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The map label for this gene is nrfA [H]

Identifier: 220916076

GI number: 220916076

Start: 1082950

End: 1084422

Strand: Reverse

Name: nrfA [H]

Synonym: A2cp1_0963

Alternate gene names: 220916076

Gene position: 1084422-1082950 (Counterclockwise)

Preceding gene: 220916077

Following gene: 220916075

Centisome position: 21.56

GC content: 70.06

Gene sequence:

>1473_bases
ATGACCGTCGAGACCCAGTCCCCGAGGAAGCGCGCGCTCGTCGTCGGCGCGATCGCCGCCGTCTTCGCGCTCGCCGCCGT
CCTCGCCGCCGTGGTCCTGGTGGACGTCGCCCAGAAGAAGGCCGAGGCGCGCAACCCCGTGGTGCGCGTCGTCGAGCTCA
CCGACGAGACCGAGGACCCCGCCGTGTGGGGGAGGAACTTCCCCATCGAGTACGACCTCTACCGGAAGACCACCGACATG
GTCCGGACCAAGCACGGCGGCTCGGAGGCGATGCCGCACGCGCCCGGCGACGGCGACCCGCGCACCGTCACCGCCCAGTC
CAAGACCGACGCGGACCCGCGCCTGAAGACCATGTGGGCCGGCTACGCGTTCGCCGCCGACTTCCGCGAGGAGCGCGGCC
ACGCGTACATGCTCGACGACCAGTTCTTCACCCAGCGCCAGGTGGTCGCGAAGCAGCCCGGCACCTGCGGCCAGTGCCAC
GCGTCGGTGTACGTCCCGATGAAGAAGCTCGGGAACGGCGACCTGTTCAAGGGCTTCGAGGCCCTGAACAAGATGCCGTA
CTCCGAGGCGAAGCAGCACTTCAAGCACCCGGTGTCCTGCATCGACTGCCACGACCCGACCACCATGGACCTGCGCGTCA
CGCGCCCCGGGTTCCTGGAGGGCATCCGCGCGTACAAGGCGTCGCTCGGCGTGAAGGACTACGACCCGAACACCATGGCC
ACGCGGCAAGAGATGCGCGCGTACGTCTGCGGCCAGTGCCACGTCGAGTACTACTTCAAGGGGCCCGAGAAGCGGCTCAC
CTTCCCCTGGTCGAAGGGGCTGAAGGCCGACCAGATCGTCTCCTACTACGACGAGGTCGGGTTCAAGGACTGGGTGCACA
AGGAGAGCGGCGCGCCGGCGCTCAAGGCGCAGCACCCCGAGTTCGAGATGTGGAACCAGGGCATCCACGCCCGCTCCGGC
GTGGCCTGCGCCGACTGCCACATGCCGTACATGCGGGTGGGCGGCACCAAGGTGTCGGACCACCAGGTGCGCAGCCCGGT
GCTGAACCTGAACCGCGCCTGCCAGGGCTGCCACAAGTGGCCGGAGGCGGAGCTGAAGGGCCGCGTCGAGGCGATCCAGG
ACAAGCACATGGAGCTCCGCGAGCAGGCCTTCGACGCGCTGGTCGCGCTCATCGGCGAGATCAAGGCCGCGCGCGAGGCG
GGCAAGGCCGACGAGCAGCTGGTCACCGCCCGGTACCTGCAGCGCCGCGCGCAGTTCCTGTTCGACTTCGTCGAGGCCGA
GAACTCCACCGGCTTCCACGCGCCGCAGGAGGCCGCCCGCGTGCTGTTCGAGTCCATCGACGCCTCGCGCCAGGGCCAGC
TCGCGCTGCGCGACCCGTCCTTCAAGCCGACCGTGGCGGTGGTGAACGTCTCCACCGCGCCTGCGCCCGCCGCGCCCGCC
GGCCCGGCGGTGGTCCCCGCGGTGCTGAAGTAG

Upstream 100 bases:

>100_bases
ACCGGCGGCCGGGACGGCGTCTCGTGCATCCGCTGCCACGCCTCCGTCGGACACCTCCGCTGACCCACCGCCTCACGCCG
CACTCCAAGGAGAACCCGCA

Downstream 100 bases:

>100_bases
CAGCCGGGCCCCGCCGCCGGCCGCCCCGTCCCCCACTCCCCCGGGGCGGCCGGCGCGCGGGGACCTGGACGCCGTTCCAC
CCGTCGTCCCGAAGGAGTCG

Product: Nitrite reductase

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTVETQSPRKRALVVGAIAAVFALAAVLAAVVLVDVAQKKAEARNPVVRVVELTDETEDPAVWGRNFPIEYDLYRKTTDM
VRTKHGGSEAMPHAPGDGDPRTVTAQSKTDADPRLKTMWAGYAFAADFREERGHAYMLDDQFFTQRQVVAKQPGTCGQCH
ASVYVPMKKLGNGDLFKGFEALNKMPYSEAKQHFKHPVSCIDCHDPTTMDLRVTRPGFLEGIRAYKASLGVKDYDPNTMA
TRQEMRAYVCGQCHVEYYFKGPEKRLTFPWSKGLKADQIVSYYDEVGFKDWVHKESGAPALKAQHPEFEMWNQGIHARSG
VACADCHMPYMRVGGTKVSDHQVRSPVLNLNRACQGCHKWPEAELKGRVEAIQDKHMELREQAFDALVALIGEIKAAREA
GKADEQLVTARYLQRRAQFLFDFVEAENSTGFHAPQEAARVLFESIDASRQGQLALRDPSFKPTVAVVNVSTAPAPAAPA
GPAVVPAVLK

Sequences:

>Translated_490_residues
MTVETQSPRKRALVVGAIAAVFALAAVLAAVVLVDVAQKKAEARNPVVRVVELTDETEDPAVWGRNFPIEYDLYRKTTDM
VRTKHGGSEAMPHAPGDGDPRTVTAQSKTDADPRLKTMWAGYAFAADFREERGHAYMLDDQFFTQRQVVAKQPGTCGQCH
ASVYVPMKKLGNGDLFKGFEALNKMPYSEAKQHFKHPVSCIDCHDPTTMDLRVTRPGFLEGIRAYKASLGVKDYDPNTMA
TRQEMRAYVCGQCHVEYYFKGPEKRLTFPWSKGLKADQIVSYYDEVGFKDWVHKESGAPALKAQHPEFEMWNQGIHARSG
VACADCHMPYMRVGGTKVSDHQVRSPVLNLNRACQGCHKWPEAELKGRVEAIQDKHMELREQAFDALVALIGEIKAAREA
GKADEQLVTARYLQRRAQFLFDFVEAENSTGFHAPQEAARVLFESIDASRQGQLALRDPSFKPTVAVVNVSTAPAPAAPA
GPAVVPAVLK
>Mature_489_residues
TVETQSPRKRALVVGAIAAVFALAAVLAAVVLVDVAQKKAEARNPVVRVVELTDETEDPAVWGRNFPIEYDLYRKTTDMV
RTKHGGSEAMPHAPGDGDPRTVTAQSKTDADPRLKTMWAGYAFAADFREERGHAYMLDDQFFTQRQVVAKQPGTCGQCHA
SVYVPMKKLGNGDLFKGFEALNKMPYSEAKQHFKHPVSCIDCHDPTTMDLRVTRPGFLEGIRAYKASLGVKDYDPNTMAT
RQEMRAYVCGQCHVEYYFKGPEKRLTFPWSKGLKADQIVSYYDEVGFKDWVHKESGAPALKAQHPEFEMWNQGIHARSGV
ACADCHMPYMRVGGTKVSDHQVRSPVLNLNRACQGCHKWPEAELKGRVEAIQDKHMELREQAFDALVALIGEIKAAREAG
KADEQLVTARYLQRRAQFLFDFVEAENSTGFHAPQEAARVLFESIDASRQGQLALRDPSFKPTVAVVNVSTAPAPAAPAG
PAVVPAVLK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=353, Percent_Identity=33.1444759206799, Blast_Score=202, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 54306; Mature: 54175

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00369 PTS_HPR_HIS ; PS00189 LIPOYL ; PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVETQSPRKRALVVGAIAAVFALAAVLAAVVLVDVAQKKAEARNPVVRVVELTDETEDP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
AVWGRNFPIEYDLYRKTTDMVRTKHGGSEAMPHAPGDGDPRTVTAQSKTDADPRLKTMWA
CCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHH
GYAFAADFREERGHAYMLDDQFFTQRQVVAKQPGTCGQCHASVYVPMKKLGNGDLFKGFE
HHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCEEEEEHHHCCCCHHHHHHH
ALNKMPYSEAKQHFKHPVSCIDCHDPTTMDLRVTRPGFLEGIRAYKASLGVKDYDPNTMA
HHHCCCHHHHHHHHCCCCCEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHH
TRQEMRAYVCGQCHVEYYFKGPEKRLTFPWSKGLKADQIVSYYDEVGFKDWVHKESGAPA
HHHHHHHHHCCEEEEEEEEECCCCEEECCHHCCCCHHHHHHHHHHCCHHHHHHCCCCCCC
LKAQHPEFEMWNQGIHARSGVACADCHMPYMRVGGTKVSDHQVRSPVLNLNRACQGCHKW
CCCCCCCHHHHHCCCCCCCCCEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
PEAELKGRVEAIQDKHMELREQAFDALVALIGEIKAAREAGKADEQLVTARYLQRRAQFL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
FDFVEAENSTGFHAPQEAARVLFESIDASRQGQLALRDPSFKPTVAVVNVSTAPAPAAPA
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCC
GPAVVPAVLK
CCCCHHHHCC
>Mature Secondary Structure 
TVETQSPRKRALVVGAIAAVFALAAVLAAVVLVDVAQKKAEARNPVVRVVELTDETEDP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
AVWGRNFPIEYDLYRKTTDMVRTKHGGSEAMPHAPGDGDPRTVTAQSKTDADPRLKTMWA
CCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHH
GYAFAADFREERGHAYMLDDQFFTQRQVVAKQPGTCGQCHASVYVPMKKLGNGDLFKGFE
HHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCEEEEEHHHCCCCHHHHHHH
ALNKMPYSEAKQHFKHPVSCIDCHDPTTMDLRVTRPGFLEGIRAYKASLGVKDYDPNTMA
HHHCCCHHHHHHHHCCCCCEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHH
TRQEMRAYVCGQCHVEYYFKGPEKRLTFPWSKGLKADQIVSYYDEVGFKDWVHKESGAPA
HHHHHHHHHCCEEEEEEEEECCCCEEECCHHCCCCHHHHHHHHHHCCHHHHHHCCCCCCC
LKAQHPEFEMWNQGIHARSGVACADCHMPYMRVGGTKVSDHQVRSPVLNLNRACQGCHKW
CCCCCCCHHHHHCCCCCCCCCEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
PEAELKGRVEAIQDKHMELREQAFDALVALIGEIKAAREAGKADEQLVTARYLQRRAQFL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
FDFVEAENSTGFHAPQEAARVLFESIDASRQGQLALRDPSFKPTVAVVNVSTAPAPAAPA
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCC
GPAVVPAVLK
CCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA