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The map label for this gene is fusA [H]

Identifier: 220905048

GI number: 220905048

Start: 2155325

End: 2157388

Strand: Direct

Name: fusA [H]

Synonym: Ddes_1785

Alternate gene names: 220905048

Gene position: 2155325-2157388 (Clockwise)

Preceding gene: 220905046

Following gene: 220905049

Centisome position: 75.01

GC content: 59.06

Gene sequence:

>2064_bases
ATGCCTACCCCACTGGAAATGCAACGCACCTTTGCACTCGTCGGCACCGGCGGCTGCGGCAAAACCTCTCTTGCCGAGAT
GCTGCTGTTCAATGCCGGAACCACCACCCGCATGGGCGCCATTGAAGAAGGCACCACAAGCCTCGACTACGAGCCGGAAG
AAACCCGGCGGCGCGGCTCGGTGCAGCCCGCCTGCGCGACCTATATGTGGAACAAAAACCGGCATTTCCTGCTGGATATC
CCTGGTGACGGCAACTTTACGGGCGATATGGAATGCCTGCTCAAGGGTGTGGACAGCGTGGTTTTTGTGCTGGACGCCGT
GGACGGTGTGCGGCCCCTGGCAAAAAAATTCTGGAGCATGGTCCGGCAGGCCGGTCTGCCCGTCATGATCGTGATCAATA
AGCTTGACCGCGACCGCGCCGACTTTCAGATGACCTTTGACGGCCTGTCCGCCCTGGGCATCCGGCCTGTGGCCCTGCAA
CTGCCCATCCGCCAGGGTGAAACATTTTTGGGTGTGGCCGATGTGCTCTCCGGCAAGGCACACATGTTCAAGCCCGACGG
CAGCACGGCTGAAGCCGAAATTCCTGCCGGGCTTGCCGATGATGTGAGCCTTTTACGCGATATTACCGTGGAAAATATCG
CTGAAAGCGATGAAATCCTTATGGAAAAGTATCTTGAAGAAGGCAGCCTGCCACTGGAAGACCTGCACGACGGCCTGCGT
GCGGGCGTTATCAAGGGAGAGCTGATTCCCGTCCTGACCTGCTCGGCCCTTGAAAACAAGGGCGGTGCGGCCTTGCTTGA
TGCCATAGAAGCCCTGCTGCCCTCGCCTCTTGACCGCCCGGCCTTTGAAGGTGAAGGCGGCGCCAGCCGTACCGCTGACC
CGGAAGGTCCGGTGGCCTGCTTTGTGTTCAAGACCCTGGCCGACCCCTTTGCCGGGCAGCTCTCCCTGCTGCGGATTCTT
TCCGGCACCATCAGTGGCGATGTAAACCTTAAAAACATGCGCAGCGAAGACAATGAGCGGCTGGGCAGCCTGCTGTTCCT
GACGGGCAAAAACCAGACCCCCTGCAAGGAACCGATAGGCCCCGGCGCCATTGTGGCTGTGGCCAAACTCAAAAACACCC
GCACAGGCGACACCCTTTGCGATGAAAAGGCCCCCTTTGTGCTGCCCATGCCACACCTGCCGCCGCAACTCATTACCTAC
GCCCTGGCTCCCAGGGAAAAAGGCGAAGAGGACAAGGTTTACGCGGCCATGCAGCGGCTGCTGGACGAAGATATTACCCT
GAAGCTCGGCCGTGACGGAGAAAACGGCGATATCCTGCTCTCGGGCATGGGGCAGTTGCATATCGAGCTTTCTGTTGAAA
AGGCCAAGCGCCGCTTCAAGGTGGATATCGTGCTCAAGACCCCCAAGGTTCCCTACCGCGAAACAGTGCGCGGCAAGTGT
CAGGTCCAGGGCAGGCACAAAAAGCAGTCCGGTGGCCGGGGCCAGTTCGGCGACTGCTGGATAGAAATGGAAGGGTTGCC
GCGCGGCTCCGGTTACCAGTTTGAAGATGCCATTGTGGGCGGCGTCATCCCCCGCCAGTACGTTCCCGCCATTGACAAGG
GCGTGCAGGAAGCTGCGGCGCGGGGTTTTCTGGCCGGATGCCAGGTAGTGGATTTCCGCGTCAAGGTTTATGACGGCAGC
TATCACACTGTGGACTCTTCAGAAATGGCCTTCAAGGTGGCAGGCTCACTGGCGTTCAAAAAGGCTATGGAGAATCTCAA
GCCGGTTTTGCTGGAACCCATTGTGCTGCTGACGGTTTCTGTTCCCGATGAAAGCATGGGCGACGTTATCGGCGACCTCT
CCTCCCGCAGGGGCAAGGTGCTTGGCTCCGATTCGCAGGGCGGCAGCACAGAAATCAAGGCCCATGTACCCATGAGCGAA
GTGCTGCGCTACGCTCCCGACCTGCGCTCCATGACTGGAGGGCAGGGCTTTTTCACTATGGAATTTGCCCACTATGAAGA
AGCCCCGCAGCCTGTAACAGAAAAAATTGTGGCTGAATACCAGGGCGCAAAGGCCGCCGAGTAA

Upstream 100 bases:

>100_bases
TCCCCTGCCCCGCCTTCGCAACACCGTGCTCCTTCCCTGGTTTCGTTCACCCGGCCCGCGCCGCGCGCGCCAGCCACATC
GCTTACTAAGGGAGAAAACC

Downstream 100 bases:

>100_bases
TCACAGTCTTCCCTGCCGGACCGCCACGTGTATTCAGAAACCGGCGAACCGGCAGGGCTTTCGCCACAACCCTCCGGACG
CGCAGGCCTTTTGTCCTGCC

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MPTPLEMQRTFALVGTGGCGKTSLAEMLLFNAGTTTRMGAIEEGTTSLDYEPEETRRRGSVQPACATYMWNKNRHFLLDI
PGDGNFTGDMECLLKGVDSVVFVLDAVDGVRPLAKKFWSMVRQAGLPVMIVINKLDRDRADFQMTFDGLSALGIRPVALQ
LPIRQGETFLGVADVLSGKAHMFKPDGSTAEAEIPAGLADDVSLLRDITVENIAESDEILMEKYLEEGSLPLEDLHDGLR
AGVIKGELIPVLTCSALENKGGAALLDAIEALLPSPLDRPAFEGEGGASRTADPEGPVACFVFKTLADPFAGQLSLLRIL
SGTISGDVNLKNMRSEDNERLGSLLFLTGKNQTPCKEPIGPGAIVAVAKLKNTRTGDTLCDEKAPFVLPMPHLPPQLITY
ALAPREKGEEDKVYAAMQRLLDEDITLKLGRDGENGDILLSGMGQLHIELSVEKAKRRFKVDIVLKTPKVPYRETVRGKC
QVQGRHKKQSGGRGQFGDCWIEMEGLPRGSGYQFEDAIVGGVIPRQYVPAIDKGVQEAAARGFLAGCQVVDFRVKVYDGS
YHTVDSSEMAFKVAGSLAFKKAMENLKPVLLEPIVLLTVSVPDESMGDVIGDLSSRRGKVLGSDSQGGSTEIKAHVPMSE
VLRYAPDLRSMTGGQGFFTMEFAHYEEAPQPVTEKIVAEYQGAKAAE

Sequences:

>Translated_687_residues
MPTPLEMQRTFALVGTGGCGKTSLAEMLLFNAGTTTRMGAIEEGTTSLDYEPEETRRRGSVQPACATYMWNKNRHFLLDI
PGDGNFTGDMECLLKGVDSVVFVLDAVDGVRPLAKKFWSMVRQAGLPVMIVINKLDRDRADFQMTFDGLSALGIRPVALQ
LPIRQGETFLGVADVLSGKAHMFKPDGSTAEAEIPAGLADDVSLLRDITVENIAESDEILMEKYLEEGSLPLEDLHDGLR
AGVIKGELIPVLTCSALENKGGAALLDAIEALLPSPLDRPAFEGEGGASRTADPEGPVACFVFKTLADPFAGQLSLLRIL
SGTISGDVNLKNMRSEDNERLGSLLFLTGKNQTPCKEPIGPGAIVAVAKLKNTRTGDTLCDEKAPFVLPMPHLPPQLITY
ALAPREKGEEDKVYAAMQRLLDEDITLKLGRDGENGDILLSGMGQLHIELSVEKAKRRFKVDIVLKTPKVPYRETVRGKC
QVQGRHKKQSGGRGQFGDCWIEMEGLPRGSGYQFEDAIVGGVIPRQYVPAIDKGVQEAAARGFLAGCQVVDFRVKVYDGS
YHTVDSSEMAFKVAGSLAFKKAMENLKPVLLEPIVLLTVSVPDESMGDVIGDLSSRRGKVLGSDSQGGSTEIKAHVPMSE
VLRYAPDLRSMTGGQGFFTMEFAHYEEAPQPVTEKIVAEYQGAKAAE
>Mature_686_residues
PTPLEMQRTFALVGTGGCGKTSLAEMLLFNAGTTTRMGAIEEGTTSLDYEPEETRRRGSVQPACATYMWNKNRHFLLDIP
GDGNFTGDMECLLKGVDSVVFVLDAVDGVRPLAKKFWSMVRQAGLPVMIVINKLDRDRADFQMTFDGLSALGIRPVALQL
PIRQGETFLGVADVLSGKAHMFKPDGSTAEAEIPAGLADDVSLLRDITVENIAESDEILMEKYLEEGSLPLEDLHDGLRA
GVIKGELIPVLTCSALENKGGAALLDAIEALLPSPLDRPAFEGEGGASRTADPEGPVACFVFKTLADPFAGQLSLLRILS
GTISGDVNLKNMRSEDNERLGSLLFLTGKNQTPCKEPIGPGAIVAVAKLKNTRTGDTLCDEKAPFVLPMPHLPPQLITYA
LAPREKGEEDKVYAAMQRLLDEDITLKLGRDGENGDILLSGMGQLHIELSVEKAKRRFKVDIVLKTPKVPYRETVRGKCQ
VQGRHKKQSGGRGQFGDCWIEMEGLPRGSGYQFEDAIVGGVIPRQYVPAIDKGVQEAAARGFLAGCQVVDFRVKVYDGSY
HTVDSSEMAFKVAGSLAFKKAMENLKPVLLEPIVLLTVSVPDESMGDVIGDLSSRRGKVLGSDSQGGSTEIKAHVPMSEV
LRYAPDLRSMTGGQGFFTMEFAHYEEAPQPVTEKIVAEYQGAKAAE

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=702, Percent_Identity=31.7663817663818, Blast_Score=305, Evalue=1e-82,
Organism=Homo sapiens, GI19923640, Length=707, Percent_Identity=27.015558698727, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI25306287, Length=684, Percent_Identity=26.1695906432749, Blast_Score=206, Evalue=7e-53,
Organism=Homo sapiens, GI25306283, Length=391, Percent_Identity=31.2020460358056, Blast_Score=149, Evalue=9e-36,
Organism=Escherichia coli, GI1789738, Length=697, Percent_Identity=38.7374461979914, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1790835, Length=477, Percent_Identity=24.5283018867925, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI48994988, Length=173, Percent_Identity=30.0578034682081, Blast_Score=71, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17533571, Length=699, Percent_Identity=28.6123032904149, Blast_Score=297, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17556745, Length=701, Percent_Identity=23.39514978602, Blast_Score=146, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6323098, Length=704, Percent_Identity=30.8238636363636, Blast_Score=303, Evalue=5e-83,
Organism=Saccharomyces cerevisiae, GI6322359, Length=792, Percent_Identity=25.1262626262626, Blast_Score=217, Evalue=5e-57,
Organism=Drosophila melanogaster, GI24582462, Length=701, Percent_Identity=32.2396576319544, Blast_Score=327, Evalue=2e-89,
Organism=Drosophila melanogaster, GI221458488, Length=709, Percent_Identity=26.2341325811001, Blast_Score=199, Evalue=8e-51,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 74333; Mature: 74202

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPLEMQRTFALVGTGGCGKTSLAEMLLFNAGTTTRMGAIEEGTTSLDYEPEETRRRGS
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCC
VQPACATYMWNKNRHFLLDIPGDGNFTGDMECLLKGVDSVVFVLDAVDGVRPLAKKFWSM
CCCHHHHEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VRQAGLPVMIVINKLDRDRADFQMTFDGLSALGIRPVALQLPIRQGETFLGVADVLSGKA
HHHCCCCEEEEEECCCCCCCCEEEEECCHHHCCCCEEEEEEECCCCCEEEEHHHHHCCCC
HMFKPDGSTAEAEIPAGLADDVSLLRDITVENIAESDEILMEKYLEEGSLPLEDLHDGLR
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHH
AGVIKGELIPVLTCSALENKGGAALLDAIEALLPSPLDRPAFEGEGGASRTADPEGPVAC
HCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEE
FVFKTLADPFAGQLSLLRILSGTISGDVNLKNMRSEDNERLGSLLFLTGKNQTPCKEPIG
EEHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHCCCCHHHCCCEEEEEECCCCCCCCCCCC
PGAIVAVAKLKNTRTGDTLCDEKAPFVLPMPHLPPQLITYALAPREKGEEDKVYAAMQRL
CCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHEEECCCCCCCHHHHHHHHHHH
LDEDITLKLGRDGENGDILLSGMGQLHIELSVEKAKRRFKVDIVLKTPKVPYRETVRGKC
HCCCCEEEECCCCCCCCEEEECCCEEEEEEEEHHHCCEEEEEEEEECCCCCHHHCCCCEE
QVQGRHKKQSGGRGQFGDCWIEMEGLPRGSGYQFEDAIVGGVIPRQYVPAIDKGVQEAAA
EECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
RGFLAGCQVVDFRVKVYDGSYHTVDSSEMAFKVAGSLAFKKAMENLKPVLLEPIVLLTVS
HHHHHCCEEEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEEEE
VPDESMGDVIGDLSSRRGKVLGSDSQGGSTEIKAHVPMSEVLRYAPDLRSMTGGQGFFTM
CCCHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEECCHHHHHHHCCCHHHCCCCCCEEEE
EFAHYEEAPQPVTEKIVAEYQGAKAAE
EEHHHCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PTPLEMQRTFALVGTGGCGKTSLAEMLLFNAGTTTRMGAIEEGTTSLDYEPEETRRRGS
CCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCC
VQPACATYMWNKNRHFLLDIPGDGNFTGDMECLLKGVDSVVFVLDAVDGVRPLAKKFWSM
CCCHHHHEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VRQAGLPVMIVINKLDRDRADFQMTFDGLSALGIRPVALQLPIRQGETFLGVADVLSGKA
HHHCCCCEEEEEECCCCCCCCEEEEECCHHHCCCCEEEEEEECCCCCEEEEHHHHHCCCC
HMFKPDGSTAEAEIPAGLADDVSLLRDITVENIAESDEILMEKYLEEGSLPLEDLHDGLR
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHH
AGVIKGELIPVLTCSALENKGGAALLDAIEALLPSPLDRPAFEGEGGASRTADPEGPVAC
HCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEE
FVFKTLADPFAGQLSLLRILSGTISGDVNLKNMRSEDNERLGSLLFLTGKNQTPCKEPIG
EEHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHCCCCHHHCCCEEEEEECCCCCCCCCCCC
PGAIVAVAKLKNTRTGDTLCDEKAPFVLPMPHLPPQLITYALAPREKGEEDKVYAAMQRL
CCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHEEECCCCCCCHHHHHHHHHHH
LDEDITLKLGRDGENGDILLSGMGQLHIELSVEKAKRRFKVDIVLKTPKVPYRETVRGKC
HCCCCEEEECCCCCCCCEEEECCCEEEEEEEEHHHCCEEEEEEEEECCCCCHHHCCCCEE
QVQGRHKKQSGGRGQFGDCWIEMEGLPRGSGYQFEDAIVGGVIPRQYVPAIDKGVQEAAA
EECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
RGFLAGCQVVDFRVKVYDGSYHTVDSSEMAFKVAGSLAFKKAMENLKPVLLEPIVLLTVS
HHHHHCCEEEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEEEE
VPDESMGDVIGDLSSRRGKVLGSDSQGGSTEIKAHVPMSEVLRYAPDLRSMTGGQGFFTM
CCCHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEECCHHHHHHHCCCHHHCCCCCCEEEE
EFAHYEEAPQPVTEKIVAEYQGAKAAE
EEHHHCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA