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The map label for this gene is metK [H]

Identifier: 220905548

GI number: 220905548

Start: 2753925

End: 2755097

Strand: Direct

Name: metK [H]

Synonym: Ddes_2287

Alternate gene names: 220905548

Gene position: 2753925-2755097 (Clockwise)

Preceding gene: 220905547

Following gene: 220905549

Centisome position: 95.84

GC content: 61.04

Gene sequence:

>1173_bases
ATGCAGACCAAGGGCAAATATTTTTTCACCTCTGAATCCGTTACCGAAGGCCACCCCGACAAGGTGGCCGACCAGATATC
AGACGCAGTCCTGGACACCCTGCTGGCGCAGGACCCGGACGCTCACGTGGCCTGCGAAGCACTGGTCACCACGGGCATGG
CTGTTATCGCCGGTGAAATCAGCACCACCGGCTATGCCGACCTGCCCACCGTGGTGCGCGAAACCATCAAAAACATCGGC
TACAACAGCTCCGAAATGGGCTTTGACTGGCAGACCTGCGCCGTCATTTCCACCATCGGCCATCAGTCCCCGGACATTGC
CCAGGGCGTGGTGCGCTCAAAGCCCGAAGATCAGGGTGCGGGCGACCAGGGCATGATGTTCGGCTTTGCCTGCAATGAAA
CCTCTACCCTCATGCCTGCCCCCATTTACTGGGCGCACCAGCTTTCACAGCAGCTTGCCAGGGTGCGCAAGGACGGCCTG
GTGGACTTTTTCCGCCCGGACGGCAAGACCCAGGTGTCTTTTGAATACCAGGACGGCAAGCCCGTGCGCATCAACAACGT
GGTGGTCTCCACCCAGCATGCTGCCGGCGCAAGCCAGGACGATGTGGCCGAAGCCGTGAAAAAACACGTCATCCGCCCCA
TTCTTGAGCCTTCGGGCTATTTCGACGAAAAAGACTGCGAAATCTTCATCAACACCACCGGACGGTTTGTGGTGGGTGGC
CCCATGGGCGACTGCGGGCTTACCGGCCGCAAGATCATCCAGGACACCTACGGCGGCAGCGGCCACCACGGCGGCGGCGC
GTTCTCCGGCAAGGACCCTTCCAAGGTGGACCGTTCCGGCGCATACATGGGCCGCTACATCGCCAAAAACGTGGTGGCCG
CTGGCCTTGCCCCGGTGTGCGAAGTGCAGATAGCCTACTGCATCGGTGTGGCCCAGCCCGTGAGCGTGCTTGTTTCCTCG
CAGGGAACCAGCGAAATTCCTGATGAAGTCCTCACCAAAGCCGTGCGCGAAGTTTTTGACCTGCGCCCCTACTTCATCAC
CAAGCGCCTTGATCTCAAGCGGCCCATCTACCAGAAGAGCGCCTGCTACGGCCACTTTGGCCGCGAGCTGCCCGAATTCA
CCTGGGAAGCCACCGATGCCGTGGCCGACCTGCGCACCGCCGCCAAGGTGTAA

Upstream 100 bases:

>100_bases
GGCCTGTGCCGTGCGTATGGGCAAGGCACGCCTTATCGACAACATTCTTTTACATTCGTAAACCACCGCGCCTGCCGCGC
GTCCCTTCAAGGAGAACAGC

Downstream 100 bases:

>100_bases
ACGGAAAAGCAGCAACGCTTCAGGTTGCGTTATCAAGAGTTGCAGTGAGCGCCCTGTGAACTTTGCGCATGCAGACGTTT
AAAACCAGCGTGCTCTGACT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MQTKGKYFFTSESVTEGHPDKVADQISDAVLDTLLAQDPDAHVACEALVTTGMAVIAGEISTTGYADLPTVVRETIKNIG
YNSSEMGFDWQTCAVISTIGHQSPDIAQGVVRSKPEDQGAGDQGMMFGFACNETSTLMPAPIYWAHQLSQQLARVRKDGL
VDFFRPDGKTQVSFEYQDGKPVRINNVVVSTQHAAGASQDDVAEAVKKHVIRPILEPSGYFDEKDCEIFINTTGRFVVGG
PMGDCGLTGRKIIQDTYGGSGHHGGGAFSGKDPSKVDRSGAYMGRYIAKNVVAAGLAPVCEVQIAYCIGVAQPVSVLVSS
QGTSEIPDEVLTKAVREVFDLRPYFITKRLDLKRPIYQKSACYGHFGRELPEFTWEATDAVADLRTAAKV

Sequences:

>Translated_390_residues
MQTKGKYFFTSESVTEGHPDKVADQISDAVLDTLLAQDPDAHVACEALVTTGMAVIAGEISTTGYADLPTVVRETIKNIG
YNSSEMGFDWQTCAVISTIGHQSPDIAQGVVRSKPEDQGAGDQGMMFGFACNETSTLMPAPIYWAHQLSQQLARVRKDGL
VDFFRPDGKTQVSFEYQDGKPVRINNVVVSTQHAAGASQDDVAEAVKKHVIRPILEPSGYFDEKDCEIFINTTGRFVVGG
PMGDCGLTGRKIIQDTYGGSGHHGGGAFSGKDPSKVDRSGAYMGRYIAKNVVAAGLAPVCEVQIAYCIGVAQPVSVLVSS
QGTSEIPDEVLTKAVREVFDLRPYFITKRLDLKRPIYQKSACYGHFGRELPEFTWEATDAVADLRTAAKV
>Mature_390_residues
MQTKGKYFFTSESVTEGHPDKVADQISDAVLDTLLAQDPDAHVACEALVTTGMAVIAGEISTTGYADLPTVVRETIKNIG
YNSSEMGFDWQTCAVISTIGHQSPDIAQGVVRSKPEDQGAGDQGMMFGFACNETSTLMPAPIYWAHQLSQQLARVRKDGL
VDFFRPDGKTQVSFEYQDGKPVRINNVVVSTQHAAGASQDDVAEAVKKHVIRPILEPSGYFDEKDCEIFINTTGRFVVGG
PMGDCGLTGRKIIQDTYGGSGHHGGGAFSGKDPSKVDRSGAYMGRYIAKNVVAAGLAPVCEVQIAYCIGVAQPVSVLVSS
QGTSEIPDEVLTKAVREVFDLRPYFITKRLDLKRPIYQKSACYGHFGRELPEFTWEATDAVADLRTAAKV

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=379, Percent_Identity=53.0343007915567, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI5174529, Length=378, Percent_Identity=51.5873015873016, Blast_Score=373, Evalue=1e-103,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=60.313315926893, Blast_Score=481, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17538498, Length=376, Percent_Identity=52.1276595744681, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17538494, Length=379, Percent_Identity=51.4511873350923, Blast_Score=366, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25145633, Length=395, Percent_Identity=50.6329113924051, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17551082, Length=381, Percent_Identity=50.6561679790026, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32565905, Length=379, Percent_Identity=47.2295514511873, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI32565911, Length=329, Percent_Identity=49.2401215805471, Blast_Score=298, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI25145635, Length=345, Percent_Identity=47.8260869565217, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI32565903, Length=319, Percent_Identity=48.58934169279, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI32565909, Length=86, Percent_Identity=55.8139534883721, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32565907, Length=43, Percent_Identity=72.0930232558139, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323209, Length=377, Percent_Identity=51.1936339522546, Blast_Score=361, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6320710, Length=381, Percent_Identity=49.8687664041995, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580527, Length=381, Percent_Identity=49.3438320209974, Blast_Score=345, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24580525, Length=381, Percent_Identity=49.3438320209974, Blast_Score=345, Evalue=2e-95,
Organism=Drosophila melanogaster, GI45552159, Length=381, Percent_Identity=49.0813648293963, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24580529, Length=381, Percent_Identity=49.0813648293963, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24580537, Length=381, Percent_Identity=49.0813648293963, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24580533, Length=381, Percent_Identity=49.0813648293963, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24580535, Length=381, Percent_Identity=49.0813648293963, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24580531, Length=381, Percent_Identity=49.0813648293963, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24580541, Length=362, Percent_Identity=48.8950276243094, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24580539, Length=124, Percent_Identity=53.2258064516129, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42145; Mature: 42145

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKGKYFFTSESVTEGHPDKVADQISDAVLDTLLAQDPDAHVACEALVTTGMAVIAGEI
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHEEECC
STTGYADLPTVVRETIKNIGYNSSEMGFDWQTCAVISTIGHQSPDIAQGVVRSKPEDQGA
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
GDQGMMFGFACNETSTLMPAPIYWAHQLSQQLARVRKDGLVDFFRPDGKTQVSFEYQDGK
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCC
PVRINNVVVSTQHAAGASQDDVAEAVKKHVIRPILEPSGYFDEKDCEIFINTTGRFVVGG
EEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEECC
PMGDCGLTGRKIIQDTYGGSGHHGGGAFSGKDPSKVDRSGAYMGRYIAKNVVAAGLAPVC
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EVQIAYCIGVAQPVSVLVSSQGTSEIPDEVLTKAVREVFDLRPYFITKRLDLKRPIYQKS
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHH
ACYGHFGRELPEFTWEATDAVADLRTAAKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTKGKYFFTSESVTEGHPDKVADQISDAVLDTLLAQDPDAHVACEALVTTGMAVIAGEI
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHEEECC
STTGYADLPTVVRETIKNIGYNSSEMGFDWQTCAVISTIGHQSPDIAQGVVRSKPEDQGA
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
GDQGMMFGFACNETSTLMPAPIYWAHQLSQQLARVRKDGLVDFFRPDGKTQVSFEYQDGK
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCC
PVRINNVVVSTQHAAGASQDDVAEAVKKHVIRPILEPSGYFDEKDCEIFINTTGRFVVGG
EEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEECC
PMGDCGLTGRKIIQDTYGGSGHHGGGAFSGKDPSKVDRSGAYMGRYIAKNVVAAGLAPVC
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EVQIAYCIGVAQPVSVLVSSQGTSEIPDEVLTKAVREVFDLRPYFITKRLDLKRPIYQKS
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHH
ACYGHFGRELPEFTWEATDAVADLRTAAKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA