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The map label for this gene is ilvE [H]

Identifier: 220905405

GI number: 220905405

Start: 2583877

End: 2584797

Strand: Direct

Name: ilvE [H]

Synonym: Ddes_2143

Alternate gene names: 220905405

Gene position: 2583877-2584797 (Clockwise)

Preceding gene: 220905396

Following gene: 220905406

Centisome position: 89.92

GC content: 57.33

Gene sequence:

>921_bases
ATGCAAAAGATGAAGTACATCTGGGTTGACGGAAAAATGGTTCCCTGGGACGAAGCCCAGGTTCACGTGCTCACCCATGC
CCTGCATTACGGCAGCGCCATTTTCGAGGGCATCCGGGCTTATGCCTGTGCCGACGGCACGTCCGCCATATTCCGCCTTG
AAGACCACTGCAAGCGTATGCTCAACTCCGCCAAGATCCTACGCATGAACCTCACTCTTACCGTAGAAGAGCTGGTAAAA
GCCTGCGTGGAAACGCTTGAAATCAACAAACTGGCCGAAGGCTATGTGCGTCCGCTTTCTTTCGTGGGCTATGGCGAAAT
GGGCGTCTACCCCGGCAACAATCCGGTGCAGACCATTGTAGCCACCTGGCCCTGGGGGGCCTATCTTGGCGCCGAAGCTC
TGGAAAAGGGCATTCGCGTCAAAACCAGCACCTTTGCCCGCAGCCATGTGAACACCAGCATGTCCAAGGCCAAGGCCGCA
GGCAACTACATCAACTCCATCCTTGCCAAGGTGGAAGCCAAGGACGAAGGCTATGACGAGGCCGTCATGCTGGACACCAG
CGGCTATGTGTCTGAAGCCACTGGTGAAAATATCTTTATCGTACGCGACGGCATCATCAAGACCACGCCCTGGACCTCCA
TCCTCGGCGGCATCACCCGTGACTCCGTCATGAAGCTGGCCCAGGACCTGGGCTACACGGTGGAAGAACAGCAGTTCACC
CGTGACGAAATGTATATTGCCGACGAGGCGTTCTTTACCGGCACCGCGGCGGAAATAACCCCCATCCGCGAGATGGACCA
CCGCCAGATCGGCGCCGGACACGCCGGGCCTGTGACCAAGCACCTGCAAACCGAGTACTTCAAGATCGTCAAGGGCCAGA
ACCCCAAGTACGCCGGCTGGCTGCACCACTATAAAGTCTAG

Upstream 100 bases:

>100_bases
ATATGGGGCGCTTTACAGTAAATTTTTTCTCTTGCGGACTCGACGGCCATGTGTGCCGCCAGTGATGCCGCCCTACTTAT
GAGTCTTGCGGAGGACAGTC

Downstream 100 bases:

>100_bases
CTCAACAGGCGCGCCCGGCCCGCCGGGCGCGCCGTCAGAAAATCATGAAGCATCTCTCCCTCGCCTCGCGCTACCGCCCC
CAGACCTTTGCCCAGGTGGC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MQKMKYIWVDGKMVPWDEAQVHVLTHALHYGSAIFEGIRAYACADGTSAIFRLEDHCKRMLNSAKILRMNLTLTVEELVK
ACVETLEINKLAEGYVRPLSFVGYGEMGVYPGNNPVQTIVATWPWGAYLGAEALEKGIRVKTSTFARSHVNTSMSKAKAA
GNYINSILAKVEAKDEGYDEAVMLDTSGYVSEATGENIFIVRDGIIKTTPWTSILGGITRDSVMKLAQDLGYTVEEQQFT
RDEMYIADEAFFTGTAAEITPIREMDHRQIGAGHAGPVTKHLQTEYFKIVKGQNPKYAGWLHHYKV

Sequences:

>Translated_306_residues
MQKMKYIWVDGKMVPWDEAQVHVLTHALHYGSAIFEGIRAYACADGTSAIFRLEDHCKRMLNSAKILRMNLTLTVEELVK
ACVETLEINKLAEGYVRPLSFVGYGEMGVYPGNNPVQTIVATWPWGAYLGAEALEKGIRVKTSTFARSHVNTSMSKAKAA
GNYINSILAKVEAKDEGYDEAVMLDTSGYVSEATGENIFIVRDGIIKTTPWTSILGGITRDSVMKLAQDLGYTVEEQQFT
RDEMYIADEAFFTGTAAEITPIREMDHRQIGAGHAGPVTKHLQTEYFKIVKGQNPKYAGWLHHYKV
>Mature_306_residues
MQKMKYIWVDGKMVPWDEAQVHVLTHALHYGSAIFEGIRAYACADGTSAIFRLEDHCKRMLNSAKILRMNLTLTVEELVK
ACVETLEINKLAEGYVRPLSFVGYGEMGVYPGNNPVQTIVATWPWGAYLGAEALEKGIRVKTSTFARSHVNTSMSKAKAA
GNYINSILAKVEAKDEGYDEAVMLDTSGYVSEATGENIFIVRDGIIKTTPWTSILGGITRDSVMKLAQDLGYTVEEQQFT
RDEMYIADEAFFTGTAAEITPIREMDHRQIGAGHAGPVTKHLQTEYFKIVKGQNPKYAGWLHHYKV

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=285, Percent_Identity=26.6666666666667, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI38176287, Length=287, Percent_Identity=25.4355400696864, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI296010904, Length=287, Percent_Identity=25.4355400696864, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI296010906, Length=287, Percent_Identity=25.4355400696864, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI48994963, Length=302, Percent_Identity=48.0132450331126, Blast_Score=303, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI17565728, Length=284, Percent_Identity=28.169014084507, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6322002, Length=288, Percent_Identity=25.6944444444444, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6322608, Length=283, Percent_Identity=25.7950530035336, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34096; Mature: 34096

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKMKYIWVDGKMVPWDEAQVHVLTHALHYGSAIFEGIRAYACADGTSAIFRLEDHCKRM
CCCCEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEHHHHHHHH
LNSAKILRMNLTLTVEELVKACVETLEINKLAEGYVRPLSFVGYGEMGVYPGNNPVQTIV
HCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCCCEEEE
ATWPWGAYLGAEALEKGIRVKTSTFARSHVNTSMSKAKAAGNYINSILAKVEAKDEGYDE
EECCCHHHHCHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AVMLDTSGYVSEATGENIFIVRDGIIKTTPWTSILGGITRDSVMKLAQDLGYTVEEQQFT
EEEEECCCCEECCCCCEEEEEECCEEECCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHHC
RDEMYIADEAFFTGTAAEITPIREMDHRQIGAGHAGPVTKHLQTEYFKIVKGQNPKYAGW
HHHEEEECCCEECCCCCCCCCHHHHCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
LHHYKV
HHCCCC
>Mature Secondary Structure
MQKMKYIWVDGKMVPWDEAQVHVLTHALHYGSAIFEGIRAYACADGTSAIFRLEDHCKRM
CCCCEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEHHHHHHHH
LNSAKILRMNLTLTVEELVKACVETLEINKLAEGYVRPLSFVGYGEMGVYPGNNPVQTIV
HCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCCCEEEE
ATWPWGAYLGAEALEKGIRVKTSTFARSHVNTSMSKAKAAGNYINSILAKVEAKDEGYDE
EECCCHHHHCHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AVMLDTSGYVSEATGENIFIVRDGIIKTTPWTSILGGITRDSVMKLAQDLGYTVEEQQFT
EEEEECCCCEECCCCCEEEEEECCEEECCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHHC
RDEMYIADEAFFTGTAAEITPIREMDHRQIGAGHAGPVTKHLQTEYFKIVKGQNPKYAGW
HHHEEEECCCEECCCCCCCCCHHHHCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
LHHYKV
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]