| Definition | Methylobacterium nodulans ORS 2060 plasmid pMNOD02, complete sequence. |
|---|---|
| Accession | NC_011887 |
| Length | 458,070 |
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The map label for this gene is iolC [H]
Identifier: 220914695
GI number: 220914695
Start: 32851
End: 34773
Strand: Reverse
Name: iolC [H]
Synonym: Mnod_7803
Alternate gene names: 220914695
Gene position: 34773-32851 (Counterclockwise)
Preceding gene: 220914696
Following gene: 220914694
Centisome position: 7.59
GC content: 67.76
Gene sequence:
>1923_bases ATGAAGACGTTGGATGTCATCACCATCGGCCGTTCGTCGGTCGACCTCTACGGGACCCAGATTGGCGGCCGGCTCGAGGA CATGCGGTCGTTCCAGAAGTATATCGGCGGGTCTCCGACCAATATCGCGGCGGGGACGGCGCGGCTGGGGCTGAAGTCGG CGTTGATCACCCGGGTCGGCGACGAGCATATGGGGCGGTTCATTCGCGAGGAGCTGCTCAAGGAGGGCGTGGACGTGCGC GGGGTCAAGACCGACCCCGAGCGCCTGACCGCGCTGGTGCTGCTGGGTATTCGCGACCAGGAGCGCTTTCCGCTGATCTT CTACCGCAGCGACTGCGCGGACATGGCGCTGTGCGAGGACGATATCGACGAGAGCCTGATTGCGCAGGCGCGCTCGGTCG TGGCCACCGGCACGCATCTGAGCCACCCGCGGACCGAGGCGGCCGTGCTGAAGGCCTTTCGGCTCGCCAGGACGCATGGC GCGCGCACGGCGCTCGACATCGACTATCGCCCGAACCTCTGGGGCCTCGCTGGCCATGATGCCGGCGAGAGTCGTTTCAT CGCGAGCAAGGCGGTGACGGCAAAACTGCAAAGCCACCTGCCTTTGTTCGACCTGATCGTCGGCACCGAGGAGGAGTTCC ACATCGCCGGTGGGGATACGAACACGCTTGCCGCGCTGCGGGCCGTGCGCGCCGTCTCCTCCGCGGTGCTAGTCTGCAAG CGCGGGGCGGCGGGGGCCGTGGCCTTCACCGGCGAGATCCCCGACAGCCTCGATGAGGGCGAGATGGGTCCGGGCTTTCC GATCGAGGTGTTCAATGTCCTCGGCGCGGGCGACGGTTTCATGTCGGGCCTGATCAAGGGATGGCTCGAGGAGGCGCCGT GGCCGCGGGCCTTGGCATATGCCAATGCCTGCGGGGCGTTTGCGGTCAGCCGTCATGGCTGCACGCCGGCCTATCCCTCG CTGACGGAGCTGGAATTCTTCCTCGAACGCGGCGTGGTCACGCCGGCTTTGCGCCATGACGCGGCGCTGGAGCAGCTTCA CTGGTCCACCAACCGCCACGGCGACTGGTCGACCATGCGGGTCTTCGCCTTCGATCACCGGATGCAGCTGGAGGTGATGG AGGGCGCCAGCCCGGCGAAGATCGGCGCCTTCAAGGAACTGTGCCTGCAGGCTGCTCTCGAAGTCCAGGATGGACGGCCA GGCTATGGCATCCTGTGCGACGACCGGCTGGGACGGGACGCGCTGCAGGCCGCCGCGGGAACCGGCCTCTGGATCGGCCG GCCGGTGGAGTGGCCGGGCTCGCGGCCGCTGACGCTCGAACCCGAGCTCGGTCCCGACTACGGCGGGCTGGCCGAATGGC CGCTCGACCATGTGGTGAAGGTGCTGTGTTTCTGCCATCCGGACGATACCCTGGAGATGCGGGCGCAGCAGGAGGCGGTG GTGACGCGGCTCTTCCATGCCGCGCGCCGCCAGCGGCTCGAATTTCTGTTGGAGATTATCCCCTCGAAGGTCGGGCCGGT CGATGACCGCACGACCGCAACCCTGATCCGCCGCTTCTACGAGATCGGGATCTACCCGGACTGGTGGAAGCTGGAGCCGA TGGCGAGCGCGGTGGCCTGGGCCGAGGCTTGCGCGGCCATTGAGGAACACGACCGCCATACGCGCGGCATCGTCGTGCTG GGGCTGGATGCGCCCGAGGCGGAGCTGGCCGAGAGCTTCCGGATCGCGGCCGGGTTTAGTCTCGTGAAAGGCTTTGCCGT GGGGCGGACCATCTTCGGCCAGGTGGCGCGCGACTGGCTGGCAGGCCGGATCGGCGATGAGGACGCGGTTGCGGAGATGG TGCGCCGCTATGCGCGGCTTTGCGCAGTCTGGGATGCCGGGAAGGGCCTTGCGAGTGCAACGGCCGGACGGGAGCAGGCA TGA
Upstream 100 bases:
>100_bases GGCGGAGGACAGCGCCCGCTTCATGCTCGCGGAAATGAAGCGCTACGTGGGGACATACTGGAAAGCCTGATCAGGGTGGG CAGGCGACGGAGAGGCAGAG
Downstream 100 bases:
>100_bases TGACGACGGTGCGGTTGACGGCGGCGCAGACCATGATGCGCTGGCTTTCGGTGCAGATGACCGAAGAAGGCGAGCGGTTC ATCGAGGGGGTCTGGGCGAT
Product: PfkB domain protein
Products: NA
Alternate protein names: 2-deoxy-5-keto-D-gluconate kinase; DKG kinase [H]
Number of amino acids: Translated: 640; Mature: 640
Protein sequence:
>640_residues MKTLDVITIGRSSVDLYGTQIGGRLEDMRSFQKYIGGSPTNIAAGTARLGLKSALITRVGDEHMGRFIREELLKEGVDVR GVKTDPERLTALVLLGIRDQERFPLIFYRSDCADMALCEDDIDESLIAQARSVVATGTHLSHPRTEAAVLKAFRLARTHG ARTALDIDYRPNLWGLAGHDAGESRFIASKAVTAKLQSHLPLFDLIVGTEEEFHIAGGDTNTLAALRAVRAVSSAVLVCK RGAAGAVAFTGEIPDSLDEGEMGPGFPIEVFNVLGAGDGFMSGLIKGWLEEAPWPRALAYANACGAFAVSRHGCTPAYPS LTELEFFLERGVVTPALRHDAALEQLHWSTNRHGDWSTMRVFAFDHRMQLEVMEGASPAKIGAFKELCLQAALEVQDGRP GYGILCDDRLGRDALQAAAGTGLWIGRPVEWPGSRPLTLEPELGPDYGGLAEWPLDHVVKVLCFCHPDDTLEMRAQQEAV VTRLFHAARRQRLEFLLEIIPSKVGPVDDRTTATLIRRFYEIGIYPDWWKLEPMASAVAWAEACAAIEEHDRHTRGIVVL GLDAPEAELAESFRIAAGFSLVKGFAVGRTIFGQVARDWLAGRIGDEDAVAEMVRRYARLCAVWDAGKGLASATAGREQA
Sequences:
>Translated_640_residues MKTLDVITIGRSSVDLYGTQIGGRLEDMRSFQKYIGGSPTNIAAGTARLGLKSALITRVGDEHMGRFIREELLKEGVDVR GVKTDPERLTALVLLGIRDQERFPLIFYRSDCADMALCEDDIDESLIAQARSVVATGTHLSHPRTEAAVLKAFRLARTHG ARTALDIDYRPNLWGLAGHDAGESRFIASKAVTAKLQSHLPLFDLIVGTEEEFHIAGGDTNTLAALRAVRAVSSAVLVCK RGAAGAVAFTGEIPDSLDEGEMGPGFPIEVFNVLGAGDGFMSGLIKGWLEEAPWPRALAYANACGAFAVSRHGCTPAYPS LTELEFFLERGVVTPALRHDAALEQLHWSTNRHGDWSTMRVFAFDHRMQLEVMEGASPAKIGAFKELCLQAALEVQDGRP GYGILCDDRLGRDALQAAAGTGLWIGRPVEWPGSRPLTLEPELGPDYGGLAEWPLDHVVKVLCFCHPDDTLEMRAQQEAV VTRLFHAARRQRLEFLLEIIPSKVGPVDDRTTATLIRRFYEIGIYPDWWKLEPMASAVAWAEACAAIEEHDRHTRGIVVL GLDAPEAELAESFRIAAGFSLVKGFAVGRTIFGQVARDWLAGRIGDEDAVAEMVRRYARLCAVWDAGKGLASATAGREQA >Mature_640_residues MKTLDVITIGRSSVDLYGTQIGGRLEDMRSFQKYIGGSPTNIAAGTARLGLKSALITRVGDEHMGRFIREELLKEGVDVR GVKTDPERLTALVLLGIRDQERFPLIFYRSDCADMALCEDDIDESLIAQARSVVATGTHLSHPRTEAAVLKAFRLARTHG ARTALDIDYRPNLWGLAGHDAGESRFIASKAVTAKLQSHLPLFDLIVGTEEEFHIAGGDTNTLAALRAVRAVSSAVLVCK RGAAGAVAFTGEIPDSLDEGEMGPGFPIEVFNVLGAGDGFMSGLIKGWLEEAPWPRALAYANACGAFAVSRHGCTPAYPS LTELEFFLERGVVTPALRHDAALEQLHWSTNRHGDWSTMRVFAFDHRMQLEVMEGASPAKIGAFKELCLQAALEVQDGRP GYGILCDDRLGRDALQAAAGTGLWIGRPVEWPGSRPLTLEPELGPDYGGLAEWPLDHVVKVLCFCHPDDTLEMRAQQEAV VTRLFHAARRQRLEFLLEIIPSKVGPVDDRTTATLIRRFYEIGIYPDWWKLEPMASAVAWAEACAAIEEHDRHTRGIVVL GLDAPEAELAESFRIAAGFSLVKGFAVGRTIFGQVARDWLAGRIGDEDAVAEMVRRYARLCAVWDAGKGLASATAGREQA
Specific function: Catalyzes the phosphorylation of 5-dehydro-2-deoxy-D- gluconate (2-deoxy-5-keto-D-gluconate or DKG) to 6-phospho-5- dehydro-2-deoxy-D-gluconate (DKGP) [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the carbohydrate kinase pfkB family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR011611 - InterPro: IPR002173 - InterPro: IPR022841 [H]
Pfam domain/function: PF00294 PfkB [H]
EC number: =2.7.1.92 [H]
Molecular weight: Translated: 69777; Mature: 69777
Theoretical pI: Translated: 5.52; Mature: 5.52
Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.7 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 3.6 %Cys+Met (Translated Protein) 1.7 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKTLDVITIGRSSVDLYGTQIGGRLEDMRSFQKYIGGSPTNIAAGTARLGLKSALITRVG CCCEEEEEECCCCCEEEHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC DEHMGRFIREELLKEGVDVRGVKTDPERLTALVLLGIRDQERFPLIFYRSDCADMALCED HHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEHHEEECCCCCCCCEEEEECCCCHHHHHHH DIDESLIAQARSVVATGTHLSHPRTEAAVLKAFRLARTHGARTALDIDYRPNLWGLAGHD CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEECCCC AGESRFIASKAVTAKLQSHLPLFDLIVGTEEEFHIAGGDTNTLAALRAVRAVSSAVLVCK CCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCC RGAAGAVAFTGEIPDSLDEGEMGPGFPIEVFNVLGAGDGFMSGLIKGWLEEAPWPRALAY CCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHH ANACGAFAVSRHGCTPAYPSLTELEFFLERGVVTPALRHDAALEQLHWSTNRHGDWSTMR HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEE VFAFDHRMQLEVMEGASPAKIGAFKELCLQAALEVQDGRPGYGILCDDRLGRDALQAAAG EEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCC TGLWIGRPVEWPGSRPLTLEPELGPDYGGLAEWPLDHVVKVLCFCHPDDTLEMRAQQEAV CCCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH VTRLFHAARRQRLEFLLEIIPSKVGPVDDRTTATLIRRFYEIGIYPDWWKLEPMASAVAW HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH AEACAAIEEHDRHTRGIVVLGLDAPEAELAESFRIAAGFSLVKGFAVGRTIFGQVARDWL HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH AGRIGDEDAVAEMVRRYARLCAVWDAGKGLASATAGREQA HCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCC >Mature Secondary Structure MKTLDVITIGRSSVDLYGTQIGGRLEDMRSFQKYIGGSPTNIAAGTARLGLKSALITRVG CCCEEEEEECCCCCEEEHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC DEHMGRFIREELLKEGVDVRGVKTDPERLTALVLLGIRDQERFPLIFYRSDCADMALCED HHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEHHEEECCCCCCCCEEEEECCCCHHHHHHH DIDESLIAQARSVVATGTHLSHPRTEAAVLKAFRLARTHGARTALDIDYRPNLWGLAGHD CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEECCCC AGESRFIASKAVTAKLQSHLPLFDLIVGTEEEFHIAGGDTNTLAALRAVRAVSSAVLVCK CCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCC RGAAGAVAFTGEIPDSLDEGEMGPGFPIEVFNVLGAGDGFMSGLIKGWLEEAPWPRALAY CCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHH ANACGAFAVSRHGCTPAYPSLTELEFFLERGVVTPALRHDAALEQLHWSTNRHGDWSTMR HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEE VFAFDHRMQLEVMEGASPAKIGAFKELCLQAALEVQDGRPGYGILCDDRLGRDALQAAAG EEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCC TGLWIGRPVEWPGSRPLTLEPELGPDYGGLAEWPLDHVVKVLCFCHPDDTLEMRAQQEAV CCCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH VTRLFHAARRQRLEFLLEIIPSKVGPVDDRTTATLIRRFYEIGIYPDWWKLEPMASAVAW HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH AEACAAIEEHDRHTRGIVVLGLDAPEAELAESFRIAAGFSLVKGFAVGRTIFGQVARDWL HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH AGRIGDEDAVAEMVRRYARLCAVWDAGKGLASATAGREQA HCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA