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The map label for this gene is cooF [H]

Identifier: 220905149

GI number: 220905149

Start: 2275057

End: 2276217

Strand: Direct

Name: cooF [H]

Synonym: Ddes_1887

Alternate gene names: 220905149

Gene position: 2275057-2276217 (Clockwise)

Preceding gene: 220905148

Following gene: 220905153

Centisome position: 79.18

GC content: 60.64

Gene sequence:

>1161_bases
ATGACTGAACATATCCAGGTCGTACCCGACAAATGCCGCGCCTGTCGCCGCTGTGAAGTGGCCTGTATTGCCGCCCACCA
CGGCATGAGTTTCAAAGAGGCCATGAAGCATCGCGATGAACTGGTATCGCGCGTGCAGGTGGTCAAGGCTGAAGGCTTCA
AAACCACCGTGCGCTGTCACCAGTGCCCCCATGCTCCCTGCGTCAATGTGTGCCCCACCGGGGCGCTGCAACAGGATGAG
CAGGGGCGCATCATCATGCGCGTCCAGTATTGCGTGGCCTGCAAAATGTGTATGGCGGCCTGCCCCTACGGAACCATCAC
TATGGAAACCATCGGCATGCCCACGGTAGACGGCGAAGACGGCGAAACCATCGCCCAGCGCGCCCGCCGCGAAGTAGCCG
TGCGTTGCGACATGTGCCGCGCGTGGCGGATGGAAAACGGCAAACGCATTACCGCCTGCATGGAAGCCTGCCCCGCCCAC
GCCCTCTCGCTGGTGCTGGCCGACGGCACGGTTGTGGAAGCACCCAAGCCGGAAAAGAAGGCTGTGGCCGCCGCAGCCAG
ACCAGAGGCTCCCAAGGCTGAAACAACCCCGGAAAACGGCACACAAGCTGCCGCAGGCACCGAAACCGCAGCCGCAGAAA
AAAGCATATCTGCCGCCAGTGAGCAAGAAATTTCCGTAACATCGGCCACGCCCGCTCCCCAGACGGCATCCGCCGCTGCA
GCCGCACCCGTGGCGGAGAAAAAAGATCCTGCGCCCGAAGCCGAAAAGGCAGACGCCGCCGAAGCTGAAAAAATATCCGT
CACCGAAACCGCCCCTACTGCAAAGGCAAAGGCCGATGAGCCTTCCGCCGGGCAGGTACAGGCGGATGAAACTCCCGCTG
CGGAAAATGCAGAAGATGCCGCGTTCTCTCCCGCCGCAACGGCCAAAGACAAACCCGTAAGCGCTCCGAAAACCGCTCCC
CAGGCCCCTGCCGAGCCTACTGGATCCCATGCTGCCGCCAAGGTTGAAGAAGCCCAGCAAACTGTAAAATCCCCGGCCCC
CGCGACAGCGGAAAAAAAGCCCGCAGCCGTAACAACGGTTGAAAAAAAATCCGCAGCCGCAGCAAAAAAACCTGCGGCGA
AGGGCAGCAAAAAAACGGCAAAAAAGAGCGGCAAAAAATAG

Upstream 100 bases:

>100_bases
CATTTCGTCTGTCCCAGTTGCGGCGGCGAGAACATCCACTTCAACGGGGGCCACGGACTTACCCTGATGGCCCTGCACGT
TGCATCCGAGGAAACGGACC

Downstream 100 bases:

>100_bases
GGCAGGCCATCAGGGCTGCGGATGCCCTGAAAAATAGCGCTGCCCTGAAAAAACCAGAAAGGCGGACCAGCCCGCCCCCC
AAATACACGAGGCCCGTTCG

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTEHIQVVPDKCRACRRCEVACIAAHHGMSFKEAMKHRDELVSRVQVVKAEGFKTTVRCHQCPHAPCVNVCPTGALQQDE
QGRIIMRVQYCVACKMCMAACPYGTITMETIGMPTVDGEDGETIAQRARREVAVRCDMCRAWRMENGKRITACMEACPAH
ALSLVLADGTVVEAPKPEKKAVAAAARPEAPKAETTPENGTQAAAGTETAAAEKSISAASEQEISVTSATPAPQTASAAA
AAPVAEKKDPAPEAEKADAAEAEKISVTETAPTAKAKADEPSAGQVQADETPAAENAEDAAFSPAATAKDKPVSAPKTAP
QAPAEPTGSHAAAKVEEAQQTVKSPAPATAEKKPAAVTTVEKKSAAAAKKPAAKGSKKTAKKSGKK

Sequences:

>Translated_386_residues
MTEHIQVVPDKCRACRRCEVACIAAHHGMSFKEAMKHRDELVSRVQVVKAEGFKTTVRCHQCPHAPCVNVCPTGALQQDE
QGRIIMRVQYCVACKMCMAACPYGTITMETIGMPTVDGEDGETIAQRARREVAVRCDMCRAWRMENGKRITACMEACPAH
ALSLVLADGTVVEAPKPEKKAVAAAARPEAPKAETTPENGTQAAAGTETAAAEKSISAASEQEISVTSATPAPQTASAAA
AAPVAEKKDPAPEAEKADAAEAEKISVTETAPTAKAKADEPSAGQVQADETPAAENAEDAAFSPAATAKDKPVSAPKTAP
QAPAEPTGSHAAAKVEEAQQTVKSPAPATAEKKPAAVTTVEKKSAAAAKKPAAKGSKKTAKKSGKK
>Mature_385_residues
TEHIQVVPDKCRACRRCEVACIAAHHGMSFKEAMKHRDELVSRVQVVKAEGFKTTVRCHQCPHAPCVNVCPTGALQQDEQ
GRIIMRVQYCVACKMCMAACPYGTITMETIGMPTVDGEDGETIAQRARREVAVRCDMCRAWRMENGKRITACMEACPAHA
LSLVLADGTVVEAPKPEKKAVAAAARPEAPKAETTPENGTQAAAGTETAAAEKSISAASEQEISVTSATPAPQTASAAAA
APVAEKKDPAPEAEKADAAEAEKISVTETAPTAKAKADEPSAGQVQADETPAAENAEDAAFSPAATAKDKPVSAPKTAPQ
APAEPTGSHAAAKVEEAQQTVKSPAPATAEKKPAAVTTVEKKSAAAAKKPAAKGSKKTAKKSGKK

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH). CooF is required in stoichiometric amounts in vitro for anchoring CODH to the membrane as well as

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367245, Length=162, Percent_Identity=38.8888888888889, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789067, Length=180, Percent_Identity=35.5555555555556, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI87082179, Length=165, Percent_Identity=36.3636363636364, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788811, Length=165, Percent_Identity=34.5454545454545, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI87082180, Length=157, Percent_Identity=34.3949044585987, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI2367345, Length=137, Percent_Identity=32.8467153284672, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082114, Length=204, Percent_Identity=28.921568627451, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI226510944, Length=124, Percent_Identity=31.4516129032258, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787872, Length=210, Percent_Identity=27.1428571428571, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787122, Length=201, Percent_Identity=26.3681592039801, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 40083; Mature: 39951

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHIQVVPDKCRACRRCEVACIAAHHGMSFKEAMKHRDELVSRVQVVKAEGFKTTVRCH
CCCCEEECCHHHHHHHHCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHH
QCPHAPCVNVCPTGALQQDEQGRIIMRVQYCVACKMCMAACPYGTITMETIGMPTVDGED
CCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
GETIAQRARREVAVRCDMCRAWRMENGKRITACMEACPAHALSLVLADGTVVEAPKPEKK
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEECCEEEECCCCHHH
AVAAAARPEAPKAETTPENGTQAAAGTETAAAEKSISAASEQEISVTSATPAPQTASAAA
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHH
AAPVAEKKDPAPEAEKADAAEAEKISVTETAPTAKAKADEPSAGQVQADETPAAENAEDA
HCCCCCCCCCCCCCHHCCCCCHHEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCC
AFSPAATAKDKPVSAPKTAPQAPAEPTGSHAAAKVEEAQQTVKSPAPATAEKKPAAVTTV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
EKKSAAAAKKPAAKGSKKTAKKSGKK
HHHHHHHHHCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
TEHIQVVPDKCRACRRCEVACIAAHHGMSFKEAMKHRDELVSRVQVVKAEGFKTTVRCH
CCCEEECCHHHHHHHHCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHH
QCPHAPCVNVCPTGALQQDEQGRIIMRVQYCVACKMCMAACPYGTITMETIGMPTVDGED
CCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
GETIAQRARREVAVRCDMCRAWRMENGKRITACMEACPAHALSLVLADGTVVEAPKPEKK
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEECCEEEECCCCHHH
AVAAAARPEAPKAETTPENGTQAAAGTETAAAEKSISAASEQEISVTSATPAPQTASAAA
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHH
AAPVAEKKDPAPEAEKADAAEAEKISVTETAPTAKAKADEPSAGQVQADETPAAENAEDA
HCCCCCCCCCCCCCHHCCCCCHHEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCC
AFSPAATAKDKPVSAPKTAPQAPAEPTGSHAAAKVEEAQQTVKSPAPATAEKKPAAVTTV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
EKKSAAAAKKPAAKGSKKTAKKSGKK
HHHHHHHHHCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644755 [H]