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The map label for this gene is btuB [H]

Identifier: 220915482

GI number: 220915482

Start: 406018

End: 407967

Strand: Direct

Name: btuB [H]

Synonym: A2cp1_0363

Alternate gene names: 220915482

Gene position: 406018-407967 (Clockwise)

Preceding gene: 220915481

Following gene: 220915483

Centisome position: 8.07

GC content: 77.54

Gene sequence:

>1950_bases
ATGAGCGCGTCGGCGCGGTTGGCCGCCCGGACGGCGATCGCGCTCGCGTGCGCGACGTGCCCGGCGCTGGCGGCCGGCGC
GGCGCCGGGACCGGATCCGGACGACCCGGTGGTGGTGCTGGAGGAGCAGGTGGTCCGCCTGCCGCGCGCAACGACGGAGG
CGCGGCCGCTGGCGTCCGGCACGGTGGTGGACCGCGCGACCTTCGAGGGCGAGGCGAAGGACGTCGGCCAGCTCGTCTCC
ACCGCGCCCGGGATCGCCGTGGAGGAGCACGGCGGCCTGGGCCAGCTCACCACGATCTCCATCCGCGGCTCCCCGCCGAG
CGGCGTCAAGGTGCTGCTGGATGGCTTGCCGCTGAACACCGCGTTCGGCGGCGGCGTGGACCTCTCCACCATCCCGCGCT
CCTGGGTGGACGCGGTGGAGGTGGTGCGAGGGCCGGAGGGAGCGCGCTACGGCTCGGGCGCGCTCGGCGGCGTGCTGAAC
GTGGTCACGCGCCGACCCGAGGCGGGGAGCTGGAGCCTCGAGACCGGCGGCGGCTCCTTCGACACGGCGCGGGTCGCGGC
CGACGTCGGCACCTCGGTCGGGCCAGGCGCGGTGTTGCTGGGCGCCACCGCCGAGACCACCAGCGGTCGGTTCCCGTTCC
GGCTCGATCCGGGTCTACCGGGTGGTCCCACGGTGCGGGCGACGCGAGAGAACGCCGGCGTGCGGCGCGGAGGCCTGCTC
GCGAAGCTGATCCGGCCGCAGGAGCACGGCCGGCTCGACGCGCTCCTGGAGGTGTCCGGCGGGCACCGCGGCCTCCCCGG
CTTCGCCGCGGCGCCGACCCCGTGGGCCTGGCAGGACGACGCGCGGCTGCTCGCGATGGGGCGGGTCGCGCGTTTCGGTG
CGCTCGGCCCGGCCACCCTCGCGGCCCGCGGCCACCTGCGGCTCGACCGGCTGGATGCGCGCCTCACCGGCCCCGACGAC
TCGCCGATCCGTCAGCGCGGCGCGGCGGCCGGGCTGGCGCTGGAGGCGACCGGGTCGCATCGGCACGGCGTCCTCAGCGC
CTCGCTCGAGACCACTGCCGAGCTCCTCGCCGCAGACGGGCTGGGGGGCCGCCGCGACCTCTATGGCGTGGCGGCGGCGG
TCGCCGACGACCTCAGCCTCGCCGGCGGCGGTCTCCGCATCGCCCCCGCCGCGCGAGCGGAGCGGGCCGGGCAGTTCGGC
GGCGTCTCCGGCTCGCTCGGCCTGTCGGTTCGGCTCGCCCCCGCCCTGCGCGCCCGCGCGGGCGTGGGCCGCACCTTCCG
GCCGCCCGGCCTCGCGGAGCTGCACCTGCAGCAGGGGCTCCTGGTCCCGAACCCGGACCTCCGGCCCGAGACCGCCGTCG
CGGCCGACGCGGGGTGGGTGGCGGACGGCCGCGCGGGGCTGCTCGCGGTCACCGGGCACCTCACCCGCTACGAGGACCTG
GTCGTGTACGAGCCGAGCGATGCGTTCGGTCGGCTGAAGCCCTTCAACTCCGGGCGCGCGTCGGCCACCGGCCTCGAGGT
CGAGGGCGCGTCCGCGCCGGTCGGCGGTCCGCTCCGCGCGAGCGCCCGCGGCAGCTACACCCTGCTCGTCACCGAGCTGC
TGCGCGGCGCGCCCGCGGAGGTGGGGCACTGGATCCCCTACCGCCCGAGGCAGCGCGCCTTCCTGCGCGTCGCGCTCGCC
CCCGGGCCGCTGTCGGCGCACGTGGAGCTCCACCGCCTCGGCCGTCGCTACCGGGATCGCCGCGGCGTGAACCCGATTCC
CTCCTCGACGAGCTGGAACGCCGGCCTCGGCGTGCGCGTGGCGCGCCGCCCCGGCGTCCGGATCCACCTCGAGGTCCTCA
ACCTCGCCGACGATCGGACGCTCACCGACGGCGTCGGCGATCCGCTCCCGCCGCGGACCGTGATGGTCACGCTGCGCGCC
GGGTCCTCTGCCCAGGAGTACGCACCATGA

Upstream 100 bases:

>100_bases
CGCACGTGTGCCCGCGTGGCCTCGCGCCGCAGCGGGAGGTCGAGCGCTGGCGCGCCGAGGCCGAGCGGGTGGCCTCGCGC
CACGCCGCGGAGGATCCCGC

Downstream 100 bases:

>100_bases
GACCGACGTTCCTCGCCACGCTCGCCGCCGCCGCGCTCCTCGCGTGCAGCGCACGGAGCTCCTGTCCCGAGGGGCAGGTC
GACTGCGGGGGGCGCTGCGT

Product: TonB-dependent receptor plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 649; Mature: 648

Protein sequence:

>649_residues
MSASARLAARTAIALACATCPALAAGAAPGPDPDDPVVVLEEQVVRLPRATTEARPLASGTVVDRATFEGEAKDVGQLVS
TAPGIAVEEHGGLGQLTTISIRGSPPSGVKVLLDGLPLNTAFGGGVDLSTIPRSWVDAVEVVRGPEGARYGSGALGGVLN
VVTRRPEAGSWSLETGGGSFDTARVAADVGTSVGPGAVLLGATAETTSGRFPFRLDPGLPGGPTVRATRENAGVRRGGLL
AKLIRPQEHGRLDALLEVSGGHRGLPGFAAAPTPWAWQDDARLLAMGRVARFGALGPATLAARGHLRLDRLDARLTGPDD
SPIRQRGAAAGLALEATGSHRHGVLSASLETTAELLAADGLGGRRDLYGVAAAVADDLSLAGGGLRIAPAARAERAGQFG
GVSGSLGLSVRLAPALRARAGVGRTFRPPGLAELHLQQGLLVPNPDLRPETAVAADAGWVADGRAGLLAVTGHLTRYEDL
VVYEPSDAFGRLKPFNSGRASATGLEVEGASAPVGGPLRASARGSYTLLVTELLRGAPAEVGHWIPYRPRQRAFLRVALA
PGPLSAHVELHRLGRRYRDRRGVNPIPSSTSWNAGLGVRVARRPGVRIHLEVLNLADDRTLTDGVGDPLPPRTVMVTLRA
GSSAQEYAP

Sequences:

>Translated_649_residues
MSASARLAARTAIALACATCPALAAGAAPGPDPDDPVVVLEEQVVRLPRATTEARPLASGTVVDRATFEGEAKDVGQLVS
TAPGIAVEEHGGLGQLTTISIRGSPPSGVKVLLDGLPLNTAFGGGVDLSTIPRSWVDAVEVVRGPEGARYGSGALGGVLN
VVTRRPEAGSWSLETGGGSFDTARVAADVGTSVGPGAVLLGATAETTSGRFPFRLDPGLPGGPTVRATRENAGVRRGGLL
AKLIRPQEHGRLDALLEVSGGHRGLPGFAAAPTPWAWQDDARLLAMGRVARFGALGPATLAARGHLRLDRLDARLTGPDD
SPIRQRGAAAGLALEATGSHRHGVLSASLETTAELLAADGLGGRRDLYGVAAAVADDLSLAGGGLRIAPAARAERAGQFG
GVSGSLGLSVRLAPALRARAGVGRTFRPPGLAELHLQQGLLVPNPDLRPETAVAADAGWVADGRAGLLAVTGHLTRYEDL
VVYEPSDAFGRLKPFNSGRASATGLEVEGASAPVGGPLRASARGSYTLLVTELLRGAPAEVGHWIPYRPRQRAFLRVALA
PGPLSAHVELHRLGRRYRDRRGVNPIPSSTSWNAGLGVRVARRPGVRIHLEVLNLADDRTLTDGVGDPLPPRTVMVTLRA
GSSAQEYAP
>Mature_648_residues
SASARLAARTAIALACATCPALAAGAAPGPDPDDPVVVLEEQVVRLPRATTEARPLASGTVVDRATFEGEAKDVGQLVST
APGIAVEEHGGLGQLTTISIRGSPPSGVKVLLDGLPLNTAFGGGVDLSTIPRSWVDAVEVVRGPEGARYGSGALGGVLNV
VTRRPEAGSWSLETGGGSFDTARVAADVGTSVGPGAVLLGATAETTSGRFPFRLDPGLPGGPTVRATRENAGVRRGGLLA
KLIRPQEHGRLDALLEVSGGHRGLPGFAAAPTPWAWQDDARLLAMGRVARFGALGPATLAARGHLRLDRLDARLTGPDDS
PIRQRGAAAGLALEATGSHRHGVLSASLETTAELLAADGLGGRRDLYGVAAAVADDLSLAGGGLRIAPAARAERAGQFGG
VSGSLGLSVRLAPALRARAGVGRTFRPPGLAELHLQQGLLVPNPDLRPETAVAADAGWVADGRAGLLAVTGHLTRYEDLV
VYEPSDAFGRLKPFNSGRASATGLEVEGASAPVGGPLRASARGSYTLLVTELLRGAPAEVGHWIPYRPRQRAFLRVALAP
GPLSAHVELHRLGRRYRDRRGVNPIPSSTSWNAGLGVRVARRPGVRIHLEVLNLADDRTLTDGVGDPLPPRTVMVTLRAG
SSAQEYAP

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=582, Percent_Identity=25.9450171821306, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1786798, Length=169, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 66797; Mature: 66665

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASARLAARTAIALACATCPALAAGAAPGPDPDDPVVVLEEQVVRLPRATTEARPLASG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCC
TVVDRATFEGEAKDVGQLVSTAPGIAVEEHGGLGQLTTISIRGSPPSGVKVLLDGLPLNT
CEEECEECCCCHHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCCC
AFGGGVDLSTIPRSWVDAVEVVRGPEGARYGSGALGGVLNVVTRRPEAGSWSLETGGGSF
CCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCC
DTARVAADVGTSVGPGAVLLGATAETTSGRFPFRLDPGLPGGPTVRATRENAGVRRGGLL
CHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCEEEEECCCCCCCCCCHH
AKLIRPQEHGRLDALLEVSGGHRGLPGFAAAPTPWAWQDDARLLAMGRVARFGALGPATL
HHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCHHH
AARGHLRLDRLDARLTGPDDSPIRQRGAAAGLALEATGSHRHGVLSASLETTAELLAADG
HHCCCEEEEECCCEECCCCCCHHHHHCCCCCEEEEECCCCCCCEEECHHHHHHHHHHHCC
LGGRRDLYGVAAAVADDLSLAGGGLRIAPAARAERAGQFGGVSGSLGLSVRLAPALRARA
CCCCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCCEEEEECHHHHHHH
GVGRTFRPPGLAELHLQQGLLVPNPDLRPETAVAADAGWVADGRAGLLAVTGHLTRYEDL
CCCCCCCCCCCHHHHHHCCEECCCCCCCCCCEEECCCCEEECCCCCEEEEECCCCCCCEE
VVYEPSDAFGRLKPFNSGRASATGLEVEGASAPVGGPLRASARGSYTLLVTELLRGAPAE
EEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEHHHHHCCCCHH
VGHWIPYRPRQRAFLRVALAPGPLSAHVELHRLGRRYRDRRGVNPIPSSTSWNAGLGVRV
HCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
ARRPGVRIHLEVLNLADDRTLTDGVGDPLPPRTVMVTLRAGSSAQEYAP
ECCCCCEEEEEEEECCCCCEECCCCCCCCCCCEEEEEEECCCCHHHCCC
>Mature Secondary Structure 
SASARLAARTAIALACATCPALAAGAAPGPDPDDPVVVLEEQVVRLPRATTEARPLASG
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCC
TVVDRATFEGEAKDVGQLVSTAPGIAVEEHGGLGQLTTISIRGSPPSGVKVLLDGLPLNT
CEEECEECCCCHHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCCC
AFGGGVDLSTIPRSWVDAVEVVRGPEGARYGSGALGGVLNVVTRRPEAGSWSLETGGGSF
CCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCC
DTARVAADVGTSVGPGAVLLGATAETTSGRFPFRLDPGLPGGPTVRATRENAGVRRGGLL
CHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCEEEEECCCCCCCCCCHH
AKLIRPQEHGRLDALLEVSGGHRGLPGFAAAPTPWAWQDDARLLAMGRVARFGALGPATL
HHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCHHH
AARGHLRLDRLDARLTGPDDSPIRQRGAAAGLALEATGSHRHGVLSASLETTAELLAADG
HHCCCEEEEECCCEECCCCCCHHHHHCCCCCEEEEECCCCCCCEEECHHHHHHHHHHHCC
LGGRRDLYGVAAAVADDLSLAGGGLRIAPAARAERAGQFGGVSGSLGLSVRLAPALRARA
CCCCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCCEEEEECHHHHHHH
GVGRTFRPPGLAELHLQQGLLVPNPDLRPETAVAADAGWVADGRAGLLAVTGHLTRYEDL
CCCCCCCCCCCHHHHHHCCEECCCCCCCCCCEEECCCCEEECCCCCEEEEECCCCCCCEE
VVYEPSDAFGRLKPFNSGRASATGLEVEGASAPVGGPLRASARGSYTLLVTELLRGAPAE
EEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEHHHHHCCCCHH
VGHWIPYRPRQRAFLRVALAPGPLSAHVELHRLGRRYRDRRGVNPIPSSTSWNAGLGVRV
HCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
ARRPGVRIHLEVLNLADDRTLTDGVGDPLPPRTVMVTLRAGSSAQEYAP
ECCCCCEEEEEEEECCCCCEECCCCCCCCCCCEEEEEEECCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]