Click here to switch to the map view.

The map label for this gene is hybA [C]

Identifier: 220905301

GI number: 220905301

Start: 2451824

End: 2452927

Strand: Direct

Name: hybA [C]

Synonym: Ddes_2039

Alternate gene names: 220905301

Gene position: 2451824-2452927 (Clockwise)

Preceding gene: 220905300

Following gene: 220905302

Centisome position: 85.33

GC content: 62.68

Gene sequence:

>1104_bases
ATGAAACGCAGAACATTTCTGACCATTCTGGGAAGCGCGGGCGTGGTCTCGGCCCTTGGCTCAGCCAAGGTGGCCCAGGC
CGGCCCCCATACCTTCCCATATTACGCAGACAGTTACGGGGTGCTGCACGACACCACCCGTTGCATCGGTTGCCGCAAGT
GTGAAGAGGCCTGCAACAAGGTCAACCACCTGCCCAAACCCAAAAAACCCTTCAGCGACCTTACGGTGTGCGACACGCAC
CGCCGCACGGGCGAATATGAATGGACCGTGGTGAACAAGTACAAGGTCAACGGCAAGGACGTATTCCGCAAGCTGCAGTG
CTTTCACTGCAACGACCCGGCCTGCGCTTCCGCCTGCTTTGCCAAGTGCTTTACCAAGCAGCCCAACGGCGCTGTTCACT
ACGAAGGCTCACAGTGCGTGGGCTGCCGTTACTGCATGGTCGCCTGCCCCTTCTATGTTCCCGGCTTCCAGTACGATGAG
GCCTTTGACCCCCTCGTGCAAAAGTGCACCTTCTGCGAGCCGCTGCTCAAGGAAGGCAAGCTGCCCGGCTGTGTGCAGGC
CTGCCCCAAGGACGCCCTCACCTTCGGCCGCCGCAGCGACCTGATCCGAGTGGCCCGCGCCCGCATTGCCGATAATCCCG
GCAAGTACGTCAACTACCTCTATGGCGAGTTTGACGCAGGCGGCACTGCCTGGATGGTGCTCAGCCCCGCAGTGGGACAG
GCAGCCGCGGCTGTTCCGGCGGATGATGCTCCGCTGGCTGGCGATGAACTGAAGCAGCTCGGCCTTGATACCCATCTCGG
CAACCGCCCCATGGGCGAGCTGACCTACGGCGCTCTGGGCGCAGTGCCTATGATCGTGGCCTTCTGGCCCGTGCTCTTCG
GCGGTGCTTACGCCATCAGCAAACGTCGCGAAGCCATGTACAAGGCTGAAACGGATGGCAAGGTCAAGGAGGCCCGTGAA
GACGTGGCCGCCGCCGTTGACGCCGCCGTACGCAAAATTGAGGAAACCCAGGGGCCGGGCGCTGCCGATACGGCCCGCCG
CGCCATGACAGAGGCCCTCAAGGCCCGCGAAACGGCGTGCACGGGGCACGGGGAGGATAAGTAA

Upstream 100 bases:

>100_bases
GGTTGCCACAAGGGCATGGATGTCGCGCGTCCTCGTGACACCGACTGCACCACTTGCCATAAGGCTGCGCCCAAGAGCGC
CGACTAGCATGGAGTAAGAT

Downstream 100 bases:

>100_bases
ATGTCACAGCACAGCATCGTCATTCCCAGCAAGGGCAAGCTGTTTAACCTGGACGAGTTTCTCAAACCTACTCCGGGCAA
TATCATCACCGCCATCATCC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: NA

Alternate protein names: HMC operon ORF 2 [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKRRTFLTILGSAGVVSALGSAKVAQAGPHTFPYYADSYGVLHDTTRCIGCRKCEEACNKVNHLPKPKKPFSDLTVCDTH
RRTGEYEWTVVNKYKVNGKDVFRKLQCFHCNDPACASACFAKCFTKQPNGAVHYEGSQCVGCRYCMVACPFYVPGFQYDE
AFDPLVQKCTFCEPLLKEGKLPGCVQACPKDALTFGRRSDLIRVARARIADNPGKYVNYLYGEFDAGGTAWMVLSPAVGQ
AAAAVPADDAPLAGDELKQLGLDTHLGNRPMGELTYGALGAVPMIVAFWPVLFGGAYAISKRREAMYKAETDGKVKEARE
DVAAAVDAAVRKIEETQGPGAADTARRAMTEALKARETACTGHGEDK

Sequences:

>Translated_367_residues
MKRRTFLTILGSAGVVSALGSAKVAQAGPHTFPYYADSYGVLHDTTRCIGCRKCEEACNKVNHLPKPKKPFSDLTVCDTH
RRTGEYEWTVVNKYKVNGKDVFRKLQCFHCNDPACASACFAKCFTKQPNGAVHYEGSQCVGCRYCMVACPFYVPGFQYDE
AFDPLVQKCTFCEPLLKEGKLPGCVQACPKDALTFGRRSDLIRVARARIADNPGKYVNYLYGEFDAGGTAWMVLSPAVGQ
AAAAVPADDAPLAGDELKQLGLDTHLGNRPMGELTYGALGAVPMIVAFWPVLFGGAYAISKRREAMYKAETDGKVKEARE
DVAAAVDAAVRKIEETQGPGAADTARRAMTEALKARETACTGHGEDK
>Mature_367_residues
MKRRTFLTILGSAGVVSALGSAKVAQAGPHTFPYYADSYGVLHDTTRCIGCRKCEEACNKVNHLPKPKKPFSDLTVCDTH
RRTGEYEWTVVNKYKVNGKDVFRKLQCFHCNDPACASACFAKCFTKQPNGAVHYEGSQCVGCRYCMVACPFYVPGFQYDE
AFDPLVQKCTFCEPLLKEGKLPGCVQACPKDALTFGRRSDLIRVARARIADNPGKYVNYLYGEFDAGGTAWMVLSPAVGQ
AAAAVPADDAPLAGDELKQLGLDTHLGNRPMGELTYGALGAVPMIVAFWPVLFGGAYAISKRREAMYKAETDGKVKEARE
DVAAAVDAAVRKIEETQGPGAADTARRAMTEALKARETACTGHGEDK

Specific function: HMWC (high-molecular-weight cytochrome c precursor), ORF2, ORF3, ORF4, ORF5, ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=267, Percent_Identity=34.4569288389513, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1790326, Length=212, Percent_Identity=32.5471698113208, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787749, Length=221, Percent_Identity=30.7692307692308, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI2367345, Length=200, Percent_Identity=36.5, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI226510944, Length=198, Percent_Identity=34.3434343434343, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787122, Length=168, Percent_Identity=36.9047619047619, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787872, Length=168, Percent_Identity=35.1190476190476, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082180, Length=168, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI2367245, Length=177, Percent_Identity=28.8135593220339, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789079, Length=199, Percent_Identity=27.1356783919598, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 39810; Mature: 39810

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
5.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRTFLTILGSAGVVSALGSAKVAQAGPHTFPYYADSYGVLHDTTRCIGCRKCEEACNK
CCCCEEEEEECCCHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VNHLPKPKKPFSDLTVCDTHRRTGEYEWTVVNKYKVNGKDVFRKLQCFHCNDPACASACF
HHCCCCCCCCCCCCEEECCCCCCCCEEEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHH
AKCFTKQPNGAVHYEGSQCVGCRYCMVACPFYVPGFQYDEAFDPLVQKCTFCEPLLKEGK
HHHHCCCCCCCEEECCCCCCHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LPGCVQACPKDALTFGRRSDLIRVARARIADNPGKYVNYLYGEFDAGGTAWMVLSPAVGQ
CCHHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHEEECCCCEEEEEECCCCCC
AAAAVPADDAPLAGDELKQLGLDTHLGNRPMGELTYGALGAVPMIVAFWPVLFGGAYAIS
HHHCCCCCCCCCCHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
KRREAMYKAETDGKVKEAREDVAAAVDAAVRKIEETQGPGAADTARRAMTEALKARETAC
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
TGHGEDK
CCCCCCC
>Mature Secondary Structure
MKRRTFLTILGSAGVVSALGSAKVAQAGPHTFPYYADSYGVLHDTTRCIGCRKCEEACNK
CCCCEEEEEECCCHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VNHLPKPKKPFSDLTVCDTHRRTGEYEWTVVNKYKVNGKDVFRKLQCFHCNDPACASACF
HHCCCCCCCCCCCCEEECCCCCCCCEEEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHH
AKCFTKQPNGAVHYEGSQCVGCRYCMVACPFYVPGFQYDEAFDPLVQKCTFCEPLLKEGK
HHHHCCCCCCCEEECCCCCCHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LPGCVQACPKDALTFGRRSDLIRVARARIADNPGKYVNYLYGEFDAGGTAWMVLSPAVGQ
CCHHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHEEECCCCEEEEEECCCCCC
AAAAVPADDAPLAGDELKQLGLDTHLGNRPMGELTYGALGAVPMIVAFWPVLFGGAYAIS
HHHCCCCCCCCCCHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
KRREAMYKAETDGKVKEAREDVAAAVDAAVRKIEETQGPGAADTARRAMTEALKARETAC
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
TGHGEDK
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]