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The map label for this gene is rsmE [H]

Identifier: 220915389

GI number: 220915389

Start: 299676

End: 300398

Strand: Direct

Name: rsmE [H]

Synonym: A2cp1_0268

Alternate gene names: 220915389

Gene position: 299676-300398 (Clockwise)

Preceding gene: 220915388

Following gene: 220915390

Centisome position: 5.96

GC content: 81.05

Gene sequence:

>723_bases
GTGAGCCTGCGCCGGGTCCACCTCCCGCCGGAGCGGATCGGCGAGGGCCGCGCCGGGCTCACCGACGAGGCGCGCCACTA
CCTGCGCGACGTGCTCCGGCTCGCGCCGGGCGCGGCGGTGGAGCTGTTCGATGGCCGGGGCGGCGCCTGGGAGGCCACCG
TCCTCGACGGCTTCGAGGCGCTCGCGCTCGGCGCCCGCCGCGCGGCGCCGGGGGGCGGCGCGCCGGTGTGGCTGCTCGTG
GCGCTCGCCAAGGGCGAGAAGATCGACCTCGTGGTGCAGAAGGCGACCGAGCTGGGCGCGGCGCGGATCGCGCCGTTCGC
GGCGGAGCGCTCGGTGGTGCGGCTCGAGCCGGAGAAGGGCGAGGCGCGCGCGGCGCGCTGGCGGCGGATCGCGGAGGAGG
CGGCCCGCCAGTGCGGGCGCGCCGACGTGCCGGAGGTGCGGGCGCCGGCGCCGCTCGGCGCGGCGCTCGCCGAGGTGCCG
GCCGGCTTCGGCGCGTTCGTGTTCCACCCCGGCGGCGCGGCGCTGTCCGAGGCCGCGCCCTCGCCGGCGGGCGGCTACGC
CGCGGTGGTCGGCCCCGAGGGCGGGCTCACCGACGCCGAGCGCGAGGCCTGCGCGCGCGCCGGCGCCCGGGACACCTCGC
TCGGGCCGCGCGTGCTCCGCGCCGAGACCGCCGCCATCGTCGCGGTCGCGCTGCTGCAGGCGCGCTTCGGCGACCTCCGT
TGA

Upstream 100 bases:

>100_bases
AGGTGCGCGCGGCCTACCTGGCGCTGGGGCTCCGCCCGCTCGCGGGCGGCGACCGGCGCGAGGGCGAGTGGAGCCTGCTC
GCGCTGGAGAAGCCGCGCGC

Downstream 100 bases:

>100_bases
GGTCGCGGGCATCCCGCCCATTCCTTGACCCCCACCGGCGCCGACCGTAGCCTCGGGCCGTGCGATGCCCGAGGTGCCAC
GGCGAGGCCGGCCAGGGGAT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MSLRRVHLPPERIGEGRAGLTDEARHYLRDVLRLAPGAAVELFDGRGGAWEATVLDGFEALALGARRAAPGGGAPVWLLV
ALAKGEKIDLVVQKATELGAARIAPFAAERSVVRLEPEKGEARAARWRRIAEEAARQCGRADVPEVRAPAPLGAALAEVP
AGFGAFVFHPGGAALSEAAPSPAGGYAAVVGPEGGLTDAEREACARAGARDTSLGPRVLRAETAAIVAVALLQARFGDLR

Sequences:

>Translated_240_residues
MSLRRVHLPPERIGEGRAGLTDEARHYLRDVLRLAPGAAVELFDGRGGAWEATVLDGFEALALGARRAAPGGGAPVWLLV
ALAKGEKIDLVVQKATELGAARIAPFAAERSVVRLEPEKGEARAARWRRIAEEAARQCGRADVPEVRAPAPLGAALAEVP
AGFGAFVFHPGGAALSEAAPSPAGGYAAVVGPEGGLTDAEREACARAGARDTSLGPRVLRAETAAIVAVALLQARFGDLR
>Mature_239_residues
SLRRVHLPPERIGEGRAGLTDEARHYLRDVLRLAPGAAVELFDGRGGAWEATVLDGFEALALGARRAAPGGGAPVWLLVA
LAKGEKIDLVVQKATELGAARIAPFAAERSVVRLEPEKGEARAARWRRIAEEAARQCGRADVPEVRAPAPLGAALAEVPA
GFGAFVFHPGGAALSEAAPSPAGGYAAVVGPEGGLTDAEREACARAGARDTSLGPRVLRAETAAIVAVALLQARFGDLR

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=34.156378600823, Blast_Score=142, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 24840; Mature: 24708

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRRVHLPPERIGEGRAGLTDEARHYLRDVLRLAPGAAVELFDGRGGAWEATVLDGFEA
CCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHH
LALGARRAAPGGGAPVWLLVALAKGEKIDLVVQKATELGAARIAPFAAERSVVRLEPEKG
HHHHHHHCCCCCCCHHHHHEEHHCCCEEEHHHHHHHHHCHHHHCHHHHCCCEEEECCCCC
EARAARWRRIAEEAARQCGRADVPEVRAPAPLGAALAEVPAGFGAFVFHPGGAALSEAAP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCHHHHCCC
SPAGGYAAVVGPEGGLTDAEREACARAGARDTSLGPRVLRAETAAIVAVALLQARFGDLR
CCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLRRVHLPPERIGEGRAGLTDEARHYLRDVLRLAPGAAVELFDGRGGAWEATVLDGFEA
CCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHH
LALGARRAAPGGGAPVWLLVALAKGEKIDLVVQKATELGAARIAPFAAERSVVRLEPEKG
HHHHHHHCCCCCCCHHHHHEEHHCCCEEEHHHHHHHHHCHHHHCHHHHCCCEEEECCCCC
EARAARWRRIAEEAARQCGRADVPEVRAPAPLGAALAEVPAGFGAFVFHPGGAALSEAAP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCHHHHCCC
SPAGGYAAVVGPEGGLTDAEREACARAGARDTSLGPRVLRAETAAIVAVALLQARFGDLR
CCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]