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The map label for this gene is sucB [H]

Identifier: 220915948

GI number: 220915948

Start: 934567

End: 935880

Strand: Direct

Name: sucB [H]

Synonym: A2cp1_0834

Alternate gene names: 220915948

Gene position: 934567-935880 (Clockwise)

Preceding gene: 220915947

Following gene: 220915954

Centisome position: 18.58

GC content: 74.2

Gene sequence:

>1314_bases
ATGTCCATCCAGCTCAAGGTCCCGAACATCGGCGAATCCGTGCAGACGGCCACCATCGGCACGTGGCTCAAGAAGGAGGG
CGAGCCGGTCCAGGCCGACGAGCCCGTGGTGGAGGTGGAGAGCGAGAAGGCCACCGTGGCGGTCCCGGCGCCCGCCGCCG
GCGTGCTGCGGAAGGTGCTCCGGCAGTCGGGCGAGACCGTCGCCATCGGCGAGGTGATCGCCGAGCTGGACGAGGGCGGC
GCCGCCCAGGCCGCGGGGGCCTCGCCGTTCGGCTCCGGCCCGGTGCCCGGCGGCACCGTCCCCGCCGCCCGCGGCGACGG
CGCCCCGGCGCCGGCCCCGGCTCCCGCCATCGCACCTGTCGCTTCCGCACCGGCCCGGGCCGCCGCCGCGGCGCCCGCGC
CCGCCGCGCCGCCGGCGGCCCCGCCCGCGCCTGCCGCCGCCGCCGGGTTCCGAGCGTCGCCCTCGGCGCGCCGCAGGATG
GCCGAGCTGGGCGTGACGGCCGGGCAGGTGGAGGCGATCGCCACCGGCGGCCAGATCCGCAGGGACGACGTGGCCCGCGC
GCTCGAGGCGCGCCCGGCCGCCCCCGCACCCGCGGCCCCGGCCGTGGCTGGCCCGCGCGAGCGCGTGGTCGCGATGACCC
CCCTGCGCCGCACCGTGGCGCGCCGGCTGGTCGAGGCGCAGCACACCGCGGCGCTGCTCACCACCTTCAACGAGGTGGAC
ATGTCGCGCGTGCTCGCGCTCCGCGAGCAGCACGGCGAGGCGTTCCTGAAGCGCCACGGCGTGAAGCTCGGCTTCATGTC
GTTCTTCGTGAAGGCGTCCATCGAGGCGCTGCGCGCCTACCCGGGCGTGAACGGCGAGATCCGCGGCGACAGCATCGTCT
ACAAGGACCACTACGACGTCGGCGTGGCGGTGGGCGGCGGCAAGGGCCTGGTGGTCCCGGTGATCCGCGACGCGGACGCG
CTCTCCTTCGCCGAGGTCGAGGCGACCATCGGCGAGCTGGCGAAGAAGGCGAAGGAGAACCGCATCACCATGGAGGACCT
CGCCGGCGGCACGTTCACCATCTCGAACGGCGGCATCTACGGTTCGATGCTCTCGACGCCCATCATCAACCCGCCGCAGT
CGGGCATCCTCGGCCTGCACAAGATCCAGAAGCGCGCGGTGGTGGACGCCGACGACCAGGTGGTGGTCCGCCCGATGATG
TACCTGGCGCTCTCGTACGATCACCGGCTGGTGGACGGCCGCGAGGCGGTCTCCTTCCTCGTGAAGGTGAAGGAGTGCAT
CGAGGATCCGGAGCGGATGCTGCTGGAGGTCTGA

Upstream 100 bases:

>100_bases
GCAGCTCGTGGCCGAGGCGCTCGGGGACCCCGTCCCCCGCATCCACCGCACCGACACGCGCGCCGCCGCGCACGAGCACT
GATCCACCCGGAAGAACACC

Downstream 100 bases:

>100_bases
CGCGGCGCGCCGGGCTTCACCCACCGTAGAGCCCGGCCTCCGGCGCGGGGCGGCCCTGGCCGACGAGGTCGAGCAGCGCG
AGCGCGTCGCGCACGGCGTC

Product: 2-oxoglutarate dehydrogenase, E2 subunit, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSIQLKVPNIGESVQTATIGTWLKKEGEPVQADEPVVEVESEKATVAVPAPAAGVLRKVLRQSGETVAIGEVIAELDEGG
AAQAAGASPFGSGPVPGGTVPAARGDGAPAPAPAPAIAPVASAPARAAAAAPAPAAPPAAPPAPAAAAGFRASPSARRRM
AELGVTAGQVEAIATGGQIRRDDVARALEARPAAPAPAAPAVAGPRERVVAMTPLRRTVARRLVEAQHTAALLTTFNEVD
MSRVLALREQHGEAFLKRHGVKLGFMSFFVKASIEALRAYPGVNGEIRGDSIVYKDHYDVGVAVGGGKGLVVPVIRDADA
LSFAEVEATIGELAKKAKENRITMEDLAGGTFTISNGGIYGSMLSTPIINPPQSGILGLHKIQKRAVVDADDQVVVRPMM
YLALSYDHRLVDGREAVSFLVKVKECIEDPERMLLEV

Sequences:

>Translated_437_residues
MSIQLKVPNIGESVQTATIGTWLKKEGEPVQADEPVVEVESEKATVAVPAPAAGVLRKVLRQSGETVAIGEVIAELDEGG
AAQAAGASPFGSGPVPGGTVPAARGDGAPAPAPAPAIAPVASAPARAAAAAPAPAAPPAAPPAPAAAAGFRASPSARRRM
AELGVTAGQVEAIATGGQIRRDDVARALEARPAAPAPAAPAVAGPRERVVAMTPLRRTVARRLVEAQHTAALLTTFNEVD
MSRVLALREQHGEAFLKRHGVKLGFMSFFVKASIEALRAYPGVNGEIRGDSIVYKDHYDVGVAVGGGKGLVVPVIRDADA
LSFAEVEATIGELAKKAKENRITMEDLAGGTFTISNGGIYGSMLSTPIINPPQSGILGLHKIQKRAVVDADDQVVVRPMM
YLALSYDHRLVDGREAVSFLVKVKECIEDPERMLLEV
>Mature_436_residues
SIQLKVPNIGESVQTATIGTWLKKEGEPVQADEPVVEVESEKATVAVPAPAAGVLRKVLRQSGETVAIGEVIAELDEGGA
AQAAGASPFGSGPVPGGTVPAARGDGAPAPAPAPAIAPVASAPARAAAAAPAPAAPPAAPPAPAAAAGFRASPSARRRMA
ELGVTAGQVEAIATGGQIRRDDVARALEARPAAPAPAAPAVAGPRERVVAMTPLRRTVARRLVEAQHTAALLTTFNEVDM
SRVLALREQHGEAFLKRHGVKLGFMSFFVKASIEALRAYPGVNGEIRGDSIVYKDHYDVGVAVGGGKGLVVPVIRDADAL
SFAEVEATIGELAKKAKENRITMEDLAGGTFTISNGGIYGSMLSTPIINPPQSGILGLHKIQKRAVVDADDQVVVRPMMY
LALSYDHRLVDGREAVSFLVKVKECIEDPERMLLEV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=228, Percent_Identity=56.578947368421, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI203098816, Length=472, Percent_Identity=25.635593220339, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI203098753, Length=472, Percent_Identity=25.635593220339, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI31711992, Length=444, Percent_Identity=28.1531531531532, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI110671329, Length=459, Percent_Identity=23.5294117647059, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI260898739, Length=157, Percent_Identity=33.1210191082803, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1786946, Length=443, Percent_Identity=44.6952595936795, Blast_Score=355, Evalue=4e-99,
Organism=Escherichia coli, GI1786305, Length=308, Percent_Identity=29.5454545454545, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25146366, Length=442, Percent_Identity=38.4615384615385, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17560088, Length=436, Percent_Identity=32.3394495412844, Blast_Score=191, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=454, Percent_Identity=25.1101321585903, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=228, Percent_Identity=31.140350877193, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=435, Percent_Identity=39.0804597701149, Blast_Score=294, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6324258, Length=469, Percent_Identity=25.3731343283582, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24645909, Length=237, Percent_Identity=52.3206751054852, Blast_Score=258, Evalue=5e-69,
Organism=Drosophila melanogaster, GI18859875, Length=454, Percent_Identity=24.8898678414097, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24582497, Length=429, Percent_Identity=31.7016317016317, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI20129315, Length=226, Percent_Identity=30.5309734513274, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45213; Mature: 45082

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQLKVPNIGESVQTATIGTWLKKEGEPVQADEPVVEVESEKATVAVPAPAAGVLRKVL
CEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEECCCHHHHHHHHH
RQSGETVAIGEVIAELDEGGAAQAAGASPFGSGPVPGGTVPAARGDGAPAPAPAPAIAPV
HCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ASAPARAAAAAPAPAAPPAAPPAPAAAAGFRASPSARRRMAELGVTAGQVEAIATGGQIR
CCCCCHHHCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCC
RDDVARALEARPAAPAPAAPAVAGPRERVVAMTPLRRTVARRLVEAQHTAALLTTFNEVD
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSRVLALREQHGEAFLKRHGVKLGFMSFFVKASIEALRAYPGVNGEIRGDSIVYKDHYDV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEECCCCE
GVAVGGGKGLVVPVIRDADALSFAEVEATIGELAKKAKENRITMEDLAGGTFTISNGGIY
EEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEECCCEE
GSMLSTPIINPPQSGILGLHKIQKRAVVDADDQVVVRPMMYLALSYDHRLVDGREAVSFL
HHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHH
VKVKECIEDPERMLLEV
HHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
SIQLKVPNIGESVQTATIGTWLKKEGEPVQADEPVVEVESEKATVAVPAPAAGVLRKVL
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEECCCHHHHHHHHH
RQSGETVAIGEVIAELDEGGAAQAAGASPFGSGPVPGGTVPAARGDGAPAPAPAPAIAPV
HCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ASAPARAAAAAPAPAAPPAAPPAPAAAAGFRASPSARRRMAELGVTAGQVEAIATGGQIR
CCCCCHHHCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCC
RDDVARALEARPAAPAPAAPAVAGPRERVVAMTPLRRTVARRLVEAQHTAALLTTFNEVD
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSRVLALREQHGEAFLKRHGVKLGFMSFFVKASIEALRAYPGVNGEIRGDSIVYKDHYDV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEECCCCE
GVAVGGGKGLVVPVIRDADALSFAEVEATIGELAKKAKENRITMEDLAGGTFTISNGGIY
EEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEECCCEE
GSMLSTPIINPPQSGILGLHKIQKRAVVDADDQVVVRPMMYLALSYDHRLVDGREAVSFL
HHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHH
VKVKECIEDPERMLLEV
HHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA