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The map label for this gene is rfbB [C]

Identifier: 220915364

GI number: 220915364

Start: 260936

End: 261910

Strand: Direct

Name: rfbB [C]

Synonym: A2cp1_0243

Alternate gene names: 220915364

Gene position: 260936-261910 (Clockwise)

Preceding gene: 220915362

Following gene: 220915365

Centisome position: 5.19

GC content: 70.36

Gene sequence:

>975_bases
ATGCCCCCGACCCTGGTCACCGGCGCCGCCGGCTTCATCGGATTCCACCTCGCGAAGGCGCTGCTCGACCGCGGCGACGT
GGTGCTCGGCGTCGACTCGATGGTGCCGTACTACGACGTCCGCCTGAAGGAGGCGCGCCTGGCCCAGCTCACCGGGCGCC
CCGGGTTCACGTTCGAGCGGCTGGATCTCGCCGATCGCGAGGCGACCTCCCGTCTGTTCGAGCGCGGCGGCTTCGGCGCG
GTCGTGCACCTCGCCGCCCAGCCCGGCGTGCGCTACTCGCTCGAGAACCCGCACGCCTACGTGGACGCGAACGTCACCGG
GTTCCTCGACGTGCTCGAGGGCTGCCGCCGGCACCCGGTCCGCCACCTGGTCTACGCGTCCTCGAGCTCCGTCTACGGCG
GCAACACCAAGGTGCCGTTCGCGGTCACCGACAACGTCGATCACCCGGTCAGCCTGTACGCCGCCACCAAGAAGGCGAAC
GAGCTGATGGCGTACACGTACAGCCACCTGTTCGCGATCCCCGCCACGGGGCTCCGCTTCTTCACCGTGTACGGTCCGTG
GGGACGTCCGGACATGGCGCCCATGCTCTTCGCCCGCGCCATCCTCGAGGGCCAGCCGATCAAGGTCTTCAACCACGGGC
AGATGCGGCGCGACTTCACCTACGTGGACGACATCGTCGAGGGCGTCATCCGCGTCCTCGACCGCCCGCCCGCGGCGGGC
GTGGTGCCGCCGCACCGGCTCTACAACATCGGCAACAGCCAGCCCGTCGAGCTGCTCCGCTTCATCGACGTGATGGAGGC
GGCGCTCGGGAAGAAGGCGGTCCGCGAGCTCCTGCCGATGCAGCCCGGCGACGTCCCGGCCACGTTCGCCGACGTGTCGG
ATCTGGAGCGGGACGTCGGCTTCCGGCCAGCGACCTCCATCGAGGAGGGCGTGCGACGCTTCGTGGCCTGGTACCGGGCG
TACCACCGCGCGTGA

Upstream 100 bases:

>100_bases
CCTTGGGCGAAGCCAATGGGCGGGGCGTGCCGTGGTCCGCGGATCGGACCCCTGGTCCGAAATGCACTACTCTGGGTCCG
GAACTGCGACAGGAACGTCC

Downstream 100 bases:

>100_bases
GCCGCCGGCGCGGCCCGGTCCCGGCCGGGCGCGCTCCCCACCCTCGCGGACGCCGGACCCGAACGCAGGGTCGCGCCGCG
CAGGCAGGCGGCCCACCGTG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MPPTLVTGAAGFIGFHLAKALLDRGDVVLGVDSMVPYYDVRLKEARLAQLTGRPGFTFERLDLADREATSRLFERGGFGA
VVHLAAQPGVRYSLENPHAYVDANVTGFLDVLEGCRRHPVRHLVYASSSSVYGGNTKVPFAVTDNVDHPVSLYAATKKAN
ELMAYTYSHLFAIPATGLRFFTVYGPWGRPDMAPMLFARAILEGQPIKVFNHGQMRRDFTYVDDIVEGVIRVLDRPPAAG
VVPPHRLYNIGNSQPVELLRFIDVMEAALGKKAVRELLPMQPGDVPATFADVSDLERDVGFRPATSIEEGVRRFVAWYRA
YHRA

Sequences:

>Translated_324_residues
MPPTLVTGAAGFIGFHLAKALLDRGDVVLGVDSMVPYYDVRLKEARLAQLTGRPGFTFERLDLADREATSRLFERGGFGA
VVHLAAQPGVRYSLENPHAYVDANVTGFLDVLEGCRRHPVRHLVYASSSSVYGGNTKVPFAVTDNVDHPVSLYAATKKAN
ELMAYTYSHLFAIPATGLRFFTVYGPWGRPDMAPMLFARAILEGQPIKVFNHGQMRRDFTYVDDIVEGVIRVLDRPPAAG
VVPPHRLYNIGNSQPVELLRFIDVMEAALGKKAVRELLPMQPGDVPATFADVSDLERDVGFRPATSIEEGVRRFVAWYRA
YHRA
>Mature_323_residues
PPTLVTGAAGFIGFHLAKALLDRGDVVLGVDSMVPYYDVRLKEARLAQLTGRPGFTFERLDLADREATSRLFERGGFGAV
VHLAAQPGVRYSLENPHAYVDANVTGFLDVLEGCRRHPVRHLVYASSSSVYGGNTKVPFAVTDNVDHPVSLYAATKKANE
LMAYTYSHLFAIPATGLRFFTVYGPWGRPDMAPMLFARAILEGQPIKVFNHGQMRRDFTYVDDIVEGVIRVLDRPPAAGV
VPPHRLYNIGNSQPVELLRFIDVMEAALGKKAVRELLPMQPGDVPATFADVSDLERDVGFRPATSIEEGVRRFVAWYRAY
HRA

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI56237023, Length=338, Percent_Identity=28.9940828402367, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI56118217, Length=338, Percent_Identity=28.9940828402367, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI189083684, Length=338, Percent_Identity=28.9940828402367, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI7657641, Length=323, Percent_Identity=25.3869969040248, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI42516563, Length=327, Percent_Identity=24.7706422018349, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1788353, Length=342, Percent_Identity=26.6081871345029, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI48994969, Length=349, Percent_Identity=27.7936962750716, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786974, Length=333, Percent_Identity=24.3243243243243, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790049, Length=334, Percent_Identity=25.4491017964072, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71982038, Length=340, Percent_Identity=26.7647058823529, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71982035, Length=340, Percent_Identity=27.0588235294118, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17568069, Length=335, Percent_Identity=22.9850746268657, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=323, Percent_Identity=23.8390092879257, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115532424, Length=338, Percent_Identity=22.189349112426, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=344, Percent_Identity=25.2906976744186, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19923002, Length=339, Percent_Identity=27.7286135693215, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21356223, Length=326, Percent_Identity=24.5398773006135, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 35874; Mature: 35742

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPTLVTGAAGFIGFHLAKALLDRGDVVLGVDSMVPYYDVRLKEARLAQLTGRPGFTFER
CCCCEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHEEHHHHHHHHCCCCCCEEHH
LDLADREATSRLFERGGFGAVVHLAAQPGVRYSLENPHAYVDANVTGFLDVLEGCRRHPV
HCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCCEEEECCHHHHHHHHHHHHHCCH
RHLVYASSSSVYGGNTKVPFAVTDNVDHPVSLYAATKKANELMAYTYSHLFAIPATGLRF
HEEEEECCCCCCCCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEE
FTVYGPWGRPDMAPMLFARAILEGQPIKVFNHGQMRRDFTYVDDIVEGVIRVLDRPPAAG
EEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
VVPPHRLYNIGNSQPVELLRFIDVMEAALGKKAVRELLPMQPGDVPATFADVSDLERDVG
CCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
FRPATSIEEGVRRFVAWYRAYHRA
CCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PPTLVTGAAGFIGFHLAKALLDRGDVVLGVDSMVPYYDVRLKEARLAQLTGRPGFTFER
CCCEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHEEHHHHHHHHCCCCCCEEHH
LDLADREATSRLFERGGFGAVVHLAAQPGVRYSLENPHAYVDANVTGFLDVLEGCRRHPV
HCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCCEEEECCHHHHHHHHHHHHHCCH
RHLVYASSSSVYGGNTKVPFAVTDNVDHPVSLYAATKKANELMAYTYSHLFAIPATGLRF
HEEEEECCCCCCCCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEE
FTVYGPWGRPDMAPMLFARAILEGQPIKVFNHGQMRRDFTYVDDIVEGVIRVLDRPPAAG
EEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
VVPPHRLYNIGNSQPVELLRFIDVMEAALGKKAVRELLPMQPGDVPATFADVSDLERDVG
CCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
FRPATSIEEGVRRFVAWYRAYHRA
CCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]