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The map label for this gene is ptsH [H]

Identifier: 220915291

GI number: 220915291

Start: 186590

End: 186862

Strand: Direct

Name: ptsH [H]

Synonym: A2cp1_0170

Alternate gene names: 220915291

Gene position: 186590-186862 (Clockwise)

Preceding gene: 220915290

Following gene: 220915292

Centisome position: 3.71

GC content: 67.4

Gene sequence:

>273_bases
ATGCCGCACCAGGAGCGCACGTTCGTCATCGTCAACACGCTCGGCCTCCACGCGCGCGCCGCGGCGCAGCTCGTCCAGAC
CTCGAACCGCTACCGGTCCGAGATCCACGTCGAGAAGGACGGCATGCAGGTGAACGGGAAGAGCATCATGGGCGTGCTCA
CGCTCGCCGCGGCCAAGGGCTCGCAGATCACCGTGACCTGCGACGGCGACGACGCCGACCAGGCCATGACCGCGCTCGCC
AAGGTGATCGAGAACGGGTTCGGGGAGACATGA

Upstream 100 bases:

>100_bases
TGGCCCGCGGCGCCCGCCTGCTCCCCACCGCCTACGTCGCCACCCTGGCCGGGGTCGGCGCCGCGCTCCTCCTCGGCCAC
CTCCACGGGAGCAGCTAGGG

Downstream 100 bases:

>100_bases
GCCGGTCGTCCGCCACCACGCTCGTCGGCATCGGCGCCTCGCCCGGCATCGCCATCGGGCGCTGCTGGACGATCGAGCGG
CGGCGGGTGCGGACCCCCAA

Product: phosphocarrier, HPr family

Products: fructose-6-phosphate [Cytoplasm]; pyruvate; fructose-1-phosphate [Cytoplasm] [C]

Alternate protein names: Histidine-containing protein [H]

Number of amino acids: Translated: 90; Mature: 89

Protein sequence:

>90_residues
MPHQERTFVIVNTLGLHARAAAQLVQTSNRYRSEIHVEKDGMQVNGKSIMGVLTLAAAKGSQITVTCDGDDADQAMTALA
KVIENGFGET

Sequences:

>Translated_90_residues
MPHQERTFVIVNTLGLHARAAAQLVQTSNRYRSEIHVEKDGMQVNGKSIMGVLTLAAAKGSQITVTCDGDDADQAMTALA
KVIENGFGET
>Mature_89_residues
PHQERTFVIVNTLGLHARAAAQLVQTSNRYRSEIHVEKDGMQVNGKSIMGVLTLAAAKGSQITVTCDGDDADQAMTALAK
VIENGFGET

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=85, Percent_Identity=40, Blast_Score=61, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 9629; Mature: 9498

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00369 PTS_HPR_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHQERTFVIVNTLGLHARAAAQLVQTSNRYRSEIHVEKDGMQVNGKSIMGVLTLAAAKG
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCEEECCHHHHHHHHHHCCCC
SQITVTCDGDDADQAMTALAKVIENGFGET
CEEEEEECCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PHQERTFVIVNTLGLHARAAAQLVQTSNRYRSEIHVEKDGMQVNGKSIMGVLTLAAAKG
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCEEECCHHHHHHHHHHCCCC
SQITVTCDGDDADQAMTALAKVIENGFGET
CEEEEEECCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Fructose [Periplasm]; phosphoenolpyruvate [C]

Specific reaction: phosphoenolpyruvate + fructose [Periplasm] = fructose-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + fructose [Periplasm] = fructose-1-phosphate [Cytoplasm] + pyruvate [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]