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The map label for this gene is dus1 [H]

Identifier: 220905344

GI number: 220905344

Start: 2507077

End: 2508072

Strand: Direct

Name: dus1 [H]

Synonym: Ddes_2082

Alternate gene names: 220905344

Gene position: 2507077-2508072 (Clockwise)

Preceding gene: 220905343

Following gene: 220905345

Centisome position: 87.25

GC content: 66.06

Gene sequence:

>996_bases
GTGAGCAAGGCCTTTTCCCTGCCCGTCTGCCCCTGTAACCCGGCAAGCCTGCCCATTGGTCGCGAACATCCCTGGCTGGC
CCCGCTGGCGGGCTACAGCGACCTGCCTTTCAGGCTGCTCTGCCGCCAGTACGGAGCCGCCGTTTGCGTGACGGAAATGG
TCAGCGCCAAGGGGCTGGTGTACCAAAGCCCCGGCACCAACGAGCTTCTGGCGAGCCTGCCCGAAGACCAACCGCTTGTT
GTACAGCTTTTCGGAGCCGAAGCCCCGTTTCTTTCCCGCGCCGTCAGCCTGCTGCGCGATGCGGGCTACGGCTGGTTTGA
CCTCAACATGGGCTGTTCTGTGGCAAAAGTACTGCGTCAGGGCGCGGGAGCGGCCATGCTTGGCGATACAGGCAATATTC
TTGAAGTGGCCCGCGGCATGATCGCAGCCGCCGGCCCCGGCCGCGTGGGTTTCAAGCTGCGCCTCGGCCTTGACGACACG
CGCCCGGTACTGCCTGACCTGGCCCTGCGGCTTGAAGACGCGGGTGCGGGCTGGCTTGTGCTGCATCCGCGCACGGCCCG
TCAGGGTTTTGGCGGCACAGCACAGTGGGAAGCGCTGGCCGACCTTGCCCCGCGCCTGTCCATTCCCCTGCTGGCCAGCG
GCGATCTTTTTTCCGCCGCCGAGGGTATGGCCTGCCTCGAACAGACCGGCGCTGCAGGCCTCATGTATGCGCGCGGGGCC
ATGTACAATCCCGCTATTTTCGCTGATCATGCCGCCCTGTGCGCAGGCGGCCATCCGCAGGCCCATGACGCAGCCTCGCT
CAAGGCCATGATTTTGCGCCACATGGAACTTGCACGCCTGCACTGCCCCGGCAAGGCCGCGGTATGGAAGATGCGCAGCG
TCGTGCCGCGCTACGTGCGCGCCCTGCCCGGCGCACGGGCCTTGCGGCAGGAACTGTGCCGCTGCTCCACCTGGGAAGAA
CTGACCGAAGCGCTGGACAGATGCCTGACGGTTTGA

Upstream 100 bases:

>100_bases
AGCTGCGGCGTACGCCGGCACGGTCCCGAACGCCGGCACGCCCTGCCGCAGCCGGGGCAGGACATCCGCGGGACGACACG
CTTCTCCATAAAGGATCACC

Downstream 100 bases:

>100_bases
GACTCTGGCCGCCTGATACCGGGCAACCGCCCAGATGAGACGCAGGGCCGCGCCTGCAAGCGCGCACGCCCTCCAAGGAG
AATATATGAACGTACGCCGT

Product: dihydrouridine synthase DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSKAFSLPVCPCNPASLPIGREHPWLAPLAGYSDLPFRLLCRQYGAAVCVTEMVSAKGLVYQSPGTNELLASLPEDQPLV
VQLFGAEAPFLSRAVSLLRDAGYGWFDLNMGCSVAKVLRQGAGAAMLGDTGNILEVARGMIAAAGPGRVGFKLRLGLDDT
RPVLPDLALRLEDAGAGWLVLHPRTARQGFGGTAQWEALADLAPRLSIPLLASGDLFSAAEGMACLEQTGAAGLMYARGA
MYNPAIFADHAALCAGGHPQAHDAASLKAMILRHMELARLHCPGKAAVWKMRSVVPRYVRALPGARALRQELCRCSTWEE
LTEALDRCLTV

Sequences:

>Translated_331_residues
MSKAFSLPVCPCNPASLPIGREHPWLAPLAGYSDLPFRLLCRQYGAAVCVTEMVSAKGLVYQSPGTNELLASLPEDQPLV
VQLFGAEAPFLSRAVSLLRDAGYGWFDLNMGCSVAKVLRQGAGAAMLGDTGNILEVARGMIAAAGPGRVGFKLRLGLDDT
RPVLPDLALRLEDAGAGWLVLHPRTARQGFGGTAQWEALADLAPRLSIPLLASGDLFSAAEGMACLEQTGAAGLMYARGA
MYNPAIFADHAALCAGGHPQAHDAASLKAMILRHMELARLHCPGKAAVWKMRSVVPRYVRALPGARALRQELCRCSTWEE
LTEALDRCLTV
>Mature_330_residues
SKAFSLPVCPCNPASLPIGREHPWLAPLAGYSDLPFRLLCRQYGAAVCVTEMVSAKGLVYQSPGTNELLASLPEDQPLVV
QLFGAEAPFLSRAVSLLRDAGYGWFDLNMGCSVAKVLRQGAGAAMLGDTGNILEVARGMIAAAGPGRVGFKLRLGLDDTR
PVLPDLALRLEDAGAGWLVLHPRTARQGFGGTAQWEALADLAPRLSIPLLASGDLFSAAEGMACLEQTGAAGLMYARGAM
YNPAIFADHAALCAGGHPQAHDAASLKAMILRHMELARLHCPGKAAVWKMRSVVPRYVRALPGARALRQELCRCSTWEEL
TEALDRCLTV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=30.6666666666667, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI239788462, Length=241, Percent_Identity=29.4605809128631, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI239788483, Length=240, Percent_Identity=29.1666666666667, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI40807366, Length=226, Percent_Identity=32.3008849557522, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI8923374, Length=243, Percent_Identity=28.8065843621399, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1789660, Length=223, Percent_Identity=31.390134529148, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788462, Length=231, Percent_Identity=29.8701298701299, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=225, Percent_Identity=33.7777777777778, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17507177, Length=231, Percent_Identity=31.1688311688312, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=233, Percent_Identity=27.0386266094421, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=246, Percent_Identity=26.8292682926829, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323437, Length=239, Percent_Identity=29.2887029288703, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323560, Length=319, Percent_Identity=26.9592476489028, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=259, Percent_Identity=24.3243243243243, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24580595, Length=224, Percent_Identity=31.6964285714286, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI19920448, Length=224, Percent_Identity=31.6964285714286, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI19921524, Length=224, Percent_Identity=32.5892857142857, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24585320, Length=236, Percent_Identity=30.0847457627119, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35242; Mature: 35111

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAFSLPVCPCNPASLPIGREHPWLAPLAGYSDLPFRLLCRQYGAAVCVTEMVSAKGLV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCEE
YQSPGTNELLASLPEDQPLVVQLFGAEAPFLSRAVSLLRDAGYGWFDLNMGCSVAKVLRQ
EECCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHC
GAGAAMLGDTGNILEVARGMIAAAGPGRVGFKLRLGLDDTRPVLPDLALRLEDAGAGWLV
CCCEEEECCCCCHHHHHHHHHEECCCCCCCEEEEECCCCCCCHHHHHHHEEECCCCCEEE
LHPRTARQGFGGTAQWEALADLAPRLSIPLLASGDLFSAAEGMACLEQTGAAGLMYARGA
ECCCHHHCCCCCCHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCHHHHHCCC
MYNPAIFADHAALCAGGHPQAHDAASLKAMILRHMELARLHCPGKAAVWKMRSVVPRYVR
CCCCCHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ALPGARALRQELCRCSTWEELTEALDRCLTV
HCCCHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKAFSLPVCPCNPASLPIGREHPWLAPLAGYSDLPFRLLCRQYGAAVCVTEMVSAKGLV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCEE
YQSPGTNELLASLPEDQPLVVQLFGAEAPFLSRAVSLLRDAGYGWFDLNMGCSVAKVLRQ
EECCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHC
GAGAAMLGDTGNILEVARGMIAAAGPGRVGFKLRLGLDDTRPVLPDLALRLEDAGAGWLV
CCCEEEECCCCCHHHHHHHHHEECCCCCCCEEEEECCCCCCCHHHHHHHEEECCCCCEEE
LHPRTARQGFGGTAQWEALADLAPRLSIPLLASGDLFSAAEGMACLEQTGAAGLMYARGA
ECCCHHHCCCCCCHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCHHHHHCCC
MYNPAIFADHAALCAGGHPQAHDAASLKAMILRHMELARLHCPGKAAVWKMRSVVPRYVR
CCCCCHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ALPGARALRQELCRCSTWEELTEALDRCLTV
HCCCHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]