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The map label for this gene is mrcA [H]

Identifier: 220905180

GI number: 220905180

Start: 2310197

End: 2312635

Strand: Direct

Name: mrcA [H]

Synonym: Ddes_1918

Alternate gene names: 220905180

Gene position: 2310197-2312635 (Clockwise)

Preceding gene: 220905179

Following gene: 220905181

Centisome position: 80.4

GC content: 59.78

Gene sequence:

>2439_bases
ATGAAAATTCGTCTGTCATGGAAAAAACTCACCCTTTGGCTGGTGAGTATACTGCTGGTCTGCGGCCTTCTTGGCGGCGG
CGCTGTGGTCATGCTGTTTTACTGGGCCTCACGCGACCTGCCCGACATCAACCGCATTGCAGAATACAAGCAGCCTCAGG
CTACTGTCATTCTGGCACGGGACGGCTCCACACTGGGTACGCTGTACCATGAAAAACGCTTTGTTATCGGTCTCAAGGAA
ATGTCGCGCTTTCTGCCCATGGCCTTTCTGGCTGCAGAAGACGATGCTTTCTACCGGCACATGGGCGTAGACCCTACAGC
CATTCTGCGCGCGGCCATCAACAACTTTCGCAAGGGGCGGCAGGGCGAGGGCGGCAGCACCATCACCCAGCAGCTTATCA
AGCAGCTTTTGCTGACCTCCGAGCGCAGCTATACCCGCAAGATGAAAGAGGCCATTCTGGCCTATGATCTGGAAAAAACC
TACACCAAGGACCAGATACTCACCATCTATCTGAACCAGATATATCTGGGCGAGCACGCCTATGGCGTGGAGGCGGCCGC
GCGCACCTATTTCGGCAAGCACGCCTCGGACATCACCCTGGCTGAAAGCGCCGTTATTGCCGGCCTGCCCAAGGCCCCCA
GCACTTACAATCCCTTCCGCCGCCCGGAAGAGGCCAAAAATCGCCAGATGTACGTTCTTGGCCGCCTGCGCGACCTCAAA
TGGATCAGCCCCGAAGAATACGAAAAGGCCGCCGCCGAACCTCTGGTATACTGGAGCATGCCCGAAGGCATGGGGGGCGC
GGCCCTGTGGTATCTTGAAGAAACCCGCCGCCTGCTCATAGAATTTTTTACGGAACAGAACCTTAAGGCGCTGGGGGTTG
AAACGGACAAGTTCGGCGTGGACTATGTGTATGAAGCGGGCCTTACCGTGCGTACCGCTATGGACCCACCCCATCAGTAC
GCCGCCGGGGCAGCCCTGCGGCAGGGCCTGGAAGAGCTGGACAAGCGCCAGGGCTGGCGCGGCCCGCTGGAAAAACTGGA
TGCTGAACAGCAAAAAGCCTTTCCGGCACAAAAAGCCTTCAGTCCCCTGGACCTTGCAGGCAACAACTGGGTCAAGGCCC
TTGTTGTTGCAGCAGACGCCAAAGAAGCCCGCGTAAACCTGGGGCAGGGCTATACCGGCCTTATCCCTGTGGGCAACATG
TCGTGGGCGCGCAAGCCCAACCCCAGGGTGGCGGGCATCTACGCTCCGGCTGTCAAGGACGCCCGGCTTGTGGTCAGCCC
CGGCGACCTGATCTGGGTTTCCGCCACGGAAACCACGGTTACCGAAACCAATGCCAAGGGCCGCAAGGAAAAGAAAACTA
TCCCCTTCGACCCGTCGACTGCCAAAAAAGGCACTCCCATCGCCTTGCTGCTGCAACAGGAACCGGCTGTGCAGGGGGCC
ATTGCCTCGGTAGAGGCCGAAAATGGCGATGTGGTGGCCCTTATTGGCGGCTACCAGTTTGGCGACAGCCATTTCAACCG
TGCCACCCAGGCCCGCCGCCAGCCCGGCTCCAGCTTCAAACCTGTGGTCTACTCCACGGCCCTGGATATGGGCTTTACCG
CCGCCTCCACCGTGCTTGATGCACCCTTCGTCTATGTGAACCCCTATACCAACGAGGTATGGCGGCCCTCAAACTATGAA
AAGAACTACAAGGGCGAACTGCCCCTGCACCAGGCCCTGACGCTCTCGCGCAATACCTGCACTGTGCGCATCGCCCAGCA
GGTAGGCATTGCCAATGTCATCCAGCGGGCCAAGGCGCTGGGGCTTGAACCCCACTTTCCGCAGGAACTTTCAGTCAGCC
TCGGCGCTGTGGCCGTTTCCCCCCTCAACCTGACCCAGGCTTACGCCGCCTTTGCCAACAAGGGGCTTGGCGTGCGCCCG
CGCATCATCACCTCCATAAGCGATCCGCAGGGCCGTGAGCTTTATCGCCAGAACGTGGAACAATGGCAGGCCGTCACTCC
GCAGAACGCCTATATCATGGCAACCCTGCTCAAGCAGGTGGTCAATGCGGGTACGGGCAGTCGCGCCAGGGTGCAGGGAT
TCAACATCGGCGGCAAAACCGGCACCACTAACGAAGAACACGATGCCTGGTTTGTGGGCTTTTCGCCCCACCTTGTCACG
GGCGTCTATGTGGGCTACGACCAGTTGCAGTCTCTGGGCCGTCTGGAACAGGGCGGGCGCACCGCCGCTCCCATCTTCCG
CTATTACCGTGTGGAGGTGGAAGACCGCTACCCCGCAGACGATTTTGCCATGCCCGAGGGCATCACCATGATGGACGGGC
TTGCTTTCCGCACCGACATGCCCATACAGGGCGTTTCGGCCACAGCTGCCACCACCGAGGATGACAGCGTGGTGGACACC
TCGCAGGGCGGCGAAGACCTCATGCGCCAGATGTTTTAA

Upstream 100 bases:

>100_bases
CCGGCAAAAAGCTTTTCTGGCGCGCCATCCGCAACAACCTGCCCGGCGCGGGATCGCGCGCATACTTTACGCCGGGGCAA
AAAAACGGTATCGCCCTGCC

Downstream 100 bases:

>100_bases
AGCATTTTCAAAGTGAAAATGCCTGGCGGCTGCACGGGCAGACGCCCGCCGTGGAGGCGCAAGTGCAATGTATTTACGCT
GTCAAACGTCGGCGGGCGCG

Product: penicillin-binding protein, 1A family

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 812; Mature: 812

Protein sequence:

>812_residues
MKIRLSWKKLTLWLVSILLVCGLLGGGAVVMLFYWASRDLPDINRIAEYKQPQATVILARDGSTLGTLYHEKRFVIGLKE
MSRFLPMAFLAAEDDAFYRHMGVDPTAILRAAINNFRKGRQGEGGSTITQQLIKQLLLTSERSYTRKMKEAILAYDLEKT
YTKDQILTIYLNQIYLGEHAYGVEAAARTYFGKHASDITLAESAVIAGLPKAPSTYNPFRRPEEAKNRQMYVLGRLRDLK
WISPEEYEKAAAEPLVYWSMPEGMGGAALWYLEETRRLLIEFFTEQNLKALGVETDKFGVDYVYEAGLTVRTAMDPPHQY
AAGAALRQGLEELDKRQGWRGPLEKLDAEQQKAFPAQKAFSPLDLAGNNWVKALVVAADAKEARVNLGQGYTGLIPVGNM
SWARKPNPRVAGIYAPAVKDARLVVSPGDLIWVSATETTVTETNAKGRKEKKTIPFDPSTAKKGTPIALLLQQEPAVQGA
IASVEAENGDVVALIGGYQFGDSHFNRATQARRQPGSSFKPVVYSTALDMGFTAASTVLDAPFVYVNPYTNEVWRPSNYE
KNYKGELPLHQALTLSRNTCTVRIAQQVGIANVIQRAKALGLEPHFPQELSVSLGAVAVSPLNLTQAYAAFANKGLGVRP
RIITSISDPQGRELYRQNVEQWQAVTPQNAYIMATLLKQVVNAGTGSRARVQGFNIGGKTGTTNEEHDAWFVGFSPHLVT
GVYVGYDQLQSLGRLEQGGRTAAPIFRYYRVEVEDRYPADDFAMPEGITMMDGLAFRTDMPIQGVSATAATTEDDSVVDT
SQGGEDLMRQMF

Sequences:

>Translated_812_residues
MKIRLSWKKLTLWLVSILLVCGLLGGGAVVMLFYWASRDLPDINRIAEYKQPQATVILARDGSTLGTLYHEKRFVIGLKE
MSRFLPMAFLAAEDDAFYRHMGVDPTAILRAAINNFRKGRQGEGGSTITQQLIKQLLLTSERSYTRKMKEAILAYDLEKT
YTKDQILTIYLNQIYLGEHAYGVEAAARTYFGKHASDITLAESAVIAGLPKAPSTYNPFRRPEEAKNRQMYVLGRLRDLK
WISPEEYEKAAAEPLVYWSMPEGMGGAALWYLEETRRLLIEFFTEQNLKALGVETDKFGVDYVYEAGLTVRTAMDPPHQY
AAGAALRQGLEELDKRQGWRGPLEKLDAEQQKAFPAQKAFSPLDLAGNNWVKALVVAADAKEARVNLGQGYTGLIPVGNM
SWARKPNPRVAGIYAPAVKDARLVVSPGDLIWVSATETTVTETNAKGRKEKKTIPFDPSTAKKGTPIALLLQQEPAVQGA
IASVEAENGDVVALIGGYQFGDSHFNRATQARRQPGSSFKPVVYSTALDMGFTAASTVLDAPFVYVNPYTNEVWRPSNYE
KNYKGELPLHQALTLSRNTCTVRIAQQVGIANVIQRAKALGLEPHFPQELSVSLGAVAVSPLNLTQAYAAFANKGLGVRP
RIITSISDPQGRELYRQNVEQWQAVTPQNAYIMATLLKQVVNAGTGSRARVQGFNIGGKTGTTNEEHDAWFVGFSPHLVT
GVYVGYDQLQSLGRLEQGGRTAAPIFRYYRVEVEDRYPADDFAMPEGITMMDGLAFRTDMPIQGVSATAATTEDDSVVDT
SQGGEDLMRQMF
>Mature_812_residues
MKIRLSWKKLTLWLVSILLVCGLLGGGAVVMLFYWASRDLPDINRIAEYKQPQATVILARDGSTLGTLYHEKRFVIGLKE
MSRFLPMAFLAAEDDAFYRHMGVDPTAILRAAINNFRKGRQGEGGSTITQQLIKQLLLTSERSYTRKMKEAILAYDLEKT
YTKDQILTIYLNQIYLGEHAYGVEAAARTYFGKHASDITLAESAVIAGLPKAPSTYNPFRRPEEAKNRQMYVLGRLRDLK
WISPEEYEKAAAEPLVYWSMPEGMGGAALWYLEETRRLLIEFFTEQNLKALGVETDKFGVDYVYEAGLTVRTAMDPPHQY
AAGAALRQGLEELDKRQGWRGPLEKLDAEQQKAFPAQKAFSPLDLAGNNWVKALVVAADAKEARVNLGQGYTGLIPVGNM
SWARKPNPRVAGIYAPAVKDARLVVSPGDLIWVSATETTVTETNAKGRKEKKTIPFDPSTAKKGTPIALLLQQEPAVQGA
IASVEAENGDVVALIGGYQFGDSHFNRATQARRQPGSSFKPVVYSTALDMGFTAASTVLDAPFVYVNPYTNEVWRPSNYE
KNYKGELPLHQALTLSRNTCTVRIAQQVGIANVIQRAKALGLEPHFPQELSVSLGAVAVSPLNLTQAYAAFANKGLGVRP
RIITSISDPQGRELYRQNVEQWQAVTPQNAYIMATLLKQVVNAGTGSRARVQGFNIGGKTGTTNEEHDAWFVGFSPHLVT
GVYVGYDQLQSLGRLEQGGRTAAPIFRYYRVEVEDRYPADDFAMPEGITMMDGLAFRTDMPIQGVSATAATTEDDSVVDT
SQGGEDLMRQMF

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=658, Percent_Identity=32.0668693009119, Blast_Score=311, Evalue=1e-85,
Organism=Escherichia coli, GI1786343, Length=189, Percent_Identity=38.6243386243386, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1788867, Length=214, Percent_Identity=32.7102803738318, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789601, Length=193, Percent_Identity=34.1968911917098, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 89685; Mature: 89685

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRLSWKKLTLWLVSILLVCGLLGGGAVVMLFYWASRDLPDINRIAEYKQPQATVILAR
CEEEEEHHHHHHHHHHHHHHHHHCCCCHHEEEEHHHCCCCCCHHHHHHCCCCCEEEEEEE
DGSTLGTLYHEKRFVIGLKEMSRFLPMAFLAAEDDAFYRHMGVDPTAILRAAINNFRKGR
CCCCHHHHHHCCEEEEEHHHHHHHCCCCEEECCCCHHHHHCCCCHHHHHHHHHHHHHCCC
QGEGGSTITQQLIKQLLLTSERSYTRKMKEAILAYDLEKTYTKDQILTIYLNQIYLGEHA
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCC
YGVEAAARTYFGKHASDITLAESAVIAGLPKAPSTYNPFRRPEEAKNRQMYVLGRLRDLK
CCHHHHHHHHHCCCCCCCEEHHHHHHCCCCCCCCCCCCCCCCHHHCCCEEEEEEECCCCC
WISPEEYEKAAAEPLVYWSMPEGMGGAALWYLEETRRLLIEFFTEQNLKALGVETDKFGV
CCCHHHHHHHHCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
DYVYEAGLTVRTAMDPPHQYAAGAALRQGLEELDKRQGWRGPLEKLDAEQQKAFPAQKAF
CEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHCCCHHHCC
SPLDLAGNNWVKALVVAADAKEARVNLGQGYTGLIPVGNMSWARKPNPRVAGIYAPAVKD
CCCCCCCCCCEEEEEEEECCHHHHEECCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCC
ARLVVSPGDLIWVSATETTVTETNAKGRKEKKTIPFDPSTAKKGTPIALLLQQEPAVQGA
CEEEECCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHH
IASVEAENGDVVALIGGYQFGDSHFNRATQARRQPGSSFKPVVYSTALDMGFTAASTVLD
EEEEECCCCCEEEEECCEECCCHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCHHHHHHHC
APFVYVNPYTNEVWRPSNYEKNYKGELPLHQALTLSRNTCTVRIAQQVGIANVIQRAKAL
CCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCEEEEEHHHCCHHHHHHHHHHC
GLEPHFPQELSVSLGAVAVSPLNLTQAYAAFANKGLGVRPRIITSISDPQGRELYRQNVE
CCCCCCCHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHH
QWQAVTPQNAYIMATLLKQVVNAGTGSRARVQGFNIGGKTGTTNEEHDAWFVGFSPHLVT
HHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCHHHH
GVYVGYDQLQSLGRLEQGGRTAAPIFRYYRVEVEDRYPADDFAMPEGITMMDGLAFRTDM
EEEECHHHHHHHHHHHHCCCHHHCEEEEEEEEECCCCCCCCCCCCCCCCHHCCEEEECCC
PIQGVSATAATTEDDSVVDTSQGGEDLMRQMF
CCCCCCCCCCCCCCCCCEECCCCHHHHHHHCC
>Mature Secondary Structure
MKIRLSWKKLTLWLVSILLVCGLLGGGAVVMLFYWASRDLPDINRIAEYKQPQATVILAR
CEEEEEHHHHHHHHHHHHHHHHHCCCCHHEEEEHHHCCCCCCHHHHHHCCCCCEEEEEEE
DGSTLGTLYHEKRFVIGLKEMSRFLPMAFLAAEDDAFYRHMGVDPTAILRAAINNFRKGR
CCCCHHHHHHCCEEEEEHHHHHHHCCCCEEECCCCHHHHHCCCCHHHHHHHHHHHHHCCC
QGEGGSTITQQLIKQLLLTSERSYTRKMKEAILAYDLEKTYTKDQILTIYLNQIYLGEHA
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCC
YGVEAAARTYFGKHASDITLAESAVIAGLPKAPSTYNPFRRPEEAKNRQMYVLGRLRDLK
CCHHHHHHHHHCCCCCCCEEHHHHHHCCCCCCCCCCCCCCCCHHHCCCEEEEEEECCCCC
WISPEEYEKAAAEPLVYWSMPEGMGGAALWYLEETRRLLIEFFTEQNLKALGVETDKFGV
CCCHHHHHHHHCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
DYVYEAGLTVRTAMDPPHQYAAGAALRQGLEELDKRQGWRGPLEKLDAEQQKAFPAQKAF
CEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHCCCHHHCC
SPLDLAGNNWVKALVVAADAKEARVNLGQGYTGLIPVGNMSWARKPNPRVAGIYAPAVKD
CCCCCCCCCCEEEEEEEECCHHHHEECCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCC
ARLVVSPGDLIWVSATETTVTETNAKGRKEKKTIPFDPSTAKKGTPIALLLQQEPAVQGA
CEEEECCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHH
IASVEAENGDVVALIGGYQFGDSHFNRATQARRQPGSSFKPVVYSTALDMGFTAASTVLD
EEEEECCCCCEEEEECCEECCCHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCHHHHHHHC
APFVYVNPYTNEVWRPSNYEKNYKGELPLHQALTLSRNTCTVRIAQQVGIANVIQRAKAL
CCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCEEEEEHHHCCHHHHHHHHHHC
GLEPHFPQELSVSLGAVAVSPLNLTQAYAAFANKGLGVRPRIITSISDPQGRELYRQNVE
CCCCCCCHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHH
QWQAVTPQNAYIMATLLKQVVNAGTGSRARVQGFNIGGKTGTTNEEHDAWFVGFSPHLVT
HHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCHHHH
GVYVGYDQLQSLGRLEQGGRTAAPIFRYYRVEVEDRYPADDFAMPEGITMMDGLAFRTDM
EEEECHHHHHHHHHHHHCCCHHHCEEEEEEEEECCCCCCCCCCCCCCCCHHCCEEEECCC
PIQGVSATAATTEDDSVVDTSQGGEDLMRQMF
CCCCCCCCCCCCCCCCCEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA