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The map label for this gene is shiA [H]

Identifier: 220905045

GI number: 220905045

Start: 2151709

End: 2153082

Strand: Direct

Name: shiA [H]

Synonym: Ddes_1782

Alternate gene names: 220905045

Gene position: 2151709-2153082 (Clockwise)

Preceding gene: 220905042

Following gene: 220905046

Centisome position: 74.88

GC content: 58.01

Gene sequence:

>1374_bases
ATGTCAGACGCAAGGGCACAAAAACACGTTCCCACCAGACAGGAGATGCGCAAGGTCGTTATCGCCAGCCTGCTCGGCGC
GACCATCGAATGGTACGACTTTTTCCTCTACGGGGTCGTGGCGGGCATTGTGTTCAACAAGCTTTACTTTCCCACCACCG
ACCCTTTCATCGGAACCATCCTCGCTTACAGCACCTTCGCCATCGGTTATCTGGCCCGTCCCCTCGGGGGCTTCATATTC
GGGCACTACGGTGACAAGCTGGGCCGCAAGCGCATGCTTATCCTCACCATGCTGATTATGGGCATAGCCACGGTGGGCAT
CGGCCTTATCCCCACCTATGCCAGTATCGGCATTGCCGCCCCCATACTGCTGCAAACCCTGCGCCTGTGCCAGGGGCTTG
GCCTTGGCGGCGAATGGGGCGGAGCGGTACTTATGACCTATGAATACGCCAGCGAACGCGAAAAAGCCTTTTATGCCAGT
ATTCCCCAGATGGGGCTGGCTACGGGCCTTTGCCTTGCTTCCGGCATGGTGGCCCTGCTTTCCTGGCTTTTGACCAACGA
ACAGTTCATGGCCTGGGGCTGGCGCCTGGCTTTTCTCATGAGTGTTGTGCTGATAGCCATAGCGCTCTACGTACGGACCC
ATATTCTTGAAACCCCCGAATTTCGCAAGGTGCAGGAAGAAGGTCAGGCTGAAAAGAAAGTTCTGCCCATTGTCACGGTA
TGCAAGAACTTTCCGGGCAATATAGCCCTCGGCGTTGGCGCGCGCTGGATTGACGGCGTGTTCTTTAACGTGCTGGCGGT
TTTTTCCATCAGTTATCTTGTGCAGTACATAGACGTCACGCGCACCCAGGCCCTGACTGCGGTCATGATCGCGGCCCTGC
TCATGTGCCCGTTCATCCTCATCGCCGGACGCCTGGCCGACCGCTTCGGCCGAGGCCGCATTTATGGTCTGGCAAGCCTG
GCCTGCGCCCTGACCATCTTTCCTTCTTTCTGGCTCATGCAGGGCAGCGGCGGAAACATATTCATCATCGGCCTTGCCAT
AGCCATTCCCCTCAGCATCTGTTATGCCGGGGTGTTTGGGCCTGAAGCCGCACTTTTTTCTGATCTTTTTCCGGCTGAAG
TGCGCTACACCGGCATTTCCATAGTGTACCAGTTTCCCGGATTTCTGGTGGCGGGCATCGTACCCGGCGTGTGCACGGCG
CTTATGCGGTGGAACGACGGTAATCCGGCCTATATCTGCATTTTTGTGATCATTGCGGCTGTCACCAGCGCCCTGTCGGC
CTTTGCCATCCAGCGGCGGCATGACAGGGCGGCACGGCAGGCTCAGGCCCATGGCGTCATGCAGGCCGGGGCAAGCTCGC
AGGCTCAGGGGTAA

Upstream 100 bases:

>100_bases
ATATTTAATAGATAAAACAAAATACTCCTGCACGCAATTAAAACTTTTAATAGCCGCACGACAGGAAGCCCCTGGCGGGA
CGGCAGCCCCGGAGGAAAGC

Downstream 100 bases:

>100_bases
CCATGCATATACTACAACTTTCCGATACGCATCTACGCGGCGACAACAGCCTGTCCTTCCGGGTTGTGGATACGCGCCGC
TGTCTTGATGCCGCCACCGC

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSDARAQKHVPTRQEMRKVVIASLLGATIEWYDFFLYGVVAGIVFNKLYFPTTDPFIGTILAYSTFAIGYLARPLGGFIF
GHYGDKLGRKRMLILTMLIMGIATVGIGLIPTYASIGIAAPILLQTLRLCQGLGLGGEWGGAVLMTYEYASEREKAFYAS
IPQMGLATGLCLASGMVALLSWLLTNEQFMAWGWRLAFLMSVVLIAIALYVRTHILETPEFRKVQEEGQAEKKVLPIVTV
CKNFPGNIALGVGARWIDGVFFNVLAVFSISYLVQYIDVTRTQALTAVMIAALLMCPFILIAGRLADRFGRGRIYGLASL
ACALTIFPSFWLMQGSGGNIFIIGLAIAIPLSICYAGVFGPEAALFSDLFPAEVRYTGISIVYQFPGFLVAGIVPGVCTA
LMRWNDGNPAYICIFVIIAAVTSALSAFAIQRRHDRAARQAQAHGVMQAGASSQAQG

Sequences:

>Translated_457_residues
MSDARAQKHVPTRQEMRKVVIASLLGATIEWYDFFLYGVVAGIVFNKLYFPTTDPFIGTILAYSTFAIGYLARPLGGFIF
GHYGDKLGRKRMLILTMLIMGIATVGIGLIPTYASIGIAAPILLQTLRLCQGLGLGGEWGGAVLMTYEYASEREKAFYAS
IPQMGLATGLCLASGMVALLSWLLTNEQFMAWGWRLAFLMSVVLIAIALYVRTHILETPEFRKVQEEGQAEKKVLPIVTV
CKNFPGNIALGVGARWIDGVFFNVLAVFSISYLVQYIDVTRTQALTAVMIAALLMCPFILIAGRLADRFGRGRIYGLASL
ACALTIFPSFWLMQGSGGNIFIIGLAIAIPLSICYAGVFGPEAALFSDLFPAEVRYTGISIVYQFPGFLVAGIVPGVCTA
LMRWNDGNPAYICIFVIIAAVTSALSAFAIQRRHDRAARQAQAHGVMQAGASSQAQG
>Mature_456_residues
SDARAQKHVPTRQEMRKVVIASLLGATIEWYDFFLYGVVAGIVFNKLYFPTTDPFIGTILAYSTFAIGYLARPLGGFIFG
HYGDKLGRKRMLILTMLIMGIATVGIGLIPTYASIGIAAPILLQTLRLCQGLGLGGEWGGAVLMTYEYASEREKAFYASI
PQMGLATGLCLASGMVALLSWLLTNEQFMAWGWRLAFLMSVVLIAIALYVRTHILETPEFRKVQEEGQAEKKVLPIVTVC
KNFPGNIALGVGARWIDGVFFNVLAVFSISYLVQYIDVTRTQALTAVMIAALLMCPFILIAGRLADRFGRGRIYGLASLA
CALTIFPSFWLMQGSGGNIFIIGLAIAIPLSICYAGVFGPEAALFSDLFPAEVRYTGISIVYQFPGFLVAGIVPGVCTAL
MRWNDGNPAYICIFVIIAAVTSALSAFAIQRRHDRAARQAQAHGVMQAGASSQAQG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=435, Percent_Identity=37.9310344827586, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1789941, Length=407, Percent_Identity=39.8034398034398, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1790550, Length=439, Percent_Identity=31.8906605922551, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI1788942, Length=439, Percent_Identity=28.0182232346241, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87082231, Length=418, Percent_Identity=23.2057416267943, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082404, Length=388, Percent_Identity=24.2268041237113, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49541; Mature: 49410

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDARAQKHVPTRQEMRKVVIASLLGATIEWYDFFLYGVVAGIVFNKLYFPTTDPFIGTI
CCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LAYSTFAIGYLARPLGGFIFGHYGDKLGRKRMLILTMLIMGIATVGIGLIPTYASIGIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PILLQTLRLCQGLGLGGEWGGAVLMTYEYASEREKAFYASIPQMGLATGLCLASGMVALL
HHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
SWLLTNEQFMAWGWRLAFLMSVVLIAIALYVRTHILETPEFRKVQEEGQAEKKVLPIVTV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHH
CKNFPGNIALGVGARWIDGVFFNVLAVFSISYLVQYIDVTRTQALTAVMIAALLMCPFIL
HCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAGRLADRFGRGRIYGLASLACALTIFPSFWLMQGSGGNIFIIGLAIAIPLSICYAGVFG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEHHHHHHHHHHHHHHCCC
PEAALFSDLFPAEVRYTGISIVYQFPGFLVAGIVPGVCTALMRWNDGNPAYICIFVIIAA
CHHHHHHHCCCCCEEECCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VTSALSAFAIQRRHDRAARQAQAHGVMQAGASSQAQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]