32564386, Length=430, Percent_Identity=29.0697674418605, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17559934, Length=330, Percent_Identity=28.4848484848485, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28573993, Length=471, Percent_Identity=28.6624203821656, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24581020, Length=471, Percent_Identity=28.6624203821656, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24639257, Length=332, Percent_Identity=26.2048192771084, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281359715, Length=332, Percent_Identity=26.2048192771084, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI281359713, Length=332, Percent_Identity=26.2048192771084, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24639250, Length=332, Percent_Identity=26.2048192771084, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI18543267, Length=332, Percent_Identity=26.2048192771084, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24639252, Length=332, Percent_Identity=26.2048192771084, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24585130, Length=350, Percent_Identity=24, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 41208; Mature: 41077

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGFDYVVVGGGMAGHAAAQGIRLVDGAGSIAILGDEPERPYARPPLSKALWRGQEEGSI
CCCCCEEEECCCCCCHHHHCCEEEEECCCCEEEECCCCCCCCCCCCHHHHHHCCCCCCCE
WLPEVAGTTLRTGVRVSAIDRAAHRVELEGGGAIEYRKLLLATGGTPRRLPGAAEGVIHF
ECCCCCCCHHHCCEEEEEEHHCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCEEEE
RTVADFRRLRALPAGRRVVVVGGGFIGSEVSSALSDAGYRVTLVFPEETIGARTFPRDLG
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEECCHHCCCCCCCHHCC
LHLNGYYGEHGVEVLPATRVSGVERRGDGFAVRTGGGELPADLVVAGLGIAPNDALARGA
CEEECEECCCCCEEECCHHHCCCCCCCCCEEEECCCCCCCHHHHEEECCCCCCHHHHCCC
GLDVDDGIVVDASLRTRDPDVFAAGDVARFWNPALGRLLRVEHEDNANKMGETAGRAMAG
CCCCCCCEEEEEECCCCCCCEEECCCHHHHHCHHHHHEEEEECCCCCHHHHHHHHHHHCC
ADVVYGHLPFFYSDLFDLGYEAVGVVDARLETVADWQEPFRRGVIYYLADGRVRGVVTWG
CCEEEECCHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEEEC
AFGKLDAARALVAEQGPHRAADLRGRIPV
CCCCHHHHHHHHHCCCCCCHHCCCCCCCC
>Mature Secondary Structure 
AGFDYVVVGGGMAGHAAAQGIRLVDGAGSIAILGDEPERPYARPPLSKALWRGQEEGSI
CCCCEEEECCCCCCHHHHCCEEEEECCCCEEEECCCCCCCCCCCCHHHHHHCCCCCCCE
WLPEVAGTTLRTGVRVSAIDRAAHRVELEGGGAIEYRKLLLATGGTPRRLPGAAEGVIHF
ECCCCCCCHHHCCEEEEEEHHCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCEEEE
RTVADFRRLRALPAGRRVVVVGGGFIGSEVSSALSDAGYRVTLVFPEETIGARTFPRDLG
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEECCHHCCCCCCCHHCC
LHLNGYYGEHGVEVLPATRVSGVERRGDGFAVRTGGGELPADLVVAGLGIAPNDALARGA
CEEECEECCCCCEEECCHHHCCCCCCCCCEEEECCCCCCCHHHHEEECCCCCCHHHHCCC
GLDVDDGIVVDASLRTRDPDVFAAGDVARFWNPALGRLLRVEHEDNANKMGETAGRAMAG
CCCCCCCEEEEEECCCCCCCEEECCCHHHHHCHHHHHEEEEECCCCCHHHHHHHHHHHCC
ADVVYGHLPFFYSDLFDLGYEAVGVVDARLETVADWQEPFRRGVIYYLADGRVRGVVTWG
CCEEEECCHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEEEC
AFGKLDAARALVAEQGPHRAADLRGRIPV
CCCCHHHHHHHHHCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]