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The map label for this gene is trmD [H]

Identifier: 220905052

GI number: 220905052

Start: 2162570

End: 2163877

Strand: Direct

Name: trmD [H]

Synonym: Ddes_1789

Alternate gene names: 220905052

Gene position: 2162570-2163877 (Clockwise)

Preceding gene: 220905051

Following gene: 220905053

Centisome position: 75.26

GC content: 60.47

Gene sequence:

>1308_bases
ATGCCGCGTTTTCATATTGTTTCGCTCTTTCCCGATTTTTTCAGTTCGCCCCTGTCAACGGCCCTTATGGGACGCGCGCG
CGAGGCGGGCCTTGTGGATTTTTCTTTTCATGATCCGCGCGATTTCAGCACCAGCAAACACCGCCACGTGGACGACCGCC
CTTACGGCGGCGGGCCTGGCATGGTCATGCAGGGTGAACCCCTGGCCGGAGCGTTGCGGTCCATTGAAAGCCCGGGACGC
ATCCTGCTCATGGCTCCCGGCGGCCGCCCCTTTACCCAGGACACGGCGCGGCAGCTGGCCGAAGAGCAGGACCTGACCAT
TATCTGCGGGCGCTATGAAGGCATTGACGCCCGCTTGCTGGATATTTTTGCCATTGAACCCACAAGCGTTGGCGATGTGG
TGCTTAATGGCGGCGAAACGGCGGCTTTGGCCGTTGTGGAGGCCGTTGCGCGCCTTATGCCCGGCTTTATGGGCAAGGAA
GAATCCGGTGAGGACGAAAGCTTTTCGAACGGCCTGCTGGAATATCCGCACTACACCCGGCCTGAGACACTGGAAGGCCT
GCCCGTCCCCCAGGTGCTGCTTGGCGGTGACCATGCCCGCATAGCGCAATGGCGGCGGCGGCAGTCCGTGCAGACAACGC
TGCGCATGCGACCCGACCTGCTCGACTCTGCCCCGCTTACCGGCGAAGACGTGCAGACGCTGGCTGAAATTCCGCGGGAA
AGGCCGGGGCGCAACCTTTCTTTCTGTCTTGTGCACTATCCTGTGGTGCTCGGCGAAAAAAAATCCGGCGCTTCCTCTTT
GACAAATCTCGACATACACGATATAGCCCGAATTTCCCGCAGCTATGCGATGGGTTCTTTCCATGTGGTAACCCCCTTGC
AAGACCAGTTGCGGGTGCTGGATGAAATTTTGCGCCATTGGACGCAGGGTCCGGGCGGTTCAGGTAATGCCGACCGGGCG
CAGGCTCTCGGTCTGGTTTGCCCTGCGGCCTCCCTGGATGAAGCAGTGGCGCATATGACCGCACGGCACGGTGTACGGCC
CAGGCTGGTGGCAAGCTCCGCCGTCTGGCCCGGCAAGGGTAAAAAGCAGGCTCGTACAGAACCGCTCATGACCCCTCAAC
AGGTGCGGCAATGGTGCAGACAGGGACCGGTCATGCTTTGCCTTGGCACAGCGCGGGGGCTTGCCTCCGAAGTGCTTGAA
CAGTGTGACGGTCTGCTTCGTCCTGTGCGTTTTTTGGGCTACAATCACCTTTCGGTGCGCAGCGCCGCCGCCATTCTGGC
AGACAGAATTTTAGGCGACTATTGCTGA

Upstream 100 bases:

>100_bases
GCGCCCTTGCGGCAGACCCGTCCGGCAAAACGGCAGAAGCAGTTTCCGATCCCGCAATCCTTTCCTTGCAAGGCGGGGCG
ATTTGCGGCACTCTCCGCCC

Downstream 100 bases:

>100_bases
TAAACAGGCGGCCTGAGCGCATCGGCGCCGCTACGCAACGACGCAACAATCCGCCCGGATTTGCAAGGAGTTCACGATGG
ATATCATCAAAAAGATTGAA

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MPRFHIVSLFPDFFSSPLSTALMGRAREAGLVDFSFHDPRDFSTSKHRHVDDRPYGGGPGMVMQGEPLAGALRSIESPGR
ILLMAPGGRPFTQDTARQLAEEQDLTIICGRYEGIDARLLDIFAIEPTSVGDVVLNGGETAALAVVEAVARLMPGFMGKE
ESGEDESFSNGLLEYPHYTRPETLEGLPVPQVLLGGDHARIAQWRRRQSVQTTLRMRPDLLDSAPLTGEDVQTLAEIPRE
RPGRNLSFCLVHYPVVLGEKKSGASSLTNLDIHDIARISRSYAMGSFHVVTPLQDQLRVLDEILRHWTQGPGGSGNADRA
QALGLVCPAASLDEAVAHMTARHGVRPRLVASSAVWPGKGKKQARTEPLMTPQQVRQWCRQGPVMLCLGTARGLASEVLE
QCDGLLRPVRFLGYNHLSVRSAAAILADRILGDYC

Sequences:

>Translated_435_residues
MPRFHIVSLFPDFFSSPLSTALMGRAREAGLVDFSFHDPRDFSTSKHRHVDDRPYGGGPGMVMQGEPLAGALRSIESPGR
ILLMAPGGRPFTQDTARQLAEEQDLTIICGRYEGIDARLLDIFAIEPTSVGDVVLNGGETAALAVVEAVARLMPGFMGKE
ESGEDESFSNGLLEYPHYTRPETLEGLPVPQVLLGGDHARIAQWRRRQSVQTTLRMRPDLLDSAPLTGEDVQTLAEIPRE
RPGRNLSFCLVHYPVVLGEKKSGASSLTNLDIHDIARISRSYAMGSFHVVTPLQDQLRVLDEILRHWTQGPGGSGNADRA
QALGLVCPAASLDEAVAHMTARHGVRPRLVASSAVWPGKGKKQARTEPLMTPQQVRQWCRQGPVMLCLGTARGLASEVLE
QCDGLLRPVRFLGYNHLSVRSAAAILADRILGDYC
>Mature_434_residues
PRFHIVSLFPDFFSSPLSTALMGRAREAGLVDFSFHDPRDFSTSKHRHVDDRPYGGGPGMVMQGEPLAGALRSIESPGRI
LLMAPGGRPFTQDTARQLAEEQDLTIICGRYEGIDARLLDIFAIEPTSVGDVVLNGGETAALAVVEAVARLMPGFMGKEE
SGEDESFSNGLLEYPHYTRPETLEGLPVPQVLLGGDHARIAQWRRRQSVQTTLRMRPDLLDSAPLTGEDVQTLAEIPRER
PGRNLSFCLVHYPVVLGEKKSGASSLTNLDIHDIARISRSYAMGSFHVVTPLQDQLRVLDEILRHWTQGPGGSGNADRAQ
ALGLVCPAASLDEAVAHMTARHGVRPRLVASSAVWPGKGKKQARTEPLMTPQQVRQWCRQGPVMLCLGTARGLASEVLEQ
CDGLLRPVRFLGYNHLSVRSAAAILADRILGDYC

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=239, Percent_Identity=44.7698744769874, Blast_Score=211, Evalue=8e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019230
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF09936 DUF2168; PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 47493; Mature: 47362

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFHIVSLFPDFFSSPLSTALMGRAREAGLVDFSFHDPRDFSTSKHRHVDDRPYGGGPG
CCCEEEEEECHHHHCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
MVMQGEPLAGALRSIESPGRILLMAPGGRPFTQDTARQLAEEQDLTIICGRYEGIDARLL
CEEECCCHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEE
DIFAIEPTSVGDVVLNGGETAALAVVEAVARLMPGFMGKEESGEDESFSNGLLEYPHYTR
EEEEECCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCC
PETLEGLPVPQVLLGGDHARIAQWRRRQSVQTTLRMRPDLLDSAPLTGEDVQTLAEIPRE
CCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHCHHH
RPGRNLSFCLVHYPVVLGEKKSGASSLTNLDIHDIARISRSYAMGSFHVVTPLQDQLRVL
CCCCCCEEEEEEEEEEECCCCCCCCHHCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHH
DEILRHWTQGPGGSGNADRAQALGLVCPAASLDEAVAHMTARHGVRPRLVASSAVWPGKG
HHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
KKQARTEPLMTPQQVRQWCRQGPVMLCLGTARGLASEVLEQCDGLLRPVRFLGYNHLSVR
CCCCCCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SAAAILADRILGDYC
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRFHIVSLFPDFFSSPLSTALMGRAREAGLVDFSFHDPRDFSTSKHRHVDDRPYGGGPG
CCEEEEEECHHHHCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
MVMQGEPLAGALRSIESPGRILLMAPGGRPFTQDTARQLAEEQDLTIICGRYEGIDARLL
CEEECCCHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEE
DIFAIEPTSVGDVVLNGGETAALAVVEAVARLMPGFMGKEESGEDESFSNGLLEYPHYTR
EEEEECCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCC
PETLEGLPVPQVLLGGDHARIAQWRRRQSVQTTLRMRPDLLDSAPLTGEDVQTLAEIPRE
CCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHCHHH
RPGRNLSFCLVHYPVVLGEKKSGASSLTNLDIHDIARISRSYAMGSFHVVTPLQDQLRVL
CCCCCCEEEEEEEEEEECCCCCCCCHHCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHH
DEILRHWTQGPGGSGNADRAQALGLVCPAASLDEAVAHMTARHGVRPRLVASSAVWPGKG
HHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
KKQARTEPLMTPQQVRQWCRQGPVMLCLGTARGLASEVLEQCDGLLRPVRFLGYNHLSVR
CCCCCCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SAAAILADRILGDYC
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA