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The map label for this gene is buk1 [H]

Identifier: 220915180

GI number: 220915180

Start: 65599

End: 66729

Strand: Direct

Name: buk1 [H]

Synonym: A2cp1_0058

Alternate gene names: 220915180

Gene position: 65599-66729 (Clockwise)

Preceding gene: 220915179

Following gene: 220915182

Centisome position: 1.3

GC content: 80.19

Gene sequence:

>1131_bases
GTGAGCGCGGGCGCGGGGCCTCCGGGGAGCGGCGCCGGCGCCGGCGGCGGGCCGCTGGTGCTGGCGGTGAACCCGGGCGC
CGGGTCCACCAAGCTCGGCCTGTTCCGCGGCGGCCTGCCATTGCGCGAGGAGAAGGTGCTCCACCCCGAGGCGATGGCGC
GCCCCGCAGCGCGGATCTGGGACGAGCTGCCCGGGCGCGTCGCCGCCGCCGAGGACTTCCTCTCGCGGGCCGGGGTGCGG
GCGGGGGAGCTCGCCGCGGTGGCCGGCCGGGGCGGGCTGCTCCCGCCGCTCGCCGCCGGCGCGTACCTGGTGGACGACGC
GCTGGTGGCCGAGCTGGAGCGGGCCGCGCACGGCGAGCACGCCTCCAACCTGGGCGCCCCCATGGCGCGGGCGCTGGCCG
CGCCGCACGGCTGCCCGGCGCTGGTGGTGGACCCGGTCTCGGTGGACGAGCTCGCGCCGGTGGCGCGCGTCACCGGCCTC
GCCGGGGTGGAGCGCTCCAGCTTCGTGCACGCGCTCAACCTCCGGGCGGTGGCGCGCCGCCACGCCGCCGGGGCGGGGCG
GCCGCTCGAGGCGCTCCGGCTGGTGCTCGCGCACCTCGGCACCGGGATCTCGCTCTGCGCCCTGTCCGGCGGCCGGATGG
TGGACGTGGTGAACCCGCGCGACGAGGGGCCGTTCTCGGGCGACCGGGCCGGCGGCGTGCCCGCGAACGCGCTCGTCGAC
CTGTGCTTCGCGCCGGGCGCCGACGTGCGGACGGTGAGGCGCCGGCTGTTCGGCGACGGCGGGCTCTACGCGCACCTCGG
CACGCGCGACGTCCGGGAGGCGCTGGCGCGCGCCGAGCGCGGGGACGCCCGGGCCGCGCTGCTGGTGGACGCCATGTGCT
ACCAGGCCGCGAAGGCCGTGGCGGCGATGGCCGTCGCGCTCGACGGGCGGGTGGACGCGATCGTGCTCACCGGCGGCCTC
GCGCACCTCGCGCCGGTGGTGGAGGGCGTCCGCCGCCGGGTGGCGTGGATCGCGCCGGTGGAGATCCATCCGGGCGAGGA
CGAGCTGCGCGCGCTCGCCGAGGGCGCGCTGCGCGTCCTCGCCGGCGAGGAGCCGGCCCGGCGCTACGCGCCGCTCGCTC
CGGCGCGCTGA

Upstream 100 bases:

>100_bases
GCGCCCGCGCGCCGGTGCTCATTCCGTCGCGGGTGGAGCGCCCGGAGGACAAGCTCTGCTCGATCGCGCTCGGCGTGCTG
GCGGCGGCGCGGGGGACCCG

Downstream 100 bases:

>100_bases
CCGCCGGCGCGCGGCGACGCGCCGCTCGCTTCGCCCTCCGACCTCACCGCGGCAGCGCCGCGGCCAGCCGGCGCACCGCC
TCGGCCAGCTCGCGCTCGTC

Product: butyrate kinase

Products: NA

Alternate protein names: BK 1; Branched-chain carboxylic acid kinase 1 [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSAGAGPPGSGAGAGGGPLVLAVNPGAGSTKLGLFRGGLPLREEKVLHPEAMARPAARIWDELPGRVAAAEDFLSRAGVR
AGELAAVAGRGGLLPPLAAGAYLVDDALVAELERAAHGEHASNLGAPMARALAAPHGCPALVVDPVSVDELAPVARVTGL
AGVERSSFVHALNLRAVARRHAAGAGRPLEALRLVLAHLGTGISLCALSGGRMVDVVNPRDEGPFSGDRAGGVPANALVD
LCFAPGADVRTVRRRLFGDGGLYAHLGTRDVREALARAERGDARAALLVDAMCYQAAKAVAAMAVALDGRVDAIVLTGGL
AHLAPVVEGVRRRVAWIAPVEIHPGEDELRALAEGALRVLAGEEPARRYAPLAPAR

Sequences:

>Translated_376_residues
MSAGAGPPGSGAGAGGGPLVLAVNPGAGSTKLGLFRGGLPLREEKVLHPEAMARPAARIWDELPGRVAAAEDFLSRAGVR
AGELAAVAGRGGLLPPLAAGAYLVDDALVAELERAAHGEHASNLGAPMARALAAPHGCPALVVDPVSVDELAPVARVTGL
AGVERSSFVHALNLRAVARRHAAGAGRPLEALRLVLAHLGTGISLCALSGGRMVDVVNPRDEGPFSGDRAGGVPANALVD
LCFAPGADVRTVRRRLFGDGGLYAHLGTRDVREALARAERGDARAALLVDAMCYQAAKAVAAMAVALDGRVDAIVLTGGL
AHLAPVVEGVRRRVAWIAPVEIHPGEDELRALAEGALRVLAGEEPARRYAPLAPAR
>Mature_375_residues
SAGAGPPGSGAGAGGGPLVLAVNPGAGSTKLGLFRGGLPLREEKVLHPEAMARPAARIWDELPGRVAAAEDFLSRAGVRA
GELAAVAGRGGLLPPLAAGAYLVDDALVAELERAAHGEHASNLGAPMARALAAPHGCPALVVDPVSVDELAPVARVTGLA
GVERSSFVHALNLRAVARRHAAGAGRPLEALRLVLAHLGTGISLCALSGGRMVDVVNPRDEGPFSGDRAGGVPANALVDL
CFAPGADVRTVRRRLFGDGGLYAHLGTRDVREALARAERGDARAALLVDAMCYQAAKAVAAMAVALDGRVDAIVLTGGLA
HLAPVVEGVRRRVAWIAPVEIHPGEDELRALAEGALRVLAGEEPARRYAPLAPAR

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=166, Percent_Identity=30.1204819277108, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 38281; Mature: 38150

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGAGPPGSGAGAGGGPLVLAVNPGAGSTKLGLFRGGLPLREEKVLHPEAMARPAARIW
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHCCCHHHHHCHHHHHH
DELPGRVAAAEDFLSRAGVRAGELAAVAGRGGLLPPLAAGAYLVDDALVAELERAAHGEH
HHCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCC
ASNLGAPMARALAAPHGCPALVVDPVSVDELAPVARVTGLAGVERSSFVHALNLRAVARR
HHHCCHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
HAAGAGRPLEALRLVLAHLGTGISLCALSGGRMVDVVNPRDEGPFSGDRAGGVPANALVD
HHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHH
LCFAPGADVRTVRRRLFGDGGLYAHLGTRDVREALARAERGDARAALLVDAMCYQAAKAV
HHHCCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AAMAVALDGRVDAIVLTGGLAHLAPVVEGVRRRVAWIAPVEIHPGEDELRALAEGALRVL
HHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHH
AGEEPARRYAPLAPAR
CCCCHHHHCCCCCCCC
>Mature Secondary Structure 
SAGAGPPGSGAGAGGGPLVLAVNPGAGSTKLGLFRGGLPLREEKVLHPEAMARPAARIW
CCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHCCCHHHHHCHHHHHH
DELPGRVAAAEDFLSRAGVRAGELAAVAGRGGLLPPLAAGAYLVDDALVAELERAAHGEH
HHCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCC
ASNLGAPMARALAAPHGCPALVVDPVSVDELAPVARVTGLAGVERSSFVHALNLRAVARR
HHHCCHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
HAAGAGRPLEALRLVLAHLGTGISLCALSGGRMVDVVNPRDEGPFSGDRAGGVPANALVD
HHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHH
LCFAPGADVRTVRRRLFGDGGLYAHLGTRDVREALARAERGDARAALLVDAMCYQAAKAV
HHHCCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AAMAVALDGRVDAIVLTGGLAHLAPVVEGVRRRVAWIAPVEIHPGEDELRALAEGALRVL
HHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHH
AGEEPARRYAPLAPAR
CCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]