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The map label for this gene is thiE [H]

Identifier: 220915214

GI number: 220915214

Start: 103237

End: 103905

Strand: Direct

Name: thiE [H]

Synonym: A2cp1_0093

Alternate gene names: 220915214

Gene position: 103237-103905 (Clockwise)

Preceding gene: 220915210

Following gene: 220915215

Centisome position: 2.05

GC content: 80.12

Gene sequence:

>669_bases
ATGCCGGTTTCCGAAGCCTCGGTGGGCGCCGGGCGGCGCGCGCGGCTCGGCGGGCTCTACGTCATCGTGGGGGGGGCGGA
TCCGGTGGCGCAGGCACATGCCGCGATCGGCGGAGGCGCCCGCGCCGTCCAGGTGCGGATGAAGGACGCGCCCGCCGGCG
CGGTGCTCGAGGCCACCCGCCGCATCCTGGCGCTCGCCGCCGGCCGGGCGCTCGTGCTCGTGAACGACCGCGCCGACCTG
GCGCTCCTCGCCGGCGCCGACGGCGTCCACCTCGGCGACGACGACCTGCCGGTGCCGGAGGCGCGGCGGCTGCTCGGCCC
CGACCTGCTGGTGGGTCGCACCACCCGGACGCTGGAGGAGGCCCGGGCGGCGCTCGCCGAGGGAGCCGATCACGTGGGCT
ACGGGCCCATCTTCGCGTCCCGGTCGAAGGCGCTGCCGGTGCCGCCGCGCGGGCTGCAGGCGCTCGCCGAGGTGGCGCGC
GCGCTCCCCGCGCCGGTGGTGGCGATCGGCGGCATCGGGCTCGACGACGTCGGCGCGGTGGCGCGGGCGGGCGCCGCCTG
CGCGGCGGTGATCGAGGCGGTGGTGGGCGCGGCGGATCCGGAGGCCGCCGCGGCGCGCATGCAGGCCGCGTTCGAGGCCG
GCCGCGCCGCGCGGGGGGCGAACCCGTGA

Upstream 100 bases:

>100_bases
CTCGAACGGGTCGGCCATGTCACTTCCTTGCTGTCTGGGCGGAGGCCGGAAAATAGGCCCCGCCTTGCGGCCCCGTCAAG
CGCGTGTTAGCGCTGTCGCC

Downstream 100 bases:

>100_bases
GCCCGCGCCCGAGGATCGGGATCACGCTCGACGCCGACGCGTCGGGCCGCCGCTACGAGCTGCCGCGCGGCTACGTGGAG
GCGGTGCTGGACGCGGGCGG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MPVSEASVGAGRRARLGGLYVIVGGADPVAQAHAAIGGGARAVQVRMKDAPAGAVLEATRRILALAAGRALVLVNDRADL
ALLAGADGVHLGDDDLPVPEARRLLGPDLLVGRTTRTLEEARAALAEGADHVGYGPIFASRSKALPVPPRGLQALAEVAR
ALPAPVVAIGGIGLDDVGAVARAGAACAAVIEAVVGAADPEAAAARMQAAFEAGRAARGANP

Sequences:

>Translated_222_residues
MPVSEASVGAGRRARLGGLYVIVGGADPVAQAHAAIGGGARAVQVRMKDAPAGAVLEATRRILALAAGRALVLVNDRADL
ALLAGADGVHLGDDDLPVPEARRLLGPDLLVGRTTRTLEEARAALAEGADHVGYGPIFASRSKALPVPPRGLQALAEVAR
ALPAPVVAIGGIGLDDVGAVARAGAACAAVIEAVVGAADPEAAAARMQAAFEAGRAARGANP
>Mature_221_residues
PVSEASVGAGRRARLGGLYVIVGGADPVAQAHAAIGGGARAVQVRMKDAPAGAVLEATRRILALAAGRALVLVNDRADLA
LLAGADGVHLGDDDLPVPEARRLLGPDLLVGRTTRTLEEARAALAEGADHVGYGPIFASRSKALPVPPRGLQALAEVARA
LPAPVVAIGGIGLDDVGAVARAGAACAAVIEAVVGAADPEAAAARMQAAFEAGRAARGANP

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=195, Percent_Identity=35.3846153846154, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325042, Length=214, Percent_Identity=28.0373831775701, Blast_Score=82, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 21976; Mature: 21845

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVSEASVGAGRRARLGGLYVIVGGADPVAQAHAAIGGGARAVQVRMKDAPAGAVLEATR
CCCCCCCCCCCCCEECCCEEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
RILALAAGRALVLVNDRADLALLAGADGVHLGDDDLPVPEARRLLGPDLLVGRTTRTLEE
HHHHHHCCCEEEEEECCCCEEEEECCCCCEECCCCCCCHHHHHHCCCCCEECCHHHHHHH
ARAALAEGADHVGYGPIFASRSKALPVPPRGLQALAEVARALPAPVVAIGGIGLDDVGAV
HHHHHHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHH
ARAGAACAAVIEAVVGAADPEAAAARMQAAFEAGRAARGANP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PVSEASVGAGRRARLGGLYVIVGGADPVAQAHAAIGGGARAVQVRMKDAPAGAVLEATR
CCCCCCCCCCCCEECCCEEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
RILALAAGRALVLVNDRADLALLAGADGVHLGDDDLPVPEARRLLGPDLLVGRTTRTLEE
HHHHHHCCCEEEEEECCCCEEEEECCCCCEECCCCCCCHHHHHHCCCCCEECCHHHHHHH
ARAALAEGADHVGYGPIFASRSKALPVPPRGLQALAEVARALPAPVVAIGGIGLDDVGAV
HHHHHHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHH
ARAGAACAAVIEAVVGAADPEAAAARMQAAFEAGRAARGANP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA