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The map label for this gene is ispH

Identifier: 220905345

GI number: 220905345

Start: 2508158

End: 2509018

Strand: Direct

Name: ispH

Synonym: Ddes_2083

Alternate gene names: 220905345

Gene position: 2508158-2509018 (Clockwise)

Preceding gene: 220905344

Following gene: 220905346

Centisome position: 87.29

GC content: 61.67

Gene sequence:

>861_bases
ATGAACGTACGCCGTGCCAAAACAGCGGGCTTCTGCATGGGCGTGAGCCTCGCCCTGCAAAAACTCAATACCGCCCTGGA
GCGCCGCGCCGGCCAGAGCGCGGGAGCGGGGCGCATATGCACGCTCGGCCCCATTATCCACAACCCGCAGGTACTGGCGG
AGTATGAGACCCGTGGTGTGGTTTGCGTCAATGACCCGGCGCAGCTGCGCCAGGATGATGTGGCCGTCATACGCGCGCAC
GGCATCACCCGGCAGGTGGAACAGGCCGTATGCGCAAGCGGGGCCACCGTGGTGGACGCCACCTGCCCCAAGGTCAAAAA
GGCGCAGCTTTCCATCGCCCGCGCCACATCGCAGGGGGCGACCCTGCTGCTTTTCGGCGAAGAAGATCACCCCGAGGTAC
GCGGCCTCATATCCTACGCCTGCGGCCCGGCCCACGTATTCGGCAATGCCGTAGAACTGGCGGCCCTGCCTCTTGACCCC
GCGCAGCCCTACGTGCTGGCTTCGCAGACCACGCAGGACCGCGAAATATTTACTTCCATTCAGGAAAGCCTGAGCCGCGC
CCTGGACGACCTCACGATTTTATCCACCATTTGCGATGCCACCCGCGAGCGGCAGGAAGAGGCCAGAAACATTGCCTCAT
CGGTAGATGTCATGGTAGTCGTTGGTGGACGACAGAGCGGCAATACCCGGCGACTTGCGGATGTGGCCGCACTGAACGGC
ATCGCCACCTATCATGTGGAGAGCGCCGAAGAGCTTGAACCGAAAAATTTTGCAAATAAACCCCGTGTGGGCCTTACGGC
TGGCGCATCTACGCCGAAAAGCCTGATAGACGCCGCCGAAAAGTGGCTTTCTTCGCTATAA

Upstream 100 bases:

>100_bases
TGCCTGACGGTTTGAGACTCTGGCCGCCTGATACCGGGCAACCGCCCAGATGAGACGCAGGGCCGCGCCTGCAAGCGCGC
ACGCCCTCCAAGGAGAATAT

Downstream 100 bases:

>100_bases
CGTCCCGCACGCGCGACACGATTTTTGGGAGGCAGCATGTCCCAGACCAATATCCTGCTTGAAGCCGGCACCAATGAGCT
GGAAGTGGTAGAATTTTATC

Product: hydroxymethylbutenyl pyrophosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MNVRRAKTAGFCMGVSLALQKLNTALERRAGQSAGAGRICTLGPIIHNPQVLAEYETRGVVCVNDPAQLRQDDVAVIRAH
GITRQVEQAVCASGATVVDATCPKVKKAQLSIARATSQGATLLLFGEEDHPEVRGLISYACGPAHVFGNAVELAALPLDP
AQPYVLASQTTQDREIFTSIQESLSRALDDLTILSTICDATRERQEEARNIASSVDVMVVVGGRQSGNTRRLADVAALNG
IATYHVESAEELEPKNFANKPRVGLTAGASTPKSLIDAAEKWLSSL

Sequences:

>Translated_286_residues
MNVRRAKTAGFCMGVSLALQKLNTALERRAGQSAGAGRICTLGPIIHNPQVLAEYETRGVVCVNDPAQLRQDDVAVIRAH
GITRQVEQAVCASGATVVDATCPKVKKAQLSIARATSQGATLLLFGEEDHPEVRGLISYACGPAHVFGNAVELAALPLDP
AQPYVLASQTTQDREIFTSIQESLSRALDDLTILSTICDATRERQEEARNIASSVDVMVVVGGRQSGNTRRLADVAALNG
IATYHVESAEELEPKNFANKPRVGLTAGASTPKSLIDAAEKWLSSL
>Mature_286_residues
MNVRRAKTAGFCMGVSLALQKLNTALERRAGQSAGAGRICTLGPIIHNPQVLAEYETRGVVCVNDPAQLRQDDVAVIRAH
GITRQVEQAVCASGATVVDATCPKVKKAQLSIARATSQGATLLLFGEEDHPEVRGLISYACGPAHVFGNAVELAALPLDP
AQPYVLASQTTQDREIFTSIQESLSRALDDLTILSTICDATRERQEEARNIASSVDVMVVVGGRQSGNTRRLADVAALNG
IATYHVESAEELEPKNFANKPRVGLTAGASTPKSLIDAAEKWLSSL

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=281, Percent_Identity=27.0462633451957, Blast_Score=114, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_DESDA (B8J3G9)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480657.1
- GeneID:   7285799
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_2083
- HOGENOM:   HBG335228
- ProtClustDB:   CLSK704379
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 30398; Mature: 30398

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVRRAKTAGFCMGVSLALQKLNTALERRAGQSAGAGRICTLGPIIHNPQVLAEYETRGV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHEEEECCCCE
VCVNDPAQLRQDDVAVIRAHGITRQVEQAVCASGATVVDATCPKVKKAQLSIARATSQGA
EEECCHHHHCCCCCEEEECCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCC
TLLLFGEEDHPEVRGLISYACGPAHVFGNAVELAALPLDPAQPYVLASQTTQDREIFTSI
EEEEECCCCCHHHHHHHHHCCCHHHHHCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHH
QESLSRALDDLTILSTICDATRERQEEARNIASSVDVMVVVGGRQSGNTRRLADVAALNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCC
IATYHVESAEELEPKNFANKPRVGLTAGASTPKSLIDAAEKWLSSL
EEEEEECCHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNVRRAKTAGFCMGVSLALQKLNTALERRAGQSAGAGRICTLGPIIHNPQVLAEYETRGV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHEEEECCCCE
VCVNDPAQLRQDDVAVIRAHGITRQVEQAVCASGATVVDATCPKVKKAQLSIARATSQGA
EEECCHHHHCCCCCEEEECCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCC
TLLLFGEEDHPEVRGLISYACGPAHVFGNAVELAALPLDPAQPYVLASQTTQDREIFTSI
EEEEECCCCCHHHHHHHHHCCCHHHHHCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHH
QESLSRALDDLTILSTICDATRERQEEARNIASSVDVMVVVGGRQSGNTRRLADVAALNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCC
IATYHVESAEELEPKNFANKPRVGLTAGASTPKSLIDAAEKWLSSL
EEEEEECCHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA