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The map label for this gene is braC [H]

Identifier: 220905376

GI number: 220905376

Start: 2549493

End: 2550710

Strand: Direct

Name: braC [H]

Synonym: Ddes_2114

Alternate gene names: 220905376

Gene position: 2549493-2550710 (Clockwise)

Preceding gene: 220905375

Following gene: 220905377

Centisome position: 88.73

GC content: 55.5

Gene sequence:

>1218_bases
ATGGGGCAAGCCCCTTCAATCCTGCACGTAAACCACCGGGAGGCTTTCATGAAGTTGTTCAAGTTGGTGACCGCCTGCGC
CTTGTCGCTTTTTGTGGGACAGGCGGCCCTTGCGGCAGAAAAGCCCATCAAAATCGGCTTCCCCATCCCCCTTACGGGCG
AAATCCCCAAGGTGGGCGAAGGCTCCAAGTTTGCGGCCGAAATGCTGAAAGAAGAAATCAACGCCAAGGGCGGACTCAAG
GTGGGCGATAAATATTACCCCCTCGAATTCATTTATGAAGACAACGAATCCAAGCCCGAATCCGCGGTCAATGTCACCCT
CAAGCTCATCGCGCGCGACAAGGTGCACGCCATTGTAGGCCCGCAGTCCTCACGTCAGGCCGTGCCTGCCGGGGGCGTCG
CCAATGATGAAGAAGTCCCCCTGATCACCCCCTGGTCCACCAATCCCGACGCCACCAACGGCCGCCCCTGGGTTTTCCGC
GGCGCGTTCCTTGATCCTTTCCAGGCGCCGGTGGCTGTAGACTTTGCCACCAAGCAGTTCAACGCCAAAAAGGCCGCCGT
GCTTTTCGAAGTCTCCAACGATTACTCCAAGGGCCTTGCCGACAATTTTAAAGAGGCTTTTGAAAAAACACACGGTAAAG
GCTCTGTAGTAGCCATGGAATCCCACGGTCCCAAGGACCAGGATTTTTCCGCCCAGTTGACCAAGATCATCGCAGCCAAG
CCCGACTTTATCTTTGTGCCCGAAAATTACAGCTTTGCGGCCCTGATCGTGCCGCAGGCCCGCGACCTTGGCTATAAGGG
ACCTTTCATGGGTTCTGATGCCTGGGGTTCGGCAGAGCTGTTCAACCTTTGCGGCAAAGACTGCGTGGGACAGTATTTCT
CCACCCACTATACGGCCGAAGGCGCAACGGGAAAAACGCGTGAATTCATCGACAAGTACAAGGCCAAGTACGGCTATGTG
CCTGACGATGTGGCCGCCCTTACCTGGGACTCCATCAACATCGTTCTTCAGGCCATACAAAATGCCGGCAAGGTTGATCC
GGACCTCAAAAAGCAGCGCAAGATCATCCGCGACAACATGGCTGCCATTGAAAACTTTGACGGCATCACCGGCAGCATGA
AATTTGACGACAACGGCGACCCCATAAAGTGCGCGGTTATCGTCAAGGTAACTGACAGCGGCAGCTTCGCCTTTGTGGAG
TCCGTCTGTCCCAAATAG

Upstream 100 bases:

>100_bases
AATTTGCCCCACGCTAGCTTTTTTACAAACCATGATGTAAATATATCTATCATTTCAGTCAGATCCCCGCTGTGGAACTT
TTCTTGCTTAAGGAGCCTGC

Downstream 100 bases:

>100_bases
CCAGACCTGCTGACAAGCCTGGGATGGCAGGCCGTGAGCGCCCGCGGGGGGCGGGCGCTCGCGGCACACACTGCCCGAGG
GCCTCTACGGCACGGAAAGC

Product: Extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MGQAPSILHVNHREAFMKLFKLVTACALSLFVGQAALAAEKPIKIGFPIPLTGEIPKVGEGSKFAAEMLKEEINAKGGLK
VGDKYYPLEFIYEDNESKPESAVNVTLKLIARDKVHAIVGPQSSRQAVPAGGVANDEEVPLITPWSTNPDATNGRPWVFR
GAFLDPFQAPVAVDFATKQFNAKKAAVLFEVSNDYSKGLADNFKEAFEKTHGKGSVVAMESHGPKDQDFSAQLTKIIAAK
PDFIFVPENYSFAALIVPQARDLGYKGPFMGSDAWGSAELFNLCGKDCVGQYFSTHYTAEGATGKTREFIDKYKAKYGYV
PDDVAALTWDSINIVLQAIQNAGKVDPDLKKQRKIIRDNMAAIENFDGITGSMKFDDNGDPIKCAVIVKVTDSGSFAFVE
SVCPK

Sequences:

>Translated_405_residues
MGQAPSILHVNHREAFMKLFKLVTACALSLFVGQAALAAEKPIKIGFPIPLTGEIPKVGEGSKFAAEMLKEEINAKGGLK
VGDKYYPLEFIYEDNESKPESAVNVTLKLIARDKVHAIVGPQSSRQAVPAGGVANDEEVPLITPWSTNPDATNGRPWVFR
GAFLDPFQAPVAVDFATKQFNAKKAAVLFEVSNDYSKGLADNFKEAFEKTHGKGSVVAMESHGPKDQDFSAQLTKIIAAK
PDFIFVPENYSFAALIVPQARDLGYKGPFMGSDAWGSAELFNLCGKDCVGQYFSTHYTAEGATGKTREFIDKYKAKYGYV
PDDVAALTWDSINIVLQAIQNAGKVDPDLKKQRKIIRDNMAAIENFDGITGSMKFDDNGDPIKCAVIVKVTDSGSFAFVE
SVCPK
>Mature_404_residues
GQAPSILHVNHREAFMKLFKLVTACALSLFVGQAALAAEKPIKIGFPIPLTGEIPKVGEGSKFAAEMLKEEINAKGGLKV
GDKYYPLEFIYEDNESKPESAVNVTLKLIARDKVHAIVGPQSSRQAVPAGGVANDEEVPLITPWSTNPDATNGRPWVFRG
AFLDPFQAPVAVDFATKQFNAKKAAVLFEVSNDYSKGLADNFKEAFEKTHGKGSVVAMESHGPKDQDFSAQLTKIIAAKP
DFIFVPENYSFAALIVPQARDLGYKGPFMGSDAWGSAELFNLCGKDCVGQYFSTHYTAEGATGKTREFIDKYKAKYGYVP
DDVAALTWDSINIVLQAIQNAGKVDPDLKKQRKIIRDNMAAIENFDGITGSMKFDDNGDPIKCAVIVKVTDSGSFAFVES
VCPK

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=363, Percent_Identity=25.6198347107438, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI48994941, Length=361, Percent_Identity=26.0387811634349, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 44007; Mature: 43876

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQAPSILHVNHREAFMKLFKLVTACALSLFVGQAALAAEKPIKIGFPIPLTGEIPKVGE
CCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
GSKFAAEMLKEEINAKGGLKVGDKYYPLEFIYEDNESKPESAVNVTLKLIARDKVHAIVG
CHHHHHHHHHHHHCCCCCCEECCEECCEEEEECCCCCCCCHHHEEEEEEEECCCEEEEEC
PQSSRQAVPAGGVANDEEVPLITPWSTNPDATNGRPWVFRGAFLDPFQAPVAVDFATKQF
CCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEHHHHC
NAKKAAVLFEVSNDYSKGLADNFKEAFEKTHGKGSVVAMESHGPKDQDFSAQLTKIIAAK
CCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHCC
PDFIFVPENYSFAALIVPQARDLGYKGPFMGSDAWGSAELFNLCGKDCVGQYFSTHYTAE
CCEEEECCCCCEEEEEECCHHCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHEEECC
GATGKTREFIDKYKAKYGYVPDDVAALTWDSINIVLQAIQNAGKVDPDLKKQRKIIRDNM
CCCCCHHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AAIENFDGITGSMKFDDNGDPIKCAVIVKVTDSGSFAFVESVCPK
HHHHCCCCCCEEEEECCCCCCEEEEEEEEEECCCCEEEHHHCCCC
>Mature Secondary Structure 
GQAPSILHVNHREAFMKLFKLVTACALSLFVGQAALAAEKPIKIGFPIPLTGEIPKVGE
CCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
GSKFAAEMLKEEINAKGGLKVGDKYYPLEFIYEDNESKPESAVNVTLKLIARDKVHAIVG
CHHHHHHHHHHHHCCCCCCEECCEECCEEEEECCCCCCCCHHHEEEEEEEECCCEEEEEC
PQSSRQAVPAGGVANDEEVPLITPWSTNPDATNGRPWVFRGAFLDPFQAPVAVDFATKQF
CCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEHHHHC
NAKKAAVLFEVSNDYSKGLADNFKEAFEKTHGKGSVVAMESHGPKDQDFSAQLTKIIAAK
CCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHCC
PDFIFVPENYSFAALIVPQARDLGYKGPFMGSDAWGSAELFNLCGKDCVGQYFSTHYTAE
CCEEEECCCCCEEEEEECCHHCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHEEECC
GATGKTREFIDKYKAKYGYVPDDVAALTWDSINIVLQAIQNAGKVDPDLKKQRKIIRDNM
CCCCCHHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AAIENFDGITGSMKFDDNGDPIKCAVIVKVTDSGSFAFVESVCPK
HHHHCCCCCCEEEEECCCCCCEEEEEEEEEECCCCEEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]