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The map label for this gene is purA

Identifier: 220905527

GI number: 220905527

Start: 2726720

End: 2727997

Strand: Direct

Name: purA

Synonym: Ddes_2266

Alternate gene names: 220905527

Gene position: 2726720-2727997 (Clockwise)

Preceding gene: 220905526

Following gene: 220905528

Centisome position: 94.89

GC content: 60.72

Gene sequence:

>1278_bases
ATGGCGAATACGGTCATCATCGGCGCACAGTGGGGCGACGAAGGCAAAGGCAAAATCGTTGACATGCTGAGCGCCCAAAG
CCGGGCCATTGTCCGTTTTCAGGGCGGCAACAATGCCGGGCACACCATCAAGGTACAGGGCGAAGAAACCATCCTTCACC
TCATTCCTTCGGGCATACTGCACGCTGACAAGATGTGCCTCATCGGCAACGGTGTGGTGCTTGACCCGCACGTTTTTCTT
GAAGAAGTGGACCACCTGGCTGCCAAGGGCGTTGACGTTTCCGCGGCCAGGCTCGGCATCAGCAAAAAAGCCCACCTCAT
CATGCCTTACCACAAAAGCCTGGACAAAGCCCGCGAAGCCAAACGCGCTTCGCACAAAATAGGCACAACCGGGCGCGGCA
TCGGTCCCTGCTACGAAGACAAGGCCGCCCGTGTGGGCCTGCGCGCAGGCGATCTGGCCAATCCCGACCTGGTGCGGGCC
AAGGTGGCCCACGCCCTGCAGGAAAAAAACACCCTGCTGCGCGATCTGTACAAATTTGATCCGCTGGACGAAGCCGCCGT
ATGCCAAGAGCTGCTGGCCCTCGCCCCGCGACTGCTGCCATACCTTACGGAAGTGGAAGACCACATTCAGAAAATCCAGG
CCCAGGGCGGCGACATTCTTTTTGAAGGCGCGCAAGGCATTCACCTGGATATCGACCACGGCACATACCCCTTTGTCACG
TCCTCCAACACCGTGTCGGGCAATGCCTCAGCGGGCTGCGGCGTGGGTCCTTCTGCCCTGCACCGCGTGGTGGGCATCGT
AAAGGCCTATACCACCCGCGTGGGTTCCGGCCCCTTTCCCACCGAGCAGCTTGACGACACCGGCAGCTACCTGCGCACCC
AGGGACACGAATTCGGGGCCACCACGGGCCGCCCGCGCCGCTGCGGCTGGCTGGACGCCGTGGTACTGCGCGAGAGCGTG
CGCCTGTGCGGCATGACCGACATAGCCCTGACCAAGCTTGACGTGCTGCAGAACCTGCCCGGCCTTCGCATCTGCGTGGC
CTATGAATATGAAGGCCACAAGCTTGATTACATGCCGCAGGAAGAAGGCGCGCTTGAAAAGGTCACGCCCGTATATGAAG
ACCTGCCCGGCTTTGAGGAAGACATTACCGGATGCACCCGCTTTGATGACCTGCCCGAAACCGTACGGGCCTACATCGCG
CGTATTGAAGAGCTTGTCGGTGTAAAGGTGAGCATGGTTTCCGTAGGCGCCGAGCGCAGACAGACCATCGTGCGCTGA

Upstream 100 bases:

>100_bases
GGCAGTTTGACAAAGCGGTCATTTGTCGCGCCCGCGGGTGCGTAACGCTTCCTCATGACAGTACGCCTCCCCTGCGGCGG
CGTTTCAGGCAGGTAAAATC

Downstream 100 bases:

>100_bases
GGCGCAGTCGCGCCATGACCGCGCCCATGCTTCCGGCTATTGCTGTTCCGGCCTTTGACAGGCTCAAGGATGCGTTGAAC
CGCCTTGGGGCGGCCCCGCC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MANTVIIGAQWGDEGKGKIVDMLSAQSRAIVRFQGGNNAGHTIKVQGEETILHLIPSGILHADKMCLIGNGVVLDPHVFL
EEVDHLAAKGVDVSAARLGISKKAHLIMPYHKSLDKAREAKRASHKIGTTGRGIGPCYEDKAARVGLRAGDLANPDLVRA
KVAHALQEKNTLLRDLYKFDPLDEAAVCQELLALAPRLLPYLTEVEDHIQKIQAQGGDILFEGAQGIHLDIDHGTYPFVT
SSNTVSGNASAGCGVGPSALHRVVGIVKAYTTRVGSGPFPTEQLDDTGSYLRTQGHEFGATTGRPRRCGWLDAVVLRESV
RLCGMTDIALTKLDVLQNLPGLRICVAYEYEGHKLDYMPQEEGALEKVTPVYEDLPGFEEDITGCTRFDDLPETVRAYIA
RIEELVGVKVSMVSVGAERRQTIVR

Sequences:

>Translated_425_residues
MANTVIIGAQWGDEGKGKIVDMLSAQSRAIVRFQGGNNAGHTIKVQGEETILHLIPSGILHADKMCLIGNGVVLDPHVFL
EEVDHLAAKGVDVSAARLGISKKAHLIMPYHKSLDKAREAKRASHKIGTTGRGIGPCYEDKAARVGLRAGDLANPDLVRA
KVAHALQEKNTLLRDLYKFDPLDEAAVCQELLALAPRLLPYLTEVEDHIQKIQAQGGDILFEGAQGIHLDIDHGTYPFVT
SSNTVSGNASAGCGVGPSALHRVVGIVKAYTTRVGSGPFPTEQLDDTGSYLRTQGHEFGATTGRPRRCGWLDAVVLRESV
RLCGMTDIALTKLDVLQNLPGLRICVAYEYEGHKLDYMPQEEGALEKVTPVYEDLPGFEEDITGCTRFDDLPETVRAYIA
RIEELVGVKVSMVSVGAERRQTIVR
>Mature_424_residues
ANTVIIGAQWGDEGKGKIVDMLSAQSRAIVRFQGGNNAGHTIKVQGEETILHLIPSGILHADKMCLIGNGVVLDPHVFLE
EVDHLAAKGVDVSAARLGISKKAHLIMPYHKSLDKAREAKRASHKIGTTGRGIGPCYEDKAARVGLRAGDLANPDLVRAK
VAHALQEKNTLLRDLYKFDPLDEAAVCQELLALAPRLLPYLTEVEDHIQKIQAQGGDILFEGAQGIHLDIDHGTYPFVTS
SNTVSGNASAGCGVGPSALHRVVGIVKAYTTRVGSGPFPTEQLDDTGSYLRTQGHEFGATTGRPRRCGWLDAVVLRESVR
LCGMTDIALTKLDVLQNLPGLRICVAYEYEGHKLDYMPQEEGALEKVTPVYEDLPGFEEDITGCTRFDDLPETVRAYIAR
IEELVGVKVSMVSVGAERRQTIVR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=425, Percent_Identity=43.5294117647059, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI34577063, Length=430, Percent_Identity=41.6279069767442, Blast_Score=312, Evalue=5e-85,
Organism=Homo sapiens, GI40316944, Length=395, Percent_Identity=42.0253164556962, Blast_Score=301, Evalue=9e-82,
Organism=Escherichia coli, GI1790620, Length=423, Percent_Identity=47.5177304964539, Blast_Score=407, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=42.5581395348837, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=42.5581395348837, Blast_Score=322, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=43.4883720930233, Blast_Score=343, Evalue=2e-95,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=42.0427553444181, Blast_Score=318, Evalue=6e-87,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_DESDA (B8J4N1)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480839.1
- GeneID:   7285983
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_2266
- HOGENOM:   HBG658237
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46099; Mature: 45968

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTVIIGAQWGDEGKGKIVDMLSAQSRAIVRFQGGNNAGHTIKVQGEETILHLIPSGIL
CCCEEEEEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCCEEEHHCHHHH
HADKMCLIGNGVVLDPHVFLEEVDHLAAKGVDVSAARLGISKKAHLIMPYHKSLDKAREA
CCCCEEEECCCEEECHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEECHHHHHHHHHHH
KRASHKIGTTGRGIGPCYEDKAARVGLRAGDLANPDLVRAKVAHALQEKNTLLRDLYKFD
HHHHHCCCCCCCCCCCCHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PLDEAAVCQELLALAPRLLPYLTEVEDHIQKIQAQGGDILFEGAQGIHLDIDHGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCEEE
SSNTVSGNASAGCGVGPSALHRVVGIVKAYTTRVGSGPFPTEQLDDTGSYLRTQGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHCCCCCCC
TTGRPRRCGWLDAVVLRESVRLCGMTDIALTKLDVLQNLPGLRICVAYEYEGHKLDYMPQ
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCEECCCCC
EEGALEKVTPVYEDLPGFEEDITGCTRFDDLPETVRAYIARIEELVGVKVSMVSVGAERR
CCCCHHHHCHHHHHCCCCHHHHCCCHHCCCCHHHHHHHHHHHHHHHCCEEEEEHHCHHHH
QTIVR
HHHCC
>Mature Secondary Structure 
ANTVIIGAQWGDEGKGKIVDMLSAQSRAIVRFQGGNNAGHTIKVQGEETILHLIPSGIL
CCEEEEEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCCEEEHHCHHHH
HADKMCLIGNGVVLDPHVFLEEVDHLAAKGVDVSAARLGISKKAHLIMPYHKSLDKAREA
CCCCEEEECCCEEECHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEECHHHHHHHHHHH
KRASHKIGTTGRGIGPCYEDKAARVGLRAGDLANPDLVRAKVAHALQEKNTLLRDLYKFD
HHHHHCCCCCCCCCCCCHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PLDEAAVCQELLALAPRLLPYLTEVEDHIQKIQAQGGDILFEGAQGIHLDIDHGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCEEE
SSNTVSGNASAGCGVGPSALHRVVGIVKAYTTRVGSGPFPTEQLDDTGSYLRTQGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHCCCCCCC
TTGRPRRCGWLDAVVLRESVRLCGMTDIALTKLDVLQNLPGLRICVAYEYEGHKLDYMPQ
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCEECCCCC
EEGALEKVTPVYEDLPGFEEDITGCTRFDDLPETVRAYIARIEELVGVKVSMVSVGAERR
CCCCHHHHCHHHHHCCCCHHHHCCCHHCCCCHHHHHHHHHHHHHHHCCEEEEEHHCHHHH
QTIVR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA