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The map label for this gene is cobS [H]

Identifier: 220905519

GI number: 220905519

Start: 2717106

End: 2717903

Strand: Direct

Name: cobS [H]

Synonym: Ddes_2258

Alternate gene names: 220905519

Gene position: 2717106-2717903 (Clockwise)

Preceding gene: 220905518

Following gene: 220905520

Centisome position: 94.56

GC content: 64.54

Gene sequence:

>798_bases
TTGCACCCGCAAAAATGCCGCTTGTCCTGGCCAGGCCGTTGTTATGACGCCCTGGCCTTTTTAACCCGCCTTGTGCCTCC
CCGTCGTGACTGCACCGGGCACGACCTTGGCCGCTGCCTGCCTTTTTACGGTCTGGCCGGACTGTGTATTGGCCTCACGC
TGACCCTGCCCTGCGTTTCTGTATATTTTCTGCTGCGCCAGACCCCTGAGGCCAACCTTGTGCTGTGCGCCGCCCTTGCG
GCCTGGCTGTGGATGGGCCTCGAGGTATGGGCCACACGGGGCCTGCACTGGGACGGGCTTGCTGACCTTGGCGATGCCTG
CGGCAGCGGCGCGCCGGGCGAACACTTTTGGAGCATCCTGCGCGACAGCCGCCTGGGCGCGTTTGGTGCCCTGCACCTTT
TGCTGGCTTTTGGCGGCATGTGGCTGGCACTGTGCTGGCATATACATGCCGGACAATGGCTCTGCCTTGTGACAGCCCCT
GCCTGGGGCCGGGCCTGCGCGCTCTGGCTGGCCGCATCTGCCGAGCCGCGCGAACCGCAGTCACTGGGCGGTCTGACCTG
CGCCGGAGCCAGCCCCGCACTGGCGCGAGGCTACGCACTGGGCGCGCTGCTGCTGTTATGCCTGCTATTTTTTACGGGAC
AGATAACCTTGTGGCAAGCACCGTTGACGGTCTGCGGGCAGTATGTTCTCATCCGCCGCATGGCGGCTCTGGCCCGTGAC
CGGGGCGGCGTTAACGGCGATTTCCTGGGCGCAGCCATACAATGGGGCCAATTGTGGTTTCTGCTGGCAACAGTCTAG

Upstream 100 bases:

>100_bases
ATCAAGGTTCCTGAGCGTATCAATGTGGAATACGTTGACTTTTTCCACCACCTTTATGCTCTGGCCTTCAACCACCCCAT
GACCCGCTAGGGTTTTGGCC

Downstream 100 bases:

>100_bases
AAAACTCGTGCCGTAAACAGCCCTGACGGCTACCAACGTATTTTCCGTCACGAAGGTACCGCGCCTGCGCCTGCGAGCGC
CGGAATGGACGGCCTGAAAA

Product: cobalamin-5-phosphate synthase CobS

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MHPQKCRLSWPGRCYDALAFLTRLVPPRRDCTGHDLGRCLPFYGLAGLCIGLTLTLPCVSVYFLLRQTPEANLVLCAALA
AWLWMGLEVWATRGLHWDGLADLGDACGSGAPGEHFWSILRDSRLGAFGALHLLLAFGGMWLALCWHIHAGQWLCLVTAP
AWGRACALWLAASAEPREPQSLGGLTCAGASPALARGYALGALLLLCLLFFTGQITLWQAPLTVCGQYVLIRRMAALARD
RGGVNGDFLGAAIQWGQLWFLLATV

Sequences:

>Translated_265_residues
MHPQKCRLSWPGRCYDALAFLTRLVPPRRDCTGHDLGRCLPFYGLAGLCIGLTLTLPCVSVYFLLRQTPEANLVLCAALA
AWLWMGLEVWATRGLHWDGLADLGDACGSGAPGEHFWSILRDSRLGAFGALHLLLAFGGMWLALCWHIHAGQWLCLVTAP
AWGRACALWLAASAEPREPQSLGGLTCAGASPALARGYALGALLLLCLLFFTGQITLWQAPLTVCGQYVLIRRMAALARD
RGGVNGDFLGAAIQWGQLWFLLATV
>Mature_265_residues
MHPQKCRLSWPGRCYDALAFLTRLVPPRRDCTGHDLGRCLPFYGLAGLCIGLTLTLPCVSVYFLLRQTPEANLVLCAALA
AWLWMGLEVWATRGLHWDGLADLGDACGSGAPGEHFWSILRDSRLGAFGALHLLLAFGGMWLALCWHIHAGQWLCLVTAP
AWGRACALWLAASAEPREPQSLGGLTCAGASPALARGYALGALLLLCLLFFTGQITLWQAPLTVCGQYVLIRRMAALARD
RGGVNGDFLGAAIQWGQLWFLLATV

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=256, Percent_Identity=30.859375, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 28700; Mature: 28700

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPQKCRLSWPGRCYDALAFLTRLVPPRRDCTGHDLGRCLPFYGLAGLCIGLTLTLPCVS
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VYFLLRQTPEANLVLCAALAAWLWMGLEVWATRGLHWDGLADLGDACGSGAPGEHFWSIL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHH
RDSRLGAFGALHLLLAFGGMWLALCWHIHAGQWLCLVTAPAWGRACALWLAASAEPREPQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCH
SLGGLTCAGASPALARGYALGALLLLCLLFFTGQITLWQAPLTVCGQYVLIRRMAALARD
HCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHH
RGGVNGDFLGAAIQWGQLWFLLATV
CCCCCCHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MHPQKCRLSWPGRCYDALAFLTRLVPPRRDCTGHDLGRCLPFYGLAGLCIGLTLTLPCVS
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VYFLLRQTPEANLVLCAALAAWLWMGLEVWATRGLHWDGLADLGDACGSGAPGEHFWSIL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHH
RDSRLGAFGALHLLLAFGGMWLALCWHIHAGQWLCLVTAPAWGRACALWLAASAEPREPQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCH
SLGGLTCAGASPALARGYALGALLLLCLLFFTGQITLWQAPLTVCGQYVLIRRMAALARD
HCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHH
RGGVNGDFLGAAIQWGQLWFLLATV
CCCCCCHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA