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The map label for this gene is lipA [H]

Identifier: 220905338

GI number: 220905338

Start: 2498481

End: 2499380

Strand: Direct

Name: lipA [H]

Synonym: Ddes_2076

Alternate gene names: 220905338

Gene position: 2498481-2499380 (Clockwise)

Preceding gene: 220905337

Following gene: 220905342

Centisome position: 86.95

GC content: 63.44

Gene sequence:

>900_bases
GTGACCTGCAAAACCTCCACATCCGGCTCCTGCGGCGACAGGCCGCAAAAAGCCATGCGCATGCCGCCCTGGCTGCGCCG
ACCACTGGCCTCGGACAAACGCTTTTTTTCCACCACCGGCCTTGTGGATGACCTGGGCCTTTTCACCGTCTGCAAGGCGG
CCAACTGCCCCAACCGGCAGGAATGCTACTCCGCCGGAACAGCTACCTTTCTGATTCTGGGCGAAACCTGCACGCGAAAC
TGCCGCTTCTGTAACATCCACCCCGGTCAGACCGCTGCCCCCAGCCCCGATGAGCCGGAGCGCGTGGCCAAGGCCGCACG
CACCCTGGGCCTACGGCATATTGTCATCACCTCGGTCACGCGGGACGATCTGGAAGACGGCGGAGCCGGGCATTTTGCCC
GCACCATCACCGCCGTGCGCATGGCGCTGCCCCAGAGCAGCATCGAGGTACTCATTCCCGATTTTCAGGGCAACGAACAG
GCCCTGCGTACGGTTATGGACGCCGCCCCCCATGTGATCAACCACAATGTGGAAACCCATCCGGCGCTGTACAGCAGGGT
GCGCCCGCAGGCCCGCTACGAGCAGAGCCTGGAACTGCTGCGCAGGGTACATGCTGCCGGGGGTACGGCCAAAAGCGGCC
TTATGGTGGGCCTTGGCGAAACTGATGACCAGGTGCGCGAAGTCATCCGCGACCTGCACGCCGCAGGCTGCTCCATCATC
ACCATCGGCCAGTACCTGCCCCCCAGCAAGGAGCACCACCCCCTTGACCGCTATGTAACGCCGGAACAGTTTGAAGCCTA
TGCCGACTACGGAAAATCACTGGGCGTCAGGCACGTCTTTTCTGCCCCGCTGGTGCGCAGCAGCTACCAGGCGGGAAGTT
TCGTGCAGCCCGGGGCGTAA

Upstream 100 bases:

>100_bases
ATATTTTCCAGCCCGCCGGGGACCATGCCCAGCCTCTGCCCACCCTGCGGACCACCCAAGACCTCATGACACTGTTGCAA
GACGCGCAAAGGACTGAAAA

Downstream 100 bases:

>100_bases
CACGCCGCGCATTGAAAACACGCACTCCCGCGCCGGAGCAGATTTTTTGCTGAAATAATGCCGCAACTCACCTGCGGGTA
AAGTGTATCCACAAGTAAAG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTCKTSTSGSCGDRPQKAMRMPPWLRRPLASDKRFFSTTGLVDDLGLFTVCKAANCPNRQECYSAGTATFLILGETCTRN
CRFCNIHPGQTAAPSPDEPERVAKAARTLGLRHIVITSVTRDDLEDGGAGHFARTITAVRMALPQSSIEVLIPDFQGNEQ
ALRTVMDAAPHVINHNVETHPALYSRVRPQARYEQSLELLRRVHAAGGTAKSGLMVGLGETDDQVREVIRDLHAAGCSII
TIGQYLPPSKEHHPLDRYVTPEQFEAYADYGKSLGVRHVFSAPLVRSSYQAGSFVQPGA

Sequences:

>Translated_299_residues
MTCKTSTSGSCGDRPQKAMRMPPWLRRPLASDKRFFSTTGLVDDLGLFTVCKAANCPNRQECYSAGTATFLILGETCTRN
CRFCNIHPGQTAAPSPDEPERVAKAARTLGLRHIVITSVTRDDLEDGGAGHFARTITAVRMALPQSSIEVLIPDFQGNEQ
ALRTVMDAAPHVINHNVETHPALYSRVRPQARYEQSLELLRRVHAAGGTAKSGLMVGLGETDDQVREVIRDLHAAGCSII
TIGQYLPPSKEHHPLDRYVTPEQFEAYADYGKSLGVRHVFSAPLVRSSYQAGSFVQPGA
>Mature_298_residues
TCKTSTSGSCGDRPQKAMRMPPWLRRPLASDKRFFSTTGLVDDLGLFTVCKAANCPNRQECYSAGTATFLILGETCTRNC
RFCNIHPGQTAAPSPDEPERVAKAARTLGLRHIVITSVTRDDLEDGGAGHFARTITAVRMALPQSSIEVLIPDFQGNEQA
LRTVMDAAPHVINHNVETHPALYSRVRPQARYEQSLELLRRVHAAGGTAKSGLMVGLGETDDQVREVIRDLHAAGCSIIT
IGQYLPPSKEHHPLDRYVTPEQFEAYADYGKSLGVRHVFSAPLVRSSYQAGSFVQPGA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=40, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI37577164, Length=243, Percent_Identity=39.0946502057613, Blast_Score=188, Evalue=4e-48,
Organism=Escherichia coli, GI1786846, Length=277, Percent_Identity=46.2093862815885, Blast_Score=254, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI32564533, Length=295, Percent_Identity=40.3389830508475, Blast_Score=226, Evalue=9e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=291, Percent_Identity=39.5189003436426, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI221513272, Length=288, Percent_Identity=38.8888888888889, Blast_Score=225, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 32672; Mature: 32541

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCKTSTSGSCGDRPQKAMRMPPWLRRPLASDKRFFSTTGLVDDLGLFTVCKAANCPNRQ
CCCCCCCCCCCCCCCHHHHCCCHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCHH
ECYSAGTATFLILGETCTRNCRFCNIHPGQTAAPSPDEPERVAKAARTLGLRHIVITSVT
HHHHCCCEEEEEECCHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCC
RDDLEDGGAGHFARTITAVRMALPQSSIEVLIPDFQGNEQALRTVMDAAPHVINHNVETH
HHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCHHHCCCCCCC
PALYSRVRPQARYEQSLELLRRVHAAGGTAKSGLMVGLGETDDQVREVIRDLHAAGCSII
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEE
TIGQYLPPSKEHHPLDRYVTPEQFEAYADYGKSLGVRHVFSAPLVRSSYQAGSFVQPGA
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TCKTSTSGSCGDRPQKAMRMPPWLRRPLASDKRFFSTTGLVDDLGLFTVCKAANCPNRQ
CCCCCCCCCCCCCCHHHHCCCHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCHH
ECYSAGTATFLILGETCTRNCRFCNIHPGQTAAPSPDEPERVAKAARTLGLRHIVITSVT
HHHHCCCEEEEEECCHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCC
RDDLEDGGAGHFARTITAVRMALPQSSIEVLIPDFQGNEQALRTVMDAAPHVINHNVETH
HHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCHHHCCCCCCC
PALYSRVRPQARYEQSLELLRRVHAAGGTAKSGLMVGLGETDDQVREVIRDLHAAGCSII
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEE
TIGQYLPPSKEHHPLDRYVTPEQFEAYADYGKSLGVRHVFSAPLVRSSYQAGSFVQPGA
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA