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The map label for this gene is cbiK [H]

Identifier: 220905594

GI number: 220905594

Start: 2809231

End: 2810145

Strand: Direct

Name: cbiK [H]

Synonym: Ddes_2334

Alternate gene names: 220905594

Gene position: 2809231-2810145 (Clockwise)

Preceding gene: 220905591

Following gene: 220905595

Centisome position: 97.77

GC content: 61.53

Gene sequence:

>915_bases
ATGCCGCGTATCCTTTCACATGCGGGGTTTCGCCCGCTGCTTTTCGTTCTGCTGCTGCTCGCGAGCCTGTGCCTGCACGG
CACAAGCCATGCCGCCGGTGCCCCCAAGGAAGGGGTGCTGCTGGTGGCCTTCGGCACCAGCGTTCCCGAGGGCATGCCCG
CCTTCAAGGCCGTAGATGCTGAATTTAAAAAGGCTTTTCCCGGATCGCCCGTGGTGTGGGCCTATACATCACAGATCATT
CGTAAAAAACTGGCCGAACAGGGCCAGCCCGTGGGCGGCATCAGCGATGGCATGGCCCAGCTTGCCAAAGACGGCGTAAA
GGTTGTGCGCGTGCAGTCGCTGCATGTGCTTGGCGGCGAAGAATTTTCAGCCCTTGAACGCGCAGTTCTGCTTGACGTGA
GCAAACATCCGGGCCGTTTCGACGCGGTTTTCCTGGGCCGCCCCCTGCTGGAATCCAGGGCCGATGCGCAGGAACTGGCT
GCGATCCTGCTGGCTGACACCAAAAAAGCCCGCGGCAAAAATACTGCCCTTGTGCTTATGGGCCACGGCCAGAGCCACGG
GCGCGCCGACCTTGCCTTTGAAGGCGTATGCGCCGTGTTCAAGGCTGCGGACAAAAAGGTCCACATGGCCACAGTAGAGG
GCGCGCGCACCTTTGAAGACCTGCTCAAGGAACTCAAAGCCGCCAAGGTCAAGCAGGTGGTACTGGCCCCGCTGATGCTG
GTTGCCGGAGACCACGCCCGCAACGACCTTGCCGGCGACGAGGAAGACTCGTGGGCCAGCCAGCTCAAAAAAGCGGGCAT
CAAGGTTGACGCCCACCTCAAAGGCCTGGGCGAGATGCCCGGCACGGCCGCCATTTTCGTGCGCCATGCCAAGGAAAGTG
CCGACGACCTGACCAGGGAACCCAGAAAGCCGTAA

Upstream 100 bases:

>100_bases
CCAAGGCGCGGGCCATATGGCCCCACAGCCCTCAAGTCCGAACATCCGCCGGGGCCGGTGCCCCTTGCGAACCTTTCAAC
GCGAAATTGAGGAGGTTGCC

Downstream 100 bases:

>100_bases
CAGGCCGCATAAGCGCCTGCTGGCTGCCATCCCGCACGGGCGCGTTACAACAATGTCAGGTGCGCGGGCCGGGCCATGCC
CCGGGCCGCGCACCTTTTTT

Product: anaerobic cobalt chelatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MPRILSHAGFRPLLFVLLLLASLCLHGTSHAAGAPKEGVLLVAFGTSVPEGMPAFKAVDAEFKKAFPGSPVVWAYTSQII
RKKLAEQGQPVGGISDGMAQLAKDGVKVVRVQSLHVLGGEEFSALERAVLLDVSKHPGRFDAVFLGRPLLESRADAQELA
AILLADTKKARGKNTALVLMGHGQSHGRADLAFEGVCAVFKAADKKVHMATVEGARTFEDLLKELKAAKVKQVVLAPLML
VAGDHARNDLAGDEEDSWASQLKKAGIKVDAHLKGLGEMPGTAAIFVRHAKESADDLTREPRKP

Sequences:

>Translated_304_residues
MPRILSHAGFRPLLFVLLLLASLCLHGTSHAAGAPKEGVLLVAFGTSVPEGMPAFKAVDAEFKKAFPGSPVVWAYTSQII
RKKLAEQGQPVGGISDGMAQLAKDGVKVVRVQSLHVLGGEEFSALERAVLLDVSKHPGRFDAVFLGRPLLESRADAQELA
AILLADTKKARGKNTALVLMGHGQSHGRADLAFEGVCAVFKAADKKVHMATVEGARTFEDLLKELKAAKVKQVVLAPLML
VAGDHARNDLAGDEEDSWASQLKKAGIKVDAHLKGLGEMPGTAAIFVRHAKESADDLTREPRKP
>Mature_303_residues
PRILSHAGFRPLLFVLLLLASLCLHGTSHAAGAPKEGVLLVAFGTSVPEGMPAFKAVDAEFKKAFPGSPVVWAYTSQIIR
KKLAEQGQPVGGISDGMAQLAKDGVKVVRVQSLHVLGGEEFSALERAVLLDVSKHPGRFDAVFLGRPLLESRADAQELAA
ILLADTKKARGKNTALVLMGHGQSHGRADLAFEGVCAVFKAADKKVHMATVEGARTFEDLLKELKAAKVKQVVLAPLMLV
AGDHARNDLAGDEEDSWASQLKKAGIKVDAHLKGLGEMPGTAAIFVRHAKESADDLTREPRKP

Specific function: Chelates cobalt to the cobalamin precursor as part of the anaerobic pathway to cobalamin biosynthesis [H]

COG id: COG4822

COG function: function code H; Cobalamin biosynthesis protein CbiK, Co2+ chelatase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CbiK family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010388 [H]

Pfam domain/function: PF06180 CbiK [H]

EC number: =4.99.1.3 [H]

Molecular weight: Translated: 32379; Mature: 32248

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRILSHAGFRPLLFVLLLLASLCLHGTSHAAGAPKEGVLLVAFGTSVPEGMPAFKAVDA
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHH
EFKKAFPGSPVVWAYTSQIIRKKLAEQGQPVGGISDGMAQLAKDGVKVVRVQSLHVLGGE
HHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEECCC
EFSALERAVLLDVSKHPGRFDAVFLGRPLLESRADAQELAAILLADTKKARGKNTALVLM
HHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCHHHHHHHHHHHCHHCCCCCCEEEEE
GHGQSHGRADLAFEGVCAVFKAADKKVHMATVEGARTFEDLLKELKAAKVKQVVLAPLML
ECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAGDHARNDLAGDEEDSWASQLKKAGIKVDAHLKGLGEMPGTAAIFVRHAKESADDLTRE
HCCCCCCCCCCCCCCHHHHHHHHHCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
PRKP
CCCC
>Mature Secondary Structure 
PRILSHAGFRPLLFVLLLLASLCLHGTSHAAGAPKEGVLLVAFGTSVPEGMPAFKAVDA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHH
EFKKAFPGSPVVWAYTSQIIRKKLAEQGQPVGGISDGMAQLAKDGVKVVRVQSLHVLGGE
HHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEECCC
EFSALERAVLLDVSKHPGRFDAVFLGRPLLESRADAQELAAILLADTKKARGKNTALVLM
HHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCHHHHHHHHHHHCHHCCCCCCEEEEE
GHGQSHGRADLAFEGVCAVFKAADKKVHMATVEGARTFEDLLKELKAAKVKQVVLAPLML
ECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAGDHARNDLAGDEEDSWASQLKKAGIKVDAHLKGLGEMPGTAAIFVRHAKESADDLTRE
HCCCCCCCCCCCCCCHHHHHHHHHCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
PRKP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609; 9150215; 10451360 [H]