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The map label for this gene is proS2 [H]

Identifier: 220915162

GI number: 220915162

Start: 40794

End: 42320

Strand: Direct

Name: proS2 [H]

Synonym: A2cp1_0039

Alternate gene names: 220915162

Gene position: 40794-42320 (Clockwise)

Preceding gene: 220915161

Following gene: 220915165

Centisome position: 0.81

GC content: 70.53

Gene sequence:

>1527_bases
ATGGCCAGCGCCGAGACCGCGATCCGCCCGACCCGGGCCGAGAACTTCTCCGAGTGGTACCTCGAGGTCATCAAGGCCGC
CGATCTCGCCGAGAGCTCGACCGTCCGCGGCTGCATGGTGATCAAGCCGTGGGGCTATGCGCTCTGGGAGCACGTGCAGC
GGGTGCTGGACGGCATGTTCAAGGCCACCGGCCACGTGAACGCCTACTTCCCGCTGTTCATCCCGCTGTCGCTGCTCGAG
AAGGAGGCCGCGCACGTCGAGGGGTTCGCGAAGGAGTGCGCGGTGGTCACCCACCACCGGCTCGAGGCCCGCGACGGGAA
GCTGGTGCCGGTGGGCGAGCTCGAGGAGCCGCTCGTCGTGCGGCCGACCTCGGAGACCATCATCGGGGAGAGCTTCGCGC
GCTGGGTGCAGAGCTACCGGGACCTGCCGCTGCTCATCAACCAGTGGGCGAACGTCGTCCGCTGGGAGATGCGGACCCGG
ATGTTCCTGCGCACGGCCGAGTTCCTGTGGCAGGAGGGCCACACGGCGCACGCCACCGAGCAGGAGGCGATCGACGAGAC
CATGCAGATGCTCGGGATCTACGCCCGCTTCGCCGAGGAGTGGATGGCGCTGCCGGTGGTCCAGGGCGAGAAGACCGCGA
GCGAGCGGTTCCCGGGCGCGGTCCGCACCTACTGCATCGAGGCGATGATGCAGGACCGCAAGGCGCTGCAGGCCGGCACC
TCGCACTTCCTCGGCCAGAACTTCGCGAGGGCCTCGGGGATCCAGTTCCAGGACGAGAAGGGCGCGCTCACGCACGCCTG
GACCACGTCCTGGGGGCTCTCCACCCGGATGATCGGCGCGATGATCATGACCCACGGCGACGACGACGGCATGGTGTGCC
CGCCGCGGCTCGCGCCGCAGCAGGTGGTGATCATCCCGGTCATCCAGAAGCCGGAGGTCCGCGAGCAGGTGCTGGCGTGG
TGCGCCGAGCTGAAGCGGGAGCTCGAGGCCCAGAGCTACGCGGGCGCCCCGGTGCGCGTCCACCTCGACGCGCGCGACGT
CCAGGGCGCGAAGAAGTCCTGGGAGTGGATCAAGAAGGGCGCGCCGATCCGGCTCGAGGTCGGCCCGAAGGACATCGAGA
AGGGCGCGGTGTTCATGGGCCGCCGCGACCGGAAGCCGAGGGAGAAGCAGAGCGTGCCGCGGGGCGAGCTGGTCGCCGGG
GTGGGCGCGCTGCTGCAGGAGATCCAGGACGCGCTGCTAAGGCGCGCGCGGGCGGTGCGCGCCGAGCACACGCGGCCCAT
CGACACGAAGGACGAGTTCTACGCGTACTTCACGCCGCCCGCGACCGTGCGGCCCAACGATCCCACGCCCATCCACGGCG
GGTTCGCGCTCGCGCACTTCAGCGGGGATCCGGCGGTCGAGGCCCGCATCAAGGAGGACCTCGGCGTCACGGTGCGCTGC
ATCCCGCTCGAGCCGGGCGAGCCCGGCACCTGCCCGTTCACGGGGCAGCCGAGCCCGAAGCGCGTGGTCTGGGCGAAGTC
GTACTGA

Upstream 100 bases:

>100_bases
CCGCGCCGGCGCCCCGCACGGTGAGCGCCCCGGCGCGACGCCGGGCGGGCAGACGGCGCGCCTTGGCAGGGAACGCGGCG
GTGCGCTAGATCCTCCCGCC

Downstream 100 bases:

>100_bases
TGGGGCGAGCGGTCCGGGCGGCGTGACCCGAGGTCACGGCCGCGCCGGCGCCGTGGGCGCGACCCCCTCCACCCGCGTCT
TGCCGTCCGGGTCCACCTCG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase 2; ProRS 2 [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MASAETAIRPTRAENFSEWYLEVIKAADLAESSTVRGCMVIKPWGYALWEHVQRVLDGMFKATGHVNAYFPLFIPLSLLE
KEAAHVEGFAKECAVVTHHRLEARDGKLVPVGELEEPLVVRPTSETIIGESFARWVQSYRDLPLLINQWANVVRWEMRTR
MFLRTAEFLWQEGHTAHATEQEAIDETMQMLGIYARFAEEWMALPVVQGEKTASERFPGAVRTYCIEAMMQDRKALQAGT
SHFLGQNFARASGIQFQDEKGALTHAWTTSWGLSTRMIGAMIMTHGDDDGMVCPPRLAPQQVVIIPVIQKPEVREQVLAW
CAELKRELEAQSYAGAPVRVHLDARDVQGAKKSWEWIKKGAPIRLEVGPKDIEKGAVFMGRRDRKPREKQSVPRGELVAG
VGALLQEIQDALLRRARAVRAEHTRPIDTKDEFYAYFTPPATVRPNDPTPIHGGFALAHFSGDPAVEARIKEDLGVTVRC
IPLEPGEPGTCPFTGQPSPKRVVWAKSY

Sequences:

>Translated_508_residues
MASAETAIRPTRAENFSEWYLEVIKAADLAESSTVRGCMVIKPWGYALWEHVQRVLDGMFKATGHVNAYFPLFIPLSLLE
KEAAHVEGFAKECAVVTHHRLEARDGKLVPVGELEEPLVVRPTSETIIGESFARWVQSYRDLPLLINQWANVVRWEMRTR
MFLRTAEFLWQEGHTAHATEQEAIDETMQMLGIYARFAEEWMALPVVQGEKTASERFPGAVRTYCIEAMMQDRKALQAGT
SHFLGQNFARASGIQFQDEKGALTHAWTTSWGLSTRMIGAMIMTHGDDDGMVCPPRLAPQQVVIIPVIQKPEVREQVLAW
CAELKRELEAQSYAGAPVRVHLDARDVQGAKKSWEWIKKGAPIRLEVGPKDIEKGAVFMGRRDRKPREKQSVPRGELVAG
VGALLQEIQDALLRRARAVRAEHTRPIDTKDEFYAYFTPPATVRPNDPTPIHGGFALAHFSGDPAVEARIKEDLGVTVRC
IPLEPGEPGTCPFTGQPSPKRVVWAKSY
>Mature_507_residues
ASAETAIRPTRAENFSEWYLEVIKAADLAESSTVRGCMVIKPWGYALWEHVQRVLDGMFKATGHVNAYFPLFIPLSLLEK
EAAHVEGFAKECAVVTHHRLEARDGKLVPVGELEEPLVVRPTSETIIGESFARWVQSYRDLPLLINQWANVVRWEMRTRM
FLRTAEFLWQEGHTAHATEQEAIDETMQMLGIYARFAEEWMALPVVQGEKTASERFPGAVRTYCIEAMMQDRKALQAGTS
HFLGQNFARASGIQFQDEKGALTHAWTTSWGLSTRMIGAMIMTHGDDDGMVCPPRLAPQQVVIIPVIQKPEVREQVLAWC
AELKRELEAQSYAGAPVRVHLDARDVQGAKKSWEWIKKGAPIRLEVGPKDIEKGAVFMGRRDRKPREKQSVPRGELVAGV
GALLQEIQDALLRRARAVRAEHTRPIDTKDEFYAYFTPPATVRPNDPTPIHGGFALAHFSGDPAVEARIKEDLGVTVRCI
PLEPGEPGTCPFTGQPSPKRVVWAKSY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=523, Percent_Identity=39.1969407265774, Blast_Score=340, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI71991342, Length=500, Percent_Identity=38.8, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI71991346, Length=369, Percent_Identity=43.6314363143631, Blast_Score=307, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6321808, Length=425, Percent_Identity=41.4117647058824, Blast_Score=315, Evalue=9e-87,
Organism=Drosophila melanogaster, GI24649468, Length=508, Percent_Identity=36.6141732283465, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI24649466, Length=507, Percent_Identity=36.4891518737673, Blast_Score=318, Evalue=4e-87,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 56982; Mature: 56851

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAETAIRPTRAENFSEWYLEVIKAADLAESSTVRGCMVIKPWGYALWEHVQRVLDGMF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
KATGHVNAYFPLFIPLSLLEKEAAHVEGFAKECAVVTHHRLEARDGKLVPVGELEEPLVV
HHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEE
RPTSETIIGESFARWVQSYRDLPLLINQWANVVRWEMRTRMFLRTAEFLWQEGHTAHATE
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QEAIDETMQMLGIYARFAEEWMALPVVQGEKTASERFPGAVRTYCIEAMMQDRKALQAGT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SHFLGQNFARASGIQFQDEKGALTHAWTTSWGLSTRMIGAMIMTHGDDDGMVCPPRLAPQ
HHHHHCCHHHHCCCEEECCCCCEEEEEECCCCCHHHHHHHHHEEECCCCCCCCCCCCCCC
QVVIIPVIQKPEVREQVLAWCAELKRELEAQSYAGAPVRVHLDARDVQGAKKSWEWIKKG
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHCCCHHHHHHHHHCC
APIRLEVGPKDIEKGAVFMGRRDRKPREKQSVPRGELVAGVGALLQEIQDALLRRARAVR
CCEEEEECHHHHHCCCEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AEHTRPIDTKDEFYAYFTPPATVRPNDPTPIHGGFALAHFSGDPAVEARIKEDLGVTVRC
HHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEE
IPLEPGEPGTCPFTGQPSPKRVVWAKSY
EECCCCCCCCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure 
ASAETAIRPTRAENFSEWYLEVIKAADLAESSTVRGCMVIKPWGYALWEHVQRVLDGMF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
KATGHVNAYFPLFIPLSLLEKEAAHVEGFAKECAVVTHHRLEARDGKLVPVGELEEPLVV
HHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEE
RPTSETIIGESFARWVQSYRDLPLLINQWANVVRWEMRTRMFLRTAEFLWQEGHTAHATE
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QEAIDETMQMLGIYARFAEEWMALPVVQGEKTASERFPGAVRTYCIEAMMQDRKALQAGT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SHFLGQNFARASGIQFQDEKGALTHAWTTSWGLSTRMIGAMIMTHGDDDGMVCPPRLAPQ
HHHHHCCHHHHCCCEEECCCCCEEEEEECCCCCHHHHHHHHHEEECCCCCCCCCCCCCCC
QVVIIPVIQKPEVREQVLAWCAELKRELEAQSYAGAPVRVHLDARDVQGAKKSWEWIKKG
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHCCCHHHHHHHHHCC
APIRLEVGPKDIEKGAVFMGRRDRKPREKQSVPRGELVAGVGALLQEIQDALLRRARAVR
CCEEEEECHHHHHCCCEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AEHTRPIDTKDEFYAYFTPPATVRPNDPTPIHGGFALAHFSGDPAVEARIKEDLGVTVRC
HHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEE
IPLEPGEPGTCPFTGQPSPKRVVWAKSY
EECCCCCCCCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA