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The map label for this gene is mdeA [H]

Identifier: 220916093

GI number: 220916093

Start: 1105908

End: 1107227

Strand: Reverse

Name: mdeA [H]

Synonym: A2cp1_0980

Alternate gene names: 220916093

Gene position: 1107227-1105908 (Counterclockwise)

Preceding gene: 220916095

Following gene: 220916089

Centisome position: 22.02

GC content: 69.02

Gene sequence:

>1320_bases
ATGGCCATCCCCCCGAACAGGAAGCTCCGCTTCGAGACGCTGCAGGTCCACGCCGGCCAGGAGCCCGCGCCGGGGACGAA
CGCCCGCGCGGTCCCCATCTACCAGACGTCGTCCTACACCTTCGACTCGGCGGAGCACGGCGCGAACCTGTTCGCGCTGA
AGGAGTTCGGGAACATCTACACGCGGATCATGAACCCGACCACGGACGTGTTCGAGAAGCGCATCGCCGCGCTCGAGGGC
GGCGTCGCCGCGCTCGCCACGTCCTCGGGCCAGGCCGCGCAGTTCCTCGCCATCGCGAACCTGGCGCAGGCCGGCGACAA
CGTGGTCTCGACCAGCAACCTGTACGGCGGCACGTACAACCAGTTCAAGGTCACGCTCCCCCGGCTCGGCATCGACGTGA
AGCTGGTGGAGGGCGCCGAGGTGGACACGATCCGCAAGGCGATCGACGGGAAGACGAAGGCGGTCTACGTCGAGTCCATC
GGCAACCCCGCCGGCAACGTCCCCGACTTCGAGGCGCTGGCCGCGCTCGCGCACGACAACGGCATCCCGCTGCTGGTGGA
CAACACCTTCGGCGCCGGCGGCTACTTCTGCCGGCCCATCGAGTGGGGCGCCGACGTGGTGGTGGAGTCCGCCACGAAGT
GGATCGGCGGCCACGGCACCTCGATCGGCGGCGTGATCGTGGACTCGGGCAAGTTCGACTGGGCGAAGAGCGGCAAGTTC
CCGTTCTTCACCGAGCCGTCCCCGGGCTACCACGGCCTGGTGTTCAACGACGTGTTCGGCCCGAAGGGCCCGTTCGGCAA
CATCCAGTTCGTCCTGCGCGCCCGCGTCGAGGGGCTGCGCGACCTCGGCCCGACGCTCTCGCCGTTCAACGCGTTCCTGC
TGCTGCAAGGCGTCGAGACGCTCTCCCTGCGCGTGCAGCGCATCGGCGAGAACACGCTCGCGCTGGCACGCTGGCTGAAG
TCGCACCCGGACGTCGCCTGGGTGAACTACACCGGCCTCGAGGAGCACCCGTTCCACCAGCGGGCCCGCAAGTACCTGCG
GAACGGGTTCGGCGGCGTCTTCACCTTCGGCGTGAAGGGCGGCTACGAGGCGGGCAAGGCGTTCATCGACTCGGTGAAGC
TCGCCTCGCACCTCGCGAACGTCGGCGACGCGAAGACGCTCGTGATCCACCCGGCCTCGACCACCCACCAGCAGCTCTCC
GACGCCGAGCAGCAGGGCTCCGGCGTCACGCCGGACCAGATCCGCGTCTCGCTGGGCATCGAGCACATCGAGGACATCAA
GGAGGACTTCGACGAGGCCCTCCGCGCCACGCGGCGGTAG

Upstream 100 bases:

>100_bases
CCTCGATGGCCGCATTACGCCCGACCGGCTTGACATCCCGCGCGTCCGCTTCTTAGGATGACCATCCAACTCAACGGACC
GCCCCCGCCCAGAGGAACGC

Downstream 100 bases:

>100_bases
CCCCTCCCTCCCGCCGCGCCGCGCCCCGGCCCCTCCTGCCCTGCGGCGGGAGGGGCCGTTCCGTCCGCGGCGGCGCTCAG
AGCGCGTGGAATCCATCCCC

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MAIPPNRKLRFETLQVHAGQEPAPGTNARAVPIYQTSSYTFDSAEHGANLFALKEFGNIYTRIMNPTTDVFEKRIAALEG
GVAALATSSGQAAQFLAIANLAQAGDNVVSTSNLYGGTYNQFKVTLPRLGIDVKLVEGAEVDTIRKAIDGKTKAVYVESI
GNPAGNVPDFEALAALAHDNGIPLLVDNTFGAGGYFCRPIEWGADVVVESATKWIGGHGTSIGGVIVDSGKFDWAKSGKF
PFFTEPSPGYHGLVFNDVFGPKGPFGNIQFVLRARVEGLRDLGPTLSPFNAFLLLQGVETLSLRVQRIGENTLALARWLK
SHPDVAWVNYTGLEEHPFHQRARKYLRNGFGGVFTFGVKGGYEAGKAFIDSVKLASHLANVGDAKTLVIHPASTTHQQLS
DAEQQGSGVTPDQIRVSLGIEHIEDIKEDFDEALRATRR

Sequences:

>Translated_439_residues
MAIPPNRKLRFETLQVHAGQEPAPGTNARAVPIYQTSSYTFDSAEHGANLFALKEFGNIYTRIMNPTTDVFEKRIAALEG
GVAALATSSGQAAQFLAIANLAQAGDNVVSTSNLYGGTYNQFKVTLPRLGIDVKLVEGAEVDTIRKAIDGKTKAVYVESI
GNPAGNVPDFEALAALAHDNGIPLLVDNTFGAGGYFCRPIEWGADVVVESATKWIGGHGTSIGGVIVDSGKFDWAKSGKF
PFFTEPSPGYHGLVFNDVFGPKGPFGNIQFVLRARVEGLRDLGPTLSPFNAFLLLQGVETLSLRVQRIGENTLALARWLK
SHPDVAWVNYTGLEEHPFHQRARKYLRNGFGGVFTFGVKGGYEAGKAFIDSVKLASHLANVGDAKTLVIHPASTTHQQLS
DAEQQGSGVTPDQIRVSLGIEHIEDIKEDFDEALRATRR
>Mature_438_residues
AIPPNRKLRFETLQVHAGQEPAPGTNARAVPIYQTSSYTFDSAEHGANLFALKEFGNIYTRIMNPTTDVFEKRIAALEGG
VAALATSSGQAAQFLAIANLAQAGDNVVSTSNLYGGTYNQFKVTLPRLGIDVKLVEGAEVDTIRKAIDGKTKAVYVESIG
NPAGNVPDFEALAALAHDNGIPLLVDNTFGAGGYFCRPIEWGADVVVESATKWIGGHGTSIGGVIVDSGKFDWAKSGKFP
FFTEPSPGYHGLVFNDVFGPKGPFGNIQFVLRARVEGLRDLGPTLSPFNAFLLLQGVETLSLRVQRIGENTLALARWLKS
HPDVAWVNYTGLEEHPFHQRARKYLRNGFGGVFTFGVKGGYEAGKAFIDSVKLASHLANVGDAKTLVIHPASTTHQQLSD
AEQQGSGVTPDQIRVSLGIEHIEDIKEDFDEALRATRR

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=429, Percent_Identity=31.002331002331, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI299473759, Length=429, Percent_Identity=28.2051282051282, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI25453487, Length=428, Percent_Identity=26.6355140186916, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1790375, Length=408, Percent_Identity=28.1862745098039, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1789383, Length=431, Percent_Identity=24.8259860788863, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71980708, Length=441, Percent_Identity=31.9727891156463, Blast_Score=201, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17538055, Length=431, Percent_Identity=32.7146171693735, Blast_Score=184, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17560056, Length=430, Percent_Identity=32.7906976744186, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323333, Length=440, Percent_Identity=58.4090909090909, Blast_Score=468, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6319307, Length=382, Percent_Identity=31.9371727748691, Blast_Score=166, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6321254, Length=452, Percent_Identity=25.8849557522124, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321170, Length=307, Percent_Identity=25.4071661237785, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321904, Length=397, Percent_Identity=24.6851385390428, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19922554, Length=432, Percent_Identity=30.3240740740741, Blast_Score=189, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47339; Mature: 47208

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPPNRKLRFETLQVHAGQEPAPGTNARAVPIYQTSSYTFDSAEHGANLFALKEFGNIY
CCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHCCCCEEEEHHHHHHH
TRIMNPTTDVFEKRIAALEGGVAALATSSGQAAQFLAIANLAQAGDNVVSTSNLYGGTYN
HHHCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCC
QFKVTLPRLGIDVKLVEGAEVDTIRKAIDGKTKAVYVESIGNPAGNVPDFEALAALAHDN
EEEEEECCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCC
GIPLLVDNTFGAGGYFCRPIEWGADVVVESATKWIGGHGTSIGGVIVDSGKFDWAKSGKF
CCEEEEECCCCCCCEEECEECCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCC
PFFTEPSPGYHGLVFNDVFGPKGPFGNIQFVLRARVEGLRDLGPTLSPFNAFLLLQGVET
CEECCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHH
LSLRVQRIGENTLALARWLKSHPDVAWVNYTGLEEHPFHQRARKYLRNGFGGVFTFGVKG
HHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCC
GYEAGKAFIDSVKLASHLANVGDAKTLVIHPASTTHQQLSDAEQQGSGVTPDQIRVSLGI
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHEEEEECH
EHIEDIKEDFDEALRATRR
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AIPPNRKLRFETLQVHAGQEPAPGTNARAVPIYQTSSYTFDSAEHGANLFALKEFGNIY
CCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHCCCCEEEEHHHHHHH
TRIMNPTTDVFEKRIAALEGGVAALATSSGQAAQFLAIANLAQAGDNVVSTSNLYGGTYN
HHHCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCC
QFKVTLPRLGIDVKLVEGAEVDTIRKAIDGKTKAVYVESIGNPAGNVPDFEALAALAHDN
EEEEEECCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCC
GIPLLVDNTFGAGGYFCRPIEWGADVVVESATKWIGGHGTSIGGVIVDSGKFDWAKSGKF
CCEEEEECCCCCCCEEECEECCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCC
PFFTEPSPGYHGLVFNDVFGPKGPFGNIQFVLRARVEGLRDLGPTLSPFNAFLLLQGVET
CEECCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHH
LSLRVQRIGENTLALARWLKSHPDVAWVNYTGLEEHPFHQRARKYLRNGFGGVFTFGVKG
HHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCC
GYEAGKAFIDSVKLASHLANVGDAKTLVIHPASTTHQQLSDAEQQGSGVTPDQIRVSLGI
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHEEEEECH
EHIEDIKEDFDEALRATRR
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]