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The map label for this gene is lip2 [H]

Identifier: 220914853

GI number: 220914853

Start: 221150

End: 222100

Strand: Reverse

Name: lip2 [H]

Synonym: Mnod_7998

Alternate gene names: 220914853

Gene position: 222100-221150 (Counterclockwise)

Preceding gene: 220914855

Following gene: 220914852

Centisome position: 48.49

GC content: 64.56

Gene sequence:

>951_bases
ATGCTCGCAATTCATTCAGACGTGCTGGATCTGCTCCGATTGATCCGGGAGTCCGGAAACCCGCCCCTTGAGGCGCTCCC
GCCTGAGCAGAGCCGGCGCTTGTATTTGGCAGGCCGCCGATCGCTCCAGCCGCCTCCTGACCAGGTGTCACTGGTGCGCG
ATCTGCATGCACCGTCACAGGATGGTCCCGTACCCCTGCGCCTCTACCGCGGAATGGGAACCGCGGATGACGCTGTGCTG
CCCTGCCTCCTCTACTTGCACGGCGGAGGCTGGGTCGTAGGCAGCGTCGAATCACATGATGGCGTGTGCCGTCAGTTGGC
CAATCTCACTGCCGGCTGCGTCATTTCGGTCGAGTACCGCTTGGCCCCCGAGCACCCGTTCCCAGCTGCTGTCGAGGATG
CCGCTGCGGCTCTGACCTACGTGGTCGCACAGGCCGAGAGCTTGATGATCGACCCTGCCCGGATCGCAGTCGGGGGTGAC
AGCGCCGGCGGCAATCTCGCGGCGGTTCTCGCTCTCATGGGGCGGGATGGTGTGGTGCCGCGGACCGTCTTCCAGGCCCT
GCTCTATCCCGCCGTGGACCTGACTATGACGAGCGACGGCTATCGGCGCGTCACAGAGGGCATGCCGCTCACGGCCGCGA
CGATGCGCTACTTCGTCAGCCACTACGTACCCCATCATCCGGAGCGGTTGGACTGGCGCGCCTCTCCTTTGCGGACCACG
AGCCTGGCAGGAGCACCGCCTGCCCTGGTCCTGACCTGCGGTCATGACCCACTCTGTGAGGAGGGCAGGCTCTATGCCAA
ACGGCTTGAGGATGAGGGCGTACCGGTCACGGCCTTGCACCTGAGTGACCAGACCCACGGCATCTTGACCATGGGCAAGA
TCATTCGCGCTACCGCGAGCACTCTCACCTTCATGGCGCACGCATTATGCGATGCGTGGACTGCACTGTGA

Upstream 100 bases:

>100_bases
TCGACCGGCGCCCCGCGCGGACCGGCTTGGAATGCTTCCGAGCACTTCCCGGAGGCAGCATAACGCGCTCGATAGGCGTT
GAGATGCAAACGGTAATGGC

Downstream 100 bases:

>100_bases
GGTCAGCCTTGCGCAAACCGGCACCCTGTAGAGTCGGCGGCGGAGGCGAACCCGTCTACGTTTACGCTTTCGCTTCGCCG
CCTGAGCAACTCTTGATCCG

Product: Alpha/beta hydrolase fold-3 domain protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MLAIHSDVLDLLRLIRESGNPPLEALPPEQSRRLYLAGRRSLQPPPDQVSLVRDLHAPSQDGPVPLRLYRGMGTADDAVL
PCLLYLHGGGWVVGSVESHDGVCRQLANLTAGCVISVEYRLAPEHPFPAAVEDAAAALTYVVAQAESLMIDPARIAVGGD
SAGGNLAAVLALMGRDGVVPRTVFQALLYPAVDLTMTSDGYRRVTEGMPLTAATMRYFVSHYVPHHPERLDWRASPLRTT
SLAGAPPALVLTCGHDPLCEEGRLYAKRLEDEGVPVTALHLSDQTHGILTMGKIIRATASTLTFMAHALCDAWTAL

Sequences:

>Translated_316_residues
MLAIHSDVLDLLRLIRESGNPPLEALPPEQSRRLYLAGRRSLQPPPDQVSLVRDLHAPSQDGPVPLRLYRGMGTADDAVL
PCLLYLHGGGWVVGSVESHDGVCRQLANLTAGCVISVEYRLAPEHPFPAAVEDAAAALTYVVAQAESLMIDPARIAVGGD
SAGGNLAAVLALMGRDGVVPRTVFQALLYPAVDLTMTSDGYRRVTEGMPLTAATMRYFVSHYVPHHPERLDWRASPLRTT
SLAGAPPALVLTCGHDPLCEEGRLYAKRLEDEGVPVTALHLSDQTHGILTMGKIIRATASTLTFMAHALCDAWTAL
>Mature_316_residues
MLAIHSDVLDLLRLIRESGNPPLEALPPEQSRRLYLAGRRSLQPPPDQVSLVRDLHAPSQDGPVPLRLYRGMGTADDAVL
PCLLYLHGGGWVVGSVESHDGVCRQLANLTAGCVISVEYRLAPEHPFPAAVEDAAAALTYVVAQAESLMIDPARIAVGGD
SAGGNLAAVLALMGRDGVVPRTVFQALLYPAVDLTMTSDGYRRVTEGMPLTAATMRYFVSHYVPHHPERLDWRASPLRTT
SLAGAPPALVLTCGHDPLCEEGRLYAKRLEDEGVPVTALHLSDQTHGILTMGKIIRATASTLTFMAHALCDAWTAL

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157041239, Length=288, Percent_Identity=29.8611111111111, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI68051721, Length=297, Percent_Identity=28.6195286195286, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI206597554, Length=313, Percent_Identity=30.6709265175719, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI68299767, Length=278, Percent_Identity=30.2158273381295, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI226423947, Length=305, Percent_Identity=27.5409836065574, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI157041237, Length=254, Percent_Identity=29.1338582677165, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI61966717, Length=216, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI21328446, Length=90, Percent_Identity=37.7777777777778, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1786682, Length=280, Percent_Identity=27.5, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI72001146, Length=301, Percent_Identity=27.906976744186, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17567059, Length=265, Percent_Identity=31.3207547169811, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71996133, Length=132, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17540028, Length=144, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI115533410, Length=90, Percent_Identity=38.8888888888889, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115533412, Length=90, Percent_Identity=38.8888888888889, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24656084, Length=121, Percent_Identity=35.5371900826446, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24656076, Length=121, Percent_Identity=35.5371900826446, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI20130169, Length=121, Percent_Identity=35.5371900826446, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 33942; Mature: 33942

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAIHSDVLDLLRLIRESGNPPLEALPPEQSRRLYLAGRRSLQPPPDQVSLVRDLHAPSQ
CCEEHHHHHHHHHHHHHCCCCCCCCCCCHHHCEEEEECCCCCCCCCHHHHHHHHHCCCCC
DGPVPLRLYRGMGTADDAVLPCLLYLHGGGWVVGSVESHDGVCRQLANLTAGCVISVEYR
CCCEEEEEECCCCCCCHHHHHHHHEEECCCEEEECCCCCHHHHHHHHHCCCCEEEEEEEE
LAPEHPFPAAVEDAAAALTYVVAQAESLMIDPARIAVGGDSAGGNLAAVLALMGRDGVVP
ECCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCCCHHHHHHHHHCCCCCCH
RTVFQALLYPAVDLTMTSDGYRRVTEGMPLTAATMRYFVSHYVPHHPERLDWRASPLRTT
HHHHHHHHHHHHEEEECCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SLAGAPPALVLTCGHDPLCEEGRLYAKRLEDEGVPVTALHLSDQTHGILTMGKIIRATAS
CCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
TLTFMAHALCDAWTAL
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLAIHSDVLDLLRLIRESGNPPLEALPPEQSRRLYLAGRRSLQPPPDQVSLVRDLHAPSQ
CCEEHHHHHHHHHHHHHCCCCCCCCCCCHHHCEEEEECCCCCCCCCHHHHHHHHHCCCCC
DGPVPLRLYRGMGTADDAVLPCLLYLHGGGWVVGSVESHDGVCRQLANLTAGCVISVEYR
CCCEEEEEECCCCCCCHHHHHHHHEEECCCEEEECCCCCHHHHHHHHHCCCCEEEEEEEE
LAPEHPFPAAVEDAAAALTYVVAQAESLMIDPARIAVGGDSAGGNLAAVLALMGRDGVVP
ECCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCCCHHHHHHHHHCCCCCCH
RTVFQALLYPAVDLTMTSDGYRRVTEGMPLTAATMRYFVSHYVPHHPERLDWRASPLRTT
HHHHHHHHHHHHEEEECCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SLAGAPPALVLTCGHDPLCEEGRLYAKRLEDEGVPVTALHLSDQTHGILTMGKIIRATAS
CCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
TLTFMAHALCDAWTAL
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]