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The map label for this gene is ilvI [H]

Identifier: 220905165

GI number: 220905165

Start: 2290937

End: 2292628

Strand: Direct

Name: ilvI [H]

Synonym: Ddes_1903

Alternate gene names: 220905165

Gene position: 2290937-2292628 (Clockwise)

Preceding gene: 220905164

Following gene: 220905166

Centisome position: 79.73

GC content: 59.28

Gene sequence:

>1692_bases
ATGGAATGTACAGGTGCGCAGATTCTCCTTGAGTCCCTGAAGAGAGAAGGCGTTGACGTGCTGTTCGGGTATCCCGGCGG
CGCTGTGATTGACATCTATGACGAACTGCCGCGACATCAAGAAATACGTCACGTGCTGGTACGACACGAACAAGGGGCTG
TGCACGCGGCTGACGGCTATGCCCGCGCTTCCGGCAAGGTAGGCGCCTGCCTGGTAACTTCCGGTCCCGGAGCCACCAAT
ACGGTGACGGGCATAGCCACAGCCTATTCCGATTCCATTCCTCTGGTAGTGATTACGGGGCAGGTTCCCACACAACTGAT
CGGCAACGATGCCTTTCAGGAAGTGGATATCGTGGGCATCACCCGGCCCTGTACCAAACACAATTTTCTGGTAAAGGACA
TCAGCAAGCTGGCCCTTACCATCCGTCAGGCTTTTTATCTGGCGCGCACGGGCCGCCCCGGCCCCGTACTCATTGACCTG
CCCAAAGACGTCATGCAGGCCAAGGCCGAGTTCGTGTGGCCTGAAGAAGTCTACATGCGCAGCTACAACCCCACCTACAA
GCCCAACCTCAACCAGTTGCGGCGCTCGGTAGAAGAGCTGGCCCGGGCGGAACGCCCGGTCATTCTGGCCGGGGGCGGGG
TTATCATGTCCGATGCGGCCGAGGCCCTCACGAGCCTTGCGCGCAAGCTGCAGATTCCCGTCACCTGTTCGCTCATGGGC
CTTGGGGCTTTTCCCGCCACCGACCCGCTGTGGCTCGGCATGGTCGGCATGCACGGAACCTATGCCGCCAATCTGGCCAT
CAACAATGCCGATGTGCTGGTATGCGTGGGCGCGCGCTTTGATGACCGCGTCACCGGAAAACTGGCGGGCTTCGCGCCCA
AGGCGCGCATTGTGCATATTGATATCGACCCCACATCCATACGCAAGAATGTGGAAGTACACGTTCCTGTGGTGGGCGAC
TGCCGCCTGGCGCTTGAAGGCATGGGCGAAATCTGCAACGCCAAGCTTGAAGGCAAAGACTGGAGCAGCGAACACGCCGC
GTGGCTTGAGGCCGTGGGCGAATGGAAGAAAAGCAAGCCTCTCTGCTACCAGAAAAACCACAACATCAAGCCGCAGGAAG
TCATCCAGACCCTGTACGAGCTTTCCAACGACGATGCCATCATTGCCACCGAAGTGGGCCAGCACCAGATGTGGGTAGCC
CAGTTCTATACGTTCACCAGGCCGCGCACCCTGCTCACCAGCGGAGGCCTCGGCACCATGGGGTACGGCTTTCCCGCGTC
CATCGGCGCACAGTTTGCCTTTCCCCACAAAAAAGTCATCACCGTCGCGGGCGACGCCTCATTGCAGATGAACATTCAGG
AGCTGGCTACCGTGGTTGCCAACAGGCTGCCCATCAAGGTGGTCATCCTGAACAACCGCCATCTCGGCATGGTGCGCCAG
TGGCAGGAGCTCTTTTACAACCAGAACTACAGCTCCACCAACATGGAGGCCCAGCCGGACTTTGTGAAGCTGGCCGAAGC
CTACGGGGCCGAAGGCTACCGCATTGAAAAACCCGAAGACCTGCGTGCCACGCTTGAAAAGGCCCTTGCCACACCCAACC
CGGCCTTTATCGACGTAGTGGTTGAACGCGAGGAAAACGTGTACCCCATCGTGCCTGCCGGCGCGGCGCTTGATGAAATG
TTGCTGGTGTAA

Upstream 100 bases:

>100_bases
GCAATTGAAAAAACAGAAGCTTGAAGGCAAAACCCAGCTCATGGCAATCCTCGACAGGATAAAAAAACAGGATTGCTAGC
AGGCTGTTAAGGAGCCGGCT

Downstream 100 bases:

>100_bases
AGGAGGGGCTATGACCAAGACACATGTGCTTTCCGTGCTGGTGGAAAATGAACCCGGCGTGCTTTCCCGCGTGGCGGGGC
TGTTCAGCGGGCGCGGCTTC

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MECTGAQILLESLKREGVDVLFGYPGGAVIDIYDELPRHQEIRHVLVRHEQGAVHAADGYARASGKVGACLVTSGPGATN
TVTGIATAYSDSIPLVVITGQVPTQLIGNDAFQEVDIVGITRPCTKHNFLVKDISKLALTIRQAFYLARTGRPGPVLIDL
PKDVMQAKAEFVWPEEVYMRSYNPTYKPNLNQLRRSVEELARAERPVILAGGGVIMSDAAEALTSLARKLQIPVTCSLMG
LGAFPATDPLWLGMVGMHGTYAANLAINNADVLVCVGARFDDRVTGKLAGFAPKARIVHIDIDPTSIRKNVEVHVPVVGD
CRLALEGMGEICNAKLEGKDWSSEHAAWLEAVGEWKKSKPLCYQKNHNIKPQEVIQTLYELSNDDAIIATEVGQHQMWVA
QFYTFTRPRTLLTSGGLGTMGYGFPASIGAQFAFPHKKVITVAGDASLQMNIQELATVVANRLPIKVVILNNRHLGMVRQ
WQELFYNQNYSSTNMEAQPDFVKLAEAYGAEGYRIEKPEDLRATLEKALATPNPAFIDVVVEREENVYPIVPAGAALDEM
LLV

Sequences:

>Translated_563_residues
MECTGAQILLESLKREGVDVLFGYPGGAVIDIYDELPRHQEIRHVLVRHEQGAVHAADGYARASGKVGACLVTSGPGATN
TVTGIATAYSDSIPLVVITGQVPTQLIGNDAFQEVDIVGITRPCTKHNFLVKDISKLALTIRQAFYLARTGRPGPVLIDL
PKDVMQAKAEFVWPEEVYMRSYNPTYKPNLNQLRRSVEELARAERPVILAGGGVIMSDAAEALTSLARKLQIPVTCSLMG
LGAFPATDPLWLGMVGMHGTYAANLAINNADVLVCVGARFDDRVTGKLAGFAPKARIVHIDIDPTSIRKNVEVHVPVVGD
CRLALEGMGEICNAKLEGKDWSSEHAAWLEAVGEWKKSKPLCYQKNHNIKPQEVIQTLYELSNDDAIIATEVGQHQMWVA
QFYTFTRPRTLLTSGGLGTMGYGFPASIGAQFAFPHKKVITVAGDASLQMNIQELATVVANRLPIKVVILNNRHLGMVRQ
WQELFYNQNYSSTNMEAQPDFVKLAEAYGAEGYRIEKPEDLRATLEKALATPNPAFIDVVVEREENVYPIVPAGAALDEM
LLV
>Mature_563_residues
MECTGAQILLESLKREGVDVLFGYPGGAVIDIYDELPRHQEIRHVLVRHEQGAVHAADGYARASGKVGACLVTSGPGATN
TVTGIATAYSDSIPLVVITGQVPTQLIGNDAFQEVDIVGITRPCTKHNFLVKDISKLALTIRQAFYLARTGRPGPVLIDL
PKDVMQAKAEFVWPEEVYMRSYNPTYKPNLNQLRRSVEELARAERPVILAGGGVIMSDAAEALTSLARKLQIPVTCSLMG
LGAFPATDPLWLGMVGMHGTYAANLAINNADVLVCVGARFDDRVTGKLAGFAPKARIVHIDIDPTSIRKNVEVHVPVVGD
CRLALEGMGEICNAKLEGKDWSSEHAAWLEAVGEWKKSKPLCYQKNHNIKPQEVIQTLYELSNDDAIIATEVGQHQMWVA
QFYTFTRPRTLLTSGGLGTMGYGFPASIGAQFAFPHKKVITVAGDASLQMNIQELATVVANRLPIKVVILNNRHLGMVRQ
WQELFYNQNYSSTNMEAQPDFVKLAEAYGAEGYRIEKPEDLRATLEKALATPNPAFIDVVVEREENVYPIVPAGAALDEM
LLV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=25.531914893617, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI21361361, Length=555, Percent_Identity=25.7657657657658, Blast_Score=155, Evalue=6e-38,
Organism=Escherichia coli, GI87081685, Length=565, Percent_Identity=51.5044247787611, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI1790104, Length=559, Percent_Identity=43.1127012522361, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1786717, Length=574, Percent_Identity=35.0174216027875, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1787096, Length=552, Percent_Identity=28.0797101449275, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=27.5179856115108, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17531299, Length=569, Percent_Identity=27.2407732864675, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=569, Percent_Identity=27.2407732864675, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=583, Percent_Identity=25.2144082332762, Blast_Score=145, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=589, Percent_Identity=45.5008488964346, Blast_Score=500, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6320816, Length=495, Percent_Identity=24.040404040404, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323163, Length=536, Percent_Identity=24.0671641791045, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=547, Percent_Identity=22.6691042047532, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323073, Length=496, Percent_Identity=24.5967741935484, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320123, Length=541, Percent_Identity=22.550831792976, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI19922626, Length=567, Percent_Identity=27.1604938271605, Blast_Score=184, Evalue=2e-46,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61541; Mature: 61541

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MECTGAQILLESLKREGVDVLFGYPGGAVIDIYDELPRHQEIRHVLVRHEQGAVHAADGY
CCCCHHHHHHHHHHHCCCEEEEECCCCCEEEHHHHCCCHHHHHHHHHHCCCCCEECCCCC
ARASGKVGACLVTSGPGATNTVTGIATAYSDSIPLVVITGQVPTQLIGNDAFQEVDIVGI
EECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEEECCCCHHHHCCCCCCEEEEEEE
TRPCTKHNFLVKDISKLALTIRQAFYLARTGRPGPVLIDLPKDVMQAKAEFVWPEEVYMR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCHHHHHHC
SYNPTYKPNLNQLRRSVEELARAERPVILAGGGVIMSDAAEALTSLARKLQIPVTCSLMG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHCCCCEEEEEEE
LGAFPATDPLWLGMVGMHGTYAANLAINNADVLVCVGARFDDRVTGKLAGFAPKARIVHI
CCCCCCCCCHHEEEECCCCEEEEEEEECCCCEEEEECCCCCCCCCCHHCCCCCCCEEEEE
DIDPTSIRKNVEVHVPVVGDCRLALEGMGEICNAKLEGKDWSSEHAAWLEAVGEWKKSKP
ECCHHHHCCCCEEEEEEECCHHHHHHCCHHHHCCEECCCCCCHHHHHHHHHHHHHHCCCC
LCYQKNHNIKPQEVIQTLYELSNDDAIIATEVGQHQMWVAQFYTFTRPRTLLTSGGLGTM
CEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCC
GYGFPASIGAQFAFPHKKVITVAGDASLQMNIQELATVVANRLPIKVVILNNRHLGMVRQ
CCCCCHHCCCEECCCCCEEEEEECCCCEEECHHHHHHHHHCCCCEEEEEECCCCCHHHHH
WQELFYNQNYSSTNMEAQPDFVKLAEAYGAEGYRIEKPEDLRATLEKALATPNPAFIDVV
HHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCCCCEEEEE
VEREENVYPIVPAGAALDEMLLV
EECCCCEEEEECCCHHHHHHHCC
>Mature Secondary Structure
MECTGAQILLESLKREGVDVLFGYPGGAVIDIYDELPRHQEIRHVLVRHEQGAVHAADGY
CCCCHHHHHHHHHHHCCCEEEEECCCCCEEEHHHHCCCHHHHHHHHHHCCCCCEECCCCC
ARASGKVGACLVTSGPGATNTVTGIATAYSDSIPLVVITGQVPTQLIGNDAFQEVDIVGI
EECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEEECCCCHHHHCCCCCCEEEEEEE
TRPCTKHNFLVKDISKLALTIRQAFYLARTGRPGPVLIDLPKDVMQAKAEFVWPEEVYMR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCHHHHHHC
SYNPTYKPNLNQLRRSVEELARAERPVILAGGGVIMSDAAEALTSLARKLQIPVTCSLMG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHCCCCEEEEEEE
LGAFPATDPLWLGMVGMHGTYAANLAINNADVLVCVGARFDDRVTGKLAGFAPKARIVHI
CCCCCCCCCHHEEEECCCCEEEEEEEECCCCEEEEECCCCCCCCCCHHCCCCCCCEEEEE
DIDPTSIRKNVEVHVPVVGDCRLALEGMGEICNAKLEGKDWSSEHAAWLEAVGEWKKSKP
ECCHHHHCCCCEEEEEEECCHHHHHHCCHHHHCCEECCCCCCHHHHHHHHHHHHHHCCCC
LCYQKNHNIKPQEVIQTLYELSNDDAIIATEVGQHQMWVAQFYTFTRPRTLLTSGGLGTM
CEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCC
GYGFPASIGAQFAFPHKKVITVAGDASLQMNIQELATVVANRLPIKVVILNNRHLGMVRQ
CCCCCHHCCCEECCCCCEEEEEECCCCEEECHHHHHHHHHCCCCEEEEEECCCCCHHHHH
WQELFYNQNYSSTNMEAQPDFVKLAEAYGAEGYRIEKPEDLRATLEKALATPNPAFIDVV
HHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCCCCEEEEE
VEREENVYPIVPAGAALDEMLLV
EECCCCEEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10555290 [H]