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The map label for this gene is eno [H]

Identifier: 220914679

GI number: 220914679

Start: 11380

End: 12660

Strand: Reverse

Name: eno [H]

Synonym: Mnod_7783

Alternate gene names: 220914679

Gene position: 12660-11380 (Counterclockwise)

Preceding gene: 220914681

Following gene: 220914678

Centisome position: 2.76

GC content: 62.76

Gene sequence:

>1281_bases
ATGACGTGCACGCGGCTCCGACGGCTTCATGCTCGCCAGATCCTCGATTCTCGCGGGCGCCCAACCGTCGAGGCAGAAAT
CAGCTTGGACAATGGTATCCAGGTCCGCGCCTCTGTCCCGTCTGGCGCTTCTACGGGAAAGGCTGAAGCCGTCGAACTGC
GCGATGGCGGTGAGGCCTGGGGCGGTCTCGGGGTCAGTCGCGCCGTTTCCAACATCCGTGGCGAGATCGCGGCTCTGCTC
GAGGGCTGCGACGTTCTTGACCAAGTCGGCCTCGACAACCGAATCCGCGAGGCGGATGGCACTCACAACCTTGCTCGTTT
TGGAGCAAACGCGACGCTGGCTGTGAGCATCGCGGCTGTACGCGCTTCCGCTGTCGCTACAGGAATACCCCTTCATCGCC
GGCTGGCCGAGTTGTGCGGCACGACCCCGTCCATGCCGATGCCGATGGTCAACATCCTCTCGGGCGGGCTGCATGCTGGG
CGCGGCATGGACGTCCAGGATTTCCTGGTGATCCCCATCGGTGCTTCCTCCTATTCTGAGGCCCTTGATTGGATCTGTCG
GGTCCGCACAGCCGCAGCCAGCATCTGTGCCAAGCGCGGCATTTCCACCTTACTCGCCGACGAAGGAGGCTTGTCCCCCG
GATTTGCCACGTCAGAGGAGGCATTGTCTCTTATGGTCGAGGCGTTCGAGCAAGCAGGCCTTGAGCCCGGCGTTCAGGCG
GCTATTGCCCTGGACGTTGCCTCGTCCTCGCTCGCCAGCGCGAACGGTGCCTACGCGTTTGCTCGGCAGGGCCGCAGTTT
CGGTGCACGCGAGATGATCGAACTACAACGACAATGGGCCGCCTCCTTTCCGATCGTGTCGATCGAGGATGGTCTCGGCG
AAGATGACTGGGCAAACTGGCCAGCTCTGACGCGAGCGCTCGGCCATCTTCAGCTTGTGGGCGACGACCTATTCGCAACA
CAGCCTGATCGCATCCGTCGAGGGATCGACGAGAATATCGCGAACGCTGCCCTCATCAAAGTCAATCAGAACGGAACGTT
GTCCGGCACGCTGGAGGCGATCACGACTGCCCGGGCGGCTGGTTATGCCACCGTCATCTCGGCCCGCTCGGGCGAGACGG
AGGATGATTTCCTGGCCGATTTGGCAGTCGGTGTGGCGGGTAGGCAGATCAAGGTCGGCTCGGTGCGCAACTCCGAGCGG
CTGGCCAAGTACAATCAACTGCTCCGCCTTGAGGAGGAAGGGCTTGCCTGGTCCGGCCGAACAGGTCTGGCACCCCTATG
A

Upstream 100 bases:

>100_bases
GCGGCTCCGCCCCGACCGGCAGCGACACACACGCCGATATCTGCTCTACAGGATCGCATGCACGCCGCTGGCGCTGATGC
TTTCAGGGGGAGGGATGCTC

Downstream 100 bases:

>100_bases
ATGCTCCCGAGCCCCTTCTCGCTGACTTTCTGCGCCGAATGAGGCTTGCGCCTCCAGGCGAGGAGGGCTGCTGGATCCCG
CTCTCAGGCGGCGTGTCATC

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTCTRLRRLHARQILDSRGRPTVEAEISLDNGIQVRASVPSGASTGKAEAVELRDGGEAWGGLGVSRAVSNIRGEIAALL
EGCDVLDQVGLDNRIREADGTHNLARFGANATLAVSIAAVRASAVATGIPLHRRLAELCGTTPSMPMPMVNILSGGLHAG
RGMDVQDFLVIPIGASSYSEALDWICRVRTAAASICAKRGISTLLADEGGLSPGFATSEEALSLMVEAFEQAGLEPGVQA
AIALDVASSSLASANGAYAFARQGRSFGAREMIELQRQWAASFPIVSIEDGLGEDDWANWPALTRALGHLQLVGDDLFAT
QPDRIRRGIDENIANAALIKVNQNGTLSGTLEAITTARAAGYATVISARSGETEDDFLADLAVGVAGRQIKVGSVRNSER
LAKYNQLLRLEEEGLAWSGRTGLAPL

Sequences:

>Translated_426_residues
MTCTRLRRLHARQILDSRGRPTVEAEISLDNGIQVRASVPSGASTGKAEAVELRDGGEAWGGLGVSRAVSNIRGEIAALL
EGCDVLDQVGLDNRIREADGTHNLARFGANATLAVSIAAVRASAVATGIPLHRRLAELCGTTPSMPMPMVNILSGGLHAG
RGMDVQDFLVIPIGASSYSEALDWICRVRTAAASICAKRGISTLLADEGGLSPGFATSEEALSLMVEAFEQAGLEPGVQA
AIALDVASSSLASANGAYAFARQGRSFGAREMIELQRQWAASFPIVSIEDGLGEDDWANWPALTRALGHLQLVGDDLFAT
QPDRIRRGIDENIANAALIKVNQNGTLSGTLEAITTARAAGYATVISARSGETEDDFLADLAVGVAGRQIKVGSVRNSER
LAKYNQLLRLEEEGLAWSGRTGLAPL
>Mature_425_residues
TCTRLRRLHARQILDSRGRPTVEAEISLDNGIQVRASVPSGASTGKAEAVELRDGGEAWGGLGVSRAVSNIRGEIAALLE
GCDVLDQVGLDNRIREADGTHNLARFGANATLAVSIAAVRASAVATGIPLHRRLAELCGTTPSMPMPMVNILSGGLHAGR
GMDVQDFLVIPIGASSYSEALDWICRVRTAAASICAKRGISTLLADEGGLSPGFATSEEALSLMVEAFEQAGLEPGVQAA
IALDVASSSLASANGAYAFARQGRSFGAREMIELQRQWAASFPIVSIEDGLGEDDWANWPALTRALGHLQLVGDDLFATQ
PDRIRRGIDENIANAALIKVNQNGTLSGTLEAITTARAAGYATVISARSGETEDDFLADLAVGVAGRQIKVGSVRNSERL
AKYNQLLRLEEEGLAWSGRTGLAPL

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI4503571, Length=433, Percent_Identity=40.6466512702078, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI301897477, Length=418, Percent_Identity=42.1052631578947, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI301897469, Length=418, Percent_Identity=42.1052631578947, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI5803011, Length=419, Percent_Identity=42.2434367541766, Blast_Score=305, Evalue=7e-83,
Organism=Homo sapiens, GI301897479, Length=415, Percent_Identity=38.5542168674699, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI169201331, Length=347, Percent_Identity=27.0893371757925, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI169201757, Length=347, Percent_Identity=27.0893371757925, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI239744207, Length=347, Percent_Identity=27.0893371757925, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=46.0093896713615, Blast_Score=356, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17536383, Length=420, Percent_Identity=43.5714285714286, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI71995829, Length=420, Percent_Identity=43.5714285714286, Blast_Score=320, Evalue=6e-88,
Organism=Caenorhabditis elegans, GI32563855, Length=181, Percent_Identity=44.1988950276243, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324974, Length=424, Percent_Identity=42.2169811320755, Blast_Score=300, Evalue=4e-82,
Organism=Saccharomyces cerevisiae, GI6324969, Length=424, Percent_Identity=42.2169811320755, Blast_Score=300, Evalue=4e-82,
Organism=Saccharomyces cerevisiae, GI6323985, Length=423, Percent_Identity=41.8439716312057, Blast_Score=299, Evalue=7e-82,
Organism=Saccharomyces cerevisiae, GI6321693, Length=422, Percent_Identity=42.1800947867299, Blast_Score=296, Evalue=4e-81,
Organism=Saccharomyces cerevisiae, GI6321968, Length=422, Percent_Identity=41.9431279620853, Blast_Score=277, Evalue=3e-75,
Organism=Drosophila melanogaster, GI281360527, Length=435, Percent_Identity=39.7701149425287, Blast_Score=295, Evalue=6e-80,
Organism=Drosophila melanogaster, GI17137654, Length=435, Percent_Identity=39.7701149425287, Blast_Score=295, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24580918, Length=435, Percent_Identity=39.7701149425287, Blast_Score=294, Evalue=9e-80,
Organism=Drosophila melanogaster, GI24580916, Length=435, Percent_Identity=39.7701149425287, Blast_Score=294, Evalue=9e-80,
Organism=Drosophila melanogaster, GI24580920, Length=435, Percent_Identity=39.7701149425287, Blast_Score=294, Evalue=9e-80,
Organism=Drosophila melanogaster, GI24580914, Length=435, Percent_Identity=39.7701149425287, Blast_Score=294, Evalue=9e-80,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 44779; Mature: 44648

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCTRLRRLHARQILDSRGRPTVEAEISLDNGIQVRASVPSGASTGKAEAVELRDGGEAW
CCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCEEEEECCCCCHH
GGLGVSRAVSNIRGEIAALLEGCDVLDQVGLDNRIREADGTHNLARFGANATLAVSIAAV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHCCCCEEEEEHHHH
RASAVATGIPLHRRLAELCGTTPSMPMPMVNILSGGLHAGRGMDVQDFLVIPIGASSYSE
HHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHH
ALDWICRVRTAAASICAKRGISTLLADEGGLSPGFATSEEALSLMVEAFEQAGLEPGVQA
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
AIALDVASSSLASANGAYAFARQGRSFGAREMIELQRQWAASFPIVSIEDGLGEDDWANW
EEEEEHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
PALTRALGHLQLVGDDLFATQPDRIRRGIDENIANAALIKVNQNGTLSGTLEAITTARAA
HHHHHHHHHHHEECCHHHCCCHHHHHHCHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHC
GYATVISARSGETEDDFLADLAVGVAGRQIKVGSVRNSERLAKYNQLLRLEEEGLAWSGR
CCEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEECCC
TGLAPL
CCCCCC
>Mature Secondary Structure 
TCTRLRRLHARQILDSRGRPTVEAEISLDNGIQVRASVPSGASTGKAEAVELRDGGEAW
CHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCEEEEECCCCCHH
GGLGVSRAVSNIRGEIAALLEGCDVLDQVGLDNRIREADGTHNLARFGANATLAVSIAAV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHCCCCEEEEEHHHH
RASAVATGIPLHRRLAELCGTTPSMPMPMVNILSGGLHAGRGMDVQDFLVIPIGASSYSE
HHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHH
ALDWICRVRTAAASICAKRGISTLLADEGGLSPGFATSEEALSLMVEAFEQAGLEPGVQA
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
AIALDVASSSLASANGAYAFARQGRSFGAREMIELQRQWAASFPIVSIEDGLGEDDWANW
EEEEEHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
PALTRALGHLQLVGDDLFATQPDRIRRGIDENIANAALIKVNQNGTLSGTLEAITTARAA
HHHHHHHHHHHEECCHHHCCCHHHHHHCHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHC
GYATVISARSGETEDDFLADLAVGVAGRQIKVGSVRNSERLAKYNQLLRLEEEGLAWSGR
CCEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEECCC
TGLAPL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA