Click here to switch to the map view.

The map label for this gene is pdxT [H]

Identifier: 220905159

GI number: 220905159

Start: 2287101

End: 2287676

Strand: Reverse

Name: pdxT [H]

Synonym: Ddes_1897

Alternate gene names: 220905159

Gene position: 2287676-2287101 (Counterclockwise)

Preceding gene: 220905160

Following gene: 220905158

Centisome position: 79.61

GC content: 63.02

Gene sequence:

>576_bases
ATGGCCCGCTGTGTGGGTGTGCTGGCCCTTCAGGGGGCTTTTCGTGAGCATGTGGCCGCCGTGTCCCGTTTGGGCGTGGC
GGCCCGCGAAGTGCGCCAGCTCAAGGATATGGACGGCATTGACGCCATGATCATTCCGGGCGGCGAAAGCACCACCATGG
GCAAACTGCTCAACGAGTGGCAGATGCTGCAGCCGTTACGCGAGCGCATTGAGCAGGGCATGCCGGTATACGGCAGCTGC
GCCGGACTTATCCTGCTGTGCCGCGTTATTGAAAATTCGGACCAGCCCCGGCTGGGCGTGCTGGATGCCACCGTGCGGCG
CAACGCTTTCGGCCGTCAGGTTGACAGCTTTGAAACCGATCTCGCCATGCCGGAAATCGGGCCGGAACCTGTCCCGGCGG
TCTTCATCCGTGCTCCTGTCATCACTGGCGTGGGGCCGGGCGTCAAGGTGCTGGCCGAGGTCAAGGGGCAGGCTGTGGCG
GTGCGCCAGAACAATATTCTGGCCACGTCCTTTCATCCTGAGCTGACGCCGGATACGCGTCTGCATGGCTACTTTCTGGG
CATGTGCGGTAGCTGA

Upstream 100 bases:

>100_bases
CCTGCTGGCCGAAATCTCCCGTGATCTTGGCGAACCTATGGTGGGTATTGAAATTTCCACCATTCCCTCTGGTGAGCGCA
TGCAGGAGCGGGGCTGGTAG

Downstream 100 bases:

>100_bases
GGCAAAAACATCCTGCCGAAAGGGCGGCTTCCGGCGGCATACAGGCCGTCGTGCAGGCCCGGTATGTGGATGCTTTGAAC
ATGCGGCAAGATAGTCCGCA

Product: SNO glutamine amidotransferase

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT [H]

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MARCVGVLALQGAFREHVAAVSRLGVAAREVRQLKDMDGIDAMIIPGGESTTMGKLLNEWQMLQPLRERIEQGMPVYGSC
AGLILLCRVIENSDQPRLGVLDATVRRNAFGRQVDSFETDLAMPEIGPEPVPAVFIRAPVITGVGPGVKVLAEVKGQAVA
VRQNNILATSFHPELTPDTRLHGYFLGMCGS

Sequences:

>Translated_191_residues
MARCVGVLALQGAFREHVAAVSRLGVAAREVRQLKDMDGIDAMIIPGGESTTMGKLLNEWQMLQPLRERIEQGMPVYGSC
AGLILLCRVIENSDQPRLGVLDATVRRNAFGRQVDSFETDLAMPEIGPEPVPAVFIRAPVITGVGPGVKVLAEVKGQAVA
VRQNNILATSFHPELTPDTRLHGYFLGMCGS
>Mature_190_residues
ARCVGVLALQGAFREHVAAVSRLGVAAREVRQLKDMDGIDAMIIPGGESTTMGKLLNEWQMLQPLRERIEQGMPVYGSCA
GLILLCRVIENSDQPRLGVLDATVRRNAFGRQVDSFETDLAMPEIGPEPVPAVFIRAPVITGVGPGVKVLAEVKGQAVAV
RQNNILATSFHPELTPDTRLHGYFLGMCGS

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS [H]

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=205, Percent_Identity=42.9268292682927, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323995, Length=212, Percent_Identity=38.2075471698113, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321048, Length=212, Percent_Identity=38.6792452830189, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002161
- InterPro:   IPR021196 [H]

Pfam domain/function: PF01174 SNO [H]

EC number: NA

Molecular weight: Translated: 20605; Mature: 20474

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS51130 PDXT_SNO_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARCVGVLALQGAFREHVAAVSRLGVAAREVRQLKDMDGIDAMIIPGGESTTMGKLLNEW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHH
QMLQPLRERIEQGMPVYGSCAGLILLCRVIENSDQPRLGVLDATVRRNAFGRQVDSFETD
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCHHHHHHHH
LAMPEIGPEPVPAVFIRAPVITGVGPGVKVLAEVKGQAVAVRQNNILATSFHPELTPDTR
CCCCCCCCCCCCEEEEECCHHCCCCCHHHHHHHHCCCEEEEECCCEEEECCCCCCCCCCC
LHGYFLGMCGS
CCEEEEEECCC
>Mature Secondary Structure 
ARCVGVLALQGAFREHVAAVSRLGVAAREVRQLKDMDGIDAMIIPGGESTTMGKLLNEW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHH
QMLQPLRERIEQGMPVYGSCAGLILLCRVIENSDQPRLGVLDATVRRNAFGRQVDSFETD
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCHHHHHHHH
LAMPEIGPEPVPAVFIRAPVITGVGPGVKVLAEVKGQAVAVRQNNILATSFHPELTPDTR
CCCCCCCCCCCCEEEEECCHHCCCCCHHHHHHHHCCCEEEEECCCEEEECCCCCCCCCCC
LHGYFLGMCGS
CCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA