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The map label for this gene is dapE [H]

Identifier: 220916026

GI number: 220916026

Start: 1031647

End: 1033044

Strand: Direct

Name: dapE [H]

Synonym: A2cp1_0912

Alternate gene names: 220916026

Gene position: 1031647-1033044 (Clockwise)

Preceding gene: 220916022

Following gene: 220916027

Centisome position: 20.51

GC content: 73.46

Gene sequence:

>1398_bases
ATGCCCGAGACGCCGGTCGACCTGGCCCTGGCCCACTACGAGAAGAACGCCGCGAACTACCTGGACGAGCTGAAGCGGCT
GGTCCGCATCCCCAGCGTCTCGTTCCCGGGCTTCCCGGAGAACGAGGTGCGGCGCAGCGCCGAGGCGACCGCCGAGCTCC
TGCGGCGGCGCGGCTTCGAGAAGGTCGAGGTCCTCGAGGTCGAGGGCGCGCACCCGTACGTCTTCGGGGAGCGCGTCGAG
GATCCCGCCGCGCCCACCCTGCTGCTGTACGCGCACCACGACGTGCAGCCCGCCGGCGAGGCGGAGGCCTGGAAGACCCC
GCCGTTCGAGCCCACCGAGCGCGACGGCCGCCTGTGGGGCCGCGGCGCCGCCGACGACAAGGCCGGCATCATCGTGCACG
CGGCGGCGGTGGACTCCTGGGTGCGCGGCGCCCGGCGCATGCCGCTCAACGTGAAGATCGTGGTCGAGGGCGAGGAGGAG
ATCGGCTCCGATCACCTGACGGCGTTCCTCACCCGCTACCGCTCGCGCCTCGACGCCGACGCGATGGTGCTCACCGACAC
CGGCAACGTGGATTCGGGCGTGCCGTCGATCACGGTGGCGCTCCGCGGCCTGGTGGTCCTCGAGGTGGAGGTGCGCGCGC
TCGAGCAGAGCGTCCACTCCGGCATGTGGGGCGGCCCGGTGCCGGATCCCACCATGGCGCTCGCCAAGATGCTCGCCGCG
CTGGTGGACGACGACGGGGCCATCGCCATCCCCGGGATCCACGACCGCGTCCGCCCGCTCACCCGCGAGCAGCGCGAGGC
CATCGCCGCGCTGCCGGTCACCGAGGAGGACATCCGGCGGCAGGCCCGGCTGCGGCCCGGCGTGCGGCTGCTGGGCGGGC
GCCATCCGCTGGAGACGAACTGGTGGCAGCCGTCGCTCGCGGTGAACGCCATCCAGGCGTCCTCGCGCAAGGACGCGCGC
AACATCATCAACGACGTGGCCTGGGCGCGCCTCGGCGTGCGGCTGGTCCCGGACATGGACCCGGTGGACGTGCGCGAGAA
GCTCGTCGCGGCGCTGCACGCCGCAGCGCCCTGGGGCGTGGAGGTGACCGTGAAGCCGGACACCGCCGGCGCGCCCTGGA
TCACCGACCTCGGGCACCCGGCGTTCGACGCCGCGTTCCGGGCCCTGGAGAAGGGGTTCGGGCGCCCCGCCCTCGCGATC
GGGTGCGGCGGGTCGATCGGGTTCGTGGAGCCGTTCGCCAAGGCGCTCGGCGGCGTCCCGGCCCTGCTCATCGGCGTCGA
GGATCCGTCGTCGAACGCGCACTCGGAGAACGAGAGCCTGCTGCTCTCCGACTTCCAGAAGGCCATCCGGAGCGCGATCC
ACCTCTACGAGGAGCTGGCCGGCGCGCTGCGCCGGTAG

Upstream 100 bases:

>100_bases
GTCCCGAAGGGGTCGAGGGATCCCGGCCATGCGCCCTTATATCCCGGGCCGCCCCTCGCCCGCCCGCGGCGCGGGAACCC
TTCTGCCCGAGGTGACATCC

Downstream 100 bases:

>100_bases
CGCCCGGCCGCGCGCTCCGCCGGTGCGACGCGGGGTCCGCCCCGCCGCGCCGCGGCGGGGATGCGGCTATGATGTCCCGC
CGCCCGCCGTGGCCGCCGCC

Product: peptidase M20

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MPETPVDLALAHYEKNAANYLDELKRLVRIPSVSFPGFPENEVRRSAEATAELLRRRGFEKVEVLEVEGAHPYVFGERVE
DPAAPTLLLYAHHDVQPAGEAEAWKTPPFEPTERDGRLWGRGAADDKAGIIVHAAAVDSWVRGARRMPLNVKIVVEGEEE
IGSDHLTAFLTRYRSRLDADAMVLTDTGNVDSGVPSITVALRGLVVLEVEVRALEQSVHSGMWGGPVPDPTMALAKMLAA
LVDDDGAIAIPGIHDRVRPLTREQREAIAALPVTEEDIRRQARLRPGVRLLGGRHPLETNWWQPSLAVNAIQASSRKDAR
NIINDVAWARLGVRLVPDMDPVDVREKLVAALHAAAPWGVEVTVKPDTAGAPWITDLGHPAFDAAFRALEKGFGRPALAI
GCGGSIGFVEPFAKALGGVPALLIGVEDPSSNAHSENESLLLSDFQKAIRSAIHLYEELAGALRR

Sequences:

>Translated_465_residues
MPETPVDLALAHYEKNAANYLDELKRLVRIPSVSFPGFPENEVRRSAEATAELLRRRGFEKVEVLEVEGAHPYVFGERVE
DPAAPTLLLYAHHDVQPAGEAEAWKTPPFEPTERDGRLWGRGAADDKAGIIVHAAAVDSWVRGARRMPLNVKIVVEGEEE
IGSDHLTAFLTRYRSRLDADAMVLTDTGNVDSGVPSITVALRGLVVLEVEVRALEQSVHSGMWGGPVPDPTMALAKMLAA
LVDDDGAIAIPGIHDRVRPLTREQREAIAALPVTEEDIRRQARLRPGVRLLGGRHPLETNWWQPSLAVNAIQASSRKDAR
NIINDVAWARLGVRLVPDMDPVDVREKLVAALHAAAPWGVEVTVKPDTAGAPWITDLGHPAFDAAFRALEKGFGRPALAI
GCGGSIGFVEPFAKALGGVPALLIGVEDPSSNAHSENESLLLSDFQKAIRSAIHLYEELAGALRR
>Mature_464_residues
PETPVDLALAHYEKNAANYLDELKRLVRIPSVSFPGFPENEVRRSAEATAELLRRRGFEKVEVLEVEGAHPYVFGERVED
PAAPTLLLYAHHDVQPAGEAEAWKTPPFEPTERDGRLWGRGAADDKAGIIVHAAAVDSWVRGARRMPLNVKIVVEGEEEI
GSDHLTAFLTRYRSRLDADAMVLTDTGNVDSGVPSITVALRGLVVLEVEVRALEQSVHSGMWGGPVPDPTMALAKMLAAL
VDDDGAIAIPGIHDRVRPLTREQREAIAALPVTEEDIRRQARLRPGVRLLGGRHPLETNWWQPSLAVNAIQASSRKDARN
IINDVAWARLGVRLVPDMDPVDVREKLVAALHAAAPWGVEVTVKPDTAGAPWITDLGHPAFDAAFRALEKGFGRPALAIG
CGGSIGFVEPFAKALGGVPALLIGVEDPSSNAHSENESLLLSDFQKAIRSAIHLYEELAGALRR

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=470, Percent_Identity=30.2127659574468, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI21071039, Length=397, Percent_Identity=28.9672544080605, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI271398277, Length=323, Percent_Identity=29.7213622291022, Blast_Score=131, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17563146, Length=476, Percent_Identity=30.2521008403361, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI193211059, Length=474, Percent_Identity=29.3248945147679, Blast_Score=178, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6321159, Length=467, Percent_Identity=26.5524625267666, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319758, Length=453, Percent_Identity=25.1655629139073, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24585880, Length=472, Percent_Identity=30.7203389830508, Blast_Score=199, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 50496; Mature: 50365

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00141 ASP_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETPVDLALAHYEKNAANYLDELKRLVRIPSVSFPGFPENEVRRSAEATAELLRRRGFE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
KVEVLEVEGAHPYVFGERVEDPAAPTLLLYAHHDVQPAGEAEAWKTPPFEPTERDGRLWG
EEEEEEECCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEC
RGAADDKAGIIVHAAAVDSWVRGARRMPLNVKIVVEGEEEIGSDHLTAFLTRYRSRLDAD
CCCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEEEECHHHHCHHHHHHHHHHHHHHCCCC
AMVLTDTGNVDSGVPSITVALRGLVVLEVEVRALEQSVHSGMWGGPVPDPTMALAKMLAA
EEEEEECCCCCCCCCHHHHHHCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LVDDDGAIAIPGIHDRVRPLTREQREAIAALPVTEEDIRRQARLRPGVRLLGGRHPLETN
HHCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEECCCCCCCCCC
WWQPSLAVNAIQASSRKDARNIINDVAWARLGVRLVPDMDPVDVREKLVAALHAAAPWGV
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHCCCCCE
EVTVKPDTAGAPWITDLGHPAFDAAFRALEKGFGRPALAIGCGGSIGFVEPFAKALGGVP
EEEECCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCC
ALLIGVEDPSSNAHSENESLLLSDFQKAIRSAIHLYEELAGALRR
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PETPVDLALAHYEKNAANYLDELKRLVRIPSVSFPGFPENEVRRSAEATAELLRRRGFE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
KVEVLEVEGAHPYVFGERVEDPAAPTLLLYAHHDVQPAGEAEAWKTPPFEPTERDGRLWG
EEEEEEECCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEC
RGAADDKAGIIVHAAAVDSWVRGARRMPLNVKIVVEGEEEIGSDHLTAFLTRYRSRLDAD
CCCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEEEECHHHHCHHHHHHHHHHHHHHCCCC
AMVLTDTGNVDSGVPSITVALRGLVVLEVEVRALEQSVHSGMWGGPVPDPTMALAKMLAA
EEEEEECCCCCCCCCHHHHHHCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LVDDDGAIAIPGIHDRVRPLTREQREAIAALPVTEEDIRRQARLRPGVRLLGGRHPLETN
HHCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEECCCCCCCCCC
WWQPSLAVNAIQASSRKDARNIINDVAWARLGVRLVPDMDPVDVREKLVAALHAAAPWGV
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHCCCCCE
EVTVKPDTAGAPWITDLGHPAFDAAFRALEKGFGRPALAIGCGGSIGFVEPFAKALGGVP
EEEECCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCC
ALLIGVEDPSSNAHSENESLLLSDFQKAIRSAIHLYEELAGALRR
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA