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The map label for this gene is pleD [H]

Identifier: 220905592

GI number: 220905592

Start: 2805877

End: 2806851

Strand: Reverse

Name: pleD [H]

Synonym: Ddes_2332

Alternate gene names: 220905592

Gene position: 2806851-2805877 (Counterclockwise)

Preceding gene: 220905593

Following gene: 220905586

Centisome position: 97.68

GC content: 56.92

Gene sequence:

>975_bases
ATGATGGGCCGTGTAAAAACTATGAGCAGCCTCAGTCTTGCCACATTGATGACCGTTCTGGACACGCTGCCCAATGCCGT
CTTTGTCAAGGGTGCCGACCTGCGTTTTACGTTCATCAACAGGGCATATGAAAAAATGTTCGGCGTGAGCAGAAAGGACG
TTCTGGGAAAAACTGTTCTGGACCTGGAATACCTGCCGGAAGAAGACAGGAAAATGTACCAGTCTGAAGACAGGGAGATG
GTCCGCCGGCAAGAGGTGAGCCACCACGTGCGCAGTTATCTGTTTGCAGACGGCAAGGAGCACACCTGTCTTTACTGGAG
CGGCGGCTTTGAAGACGAAAACGGCGTACGCGGCCTTTTGGGTGTCATTGTGGATATCGCGGAGCAAAGCCATACCATCG
GCGCGCTGCGCAGCCAGCTTGATTCTATGATCACCAGGGTCAGGCAGGCTGACGAAAGCAGCCTGACAGACGCGCTCACC
GGGCTGTATACCCGCAAATATCTGGATATGGTGCTGGGGCGGTACACCCGGCTGGAGAAAAAGCCTTTTTGCTGCATCAT
GCTGGATGTGGACCATTTCAAGCAGATCAACGACAGTTTCGGCCATCTTGTGGGCGACAGTGTGTTGAAAGACGTGGCGC
AGGTTATCCGTCGCTGTGTGCGTGAAATGGATACGGTATGTCGCTATGGCGGCGAAGAGTTTGTGGTGCTGATGCCCGGC
AGCCTTCTGGAACAGGCTGAACAGGCTGCGGAGCGGATTCGCAGGGCTGTTTCCGGCATGATAAGCGGGCCTGACAGCCG
GCCTGTGACCGTCAGTCTCGGGTGCAGCGAATACGAAATGGATGAAGACGCCTTGCAGGTTCTGCACCGGGCAGACAAGG
CCCTTTATGGCGCCAAGCGCTCCGGACGCAACCGGGTGTGGGTGGCGCGGCGCAATCCGGTTGCTGCCGTGCATGCCGGG
CCAGGTTGCCGCTGA

Upstream 100 bases:

>100_bases
AGAGGAAGCCATGTCGTTTACAGTCAGGAAAACCATATTGCTGATCCCTGTCACCTGCTAACGCCATTGCTGCGGCTTCG
GGCCTTTGGCCGCGCCGCGC

Downstream 100 bases:

>100_bases
CGCCCGGTTGCTGTGCGCGGTCCTGTTCTGCCCAGGCCGGTTCCCGCCCTGAAGAATGCCCCGGCAGAATTCTGTACGGG
CCTGGCATAAAAAACATGCC

Product: PAS/PAC sensor-containing diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MMGRVKTMSSLSLATLMTVLDTLPNAVFVKGADLRFTFINRAYEKMFGVSRKDVLGKTVLDLEYLPEEDRKMYQSEDREM
VRRQEVSHHVRSYLFADGKEHTCLYWSGGFEDENGVRGLLGVIVDIAEQSHTIGALRSQLDSMITRVRQADESSLTDALT
GLYTRKYLDMVLGRYTRLEKKPFCCIMLDVDHFKQINDSFGHLVGDSVLKDVAQVIRRCVREMDTVCRYGGEEFVVLMPG
SLLEQAEQAAERIRRAVSGMISGPDSRPVTVSLGCSEYEMDEDALQVLHRADKALYGAKRSGRNRVWVARRNPVAAVHAG
PGCR

Sequences:

>Translated_324_residues
MMGRVKTMSSLSLATLMTVLDTLPNAVFVKGADLRFTFINRAYEKMFGVSRKDVLGKTVLDLEYLPEEDRKMYQSEDREM
VRRQEVSHHVRSYLFADGKEHTCLYWSGGFEDENGVRGLLGVIVDIAEQSHTIGALRSQLDSMITRVRQADESSLTDALT
GLYTRKYLDMVLGRYTRLEKKPFCCIMLDVDHFKQINDSFGHLVGDSVLKDVAQVIRRCVREMDTVCRYGGEEFVVLMPG
SLLEQAEQAAERIRRAVSGMISGPDSRPVTVSLGCSEYEMDEDALQVLHRADKALYGAKRSGRNRVWVARRNPVAAVHAG
PGCR
>Mature_324_residues
MMGRVKTMSSLSLATLMTVLDTLPNAVFVKGADLRFTFINRAYEKMFGVSRKDVLGKTVLDLEYLPEEDRKMYQSEDREM
VRRQEVSHHVRSYLFADGKEHTCLYWSGGFEDENGVRGLLGVIVDIAEQSHTIGALRSQLDSMITRVRQADESSLTDALT
GLYTRKYLDMVLGRYTRLEKKPFCCIMLDVDHFKQINDSFGHLVGDSVLKDVAQVIRRCVREMDTVCRYGGEEFVVLMPG
SLLEQAEQAAERIRRAVSGMISGPDSRPVTVSLGCSEYEMDEDALQVLHRADKALYGAKRSGRNRVWVARRNPVAAVHAG
PGCR

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=40.9937888198758, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081881, Length=219, Percent_Identity=35.6164383561644, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787262, Length=158, Percent_Identity=38.6075949367089, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI145693134, Length=188, Percent_Identity=34.0425531914894, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787816, Length=155, Percent_Identity=37.4193548387097, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788381, Length=314, Percent_Identity=28.0254777070064, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786584, Length=168, Percent_Identity=32.7380952380952, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788085, Length=186, Percent_Identity=30.6451612903226, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787541, Length=170, Percent_Identity=32.3529411764706, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787802, Length=156, Percent_Identity=33.974358974359, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081974, Length=159, Percent_Identity=34.5911949685535, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787056, Length=166, Percent_Identity=33.7349397590361, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081977, Length=181, Percent_Identity=30.3867403314917, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 36527; Mature: 36527

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS50112 PAS ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGRVKTMSSLSLATLMTVLDTLPNAVFVKGADLRFTFINRAYEKMFGVSRKDVLGKTVL
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHHHCCCHHHHHHHHHH
DLEYLPEEDRKMYQSEDREMVRRQEVSHHVRSYLFADGKEHTCLYWSGGFEDENGVRGLL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHH
GVIVDIAEQSHTIGALRSQLDSMITRVRQADESSLTDALTGLYTRKYLDMVLGRYTRLEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KPFCCIMLDVDHFKQINDSFGHLVGDSVLKDVAQVIRRCVREMDTVCRYGGEEFVVLMPG
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
SLLEQAEQAAERIRRAVSGMISGPDSRPVTVSLGCSEYEMDEDALQVLHRADKALYGAKR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHC
SGRNRVWVARRNPVAAVHAGPGCR
CCCCEEEEEECCCEEEEECCCCCC
>Mature Secondary Structure
MMGRVKTMSSLSLATLMTVLDTLPNAVFVKGADLRFTFINRAYEKMFGVSRKDVLGKTVL
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHHHCCCHHHHHHHHHH
DLEYLPEEDRKMYQSEDREMVRRQEVSHHVRSYLFADGKEHTCLYWSGGFEDENGVRGLL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHH
GVIVDIAEQSHTIGALRSQLDSMITRVRQADESSLTDALTGLYTRKYLDMVLGRYTRLEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KPFCCIMLDVDHFKQINDSFGHLVGDSVLKDVAQVIRRCVREMDTVCRYGGEEFVVLMPG
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
SLLEQAEQAAERIRRAVSGMISGPDSRPVTVSLGCSEYEMDEDALQVLHRADKALYGAKR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHC
SGRNRVWVARRNPVAAVHAGPGCR
CCCCEEEEEECCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA