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The map label for this gene is paaK [H]

Identifier: 220905598

GI number: 220905598

Start: 2816061

End: 2817341

Strand: Reverse

Name: paaK [H]

Synonym: Ddes_2338

Alternate gene names: 220905598

Gene position: 2817341-2816061 (Counterclockwise)

Preceding gene: 220905599

Following gene: 220905597

Centisome position: 98.05

GC content: 62.76

Gene sequence:

>1281_bases
ATGTCATACCGCTTCATTCCACAGCTTGCGGATGAGGAAATCCGCCTCCAGCAGGAGGAAGGCCTGTGCCGGACAGTGCG
CCGCGCCTGGAACTCACCCCAGTACAGCAGCAAGTTGCGCGCCTGTGGGCTTGAGCCTGGTGAGGCCATCTGCCTGGACG
ATCTGCCGCGCCTGCCCACTGTGGATGTGGACGATCTGCGCGAGGGCTATCCCTTGCCCCTGCTGTGCGTGCCTCCCGCT
GCCGTGGTGCGTGTTCACGCCTCCAGCGGCACGACGGGCAAGCGCAAGATCCTGGCCTATACCGCGGCGGACGTGGAAAC
ATTCAATCTGCAGATGGCCCGCTGTTACGAGTTGGCCGGGCTGACCGCTGACGACCGCATGCAGATAGCCGTGGGTTATG
GCCTGTGGACGGCCGGAGTGGGTTTTCAGGGCGGCAGCGAAAAGCTCGGCATGCTTACCGTGCCTGTGGGGCCGGGCAAC
CTTGAGATGCATTTGCAGCTTTTGCAGGACCTTGAATCCACCTGTTTCGGCGCTACAGCCTCCATGGCCCTGCTACTGGC
CGAAGAGGTGGAGCGGTCGGGCCTTGGCGGGCGGCTCAAGCTGCGCAAGATGATCTGCGGGTCTGAGGCGCGCAGCGAAA
AAATGCGACAAACCATTGAAAGCAAGCTGGGGCTTGAAGGTTGCCACGATATAGCGGGCATGACCGAAATGTATGGTCCC
GGTACGGCCATAGACTGCGATGCCCACGACGGTCTGCATTACTGGGCGGACCTGTTCATCATCGAGGTACTTGACCCCGT
CACGTTGCAGCCGGTGGCGGAGGGAGAAGTGGGCGAGATGGTGGTGACCAGCCTGCGCAAGGAGGCCGTACCCCTGCTGC
GTTACCGCACCCATGACCTGTGCCGCCTGCTGCCGGGGCGCTGCGCCTGCGGCCTGAACATGCCGCGCCATGACCGCATT
CTGGGCCGTTCAGACGACATGCTCATCTACCGGGGCGTCAATATCTACCCCGGCCAGTTCATGGCTGTAATCGGGGAATT
TGCCGAACTGGGCGGCGAGTATCAGGTGGACCTCAGCCGCGACGAGCGCGGTCTCGACCATCTGGCGCTCACAGTGGAGC
GCGCCCAGAACGCCTGCGGGGGCAATGATTCCGCCCTGGCTTCTGCGCTGGAAAAACGCCTGCACAAGGCCATCATGGCG
CGTATGGATGTCAGTATTGTGGATTATGCCGCCTTGCCGCGCACGTTCAGCAAGTCCCGTCGCGTGGTGGACAGGCGCTG
A

Upstream 100 bases:

>100_bases
GTGTGCAGCCGGTTTGTGCCTGTTTTCAGAAAATACATGAGGTTATTGCCACCAGGCATTGCAGATGAACCTGCCTCAAG
GGTAAAGGAGCCGCAGAAGC

Downstream 100 bases:

>100_bases
TCTGCGATGGGCAAAGGACTGCCGGTCCGTTCAGTCGCCCATATAGTGGCCGTTTGTGGGGCCTGCCGTGACCGTGGCCC
CTGAAAGCTGTTGGGCTGCG

Product: Phenylacetate--CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSYRFIPQLADEEIRLQQEEGLCRTVRRAWNSPQYSSKLRACGLEPGEAICLDDLPRLPTVDVDDLREGYPLPLLCVPPA
AVVRVHASSGTTGKRKILAYTAADVETFNLQMARCYELAGLTADDRMQIAVGYGLWTAGVGFQGGSEKLGMLTVPVGPGN
LEMHLQLLQDLESTCFGATASMALLLAEEVERSGLGGRLKLRKMICGSEARSEKMRQTIESKLGLEGCHDIAGMTEMYGP
GTAIDCDAHDGLHYWADLFIIEVLDPVTLQPVAEGEVGEMVVTSLRKEAVPLLRYRTHDLCRLLPGRCACGLNMPRHDRI
LGRSDDMLIYRGVNIYPGQFMAVIGEFAELGGEYQVDLSRDERGLDHLALTVERAQNACGGNDSALASALEKRLHKAIMA
RMDVSIVDYAALPRTFSKSRRVVDRR

Sequences:

>Translated_426_residues
MSYRFIPQLADEEIRLQQEEGLCRTVRRAWNSPQYSSKLRACGLEPGEAICLDDLPRLPTVDVDDLREGYPLPLLCVPPA
AVVRVHASSGTTGKRKILAYTAADVETFNLQMARCYELAGLTADDRMQIAVGYGLWTAGVGFQGGSEKLGMLTVPVGPGN
LEMHLQLLQDLESTCFGATASMALLLAEEVERSGLGGRLKLRKMICGSEARSEKMRQTIESKLGLEGCHDIAGMTEMYGP
GTAIDCDAHDGLHYWADLFIIEVLDPVTLQPVAEGEVGEMVVTSLRKEAVPLLRYRTHDLCRLLPGRCACGLNMPRHDRI
LGRSDDMLIYRGVNIYPGQFMAVIGEFAELGGEYQVDLSRDERGLDHLALTVERAQNACGGNDSALASALEKRLHKAIMA
RMDVSIVDYAALPRTFSKSRRVVDRR
>Mature_425_residues
SYRFIPQLADEEIRLQQEEGLCRTVRRAWNSPQYSSKLRACGLEPGEAICLDDLPRLPTVDVDDLREGYPLPLLCVPPAA
VVRVHASSGTTGKRKILAYTAADVETFNLQMARCYELAGLTADDRMQIAVGYGLWTAGVGFQGGSEKLGMLTVPVGPGNL
EMHLQLLQDLESTCFGATASMALLLAEEVERSGLGGRLKLRKMICGSEARSEKMRQTIESKLGLEGCHDIAGMTEMYGPG
TAIDCDAHDGLHYWADLFIIEVLDPVTLQPVAEGEVGEMVVTSLRKEAVPLLRYRTHDLCRLLPGRCACGLNMPRHDRIL
GRSDDMLIYRGVNIYPGQFMAVIGEFAELGGEYQVDLSRDERGLDHLALTVERAQNACGGNDSALASALEKRLHKAIMAR
MDVSIVDYAALPRTFSKSRRVVDRR

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=424, Percent_Identity=39.1509433962264, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 46873; Mature: 46741

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYRFIPQLADEEIRLQQEEGLCRTVRRAWNSPQYSSKLRACGLEPGEAICLDDLPRLPT
CCCCCCCCCCCHHHHCCHHCCHHHHHHHHCCCCCHHHHHHHCCCCCCCEEECCCCCCCCC
VDVDDLREGYPLPLLCVPPAAVVRVHASSGTTGKRKILAYTAADVETFNLQMARCYELAG
CCHHHHHCCCCCCEEECCCCEEEEEECCCCCCCCEEEEEEEECCCEEHHHHHHHHHHHHC
LTADDRMQIAVGYGLWTAGVGFQGGSEKLGMLTVPVGPGNLEMHLQLLQDLESTCFGATA
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHH
SMALLLAEEVERSGLGGRLKLRKMICGSEARSEKMRQTIESKLGLEGCHDIAGMTEMYGP
HHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHCHHHHCCC
GTAIDCDAHDGLHYWADLFIIEVLDPVTLQPVAEGEVGEMVVTSLRKEAVPLLRYRTHDL
CCEEECCCCCCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
CRLLPGRCACGLNMPRHDRILGRSDDMLIYRGVNIYPGQFMAVIGEFAELGGEYQVDLSR
HHHCCCCEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCC
DERGLDHLALTVERAQNACGGNDSALASALEKRLHKAIMARMDVSIVDYAALPRTFSKSR
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHH
RVVDRR
HHCCCC
>Mature Secondary Structure 
SYRFIPQLADEEIRLQQEEGLCRTVRRAWNSPQYSSKLRACGLEPGEAICLDDLPRLPT
CCCCCCCCCCHHHHCCHHCCHHHHHHHHCCCCCHHHHHHHCCCCCCCEEECCCCCCCCC
VDVDDLREGYPLPLLCVPPAAVVRVHASSGTTGKRKILAYTAADVETFNLQMARCYELAG
CCHHHHHCCCCCCEEECCCCEEEEEECCCCCCCCEEEEEEEECCCEEHHHHHHHHHHHHC
LTADDRMQIAVGYGLWTAGVGFQGGSEKLGMLTVPVGPGNLEMHLQLLQDLESTCFGATA
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHH
SMALLLAEEVERSGLGGRLKLRKMICGSEARSEKMRQTIESKLGLEGCHDIAGMTEMYGP
HHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHCHHHHCCC
GTAIDCDAHDGLHYWADLFIIEVLDPVTLQPVAEGEVGEMVVTSLRKEAVPLLRYRTHDL
CCEEECCCCCCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
CRLLPGRCACGLNMPRHDRILGRSDDMLIYRGVNIYPGQFMAVIGEFAELGGEYQVDLSR
HHHCCCCEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCC
DERGLDHLALTVERAQNACGGNDSALASALEKRLHKAIMARMDVSIVDYAALPRTFSKSR
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHH
RVVDRR
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]