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The map label for this gene is gatA

Identifier: 220905416

GI number: 220905416

Start: 2597673

End: 2599133

Strand: Direct

Name: gatA

Synonym: Ddes_2154

Alternate gene names: 220905416

Gene position: 2597673-2599133 (Clockwise)

Preceding gene: 220905415

Following gene: 220905419

Centisome position: 90.4

GC content: 63.45

Gene sequence:

>1461_bases
ATGACCGACATATGCTCCCTCTCTCTCACGGCTGTGGCCCAGGCCCTGCAAAAAAAAGAACTGAGCGCGGTGGATGTCAC
CACGGCCTGCCTTGACCGCATCACCGCCACAGAGCCGCATCTGGCCGCCCTGCTTTACGTGAATGCCGAAAACGCGCTGG
CCCGTGCCACCACGCTGGACAGCGAAGGCCCCGATGCCGCACGGCCGCTGTGGGGTGTACCTGTCACGCTCAAGGACGCC
TTTTCCACCAGGGGCATGCCCACCACGGCCGGTTCGCGCATGCTTGAAGGCTATACGCCCTTCTACGATGCCTTTGCCGT
GCAAAAACTGCACGAAGCCGGGGCCGTCATTCTGGGCAAAACAAACCTGGACGAGTTCGCCATGGGCTCCAGCACGGAAA
ACTCGGCCTTCAAGGTCACACGTAATCCCTGGAACCTCAACAAGGTTCCGGGCGGGTCCAGCGGCGGCTCCGCCGCGTCG
GTAACAGCCGGACAGTGCTTCGCTTCTCTGGGAACCGACACCGGCGGCTCCATACGCCAGCCTGCGTCCTTCTGCGGCTG
CGTGGGCCTCAAGCCCACATATGGGCGGGTTTCGCGCTACGGGGTCATCGCCTATGGCTCGTCCCTTGATCAGGTCGGCC
CCCTCACACGTAGTGTGGAAGACTGCGCCCGCGTACTGACGGCCATTGCCGGATATGATTCACGCGACAATACCTGCGAT
CCGCGCCCGGCAGAAGACTATGCCGCGACTCTGTCTTCGCGGCCCCTCAAGGGCGCGCGCCTGGGCATTCCCAGGGAATT
TTACGGCCAGGGGCTGAGCGACGAGGTGCGGGCAGCCTGCGAGGCCGCCATACAGGCCGCCAGAAACCAGGACGCGGAAC
TGGTGGAAGTAAGCCTGCCCCACACGGATGCGGCCATTGCCACCTACTACATTATCGCCATGGCCGAAGCCAGCTCAAAC
CTGGCCCGCTTTGACGGCGTACGCTTCGGCCACCGCTCGGCAGACATCAAAAACCTTGACGATCTCTATGTACACAGCCG
CACCGAGGGCTTCGGGCCGGAAGTCAAGCGCCGCATCATGCTTGGGGCCTACGTGCTGTCATCCGGCTATTACGATGCCT
ACTACCGCAAGGCCGCCCAGGTGCGCCGTCTTATCCGTGATGAACTGCTCGCGGCTCTCGGCCAGTGCGATGCCCTGCTG
GCTCCCGTATCGCCCGTGACCGCGCGCGACCTGGGCGGCAACACCGCTGATCCCCTGCAGATTTACCTTATGGACGCCTA
TACCCTGTCCCTGAACCTGGCCGGCCTGCCGGGCCTCTCCTTGCCTGTGGGCCTTGGGGCAAAAAGCGGCATGCCCGTGG
GCATGCAGATCATTGGCAAGCCTTTTGAAGAAACGCAGGTGCTGGCCCTTGGGCACAGCCTGGAGCAGGCACTGCCCGGC
ATCGGCAGGCCTGCGCTGTAA

Upstream 100 bases:

>100_bases
TGCGAATGCGGCACAGGCGACGGGCAAAACAGTGCGAGGCAGATCCTGCGGGCAGGTTTCCGTTTACGCCAACACTACAA
GCAGAACTTTCGAGAATACC

Downstream 100 bases:

>100_bases
CAACAGTGCGGTCCGGCCTGGTCTGGCACGGCAGGCAGAAATGCTCACACACGGGCACAGCGAGACGCCTCTGGCAAAAA
CCCGTTTACGAACCTGCCGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTDICSLSLTAVAQALQKKELSAVDVTTACLDRITATEPHLAALLYVNAENALARATTLDSEGPDAARPLWGVPVTLKDA
FSTRGMPTTAGSRMLEGYTPFYDAFAVQKLHEAGAVILGKTNLDEFAMGSSTENSAFKVTRNPWNLNKVPGGSSGGSAAS
VTAGQCFASLGTDTGGSIRQPASFCGCVGLKPTYGRVSRYGVIAYGSSLDQVGPLTRSVEDCARVLTAIAGYDSRDNTCD
PRPAEDYAATLSSRPLKGARLGIPREFYGQGLSDEVRAACEAAIQAARNQDAELVEVSLPHTDAAIATYYIIAMAEASSN
LARFDGVRFGHRSADIKNLDDLYVHSRTEGFGPEVKRRIMLGAYVLSSGYYDAYYRKAAQVRRLIRDELLAALGQCDALL
APVSPVTARDLGGNTADPLQIYLMDAYTLSLNLAGLPGLSLPVGLGAKSGMPVGMQIIGKPFEETQVLALGHSLEQALPG
IGRPAL

Sequences:

>Translated_486_residues
MTDICSLSLTAVAQALQKKELSAVDVTTACLDRITATEPHLAALLYVNAENALARATTLDSEGPDAARPLWGVPVTLKDA
FSTRGMPTTAGSRMLEGYTPFYDAFAVQKLHEAGAVILGKTNLDEFAMGSSTENSAFKVTRNPWNLNKVPGGSSGGSAAS
VTAGQCFASLGTDTGGSIRQPASFCGCVGLKPTYGRVSRYGVIAYGSSLDQVGPLTRSVEDCARVLTAIAGYDSRDNTCD
PRPAEDYAATLSSRPLKGARLGIPREFYGQGLSDEVRAACEAAIQAARNQDAELVEVSLPHTDAAIATYYIIAMAEASSN
LARFDGVRFGHRSADIKNLDDLYVHSRTEGFGPEVKRRIMLGAYVLSSGYYDAYYRKAAQVRRLIRDELLAALGQCDALL
APVSPVTARDLGGNTADPLQIYLMDAYTLSLNLAGLPGLSLPVGLGAKSGMPVGMQIIGKPFEETQVLALGHSLEQALPG
IGRPAL
>Mature_485_residues
TDICSLSLTAVAQALQKKELSAVDVTTACLDRITATEPHLAALLYVNAENALARATTLDSEGPDAARPLWGVPVTLKDAF
STRGMPTTAGSRMLEGYTPFYDAFAVQKLHEAGAVILGKTNLDEFAMGSSTENSAFKVTRNPWNLNKVPGGSSGGSAASV
TAGQCFASLGTDTGGSIRQPASFCGCVGLKPTYGRVSRYGVIAYGSSLDQVGPLTRSVEDCARVLTAIAGYDSRDNTCDP
RPAEDYAATLSSRPLKGARLGIPREFYGQGLSDEVRAACEAAIQAARNQDAELVEVSLPHTDAAIATYYIIAMAEASSNL
ARFDGVRFGHRSADIKNLDDLYVHSRTEGFGPEVKRRIMLGAYVLSSGYYDAYYRKAAQVRRLIRDELLAALGQCDALLA
PVSPVTARDLGGNTADPLQIYLMDAYTLSLNLAGLPGLSLPVGLGAKSGMPVGMQIIGKPFEETQVLALGHSLEQALPGI
GRPAL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=499, Percent_Identity=37.0741482965932, Blast_Score=305, Evalue=4e-83,
Organism=Homo sapiens, GI195972892, Length=502, Percent_Identity=26.6932270916335, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI166795287, Length=211, Percent_Identity=40.2843601895735, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=418, Percent_Identity=36.6028708133971, Blast_Score=254, Evalue=7e-68,
Organism=Caenorhabditis elegans, GI17537465, Length=503, Percent_Identity=25.4473161033797, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17556264, Length=233, Percent_Identity=31.7596566523605, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=182, Percent_Identity=34.6153846153846, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=251, Percent_Identity=29.4820717131474, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17556278, Length=308, Percent_Identity=29.5454545454545, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=308, Percent_Identity=29.5454545454545, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=202, Percent_Identity=28.2178217821782, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=427, Percent_Identity=35.8313817330211, Blast_Score=219, Evalue=9e-58,
Organism=Saccharomyces cerevisiae, GI6319685, Length=449, Percent_Identity=29.6213808463252, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6320448, Length=548, Percent_Identity=25.1824817518248, Blast_Score=113, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24648113, Length=420, Percent_Identity=41.1904761904762, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24652985, Length=510, Percent_Identity=27.6470588235294, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI19922090, Length=510, Percent_Identity=27.6470588235294, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24652981, Length=510, Percent_Identity=27.6470588235294, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24652983, Length=510, Percent_Identity=27.6470588235294, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24648435, Length=509, Percent_Identity=24.75442043222, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648437, Length=509, Percent_Identity=24.75442043222, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45550774, Length=509, Percent_Identity=24.75442043222, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078093, Length=521, Percent_Identity=26.8714011516315, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21356731, Length=510, Percent_Identity=25.4901960784314, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24644968, Length=505, Percent_Identity=24.3564356435644, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24648441, Length=450, Percent_Identity=25.5555555555556, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648439, Length=450, Percent_Identity=25.5555555555556, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_DESDA (B8J405)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480728.1
- GeneID:   7285870
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_2154
- HOGENOM:   HBG481888
- ProtClustDB:   PRK00012
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51369; Mature: 51238

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 153-153 ACT_SITE 177-177

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDICSLSLTAVAQALQKKELSAVDVTTACLDRITATEPHLAALLYVNAENALARATTLD
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHEECC
SEGPDAARPLWGVPVTLKDAFSTRGMPTTAGSRMLEGYTPFYDAFAVQKLHEAGAVILGK
CCCCCCCCCCCCCCEEHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCEEEEEC
TNLDEFAMGSSTENSAFKVTRNPWNLNKVPGGSSGGSAASVTAGQCFASLGTDTGGSIRQ
CCCHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCC
PASFCGCVGLKPTYGRVSRYGVIAYGSSLDQVGPLTRSVEDCARVLTAIAGYDSRDNTCD
CHHHHCCCCCCCCCCCHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PRPAEDYAATLSSRPLKGARLGIPREFYGQGLSDEVRAACEAAIQAARNQDAELVEVSLP
CCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECC
HTDAAIATYYIIAMAEASSNLARFDGVRFGHRSADIKNLDDLYVHSRTEGFGPEVKRRIM
CCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCHHHHEECCCCCCCHHHHHHHH
LGAYVLSSGYYDAYYRKAAQVRRLIRDELLAALGQCDALLAPVSPVTARDLGGNTADPLQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHCCCCCCCCEE
IYLMDAYTLSLNLAGLPGLSLPVGLGAKSGMPVGMQIIGKPFEETQVLALGHSLEQALPG
EEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCC
IGRPAL
CCCCCC
>Mature Secondary Structure 
TDICSLSLTAVAQALQKKELSAVDVTTACLDRITATEPHLAALLYVNAENALARATTLD
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHEECC
SEGPDAARPLWGVPVTLKDAFSTRGMPTTAGSRMLEGYTPFYDAFAVQKLHEAGAVILGK
CCCCCCCCCCCCCCEEHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCEEEEEC
TNLDEFAMGSSTENSAFKVTRNPWNLNKVPGGSSGGSAASVTAGQCFASLGTDTGGSIRQ
CCCHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCC
PASFCGCVGLKPTYGRVSRYGVIAYGSSLDQVGPLTRSVEDCARVLTAIAGYDSRDNTCD
CHHHHCCCCCCCCCCCHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PRPAEDYAATLSSRPLKGARLGIPREFYGQGLSDEVRAACEAAIQAARNQDAELVEVSLP
CCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECC
HTDAAIATYYIIAMAEASSNLARFDGVRFGHRSADIKNLDDLYVHSRTEGFGPEVKRRIM
CCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCHHHHEECCCCCCCHHHHHHHH
LGAYVLSSGYYDAYYRKAAQVRRLIRDELLAALGQCDALLAPVSPVTARDLGGNTADPLQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHCCCCCCCCEE
IYLMDAYTLSLNLAGLPGLSLPVGLGAKSGMPVGMQIIGKPFEETQVLALGHSLEQALPG
EEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCC
IGRPAL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA